bioRxiv preprint doi: https://doi.org/10.1101/816264; this version posted January 21, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Mechanism and site of action of big dynorphin on ASIC1a Borg, C.B.1#, Braun, N.1#, Heusser, S.A.1, Bay, Y.1, Weis, D.1, Galleano, I.1, Lund, C.1, Tian, W.2, Haugaard-Kedström, L.M.1, Bennett, E.P.2, Lynagh, T.1, Strømgaard, K.1, Andersen, J.3, Pless, S.A.1* 1Department of Drug Design and Pharmacology, University of Copenhagen, Jagtvej 160, 2100 Copenhagen, Denmark 2Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen, Denmark 3Vipergen ApS, Gammel Kongevej 23A, 1610 Copenhagen, Denmark # These authors contributed equally * Correspondence: Stephan A. Pless (
[email protected]) Center for Biopharmaceuticals, Department of Drug Design and Pharmacology University of Copenhagen, Jagtvej 160, 2100 Copenhagen, Denmark 1 bioRxiv preprint doi: https://doi.org/10.1101/816264; this version posted January 21, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Abstract Acid-sensing ion channels (ASICs) are proton-gated cation channels that contribute to neurotransmission, as well as initiation of pain and neuronal death following ischemic stroke. As such, there is a great interest in understanding the in vivo regulation of ASICs, especially by endogenous neuropeptides that potently modulate ASICs.