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The Biology of RNA-Protein Complexes

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The Biology of RNA-Protein Complexes The Biology of RNA-Protein Complexes PROGRAM AND ABSTRACTS October11thͲ14th2017,ThonͲDittmerPalais,Regensburg,Germany GENERAL INFORMATION Throughout this program, the numbers next to the titles refer to corresponding oral or poster abstract numbers in the abstract section of this book. Please refrain from citing the abstracts in bibliographies. The information contained in this abstract book should be treated as personal communicationandshouldbecitedonlywiththeexpressedconsentoftheauthor. Nounauthorizedphotographyinsessions: To encourage the sharing of novel and unpublished data, taking photographs and/or videos during the scientific sessions (both oral andposters),orofpostersoutsidesessionhours,isstrictlyprohibited. Violatorsofthispolicymayhavetheirequipmentconfiscatedand/or theymaybeaskedtoleavetheconferenceandhavetheirregistration privilegesrevokedwithoutreimbursement Useofsocialmedia: Thehashtagis#SFB960.Weencourageallattendeestotweetabout the amazing science they experience at the SFB960 meeting in Regensburg.But,pleasedorespectthefollowingruleswhentweeting orusingothersocialmedia: 1. bepoliteandrespectfulofothersinallofyourmessages 2. donottransmitphotographsofslidesorpostersunderany circumstances 3. donottransmitphotographsofconferenceattendeeswithout theirexpressedconsent 4. tweetersshouldrespectrequestsofpresenterswhoasktorefrain fromtweetingthecontentoftheirtalksorposters CoverPicture:TobiasBeck Ͳ Layout:JanMedenbach Ͳ Print: Offset Haas 2 MEETING SPONSORS 3 SCIENTIFIC ORGANIZERS Prof. Dr. Thomas Dresselhaus Cell Biology and Plant Biochemistry University of Regensburg [email protected] Prof. Dr. Dina Grohmann Microbiology & Single Molecule Biochemistry University of Regensburg [email protected] Dr. Jan Medenbach Biochemistry I University of Regensburg [email protected] Prof. Dr. Gunter Meister Biochemistry I University of Regensburg [email protected] Prof. Dr. Herbert Tschochner Biochemistry III University of Regensburg [email protected] 4 ABOUT THE SFB960 Ribonucleoproteins (RNPs) are cellular multicomponent assemblies whose biological functions dependontheinteractionbetweenRNAandproteins.ThedynamicprocessofRNPassemblyand regulationofRNPfunctionrepresentsthemaininterestoftheCollaborativeResearchCentreSFB960. TheSFB960issupportedbytheDeutscheForschungsgemeinschaft(DFG)sinceJuly2011establishing astronglocalscientificnetworkattheUniversityofRegensburg.Itcurrentlyunites13researchand two core/service projects focusing on biogenesis and regulation of RNPs which are all directly or indirectlyinvolvedinthesynthesisorregulationofribosomes.Multisubunitcomplexesinteracting with the rRNA synthesizing machineries, with argonauteͲcontaining complexes or with nascent or mature ribosomes are especially interesting. This enables comparative analyses, which will reveal commonprinciplesandspecificaspectsofimportantRNPͲrelatedprocesses. To further the knowledge of how ribosomes and other RNPs are synthesized and regulated, the SFB960 organizes international scientific meetings on a regular basis. The symposium 2017 "The biology of RNAͲprotein complexes" is already the third international conference organized by members of the SFB960, in which leading scientists of the RNP field exchange their thoughts and presenttheirongoingworkinahopefullystimulatingenvironment. 5 6 INDEX Schedule..................................................................................................................................................8 OralAbstracts........................................................................................................................................15 PosterAbstracts(AͲZ)............................................................................................................................63 ListofParticipants...............................................................................................................................134 VenueInformation&Maps.................................................................................................................154 7 SCHEDULE Wednesday,October11th 13:00 Registrationstarts:ThonͲDittmerͲPalais,1stfloor KeynoteLectures 15:30 WelcomeAddress 15:45 RoyParker:RNPGranulesinHealthandDisease 16:15 Reinhard Lührmann: CryoͲEM snapshots of the highly dynamic spliceosome 17:00 AlanHinnebusch:Anetworkofmolecularinteractionsrestrictsstable preinitiationcomplexassemblytooptimaltranslationstartcodonsin vivo 17:45 DinneratThonͲDittmerͲPalais 18:30 PostersandDrinks–atthe‘großerDollingersaal’ Evenposternumbers 8 Thursday,October12th Session:Transcription Sessionchair:DinaGrohmann 09:00 WelcomeAddress:Prof.Dr.UdoHebel,PresidentoftheUniversityof Regensburg 09:10 Evgeny Nudler: Structural Principles of TranscriptionͲcoupled DNA Repair 09:35 Stefan Knauer: Exploring RNA Polymerase Regulation by NMR spectroscopy 09:50 AlbertWeixlbaumer:RegulationofTranscriptionElongationbyNusA –biochemicalandstructuralstudies 10:05 DavidDulin:AnetworkofpausestrapsthebacterialRNApolymerase anditsnascenttranscriptonthepromoter 10:20 FinnWerner:MolecularMechanismsofTranscriptioninArchaea 10:45 coffeebreak 11:15 DylanTaatjes:NonͲcodingRNAs,Mediator,andMediatorkinases 11:40 Steven Hahn: Fuzzy FreeͲforͲAll: Retention of Fuzzy Binding in the MultiͲDomain Interactions of Transcription Activator Gcn4 and MediatorSubunitMed15 12:05 Zoltan Villanyi: Ccr4ͲNot is at the core of the gene expression circuitry 12:20 Christoph Müller: Structural biology of RNA polymerase I and III transcription 12:45 Lunchbreak 9 Session:RNAprocessing Sessionchair:JanMedenbach 14:00 JulianKönig:iCLIPͲbasedmodelinguncovers3’splicesitedefinition: howU2AF65specificityreliesonregulationbycoͲfactors 14:15 ŠtĢpánka VaŸáēová: N6Ͳmethyladenosine demethylase FTO targets preͲmRNAsandregulatesalternativesplicingand3’endprocessing 14:40 Michaela MüllerͲMcNicoll: Cellular differentiation state modulates themRNAexportandretentionactivitiesofSRproteins 15:05 DanielaLazzaretti:InsightsintospecificRNArecognitionmediatedby human Staufen1 from the crystal structure of its minimal RNAͲ bindingregionincomplexwithaphysiologicaldsRNAtarget 15:20 coffeebreak 15:50 Oliver Mühlemann: Dissecting the functions of SMG5, SMG7 and PNRC2innonsenseͲmediatedmRNAdecayofhumancells 16:15 RemcoSprangers:TheRrp4Ͳexosomecomplexrecruitsandchannels substrateRNAbyauniquemechanism 16:30 Michael Sattler: Decoding regulatory proteinͲRNA interactions in generegulationusingintegratedstructuralbiology 17:00 SponsoredSeminar: NicolasPiganeau Whole Transcriptome Analysis: Choosetherighttoolforthejob 17:30 Guidedtouroftheoldtown startingrightoutsidetheThonͲDittmerͲPalaisonHaidtplatz 19:00 ConferenceDinnerat‘HausHeuport’ 10 Friday,October13th Session:Ribosomebiogenesisandfunction Sessionchair:SébastienFereirraͲCercaandJorgePerezͲFernandez I–FundamentalaspectsofribosomebiogenesisinEukaryotes 09:00 Ed Hurt: Mechanism of ribosome biogenesis and its link to nucleocytoplasmictransport 09:25 Brigitte Pertschy: Coordination of distant 40S ribosomal subunit maturationevents 09:40 Susan J. Baserga: High throughput discovery of novel regulators of humanribosomebiogenesis 10:05 CameronMackereth:AnoveldsRNAͲbindingmodulefromanuclear prolylisomerase II–Ribosomebiogenesisanddisease 10:20 Nicholas Watkins: The 5S RNP provides the link between ribosome biogenesisandthetumoursuppressorp53 10:35 Antje OstareckͲLederer: Interaction of hnRNP K and rpS19 and its functioninerythroidmaturation 10:50 coffeebreak III–Ribosomefunction 11:20 DanielNWilson:RibosomeͲtargetingantibioticsandmechanismsof bacterialresistance 11:45 Axel Innis: Discovery and characterization of ribosomeͲarresting peptidesbyHighͲthroughputInverseToeprinting 12:00 Nenad Ban: Extensions, extra factors and extreme complexity: Ribosomalstructuresprovideinsightsintoeukaryotictranslation 12:25 KristynaPoncova:ThesmallribosomalproteinRPS3anditsfunctions 12:45 Lunchbreak 11 IV–Translationcontrolandqualitycontrol 13:45 GeorgAuburger:EfficientPreventionofNeurodegenerativeDiseases byDepletionofStarvationResponseFactorAtaxinͲ2 14:00 Nagammal Neelagandan: Mutant TDPͲ43 selectively affects translationofspecificmRNAsinculturedcorticalneuronsandmotor neuronͲlikecells 14:15 Keynote LectureͲNahum Sonenberg: Ribosome quality control of premature polyadenylated mRNAs by a unique E3 ubiquitin ligase andRNAͲbindingprotein 15:00 coffeebreak Session:nonͲcodingRNAs Sessionchair:MarkusKretz 15:30 LeemorJoshuaͲTor:MadaboutU:regulatingthelet7preͲmiRNA 15:55 Peter Sarnow: From bench to clinical trial: microRNA 122 as an antiviraltargetforhepatitisCvirus 16:20 ClausͲD.Kuhn:TheroleofpiRNAsinplanarianregeneration 16:35 SaraK.Eisenbart:AnickelͲregulatedsmallRNArepressesexpression ofmultiplemajorvirulencefactorsinHelicobacterpylori 16:50 LisaͲKatharina Maier: SelfͲtargeting and gene repression with the CRISPRͲCassystemofHaloferaxvolcanii 17:05 Markus T. Bohnsack: METTL16 is a N6Ͳmethyladenosine (m6A) methyltransferase that targets preͲmRNAs, lncRNAs and the U6 snRNA 17:20 SvenDiederichs:lncRNA&RibonucleoproteinsinCancer 17:45 UtzFischer:MechanisticdissectionofUsnRNPbiogenesisanditsrole indisease 18:10 DinneratThonͲDittmerͲPalais 18:45 PostersandDrinks–atthe‘großerDollingersaal’ Oddposternumbers 12 Saturday,October14th Session:RNAsystemsbiology Sessionchair:AndreaBleckmann 09:00 Vicent Pelechano: GenomeͲwide study of transcription complexity andribosomedynamics 09:25 Jörg Vogel: GradͲseq discovers the third domain of small RNAͲ mediatedgeneregulationinbacteria
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