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Allosteric Adenosine Modulation to Reduce Allodynia Hui-Lin Pan, Ph.D.,* Zemin Xu, B.S.,† Edward Leung, Ph.D.,‡ James C

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Allosteric Adenosine Modulation to Reduce Allodynia Hui-Lin Pan, Ph.D.,* Zemin Xu, B.S.,† Edward Leung, Ph.D.,‡ James C Anesthesiology 2001; 95:416–20 © 2001 American Society of Anesthesiologists, Inc. Lippincott Williams & Wilkins, Inc. Allosteric Adenosine Modulation to Reduce Allodynia Hui-Lin Pan, Ph.D.,* Zemin Xu, B.S.,† Edward Leung, Ph.D.,‡ James C. Eisenach, M.D.§ Background: Adenosine and adenosine agonists reduce hy- Considerable recent work has demonstrated a remark- persensitivity following inflammation and peripheral nerve in- able neuronal plasticity associated with peripheral nerve jury models of chronic pain. Because inhibitors of adenosine injury, which may underlie hypersensitivity. This is re- reuptake or metabolism are also effective at reducing hypersen- sitivity, it is likely that there is a tonic release of spinal adeno- flected in the loss of efficacy of some classes of tradi- 1 sine in these models. One approach to avoid adverse effects tional agents, such as opioids; increased efficacy of ␣ from direct agonists is to enhance the effect of the endogenous some classes of analgesics, such as 2-adrenergic ago- ligand by administering a positive allosteric modulator of its nists;2 and expression of efficacy of agents that are not Downloaded from http://pubs.asahq.org/anesthesiology/article-pdf/95/2/416/332592/0000542-200108000-00025.pdf by guest on 27 September 2021 receptor. analgesic in normal animals, such as the tricyclic antide- Methods: Rats with mechanical hypersensitivity after spinal 3 nerve ligation received intrathecal injections of adenosine, the pressants or gabapentin. Adenosine is an example of allosteric adenosine receptor modulator T62, or their combina- the latter group in that intrathecal injection of adenosine tion, or received systemic T62 alone or with intrathecal injec- has little or no effect in the normal animal but reduces tion of a specific A1 adenosine antagonist. mechanical hypersensitivity after surgery or spinal nerve Results: Both adenosine and T62 reduced hypersensitivity ligation in rats.4,5 Pharmacologic studies are consistent ؎ alone, with 50% maximal doses (ED50)of14 5.9 and ␮ with an action of adenosine on spinal A1 receptors to ؎ 3.7 0.8 g, respectively. They interacted in an additive man- 6 ner as determined by isobolography. T62 also reduced produce this effect. mechanical hypersensitivity after systemic administration A common problem with administration of direct ago- (15 mg/kg), and this effect was blocked by intrathecal injec- nists, like adenosine, are adverse effects caused by stim- tion of 9 ␮g of the A1-specific adenosine receptor antagonist ulation of all receptors, not just those involved in the 8-cyclopentyl-1, 3-dipropylxanthine. therapeutic effect. One approach to obviate this prob- Conclusions: These results add to previous studies that sug- gest ongoing spinal release of adenosine, which is antiallo- lem is the use of positive allosteric modulators, as exem- dynic, in this animal model of neuropathic pain. Positive plified by the use of benzodiazepines to enhance the allosteric modulation of the adenosine receptor reduces hy- effect of ␥-aminobutyric acid. Because we previously persensitivity by a spinal mechanism involving A1 adenosine observed evidence for tonic spinal adenosine release receptor stimulation. Although obvious adverse effects were after spinal nerve ligation,5 the purpose of the current not observed in this investigation, further study is required to determine the feasibility of the use of such modulators in the study was to test the efficacy and mechanism of action of treatment of chronic pain associated with hyperalgesia and a positive allosteric modulator of adenosine receptors in allodynia. this model of hypersensitivity. PERIPHERAL nerve injury and inflammation can result in chronic pain associated with hypersensitivity to nor- Methods mally nonpainful or painful stimuli, termed allodynia and Animal Preparation hyperalgesia, respectively. These elicited pains are fre- After Animal Care and Use Committee approval, me- quently difficult to treat with standard analgesics, and chanical hypersensitivity was generated in male Sprague- the response to nontraditional agents, such as antide- Dawley rats as previously described.7 Briefly, under gen- pressants, local anesthetics, and antiepileptics, is often eral anesthesia with inhalational halothane, the left L5 incomplete or not realizable because of a high incidence and L6 spinal nerves were identified through a small of adverse effects. For this reason, there is a need for laminotomy and were tightly ligated. Approximately 1 development of novel and effective analgesics for these week later, a polyethylene-10 intrathecal catheter was types of pain. placed under general anesthesia by insertion under di- rect vision of a polyethylene catheter through a small slit in the dura at the cisterna magnum and advanced 8.5 cm * Associate Professor of Anesthesiology, † Research Technician, § Francis M. such that the catheter tip resided in the lower lumbar James, III Professor of Anesthesiology, Department of Anesthesiology, Wake intrathecal space. Proper location was confirmed by bi- Forest University School of Medicine. ‡ Director, Preclinical Research and De- velopment, Medco Research, Inc. lateral motor blockade after injection of 5 ␮l lidocaine, Received from the Pain Mechanisms Laboratory, Department of Anesthesiol- 2%. Animals were studied approximately 1 week later. ogy, Wake Forest University School of Medicine, Winston-Salem, North Carolina, Ligation of the lumbar spinal nerves results in a pri- and Medco Research, Inc., Cary, North Carolina. Submitted for publication September 21, 2000. Accepted for publication January 3, 2001. Supported in part marily unilateral increase in the sensitivity to light touch by Medco Research, Inc., Cary, North Carolina, and grant No. GM35523 from the on the side of surgery. Sensitivity was assessed via ap- National Institutes of Health, Bethesda, Maryland. Address correspondence to Dr. Eisenach: Professor of Anesthesiology, Wake plication of calibrated von Frey filaments. The with- Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, drawal threshold was determined using an up–down North Carolina 27157-1009. Address electronic mail to: [email protected]. 8 Reprints will not be available from the authors. Individual article reprints may be method previously described. In general, withdrawal purchased through the Journal Web site, www.anesthesiology.org. threshold is 35 g or greater in animals before surgery, Anesthesiology, V 95, No 2, Aug 2001 416 ALLOSTERIC ADENOSINE MODULATOR AND ANALGESIA 417 which is markedly reduced to less than4gwithin 1 Data Analysis week of spinal nerve ligation, and this hypersensitivity is Data are presented as median Ϯ 25th and 75th percen- stable for several weeks thereafter. tiles (for raw withdrawal thresholds) or by mean Ϯ SE. Linear regression was used to calculate the ED50 for each drug alone and for the fixed ratio combination. The ED Drug Treatment 50 was determined for each animal rather than using a Animals were randomized to receive a single intrathe- probit analysis of the entire data set. The effect of each cal injection of 1, 3, or 5 ␮g of the allosteric adenosine agent alone and the combination on withdrawal thresh- receptor modulator T62 or 5, 10, or 30 ␮g adenosine. old over time was tested by Kruskal-Wallis analysis fol- Doses of T62 and adenosine were determined in pilot lowed by Wilcoxon rank sum test. P Ͻ 0.05 was consid- experiments or from previously completed studies to ered significant. Downloaded from http://pubs.asahq.org/anesthesiology/article-pdf/95/2/416/332592/0000542-200108000-00025.pdf by guest on 27 September 2021 encompass the therapeutically effective range. Animals received six injections by the same individual, each sep- arated by a minimum of 4 days to prevent development of tolerance. The withdrawal threshold to mechanical Results stimulation with von Frey filaments was determined be- Intrathecal Administration Studies fore and at intervals for 3 h after intrathecal injection and Intrathecal injection of T62 produced dose- and time- then again at 20 h after injection. A dose response was dependent increases in withdrawal threshold, returning determined using the time of peak effect (2 h for each sensitivity to preligation baseline (35 g, 25th and 75th compound), and the ED50 was determined by linear percentiles ϭ 32.2 and 38.7 g) after the 5-␮g dose (fig. 1, regression. molecular structure inset, top). The ED50 of T62 was To determine the type of interaction between intrathe- 3.7 Ϯ 0.8 ␮g. Similarly, intrathecal adenosine produced cal adenosine and T62, an isobolographic approach was a dose- and time-dependent increase in withdrawal used. In this design, adenosine and T62 were combined threshold, returning sensitivity to preligation baseline at in the ratio of their ED s, and this combination was ␮ 50 the 30- g dose (fig. 1, middle). The ED50 of adenosine administered in different doses in a constant ratio (ad- was 14 Ϯ 5.9 ␮g, similar to that which we previously ␮ 5 enosine:T62 ratio of 7:1.8, 14:3.5, or 24:6 g). The ED50 reported. Based on these results, adenosine and T62 for the mixture (total of each component combined) were combined in a 4:1 ratio. This combination also was determined by linear regression, an isobologram produced dose- and time-dependent increases in with- was constructed, and the observed ED50 was compared drawal threshold, returning it to preligation baseline at with the theoretical ED by a t test as previously ␮ 50 the 30- g combined dose (fig. 1, bottom). The ED50 of 9 described. this combination was 15 Ϯ 2.9 ␮g (12 Ϯ 2.3 ␮g adeno- To determine the site of action of T62, two experi- sine plus 3 Ϯ 0.6 ␮g T62). Injection of vehicle, either ments were performed. First, the effect of intraperito- saline or cyclodextrin, had no effect on withdrawal neal injection of T62, 15 mg/kg, was tested and was threshold (data not shown). Adenosine, 30 ␮g, resulted noted to be effective to reduce hypersensitivity (see in sedation, as evidenced by lack of exploration of an Results). The effect of systemic T62 was then tested 5 open environment, but no abnormal gait indicating mo- min after intrathecal injection of the A1 receptor-prefer- tor blockade.
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