Apibacter Adventoris Gen. Nov., Sp. Nov., a Member of the Phylum Bacteroidetes Isolated from Honey Bees Waldan K

Total Page:16

File Type:pdf, Size:1020Kb

Apibacter Adventoris Gen. Nov., Sp. Nov., a Member of the Phylum Bacteroidetes Isolated from Honey Bees Waldan K International Journal of Systematic and Evolutionary Microbiology (2016), 66, 1323–1329 DOI 10.1099/ijsem.0.000882 Apibacter adventoris gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from honey bees Waldan K. Kwong1,2 and Nancy A. Moran2 Correspondence 1Department of Ecology and Evolutionary Biology, Yale University, New Haven, CT, USA Waldan K. Kwong 2Department of Integrative Biology, University of Texas, Austin, TX, USA [email protected] Honey bees and bumble bees harbour a small, defined set of gut bacterial associates. Strains matching sequences from 16S rRNA gene surveys of bee gut microbiotas were isolated from two honey bee species from East Asia. These isolates were mesophlic, non-pigmented, catalase-positive and oxidase-negative. The major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH, C16 : 0 and C16 : 0 3-OH. The DNA G+C content was 29–31 mol%. They had ,87 % 16S rRNA gene sequence identity to the closest relatives described. Phylogenetic reconstruction using 20 protein-coding genes showed that these bee-derived strains formed a highly supported monophyletic clade, sister to the clade containing species of the genera Chryseobacterium and Elizabethkingia within the family Flavobacteriaceae of the phylum Bacteroidetes. On the basis of phenotypic and genotypic characteristics, we propose placing these strains in a novel genus and species: Apibacter adventoris gen. nov., sp. nov. The type strain of Apibacter adventoris is wkB301T (5NRRL B-65307T5NCIMB 14986T). Bacteria from the phylum Bacteroidetes are often constitu- In July and August of 2014, samples of the Asian honey bee ents of animal microbiotas. In humans, members of the (Apis cerana) and the giant honey bee (Apis dorsata) were genera Prevotella and Bacteroides are among the most collected from Singapore and Kuala Lumpur, Malaysia numerically abundant gut symbionts, and appear to (Table S1, available in the online Supplementary Material). anchor different states of stability (enterotypes) within The entire intestinal tract was removed and crushed in the community (Arumugam et al. 2011). Honey bees and 19 % (v/v) glycerol and frozen. Bacteria were recovered bumble bees have also been found to harbour members by plating out the frozen samples onto heart infusion of the phylum Bacteroidetes: culture-independent 16S agar (Hardy Diagnostics) supplemented with 5 % defibri- rRNA gene surveys of their gut communities have ident- nated sheep blood and incubating at 35 8Cin5%CO2. ified sequences falling within the order Flavobacteriales, After 2–3 days, small (,0.5 mm) white, non-haemolytic with .10 % divergence from described species. Although colonies appeared. Three isolates were chosen for further their prevalence appears sporadic compared with the core characterization: wkB180, wkB309 and wkB301T. bee gut microbiota (Babendreier et al., 2007; Koch & Strains wkB180, wkB309 and wkB301T grew on heart infusion Schmid-Hempel, 2011; Ahn et al., 2012; Moran et al., agar, brain heart infusion agar, trypticase soy agar, Columbia 2012; Lim et al., 2015), the bee Flavobacteriales sequences agar and lysogeny broth (LB) agar, but exhibited poor or no form a monophyletic clade, suggesting they are specific to growth on nutrient agar, MacConkey agar, Lactobacillus bee guts, or to other environments (e.g. plants, nectar, MRS agar or R2A agar (all media from BD Difco). No hive material) where they are prone to be ingested by growth was observed under anaerobic conditions (90 % N , bees. Here, we provide the first description of members 2 5% CO,5%H) or in ambient air. In liquid culture, of this novel group. 2 2 growth was observed in Insectagro DS2 (Corning), Columbia broth, trypticase soy broth, and LB (without NaCl), but not in Lactobacillus MRS. On solid media, the strains formed The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA smooth, round, pale/semi-translucent colonies. Strain T gene sequences of strains wkB301 , wkB309 and wkB180 are wkB309 exhibited rapidly spreading margins of growth KT149221, KT149220 and KT149222 respectively. Protein-coding extending from colony edges, consistent with gliding motility. genes from these strains are deposited under the accession numbers KT149223– KT149282. DNA was extracted as described by Powell et al. (2014) Four supplementary tables and one supplementary figure are available with the following modifications. RNase A was added with the online Supplementary Material. directly to cetyltrimethylammonium bromide (CTAB) Downloaded from www.microbiologyresearch.org by 000882 G 2016 IUMS Printed in Great Britain 1323 IP: 54.70.40.11 On: Sun, 04 Nov 2018 04:06:39 W. K. Kwong and N. A. Moran buffer prior to bead-beating, a final concentration of 0.5 % Ornithobacterium rhinotracheale, Chryseobacterium gleum 2-mercaptoethanol was used, and the lysate was incubated and Flavobacterium aquatile, respectively (Table S1). How- at 56 8C for 1 h instead of overnight. Samples were sub- ever, our strains could not be confidently placed in relation mitted to the Genome Sequencing and Analysis Facility to the clades encompassing the genera Empedobacter and at the University of Texas at Austin for Illumina MiSeq Chryseobacterium (clades E and C, respectively) using 16S 26300 bp library preparation and whole genome sequen- rRNA gene sequences alone (Fig. 1). To obtain better cing. Sequence reads were assembled with CLC Genomics phylogenetic resolution, we extracted and aligned 20 pro- Workbench 5.5 (CLC bio). Genome sizes and DNA tein sequences from the strains’ genomes and that of G+C content were inferred from draft assemblies. sequenced relatives to produce a tree based on 9746 total residues (Fig. 2). This tree strongly suggested that the bee Phylogenetic analysis based on 16S rRNA sequences gut strains are sister to clade C, and not to clade E. All phy- (1250 bp) showed that strains wkB180, wkB309 and logenetic analysis was done in MEGA 6 (Tamura et al. 2013). wkB301T clustered together and were only distantly related to previously described species (Fig. 1). Strain wkB301T We conducted further assays to characterize the phenotypic had 87.6, 87.5, 87.2 and 85.5 % 16S rRNA gene sequence traits of our strains. Scanning electron micrographs identity to the type strains of Empedobacter brevis, were taken to determine cell size and morphology. LMG 18212T (AJ271010) 97 Chryseobacterium joosteii T 61 Chryseobacterium ureilyticum F-Fue-04IIIaaaa (AM232806) 92 T Chryseobacterium vrystaatense R-23566 (AJ871397) T 39 Chryseobacterium gleum ATCC 35910 (ACKQ01000057) T 0.2 Chryseobacterium hominis NF802 (AM261868) 100 H9T (EF204446) 22 Chryseobacterium bovis T 100 Chryseobacterium daecheongense CPW406 (AJ457206) T Chryseobacterium wanjuense R2A10-2 (DQ256729) 39 LMG 23089T (AM040439) 96 Chryseobacterium piscium 50 T Chryseobacterium indoltheticum LMG 4025 (AY468448) 51 T Chryseobacterium aquaticum 10-46 (AM748690) T Clade C 71 Chryseobacterium xinjiangense TSBY 67 (DQ166169) T 69 100 Epilithonimonas tenax EP105 (AF493696) T Epilithonimonas lactis H1 (NR_115989) T 100 Cloacibacterium normanense CCUG 46293 (AJ575430) T Cloacibacterium rupense R2A-16 (EU581834) T 77 88 Bergeyella zoohelcum ATCC 43767 (AGYA01000006) T 78 Riemerella anatipestifer DSM 15868 (NR_074429) Flavobacteriaceae 90 8151T (NR_117970) Riemerella columbipharyngis Bacteroidetes 100 T Soonwooa buanensis HM0024 (NR_116776) 51 T Elizabethkingia meningoseptica ATCC 13253 (ASAN01000081) 100 T 47 Elizabethkingia anophelis R26 (NR_116021) T Cruoricaptor ignavus IMMIB L-12475 (NR_108875) T Ornithobacterium rhinotracheale DSM 15997 (CP003283) 40 T Moheibacter sediminis M0116 (KF694750) T Weeksella virosa DSM 16922 (CP002455) 80 85 T (KF694751) 51 100 Chishuiella changwenlii BY4 Clade E T 88 Empedobacter falsenii NF 993 (AM084341) T Empedobacter brevis LMG 4011 (AM177497) 100 Apibacter adventoris wkB180 (KT149222) Apis dorsata Apibacter adventoris wkB301T (KT149221) 100 100 uncultured clone HBG A2V3-2 (DQ837638) Apis mellifera uncultured clone HBG A2V3-4 (DQ837639) 100 sp. B1 (KJ741206) 100 Bacteroidetes 100 Apis cerana Bee-associated Apibacter adventoris wkB309 (KT149220) 85 uncultured clone BJ07179A4 (HM215037) 100 uncultured clone D08062F1 (HM215038) Bumble bee uncultured clone D08033D2 (HM215036) 97 uncultured clone Bt231.55A2 (JQ388908) Flavobacterium aquatile LMG 4008T (JRHH01000003) Prevotella melaninogenica ATCC 25845T (AY323525) Bacteroides fragilis NCTC 9343T (CR626927) Escherichia coli MG1655 (NC_000913) Fig. 1. 16S rRNA gene phylogeny of strains wkB301T, wkB309, wkB180 and close relatives. Hosts of origin for bee-derived sequences or strains are highlighted. The tree was built with a maximum-likelihood algorithm using the Generalized Time Reversible substitution model with gamma-distributed rates and invariant sites. Escherichia coli MG1655 is the outgroup. Bootstrap percentages (based on 1000 replicates) are shown at nodes. Bar, 0.2 substitutions per site. Downloaded from www.microbiologyresearch.org by 1324 International Journal of Systematic and Evolutionary Microbiology 66 IP: 54.70.40.11 On: Sun, 04 Nov 2018 04:06:39 Apibacter adventoris gen. nov., sp. nov. T 100 Chryseobacterium indologenes NBRC 14944 (NZ_BAVL00000000) 99 T 0.2 Chryseobacterium gleum ATCC 35910 (NZ_ACKQ00000000) 100 Chryseobacterium taiwanense TPW19 (NZ_JWTA00000000) 99 Chryseobacterium vrystaatense LMG 22846T (NZ_JPRI00000000) 100 Epilithonimonas lactis H1T (NZ_JPLY00000000) Chryseobacterium haifense DSM 19056T (NZ_JASZ00000000) 73 100 Chryseobacterium antarcticum LMB 24720 (NZ_JPEP00000000) Clade
Recommended publications
  • The Expanded Database of Polysaccharide Utilization Loci Nicolas Terrapon1,2,*, Vincent Lombard1,2, Elodie´ Drula1,2, Pascal Lapebie´ 1,2, Saad Al-Masaudi3, Harry J
    Published online 27 October 2017 Nucleic Acids Research, 2018, Vol. 46, Database issue D677–D683 doi: 10.1093/nar/gkx1022 PULDB: the expanded database of Polysaccharide Utilization Loci Nicolas Terrapon1,2,*, Vincent Lombard1,2, Elodie´ Drula1,2, Pascal Lapebie´ 1,2, Saad Al-Masaudi3, Harry J. Gilbert4 and Bernard Henrissat1,2,3,* 1Architecture et Fonction des Macromolecules´ Biologiques, CNRS, Aix-Marseille Universite,´ F-13288 Marseille, France, 2USC1408 Architecture et Fonction des Macromolecules´ Biologiques, Institut National de la Recherche Agronomique, F-13288 Marseille, France, 3Department of Biological Sciences, King Abdulaziz University, 23218 Jeddah, Saudi Arabia and 4Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne NE2 4HH, UK Received September 17, 2017; Revised October 16, 2017; Editorial Decision October 17, 2017; Accepted October 25, 2017 ABSTRACT INTRODUCTION The Polysaccharide Utilization Loci (PUL) database Polysaccharides constitute the main source of carbon for was launched in 2015 to present PUL predictions most organisms on Earth. Because of their enormous struc- in ∼70 Bacteroidetes species isolated from the hu- tural diversity, polysaccharide deconstruction requires the man gastrointestinal tract, as well as PULs derived concerted action of large numbers of specific enzymes. from the experimental data reported in the litera- While most bacteria break down polysaccharides by export- ing their carbohydrate-active enzymes (CAZymes) into the ture. In 2018 PULDB offers access to 820 genomes, extracellular milieu and import the simple sugars produced, sampled from various environments and covering a an inventive solution operates in Gram-negative bacteria of much wider taxonomical range. A Krona dynamic the Bacteroidetes phylum. The genomes of these bacteria chart was set up to facilitate browsing through tax- feature Polysaccharide Utilization Loci, or PULs.
    [Show full text]
  • Spatiotemporal Dynamics of Marine Bacterial and Archaeal Communities in Surface Waters Off the Northern Antarctic Peninsula
    Spatiotemporal dynamics of marine bacterial and archaeal communities in surface waters off the northern Antarctic Peninsula Camila N. Signori, Vivian H. Pellizari, Alex Enrich Prast and Stefan M. Sievert The self-archived postprint version of this journal article is available at Linköping University Institutional Repository (DiVA): http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-149885 N.B.: When citing this work, cite the original publication. Signori, C. N., Pellizari, V. H., Enrich Prast, A., Sievert, S. M., (2018), Spatiotemporal dynamics of marine bacterial and archaeal communities in surface waters off the northern Antarctic Peninsula, Deep-sea research. Part II, Topical studies in oceanography, 149, 150-160. https://doi.org/10.1016/j.dsr2.2017.12.017 Original publication available at: https://doi.org/10.1016/j.dsr2.2017.12.017 Copyright: Elsevier http://www.elsevier.com/ Spatiotemporal dynamics of marine bacterial and archaeal communities in surface waters off the northern Antarctic Peninsula Camila N. Signori1*, Vivian H. Pellizari1, Alex Enrich-Prast2,3, Stefan M. Sievert4* 1 Departamento de Oceanografia Biológica, Instituto Oceanográfico, Universidade de São Paulo (USP). Praça do Oceanográfico, 191. CEP: 05508-900 São Paulo, SP, Brazil. 2 Department of Thematic Studies - Environmental Change, Linköping University. 581 83 Linköping, Sweden 3 Departamento de Botânica, Instituto de Biologia, Universidade Federal do Rio de Janeiro (UFRJ). Av. Carlos Chagas Filho, 373. CEP: 21941-902. Rio de Janeiro, Brazil 4 Biology Department, Woods Hole Oceanographic Institution (WHOI). 266 Woods Hole Road, Woods Hole, MA 02543, United States. *Corresponding authors: Camila Negrão Signori Address: Departamento de Oceanografia Biológica, Instituto Oceanográfico, Universidade de São Paulo, São Paulo, Brazil.
    [Show full text]
  • The Prevalence of Foodborne Pathogenic Bacteria on Cutting Boards and Their Ecological Correlation with Background Biota
    AIMS Microbiology, 2(2): 138-151. DOI: 10.3934/microbiol.2016.2.138 Received: 23 April 2016 Accepted: 19 May 2016 Published: 22 May 2016 http://www.aimspress.com/journal/microbiology Research article The prevalence of foodborne pathogenic bacteria on cutting boards and their ecological correlation with background biota Noor-Azira Abdul-Mutalib 1,2,3, Syafinaz Amin Nordin 2, Malina Osman 2, Ahmad Muhaimin Roslan 4, Natsumi Ishida 5, Kenji Sakai 5, Yukihiro Tashiro 5, Kosuke Tashiro 6, Toshinari Maeda 1, and Yoshihito Shirai 1,* 1 Department of Biological Functions and Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu-ku, Kitakyushu, Fukuoka 808-0196, Japan 2 Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia 3 Department of Food Service and Management, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia 4 Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia 5 Laboratory of Soil Microbiology, Faculty of Agriculture, Graduate School, Kyushu University, 6- 10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan 6 Laboratory of Molecular Gene Technique, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan * Correspondence: E-mail: [email protected]; Tel.: +6012-9196951; Fax: +603-89471182. Abstract: This study implemented the pyrosequencing technique and real-time quantitative PCR to determine the prevalence of foodborne pathogenic bacteria (FPB) and as well as the ecological correlations of background biota and FPB present on restaurant cutting boards (CBs) collected in Seri Kembangan, Malaysia.
    [Show full text]
  • High Quality Permanent Draft Genome Sequence of Chryseobacterium Bovis DSM 19482T, Isolated from Raw Cow Milk
    Lawrence Berkeley National Laboratory Recent Work Title High quality permanent draft genome sequence of Chryseobacterium bovis DSM 19482T, isolated from raw cow milk. Permalink https://escholarship.org/uc/item/4b48v7v8 Journal Standards in genomic sciences, 12(1) ISSN 1944-3277 Authors Laviad-Shitrit, Sivan Göker, Markus Huntemann, Marcel et al. Publication Date 2017 DOI 10.1186/s40793-017-0242-6 Peer reviewed eScholarship.org Powered by the California Digital Library University of California Laviad-Shitrit et al. Standards in Genomic Sciences (2017) 12:31 DOI 10.1186/s40793-017-0242-6 SHORT GENOME REPORT Open Access High quality permanent draft genome sequence of Chryseobacterium bovis DSM 19482T, isolated from raw cow milk Sivan Laviad-Shitrit1, Markus Göker2, Marcel Huntemann3, Alicia Clum3, Manoj Pillay3, Krishnaveni Palaniappan3, Neha Varghese3, Natalia Mikhailova3, Dimitrios Stamatis3, T. B. K. Reddy3, Chris Daum3, Nicole Shapiro3, Victor Markowitz3, Natalia Ivanova3, Tanja Woyke3, Hans-Peter Klenk4, Nikos C. Kyrpides3 and Malka Halpern1,5* Abstract Chryseobacterium bovis DSM 19482T (Hantsis-Zacharov et al., Int J Syst Evol Microbiol 58:1024-1028, 2008) is a Gram-negative, rod shaped, non-motile, facultative anaerobe, chemoorganotroph bacterium. C. bovis is a member of the Flavobacteriaceae, a family within the phylum Bacteroidetes. It was isolated when psychrotolerant bacterial communities in raw milk and their proteolytic and lipolytic traits were studied. Here we describe the features of this organism, together with the draft genome sequence and annotation. The DNA G + C content is 38.19%. The chromosome length is 3,346,045 bp. It encodes 3236 proteins and 105 RNA genes. The C. bovis genome is part of the Genomic Encyclopedia of Type Strains, Phase I: the one thousand microbial genomes study.
    [Show full text]
  • Bacterial Microbiome of the Nose of Healthy Dogs and Dogs with Nasal Disease
    RESEARCH ARTICLE Bacterial microbiome of the nose of healthy dogs and dogs with nasal disease Barbara Tress1, Elisabeth S. Dorn1, Jan S. Suchodolski2, Tariq Nisar2, Prajesh Ravindran2, Karin Weber1, Katrin Hartmann1, Bianka S. Schulz1* 1 Clinic of Small Animal Medicine, LMU Munich, Munich, Germany, 2 Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas, United States of America * [email protected] Abstract a1111111111 The role of bacterial communities in canine nasal disease has not been studied so far a1111111111 a1111111111 using next generation sequencing methods. Sequencing of bacterial 16S rRNA genes has a1111111111 revealed that the canine upper respiratory tract harbors a diverse microbial community; a1111111111 however, changes in the composition of nasal bacterial communities in dogs with nasal dis- ease have not been described so far. Aim of the study was to characterize the nasal micro- biome of healthy dogs and compare it to that of dogs with histologically confirmed nasal neoplasia and chronic rhinitis. Nasal swabs were collected from healthy dogs (n = 23), dogs OPEN ACCESS with malignant nasal neoplasia (n = 16), and dogs with chronic rhinitis (n = 8). Bacterial DNA was extracted and sequencing of the bacterial 16S rRNA gene was performed. Data were Citation: Tress B, Dorn ES, Suchodolski JS, Nisar T, Ravindran P, Weber K, et al. (2017) Bacterial analyzed using Quantitative Insights Into Microbial Ecology (QIIME). A total of 376 Opera- microbiome of the nose of healthy dogs and dogs tional Taxonomic Units out of 26 bacterial phyla were detected.
    [Show full text]
  • Bacterial Profiles and Antibiogrants of the Bacteria Isolated of the Exposed Pulps of Dog and Cheetah Canine Teeth
    Bacterial profiles and antibiogrants of the bacteria isolated of the exposed pulps of dog and cheetah canine teeth A dissertation submitted to the Faculty of Veterinary Science, University of Pretoria. In partial fulfillment of the requirements for the degree Master of Science (Veterinary Science) Promoter: Dr. Gerhard Steenkamp Co-promoter: Ms. Anna-Mari Bosman Department of Companion Animal Clinical Studies Faculty of Veterinary Science University of Pretoria Pretoria (January 2012) J.C. Almansa Ruiz © University of Pretoria Declaration I declare that the dissertation that I hereby submit for the Masters of Science degree in Veterinary Science at the University of Pretoria has not previously been submitted by me for degree purposes at any other university. J.C. Almansa Ruiz ii Dedications To one of the most amazing hunters of the African bush, the Cheetah, that has made me dream since I was a child, and the closest I had been to one, before starting this project was in National Geographic documentaries. I wish all of them a better future in which their habitat will be more respected. To all conservationists, especially to Carla Conradie and Dave Houghton, for spending their lives saving these animals which are suffering from the consequences of the encroachment of human beings into their territory. Some, such as George Adamson, the lion conservationist, even lost their lives in this mission. To the conservationist, Lawrence Anthony, for risking his life in a suicide mission to save the animals in the Baghdad Zoo, when the conflict in Iraq exploded. To my mother and father Jose Maria and Rosa.
    [Show full text]
  • Das Mikrobiom Periimplantärer Läsionen Der Nachweis Dysbiotischer Veränderungen in Assoziation Mit Dem Schweregrad Der Erkrankung
    Das Mikrobiom periimplantärer Läsionen Der Nachweis dysbiotischer Veränderungen in Assoziation mit dem Schweregrad der Erkrankung Inaugural-Dissertation zur Erlangung des Doktorgrades der Hohen Medizinischen Fakultät der Rheinischen Friedrich-Wilhelms-Universität Bonn Annika Therese Kröger aus Aachen 2020 Angefertigt mit der Genehmigung der Medizinischen Fakultät der Universität Bonn 1. Gutachter: Prof. Dr. med. dent. Moritz Kebschull 2. Gutachter: Prof. Dr. Achim Hörauf Tag der Mündlichen Prüfung: 07.10.2020 Aus der Poliklinik für Parodontologie, Zahnerhaltung und Präventive Zahnheilkunde Direktor: Prof. Dr. med. Dr. med. dent. Søren Jepsen Meinen Eltern 5 Inhaltsverzeichnis Abkürzungsverzeichnis 7 1. Einleitung 8 1.1 Periimplantitis 8 1.1.1 Dentale Implantate und umgebendes Gewebe in periimplantären gesunden Situationen 8 1.1.2 Periimplantitis 10 1.1.3 Periimplantitis versus Parodontitis 14 1.2 Das Mikrobiom bei Periimplantitis und Parodontitis 17 1.3 16s rRNA Sequenzierung 18 1.3.1 Hochdurchsatzsequenziermethodiken 18 1.3.2 Das 16s Gen 18 1.4 Hypothese und Fragestellung dieser Studie 19 2. Material und Methoden 21 2.1 Studienpopulation 21 2.1.1 Allgemeines 21 2.1.2 Ein- und Ausschlusskriterien 21 2.1.3 Dokumentierte Parameter 22 2.2 Probengewinnung und -aufbereitung 23 2.3 16s rRNA Sequenzierung und Datenaufbereitung 24 2.3.1 ‚Paired-End‘-Hochdurchsatz-Sequenzierung der V3-V4 Region des 16s rRNA Genes 25 2.3.2 Post-Sequenzierungs-Prozesse 28 2.4 Statistische Analyse 32 2.4.1 Assoziation mit PD 32 2.4.2 Netzwerkanalyse 33 2.4.3 Mikrobieller Dysbiose Index 34 2.4.4 Signifikante Unterschiede der bakteriellen Charakteristika 34 6 3. Ergebnisse 35 3.1 Studienpopulation (Tab.
    [Show full text]
  • University of Veterinary Medicine Hannover
    University of Veterinary Medicine Hannover Investigations on the taxonomy of the genus Riemerella and diagnosis of Riemerella infections in domestic poultry and pigeons Thesis Submitted in partial fulfilment of the requirements for the degree - Doctor of Veterinary Medicine - Doctor medicinae veterinariae (Dr. med. vet.) by Dennis Rubbenstroth, PhD Bielefeld Hannover 2012 Academic supervision Prof. S. Rautenschlein (Clinic for Poultry, University of Veterinary Medicine Hannover, Germany) 1st Referee Prof. S. Rautenschlein 2nd Referee Prof. P. Valentin-Weigand (Institute of Microbiology, University of Veterinary Medicine Hannover, Germany) Date of oral exam: November 7 th , 2012 Meinen beiden Großmüttern in dankbarer Erinnerung Table of contents v Table of contents Table of contents....................................................................................................................... v List of abbreviations ...............................................................................................................vii Manuscripts and participation of this author ........................................................................viii 1. Introduction .................................................................................................................. 1 2. Literature review .......................................................................................................... 3 2.1. Taxonomy of the genus Riemerella ...................................................................... 3 2.2. Morphology,
    [Show full text]
  • University of Oklahoma Graduate College
    UNIVERSITY OF OKLAHOMA GRADUATE COLLEGE MICROBIOLOGY OF WATER AND WASTEWATER: DISCOVERY OF A NEW GENUS NUMERICALLY DOMINANT IN MUNICIPAL WASTEWATER AND ANTIMICROBIAL RESISTANCES IN NUMERICALLY DOMINANT BACTERIA FROM OKLAHOMA LAKES A DISSERTATION SUBMITTED TO THE GRADUATE FACULTY in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY By Toby D. Allen Norman, Oklahoma 2005 UMI Number: 3203299 UMI Microform 3203299 Copyright 2006 by ProQuest Information and Learning Company. All rights reserved. This microform edition is protected against unauthorized copying under Title 17, United States Code. ProQuest Information and Learning Company 300 North Zeeb Road P.O. Box 1346 Ann Arbor, MI 48106-1346 MICROBIOLOGY OF WATER AND WASTEWATER: DISCOVERY OF A NEW GENUS NUMERICALLY DOMINANT IN MUNICIPAL WASTEWATER AND ANTIMICROBIAL RESISTANCES IN NUMERICALLY DOMINANT BACTERIA FROM OKLAHOMA LAKES A DISSERTATION APPROVED FOR THE DEPARTMENT OF BOTANY AND MICROBIOLOGY BY ____________________________ Dr. Ralph S. Tanner ____________________________ Dr. Kathleen E. Duncan ____________________________ Dr. David P. Nagle ____________________________ Dr. Mark A. Nanny ____________________________ Dr. Marvin Whiteley Copyright by Toby D. Allen 2005 All Rights Reserved “Science advances through tentative answers to a series of more and more subtle questions which reach deeper and deeper into the essence of natural phenomena” – Louis Pasteur iv ACKNOWLEDGEMENTS I consider myself fortunate to have had the opportunity to work on the projects contained in this work. I am grateful to have had the support and guidance of Dr. Ralph Tanner, who gave me the opportunity conduct research in his laboratory and to the Department of Botany and Microbiology, which has supported me in the form of teaching and research assistantships.
    [Show full text]
  • | Hao Watatu Timur Alma Mult
    |HAO WATATU US010058101B2TIMUR ALMA MULT (12 ) United States Patent ( 10 ) Patent No. : US 10 ,058 , 101 B2 von Maltzahn et al. (45 ) Date of Patent: * Aug. 28 , 2018 ( 54 ) METHODS OF USE OF SEED -ORIGIN ( 52 ) U . S . CI. ENDOPHYTE POPULATIONS CPC . .. .. .. AOIN 63 /00 (2013 .01 ) ; A01C 1 / 06 ( 2013 .01 ) ; A01N 63 / 02 ( 2013 .01 ) ; GOIN (71 ) Applicant: Indigo Ag , Inc. , Boston , MA (US ) 33/ 5097 ( 2013 . 01 ) ; A01C 1 / 60 ( 2013. 01 ) ( 72 ) Inventors : Geoffrey von Maltzahn , Boston , MA (58 ) Field of Classification Search (US ) ; Richard Bailey Flavell , None Thousand Oaks , CA (US ) ; Gerardo V . See application file for complete search history . Toledo , Belmont, MA (US ) ; Slavica Djonovic , Malden , MA (US ) ; Luis Miguel Marquez , Belmont, MA ( US) ; (56 ) References Cited David Morris Johnston , Cambridge , MA (US ) ; Yves Alain Millet, U . S . PATENT DOCUMENTS Newtonville , MA (US ) ; Jeffrey Lyford , 4 , 940 , 834 A 7 / 1990 Hurley et al . Hollis , NH (US ) ; Alexander Naydich , 5 ,041 , 290 A 8 / 1991 Gindrat et al. Cambridge , MA (US ) ; Craig Sadowski, 5 , 113 ,619 A 5 / 1992 Leps et al . 5 , 229 , 291 A * 7 / 1993 Nielsen . .. .. .. .. C12N 15 /00 Somerville , MA (US ) 435 / 244 5 , 292, 507 A 3 / 1994 Charley ( 73 ) Assignee : Indigo Agriculture, Inc. , Boston , MA 5 ,415 ,672 A 5 / 1995 Fahey et al . (US ) 5 ,730 , 973 A 3 / 1998 Morales et al. 5 , 994 , 117 A 11/ 1999 Bacon et al . 6 ,072 , 107 A 6 / 2000 Latch et al. ( * ) Notice : Subject to any disclaimer, the term of this 6 , 077 , 505 A 6 / 2000 Parke et al.
    [Show full text]
  • Multilocus Sequence Typing of the Guangdong Isolates of Riemerella Anatipestifer from Ducks in China
    Open Journal of Animal Sciences, 2015, 5, 332-342 Published Online July 2015 in SciRes. http://www.scirp.org/journal/ojas http://dx.doi.org/10.4236/ojas.2015.53037 Multilocus Sequence Typing of the Guangdong Isolates of Riemerella anatipestifer from Ducks in China B. F. Zhu1,2, Mike Chao3*, X. F. Yang1,2, D. Zhou4 1Henry Fok College of Life Science, Shaoguan University, Shaoguan, China 2Joint Laboratory of Animal Infectious, Diseases Diagnostic Center, Harbin Veterinary Research Institute, CAAS-Shaoguan University, Shaoguan, China 3Department of Biology Science of College of Nature Science, California State University, San Bernardino, USA 4College of Life Science and Foof Engineering, Nanchang University, Nanchang, China Email: *[email protected] Received 22 April 2015; accepted 19 July 2015; published 22 July 2015 Copyright © 2015 by authors and Scientific Research Publishing Inc. This work is licensed under the Creative Commons Attribution International License (CC BY). http://creativecommons.org/licenses/by/4.0/ Abstract It is a very important significance to explore the bacterial typing methods rapidly, accurately and understand the genetic relation between isolates for controlling effectively the disease and pre- venting the dissemination and diffusion of the pathogen. The changes in the genetic material nucleotide sequences result in variation and evolution of bacteria, the development of molecular biology and genomics make typing of bacteria from phenotype to molecular typing. Riemerella anatipestifer is the causative agent of polyserositis of ducks and geese. We studied 54 isolates of Riemerella anatipestifer from Guangdong in China by multicolor sequence typing (MLST). The re- sult showed that 54 isolates of Riemerella anatipestifer were divided into 14 STs, and among E3, b12xiao and Cb1 three isolates have with independent of type STs.
    [Show full text]
  • Emerging Flavobacterial Infections in Fish
    Journal of Advanced Research (2014) xxx, xxx–xxx Cairo University Journal of Advanced Research REVIEW Emerging flavobacterial infections in fish: A review Thomas P. Loch a, Mohamed Faisal a,b,* a Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, 174 Food Safety and Toxicology Building, Michigan State University, East Lansing, MI 48824, USA b Department of Fisheries and Wildlife, College of Agriculture and Natural Resources, Natural Resources Building, Room 4, Michigan State University, East Lansing, MI 48824, USA ARTICLE INFO ABSTRACT Article history: Flavobacterial diseases in fish are caused by multiple bacterial species within the family Received 12 August 2014 Flavobacteriaceae and are responsible for devastating losses in wild and farmed fish stocks Received in revised form 27 October 2014 around the world. In addition to directly imposing negative economic and ecological effects, Accepted 28 October 2014 flavobacterial disease outbreaks are also notoriously difficult to prevent and control despite Available online xxxx nearly 100 years of scientific research. The emergence of recent reports linking previously uncharacterized flavobacteria to systemic infections and mortality events in fish stocks of Keywords: Europe, South America, Asia, Africa, and North America is also of major concern and has Flavobacterium highlighted some of the difficulties surrounding the diagnosis and chemotherapeutic treatment Chryseobacterium of flavobacterial fish diseases. Herein, we provide a review of the literature that focuses on Fish disease Flavobacterium and Chryseobacterium spp. and emphasizes those associated with fish. Coldwater disease ª 2014 Production and hosting by Elsevier B.V. on behalf of Cairo University. Flavobacteriosis Mohamed Faisal D.V.M., Ph.D., is currently a Thomas P.
    [Show full text]