micromachines Review Engineered Liver-On-A-Chip Platform to Mimic Liver Functions and Its Biomedical Applications: A Review 1, 1, 2, 1, 1 Jiu Deng y, Wenbo Wei y, Zongzheng Chen y , Bingcheng Lin *, Weijie Zhao , Yong Luo 1 and Xiuli Zhang 3,* 1 State Key Laboratory of Fine Chemicals, Department of Chemical Engineering, Dalian University of Technology, Dalian 116024, China;
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[email protected] (Y.L.) 2 Integrated Chinese and Western Medicine Postdoctoral research station, Jinan University, Guangzhou 510632, China;
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[email protected] (X.Z.); Tel.: +86-0411-8437-9065 (B.L.); +86-155-0425-7723 (X.Z.) These authors have equally contribution to this work. y Received: 17 September 2019; Accepted: 3 October 2019; Published: 7 October 2019 Abstract: Hepatology and drug development for liver diseases require in vitro liver models. Typical models include 2D planar primary hepatocytes, hepatocyte spheroids, hepatocyte organoids, and liver-on-a-chip. Liver-on-a-chip has emerged as the mainstream model for drug development because it recapitulates the liver microenvironment and has good assay robustness such as reproducibility. Liver-on-a-chip with human primary cells can potentially correlate clinical testing. Liver-on-a-chip can not only predict drug hepatotoxicity and drug metabolism, but also connect other artificial organs on the chip for a human-on-a-chip, which can reflect the overall effect of a drug.