Glossary of Biotechnology Terms
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Cell Fusion Induced by Pederine
Pediat. Res. 8: 606-608 (1974) Cell fusion heterokaryon pederine Cell Fusion Induced by Pederine MAURAR. LEVINE,[~~]JOSEPH DANCIS,MARIO PAVAN, AND RODYP. COX Division of Human Genetics and the Departments of Pharmacology, Pediatrics and Medicine, New York University School of Medicine, New York, New York, USA, and the Institute of Entomology, University of Pavia, Pavia, Italy Extract Pederine, a natural product extracted from beetles, induces cell fusion among hu- man skin fibroblasts grown in tissue culture. Heterokaryons are produced when pederine is added to mixtures of human diploid fibroblasts and HeLa cells. The effi- ciency of cell fusion exceeds that achieved with other available agents. The technique is simple and the results are reproducible. Cells exposed to pederine under conditions that cause fusion retain their growth potential, which indicates that the treatment does not damage the cells. The technique should prove useful in research into mecha- nisms of membrane fusion, as well as research in which cell fusion is used as an in- vestigative tool. Speculation Lysolecithin is believed to induce cell fusion by perturbing the molecular structure of cellular membranes. Pederine is more effective at concentrations less than one thous- andth that of lysolecithin. The mechanism of pederine-induced cell fusion may pro- vide insight into the physiologic processes which maintain membrane integrity. Introduction years. The phenomenon of membrane fuslon is involved in a multitude of physiological processes including fertilization, pinocytosis and the forma- The experimental induction of cell Yusion among cells grown in tissue culture tion of syncytia. It is also a cmon event in pathological conditions such has facilitated studies of the mechanism of membrane fusion as well as re- as viral Infections and the response to foreign bodies. -
Prokaryotic Sex: Eukaryote-Like Qualities of Recombination in An
Dispatch R601 Prokaryotic Sex: Eukaryote-like match, the ends may be cut to a random extent by exonucleases, Qualities of Recombination in an and then the newly revealed ends are tested, and so on. This would increase Archaean Lineage the probability that eventually a reduced donor segment would sufficiently match a sequence from the Genetic exchange within one Archaean lineage is a bit like sex in recipient. We suggest this process eukaryotes — cells fuse and huge segments of DNA are recombined — with would be particularly successful in consequences for the spread of adaptations across species. organisms that recombine through cell fusion, as the donor segments start out Frederick M. Cohan* (which may be harmful to the recipient) exceptionally long. This hypothesis and Stephanie Aracena [3]. We therefore hypothesize that predicts that more-closely-related in Haloferax and other cell-fusion organisms may recombine after Two decades ago, Moshe Mevarech systems, niche-transcending a smaller number of cuts; so and colleagues discovered an adaptations may not transfer as easily more-distant crosses would yield extraordinary mode of recombination as in the Bacteria. On the other hand, shorter recombinant segments, in an Archaean taxon — cells of the huge size of recombined a pattern observed in Bacillus Haloferax can recombine through cell segments may foster the transfer transformation [3]. fusion [1]. After two cells fuse, their of extremely complex adaptations The authors suggest that horizontal genomes can recombine, and then the that could not otherwise be transferred genetic transfer would be particularly fused cell can resolve into two cells, [4], including possibly the ancient easy between species where cell fusion each with a single chromosome. -
Biotechnology: Answers to Common Questions
FSR0030 Biotechnology: Answers to Common Questions Kevin Keener, Assistant Professor of Food Science Thomas Hoban, Professor of Sociology and Food Science N.C. Cooperative Extension Service N.C. State University We are entering the “Century of Biology.” Recent developments in the biological sciences are giving us a better understanding of the natural world. At the same type we are developing new tools that are collectively referred to as “biotechnology.” These help us address problems related to human health, food production, and the environment. Any new technology – particularly one as far-reaching as biotechnology – will generate interest, as well as concerns. Because the science behind biotechnology is complex, misconceptions arise over its impacts and implications. In this publication we will answer questions many people have about biotechnology. These questions are organized along the lines of a news story: what, when, who, where, why, and how. We also provide a list of additional information sources available on the Internet. Our primary focus will be on the uses of biotechnology in agriculture and food production since these appear to be more controversial than other applications (at least up until this time). What is Biotechnology? In its broadest sense, biotechnology refers to the use of living systems to develop products. New scientific discoveries are allowing us to better understand fundamental life processes at the cellular and molecular level. Now we can improve selected attributes of microbes, plants, or animals for human use by making precise genetic changes that were not possible with traditional methods. All living organisms contain genes that carry the hereditary traits between generations. -
An Overview of Molecular Events Occurring in Human Trophoblast Fusion Pascale Gerbaud, Guillaume Pidoux
An overview of molecular events occurring in human trophoblast fusion Pascale Gerbaud, Guillaume Pidoux To cite this version: Pascale Gerbaud, Guillaume Pidoux. An overview of molecular events occurring in human trophoblast fusion. Placenta, Elsevier, 2015, 36 (Suppl1), pp.S35-42. 10.1016/j.placenta.2014.12.015. inserm- 02556112v2 HAL Id: inserm-02556112 https://www.hal.inserm.fr/inserm-02556112v2 Submitted on 28 Apr 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. 1 An overview of molecular events occurring in human trophoblast fusion 2 3 Pascale Gerbaud1,2 & Guillaume Pidoux1,2,† 4 1INSERM, U1139, Paris, F-75006 France; 2Université Paris Descartes, Paris F-75006; France 5 6 Running title: Trophoblast cell fusion 7 Key words: Human trophoblast, Cell fusion, Syncytins, Connexin 43, Cadherin, ZO-1, 8 cAMP-PKA signaling 9 10 Word count: 4276 11 12 13 †Corresponding author: Guillaume Pidoux, PhD 14 Inserm UMR-S-1139 15 Université Paris Descartes 16 Faculté de Pharmacie 17 Cell-Fusion group 18 75006 Paris, France 19 Tel: +33 1 53 73 96 02 20 Fax: +33 1 44 07 39 92 21 E-mail: [email protected] 22 1 23 Abstract 24 During human placentation, mononuclear cytotrophoblasts fuse to form a multinucleated syncytia 25 ensuring hormonal production and nutrient exchanges between the maternal and fetal circulation. -
Review Cell Fusion and Some Subcellular Properties Of
REVIEW CELL FUSION AND SOME SUBCELLULAR PROPERTIES OF HETEROKARYONS AND HYBRIDS SAIMON GORDON From the Genetics Laboratory, Department of Biochemistry, The University of Oxford, England, and The Rockefeller University, New York 10021 I. INTRODUCTION The technique of somatic cell fusion has made it first cell hybrids obtained by this method. The iso- possible to study cell biology in an unusual and lation of hybrid cells from such mixed cultures direct way. When cells are mixed in the presence of was greatly facilitated by the Szybalski et al. (6) Sendai virus, their membranes coalesce, the cyto- and Littlefield (7, 8) adaptation of a selective me- plasm becomes intermingled, and multinucleated dium containing hypoxanthine, aminopterin, and homo- and heterokaryons are formed by fusion of thymidine (HAT) to mammalian systems. similar or different cells, respectively (1, 2). By Further progress followed the use of Sendai fusing cells which contrast in some important virus by Harris and Watkins to increase the biologic property, it becomes possible to ask ques- frequency of heterokaryon formation (9). These tions about dominance of control processes, nu- authors exploited the observation by Okada that cleocytoplasmic interactions, and complementa- UV irradiation could be used to inactivate Sendai tion in somatic cell heterokaryons. The multinucle- virus without loss of fusion efficiency (10). It was ate cell may divide and give rise to mononuclear therefore possible to eliminate the problem of virus cells containing chromosomes from both parental replication in fused cells. Since many cells carry cells and become established as a hybrid cell line receptors for Sendai virus, including those of able to propagate indefinitely in vitro. -
Structural Insights Into Membrane Fusion Mediated by Convergent Small Fusogens
cells Review Structural Insights into Membrane Fusion Mediated by Convergent Small Fusogens Yiming Yang * and Nandini Nagarajan Margam Department of Microbiology and Immunology, Dalhousie University, Halifax, NS B3H 4R2, Canada; [email protected] * Correspondence: [email protected] Abstract: From lifeless viral particles to complex multicellular organisms, membrane fusion is inarguably the important fundamental biological phenomena. Sitting at the heart of membrane fusion are protein mediators known as fusogens. Despite the extensive functional and structural characterization of these proteins in recent years, scientists are still grappling with the fundamental mechanisms underlying membrane fusion. From an evolutionary perspective, fusogens follow divergent evolutionary principles in that they are functionally independent and do not share any sequence identity; however, they possess structural similarity, raising the possibility that membrane fusion is mediated by essential motifs ubiquitous to all. In this review, we particularly emphasize structural characteristics of small-molecular-weight fusogens in the hope of uncovering the most fundamental aspects mediating membrane–membrane interactions. By identifying and elucidating fusion-dependent functional domains, this review paves the way for future research exploring novel fusogens in health and disease. Keywords: fusogen; SNARE; FAST; atlastin; spanin; myomaker; myomerger; membrane fusion 1. Introduction Citation: Yang, Y.; Margam, N.N. Structural Insights into Membrane Membrane fusion -
BIO-210 Introduction to Biotechnology
Bergen Community College Division of Mathematics, Science, and Technology Department of Biology and Horticulture Introduction to Biotechnology (BIO-210) General Course Syllabus Spring 2016 Course Title: BIO-210 Introduction to Biotechnology Course Description: This course is designed to give students both a theoretical background and a working knowledge of the instrumentation and techniques employed in a biotechnology laboratory. Emphasis will be placed on the introduction of foreign DNA into bacterial cells, as well as the analysis of nucleic acids (DNA and RNA) and proteins. Prerequisites: BIO-101 General Biology I General Education Course: No Course Credits 4.0 Hours per week: 6.0: 3 hours lecture and 3 hours lab Course Coordinator: John Smalley Required Textbook: Introduction To Biotechnology, 3rd edition, Thieman, W.J. and M.A. Palladino. Pearson/Benjamin Cummings. Required Lab Manual: None Student Learning Objectives The student will be able to: 1. Students will demonstrate proper scientific laboratory record keeping. Students will be evaluated by periodic notebook collections. 2 Students will be able to explain the scientificbasis for each technique used. Students will be required to answer exam questions designed to allow them to demonstrate their acquisition and retention of this knowledge. 3. Students will learn how to introduce foreign DNA into bacterial cells for the purpose of molecular cloning. Students will be evaluated by observation in the laboratory and analysis of experimental results. Assessment will also be based upon performance on exam questions. 4. Students will be able to retrieve cloned DNA and analyze it using restriction endonuclease digestion and agarose gel electrophoresis. Students will be evaluated by observation in the laboratory and analysis of experimental results. -
HIGH SCHOOL COURSE OUTLINE (Revised June 2011)
OFFICE OF CURRICULUM, INSTRUCTION, & PROFESSIONAL DEVELOPMENT HIGH SCHOOL COURSE OUTLINE (Revised June 2011) Department Science Course Title Biotechnology 1-2 Course Code 3867 Abbreviation Biotech 1-2 Grade Level 10, 11 Grad Requirement No Credits per Approved Course Length 2 semesters 5 No Required No Elective Yes Semester for Honors Health Science and Biotechnology Research CTE Industry Sector CTE Pathway Medical Technology and Development Prerequisites Biology 1-2 with a "C" or better Co-requisites Integrated Math Program (IMP) 5-6 maintaining a “C” or better Articulated with LBCC No Articulated with CSULB No Meets UC “a-g” Requirement Yes (d) Meets NCAA Requirement Yes COURSE DESCRIPTION: Biotechnology 1-2 is a course designed to give students a comprehensive introduction to the scientific concepts and laboratory research techniques currently used in the field of biotechnology. Students attain knowledge about the field of biotechnology and deeper understanding of the biological concepts used. In addition, students develop the laboratory, critical thinking, and communication skills currently used in the biotechnology industry. Furthermore, students will explore and evaluate career opportunities in the field of biotechnology through extensive readings, laboratory experiments, class discussions, research projects, guest speakers, and workplace visits. The objectives covered in this course are both academic and technical in nature and are presented in a progressively rigorous manner. COURSE PURPOSE: GOALS (Student needs the course is intended to meet) Students will: CONTENT • Students will learn the basic biological and chemical processes of cell, tissues, and organisms. They will also learn the historical experiments that led to the central dogma of molecular biology and understand the basic processes of DNA replication, transcription and translation. -
Biology Major: Biotechnology Concentration (BIOL)—BS Degree
Biology Major: Biotechnology Concentration (BIOL)—B.S. Degree: Bachelor of Arts Required: 122 semester hours, to include at least 36 hours at or above the 300 course level AOS Code: U214 The concentration in biotechnology is designed for students with a strong interest in molecular biology and genetics. Courses will prepare students in both conceptual aspects of molecular biology and their practical application in biotechnology and genetic engineering. I General Education Core Requirements (GEC) See complete GEC requirements under General Education Program in the University Requirements section. See the GEC Course Summary Table for approved courses. GLT—Literature (6 s.h.) Student selects 6 s.h. from GLT list. GFA—Fine Arts (3.s.h.) Student selects 3 s.h. from GFA list. GPR—Philosophical, Religious, Ethical Principles (3 s.h.) Student selects 3 s.h. from GPR list. GHP—Historial Perspectives on Western Culture (3 s.h.) Student selects 3 s.h. from GHP list. GNS—Natural Sciences (7 s.h.) BIO 111 Principles of Biology I CHE 111 General Chemistry I GMT—Mathematics (3 s.h.) MAT 191 Calculus I GRD—Reasoning and Discourse (6 s.h.) ENG 101 College Writing I or FMS 115 Freshman Seminar in Reasoning and Discourse I or RCO 101 College Writing I Student selects additional 3 s.h. from the GRD list. GSB—Social and Behavioral Sciences (6 s.h.) Student selects 6 s.h. from GSB list. II General Education Marker Requirements See complete GEC requirements under General Education Program in the University Requirements section. See the GEC Course Summary Table for approved courses. -
Cell Fusion* Benjamin Podbilewicz1,2, §
Cell fusion* Benjamin Podbilewicz1,2, § 1 Department of Biology, Technion-Israel, Institute of Technology, Haifa 32000, Israel 2Section on Membrane Biology, Laboratory of Cellular and Molecular Biophysics, NICHD, NIH, Bethesda MD 20892, USA Table of Contents 1. Introduction ............................................................................................................................2 1.1. Ubiquitous cell fusion .................................................................................................... 2 1.2. Cell-to-cell fusion in worms ............................................................................................ 2 1.3. Humans and some nematodes have cellular skin but C. elegans has syncytia ............................. 3 2. Cell biology of plasma membrane fusion ...................................................................................... 5 3. Genetics of cell fusion .............................................................................................................. 7 4. eff-1 is necessary for most, but not all, cell fusion events in C. elegans ..............................................7 5. Regulation of cell fusion ........................................................................................................... 9 5.1. Transcriptional regulation of cell fusion ............................................................................. 9 5.2. Ventral cell fusions ..................................................................................................... -
CHAPTER 11 Lipid Acrobatics in the Membrane Fusion Arena
CHAPTER 11 Lipid Acrobatics in the Membrane Fusion Arena Albert J. Markvoort1 and Siewert J. Marrink2 1Institute for Complex Molecular Systems & Biomodeling and Bioinformatics Group, Eindhoven University of Technology, Eindhoven, The Netherlands 2Groningen Biomolecular Sciences and Biotechnology Institute & Zernike Institute for Advanced Materials, University of Groningen, Groningen, The Netherlands I. Overview II. Introduction III. Historical Background IV. Fusion Pathways at the Molecular Level A. Symmetric Stalk Expansion Pathway B. Alternative Pathways C. Composition Dependence V. Energy Landscape Along the Fusion Pathway A. Stalk and Hemifusion Diaphragm Intermediates B. Lipid Splaying as First Step C. Many Barriers to Cross VI. Fission Pathways in Molecular Detail A. Budding/Neck Formation B. Fission not Just Fusion Reversed VII. Peptide Modulated Fusion A. The Role of Fusion Peptides B. Protein-Induced Fusion VIII. Outlook References I. OVERVIEW In this review, we describe the recent contribution of computer simulation approaches to unravel the molecular details of membrane fusion. Over the past decade, fusion between apposed membranes and vesicles has been studied using a large variety of simulation methods and systems. Despite the variety in techniques, some generic fusion pathways emerge that predict a more complex Current Topics in Membranes, Volume 68 0065-230X/10 $35.00 Copyright 2011, Elsevier Inc. All right reserved. DOI: 10.1016/B978-0-12-385891-7.00011-8 260 Markvoort and Marrink picture beyond the traditional stalk–pore pathway. Indeed the traditional path- way is confirmed in particle-based simulations, but in addition alternative path- ways are observed in which stalks expand linearly rather than radially, leading to inverted-micellar or asymmetric hemifusion intermediates. -
Genetic Engineering (3500 Words)
Genetic Engineering (3500 words) Biology Also known as: biotechnology, gene splicing, recombinant DNA technology Anatomy or system affected: All Specialties and related fields: Alternative medicine, biochemistry, biotechnology, dermatology, embryology, ethics, forensic medicine, genetics, pharmacology, preventive medicine Definition: Genetic engineering, recombinant DNA technology and biotechnology – the buzz words you may have heard often on radio or TV, or read about in featured articles in newspapers or popular magazines. It is a set of techniques that are used to achieve one or more of three goals: to reveal the complex processes of how genes are inherited and expressed, to provide better understanding and effective treatment for various diseases, (particularly genetic disorders) and to generate economic benefits which include improved plants and animals for agriculture, and efficient production of valuable biopharmaceuticals. The characteristics of genetic engineering possess both vast promise and potential threat to human kind. It is an understatement to say that genetic engineering will revolutionize the medicine and agriculture in the 21st future. As this technology unleashes its power to impact our daily life, it will also bring challenges to our ethical system and religious beliefs. Key terms: GENETIC ENGINEERING: the collection of a wide array of techniques that alter the genetic constitution of cells or individuals by selective removal, insertion, or modification of individual genes or gene sets GENE CLONING: the development