Portland State University PDXScholar Dissertations and Theses Dissertations and Theses Summer 1-1-2012 The Role of Nucleotide Excision Repair in Restoring Replication Following UV-Induced Damage in Escherichia coli Kelley Nicole Newton Portland State University Follow this and additional works at: https://pdxscholar.library.pdx.edu/open_access_etds Part of the Biology Commons, and the Cell Biology Commons Let us know how access to this document benefits ou.y Recommended Citation Newton, Kelley Nicole, "The Role of Nucleotide Excision Repair in Restoring Replication Following UV- Induced Damage in Escherichia coli" (2012). Dissertations and Theses. Paper 767. https://doi.org/10.15760/etd.767 This Thesis is brought to you for free and open access. It has been accepted for inclusion in Dissertations and Theses by an authorized administrator of PDXScholar. Please contact us if we can make this document more accessible:
[email protected]. The Role of Nucleotide Excision Repair in Restoring Replication Following UV-Induced Damage in Escherichia coli by Kelley Nicole Newton A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Biology Thesis Committee: Justin Courcelle, Chair Michael Bartlett Jeffrey Singer Portland State University 2012 ABSTRACT Following low levels of UV exposure, Escherichia coli cells deficient in nucleotide excision repair recover and synthesize DNA at near wild type levels, an observation that formed the basis of the post replication recombination repair model. In this study, we characterized the DNA synthesis that occurs following UV-irradiation in the absence of nucleotide excision repair and show that although this synthesis resumes at near wild type levels, it is coincident with a high degree of cell death.