Magnesiumrich Intralensar Structures In
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[Palaeontology, Vol. 50, Part 5, 2007, pp. 1031–1037] MAGNESIUM-RICH INTRALENSAR STRUCTURES IN SCHIZOCHROAL TRILOBITE EYES by MARTIN R. LEE, CLARE TORNEY and ALAN W. OWEN Department of Geographical and Earth Sciences, University of Glasgow, Gregory Building, Lilybank Gardens, Glasgow G12 8QQ, UK; e-mail: [email protected] Typescript received 14 March 2007; accepted in revised form 25 May 2007 Abstract: The interpretation of the lenses of schizochroal ucts reflect original differences in mineral chemistry between trilobite eyes as aplanatic doublets by Clarkson and Levi-Setti the upper lens unit and lower intralensar bowl. The turbidity over 30 years ago has been widely accepted. However, the of the bowl and of the core within the upper part of the lens means of achieving a difference in refractive index across the are the result of their greater microporosity and abundance interface between the two parts of each lens to overcome of microdolomite inclusions, both of which were products of spherical aberration has remained a matter of speculation diagenetic replacement of original magnesian calcite in these and lately it has been argued that the doublet structure itself areas. Such a difference in magnesium concentration in the is no more than a diagenetic artefact. Recent advances in original calcite has long been postulated as one of the ways technologies for imaging, chemical analysis and crystallo- by which the interface between these lens units could have graphic characterization of minerals at high spatial resolu- produced an aberration-free image and the present study tions have enabled a re-examination of the structure of provides the first direct evidence of such a chemical contrast, calcite lenses at an unprecedented level of detail. The lenses thus confirming the doublet hypothesis. in the eyes of the specimen of Dalmanites sp. used in the ori- ginal formulation of the aplanatic doublet hypothesis are Key words: trilobite, schizochroal eyes, magnesian calcite, shown to have undergone diagenetic alteration, but its prod- microdolomite. The schizochroal eyes of the Lower Ordovician–Upper matter of speculation and, most recently, the doublet Devonian phacopine trilobites are unique amongst the structure itself has been interpreted as a diagenetic artefact Arthropoda (Horva´th et al. 1997). They are characterized (Bruton and Haas 2003). by a relatively small number of highly biconvex calcite Clarkson and Levi-Setti (1975, p. 665) speculated that lenses separated by cuticular material, the interlensar sclera the intralensar bowl was composed of calcite containing (see Clarkson et al. 2006 for review). The internal structure organic material, possibly chitin, whereas the upper unit of these lenses and the mechanism by which they focused was pure calcite. Horva´th (1989) argued that the lower light have been the subject of considerable debate. Drawing lens unit was wholly organic in composition whereas on their own observations and those of others (e.g. Towe Campbell (1975) and Miller and Clarkson (1980) sugges- 1973), Clarkson and Levi-Setti (1975) argued that each ted that differences in magnesium concentrations between lens was a doublet with the curved junction between the the two parts of the doublet might have provided the upper lens unit and the underlying bowl acting as a cor- necessary contrast in refractive index but they lacked the recting surface with a change in refractive index across it evidence to support this interpretation using the tech- bringing light into sharp focus. This elegant explanation niques then available. Nonetheless, such a difference in drew analogies between the upper lens units and the apla- magnesium content has been assumed to be the case (e.g. natic lenses for correcting spherical aberration postulated Fortey and Chatterton 2003). Magnesium enrichment has by the Renaissance scientists Descartes and Huygens (see also been invoked for an enigmatic structure termed the also Levi-Setti 1993). The doublet structure, seen in several core, reported in the upper lens units in some schizo- species in both transmitted light (e.g. Campbell 1975; Mil- chroal eyes (see Clarkson et al. 2006). ler and Clarkson 1980) and in etched surfaces by scanning More fundamentally, Bruton and Haas (2003) disputed electron microscopy (SEM) (Miller and Clarkson 1980), the doublet model and argued that the intralensar struc- and Clarkson and Levi-Setti’s explanation for it have tures described by Clarkson and Levi-Setti were diagenetic become widely accepted. However, the means of achieving artefacts. They proposed that focusing of light by lenses the necessary difference in refractive index across the inter- of the Devonian phacopine Geesops sparsinodosus was face of the two components of each lens has remained a achieved by grading the refractive index of lens calcite by ª The Palaeontological Association doi: 10.1111/j.1475-4983.2007.00710.x 1031 1032 PALAEONTOLOGY, VOLUME 50 an increase in organic material in the lateral parts of each quality map whereby contrast represents differences in the lens, thereby overcoming spherical aberration. quality of electron backscatter (Kikuchi) patterns which Recent technological advances in imaging and chemical reflect variations in crystallographic orientation of the and crystallographic analysis at high spatial resolutions calcite and the presence of subgrain boundaries and have enabled the structure of calcite lenses to be elucida- pores; the latter give very poor patterns. The precise ted at an unprecedented level of detail. The various hypo- orientation of the pole to a specified crystal plane for theses for lens structure and function outlined above can each point in the EBSD maps can also be plotted as a now be tested and here we report results of a re-examina- pole figure. Quantitative chemical analyses were acquired tion of the lenses of the specimen of an un-named species using a Cameca SX50 electron probe operated at of Dalmanites (horizon and locality not known) that 15 kV ⁄ 10 nA and with a 10-lm defocused spot. Stan- formed a crucial part of the doublet hypothesis of Clark- dardization used wollastonite (Ca), periclase (Mg), Mn son and Levi-Setti (1975; also Horva´th 1989; Levi-Setti metal (Mn) and Fe metal (Fe). Count times were typically 1993). If the lenses were doublets, the two parts of each 30 s on peak and 10 s on background, and detection lens must have differed significantly in refractive index limits were 0Æ06 weight per cent MnCO3 and 0Æ07 weight and therefore in composition. The new technologies for per cent FeCO3. mineral characterization now provide an opportunity to assess whether such compositional differences did origin- ally exist at the described interface. The absence of a con- RESULTS trast between the upper lens unit and bowl sufficient to yield the necessary difference in refractive index (e.g. cal- The trilobite exoskeleton studied is contained in a skeletal cite vs. chitin, magnesium-poor calcite vs. magnesium- packstone together with articulated and disarticulated rich calcite) would lend support to the contention by microfossils, including ostracodes, and fragments of trilo- Bruton and Haas that the doublet structure is a diagenetic bites and echinoderms. Small angular grains of quartz artefact, or at the very least that all of the original differ- also occur. The limestone is orange in optical-CL and has ences within the structure have been completely overprin- a greater luminescence intensity than the trilobite cuticles. ted by recrystallization during burial. In plane polarized transmitted light the lenses are defined clearly and have abrupt boundaries with the interlensar sclera and limestone (Text-fig. 1). Many of the lenses METHODS contain a bowl and core, both of which are turbid and pseudopleochroic in plane polarized transmitted light and The internal structure of the lenses and adjacent exoskel- so are distinguished clearly from the enclosing optically eton on polished thin sections of the indeterminate spe- clear lens calcite (Text-fig. 1). A small proportion of the cies of Dalmanites was observed initially by transmitted lenses are turbid throughout, although the bowl can still light and optical cathodoluminescence (optical-CL) micro- be recognized by a greater opacity. BSE imaging shows scopy. Higher resolution imaging and chemical and crys- that the turbidity of the bowl and core is due mainly to tallographic analysis of the lenses used an FEI Quanta 200F field-emission environmental scanning electron microscope equipped with an EDAX ⁄ TSL X-ray micro- analysis and electron backscatter diffraction (EBSD) sys- tem. Conventional backscattered electron (BSE) imaging of lenses in thin section was used in conjunction with a new technique of charge contrast (CC) microscopy that utilizes secondary electrons emitted from uncoated samples with the microscope operated in environmental mode. Contrast within CC images reflects variations in the accumulation and dissipation of electrons on the sample surface (Watt et al. 2000) and may be comparable with that formed by optical-CL, but the CC images can be acquired at much higher magnifications and from non-luminescent minerals (Cuthbert and Buckman 2005). EBSD was used to determine the crystallographic orien- TEXT-FIG. 1. Plane polarized transmitted light image of a tation of lens calcite, and sample preparation and instru- single lens. Faint trabeculae that fan out downwards can be ment operating conditions are described in Dalbeck et al. identified within the core. The subhorizontal black line at the (2006). Here the EBSD data are presented as an image top of the lens is a fracture. LEE ET AL.: INTRALENSAR STRUCTURES IN SCHIZOCHROAL TRILOBITE EYES 1033 abundant micropores (Text-fig. 2A). The core has a in central and outer parts of the lenses, but fan outwards microporosity of c. 1Æ2 vol. per cent (determined from towards the base of the lens where they are orientated at computer analysis of BSE images) and the pores range in angles of up to 40 degrees to the c-axis (Text-fig. 3A).