DOCSLIB.ORG

Analysis of Procyanidins and Anthocyanins in Food Products Using Chromatographic and Spectroscopic Techniques

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text

Load more

Analysis of Procyanidins and Anthocyanins in Food Products using Chromatographic and Spectroscopic Techniques DISSERTATION Presented in Partial Fulfillment of the Requirements for the Degree Doctor of Philosophy in the Graduate School of The Ohio State University By Taylor Clyde Wallace Graduate Program in Food Science and Technology The Ohio State University 2010 Dissertation Committee: M. Monica Giusti, Advisor, Sheryl Barringer, Joshua Bomser, and John Litchfield Copyright by Taylor Clyde Wallace 2010 Abstract Procyanidins and anthocyanins are polyphenol compounds widely distributed throughout nature and are important because of their significant presence in the human diet and potential health benefits post consumption. Even though many food scientists, nutritionists, epidemiologists, and medical practitioners have related a decrease in many age and obesity related chronic diseases to the high consumption of fruits and vegetables containing these potent compounds / antioxidants, scientists have still yet to develop standardized protocols for quantification of procyanidins in food because of their structural complexity, polymerization, and stereochemistry. Procyanidins and anthocyanins are considered value added food ingredients in a variety of matrices. Depending on the food matrix, procyanidins, anthocyanins, and other polyphenols may or may not be desirable to the consumers. Monomer forms of procyanidins (flavan-3-ols such as catechin and epicatechin) provide a bitter flavor to food products, but become more astringent / less bitter as polymerization increases. Anthocyanins have a distinct influence on the consumer acceptance of a product because of their ability to produce a natural orange-red to blue-violet color. Consumer demand for healthier high quality food products has driven the industry to create food products with higher concentrations of procyanidins / anthocyanins and the need to develop better techniques for analysis of these phytonutrients. There is a need for increased understanding of the chemistry of these compounds in a variety of food matrices, as well as, the development of more reproducible methods for characterization, quantification, and authentication of these powerful phytonutrients for many purposes including validation of labeling claims. The long-term goal is to increase the knowledge and understanding of the role of ii procyanidins and anthocyanins in the diet and the many positive health benefits attributed to these compounds upon consumption by the consumer. The specific goal of this research was to develop better analytical methods for identification and quantification of procyanidins and anthocyanins in foods and to apply those methods to understand their chemistry in various food matrices, while monitoring the viability, stability, and integrity of these compounds to select processing conditions. To validate our hypothesis it was critical to evaluate parameters that affected the 4- dimethylaminocinnimaldehyde (DMAC) UV-visible spectrophotometric assay to help the industry develop a more reproducible and standardized protocol for the rapid quantification of proanthocyanidins. To better reproduce and assess total proanthocyanidins in a sample, it is our recommendation that when using catechin as a standard that the following parameters be applied: the use of 2% DMAC (w/v) and 6N H 2SO 4 to prepare the DMAC reagent, a reaction time of >15 min at constant room temperature between 21 and 25ºC, and the use of absolute methanol (<1% water in sample). Because there has been significant research conducted with various monomeric and oligomeric standards, our study focused on other parameters that influence the accuracy and reproducibility of the assay. The use of different standards in the assay has a significant effect on the color produced by the DMAC-proanthocyanidin complex. Catechin and epicatechin have been reported to have greater reactivity as compared to proanthocyanidin oligomers. Samples containing high concentrations of oligomeric proanthocyanidins may require a reaction time of up to 20 min as reported in other studies. Our studies as well as previous literature support that the color produced by the DMAC- proanthocyanidin complex is stable between 20 and 30 min for both monomers and oligomeric proanthocyanidins. The use of absolute methanol is necessary and involves removal of water until samples are dry and re-consituting the proanthocyanidins in methanol prior to analysis. The results from the parameters explored which effect the DMAC assay will aid the food industry in devoping a more reproducible and standard method for quantification and comparison proanthocyanidin contents in foods. ii Further evaluation of analytical techniques led us to explore chromatographic techniques to separate and identify procyanidins in cranberry extracts. Cranberries provide a unique opportunity to study both anthocyanins and procyanidins because they are prevalent in the American diet and contain a very diverse matrix of polyphenols, both A- and B-type procyanidins, high concentrations of the more oligomeric and polymeric procyanidins, and 6 non-acylated anthocyanins. Fluorescence and MS detection were used to confirm the identity and quantify procyanidins by their degree of polymerization up to nonamers. Both A- and B-type procyanidins were separated and quantified up to tetramers. Posing an additional barrier to this study, certain cranberry extracts were prepared with an insoluble inert resin, which actively binds polyphenols and aids in their concentration. As the industry has begun to utilize this compound to produce more concentrated phenolic extracts, development of better extraction techniques were explored to be able to better assess the procyanidin and anthocyanin contents of these products. Cranberry extracts containing the inert resin showed higher concentrations of total phenolics, monomeric anthocyanins, and procyanidins as compared to non-resin containing extracts. Commercial cranberry extracts contained between 0.73 and 22.45 mg/g procyanidins as quantified using HPLC – fluorescence detection. The use of Sephadex LH-20 was effective in eliminating interfering compounds prior to DMAC and HPLC analyses. The use of HPLC – fluorescence and MS detection proved to be the most accurate means of quantifying procyanidins in cranberries as compared to the DMAC assay. It was also shown that an acid / alkaline technique of removing the polyphenols from the inert resin degraded compounds, thus a 6X acetone / water extraction technique was developed in order to better assess the the quantity and profile of procyanidins present. Analytical advances in chromatography for anthocyanins were also achieved using HPLC – PDA and MS detection. Our final study utilized the analytical techniques prepared in the previous studies to solve an emerging industry / quality concern of unfermented cocoa powder extracts. Unfermented cocoa powder is produced with the intention of creating procyanidin-rich extracts used to increase the general public’s consumption of these healthy compounds. Because of the lack of iii fermentation, anthocyanins naturally present in cocoa beans remain viable producing an un- wanted violet color in the final extract. Our goal was to decolorize unfermented cocoa powder extracts while monitoring the stability, integrity, and viability of the procyanidins. The use of pH fluctuations, glycosidic enzymes, and potassium meta-bisulfite was explored to see which treatment best removed the violet color and preserved the other polyphenols present in the unfermented cocoa powder extracts. The use of bisulfite to bleach the anthocyanins present eliminated the violet color and preserved the composition and chemistry of the procyanidins and other phenolics present. Higher levels of bisulfite lead to an increased retention of anthocyanins in the final procyanidin-rich extract. The effective use of bisulfite to bleach the violet color present did not have a detrimental / significant effect on the stability of anthocyanins, procyanidins, or other polyphenols present in the procyanidin-rich unfermented cocoa powder extracts as compared to the control throughout a 60 day shelf life study. Our studies and this dissertation increase the understanding of the chemistry of anthocyanins / procyanidins and help to progress their many applications as value added ingredients. Indirectly, this research may also aid in the advancement of nutritional studies related to the health benefits of these powerful phytonutrients. iv Dedication Dedicated to my father Clyde F. Wallace Jr., to whom I love and owe so much more than this dissertation. Thank you for the many fights we had in high school over doing homework immediately after swim practice, calling me 20 times a day during finals week in college to make sure that I was studying, and being a solid foundation of support to me throughout graduate school. You have sacrificed, encouraged, and opened the door for me to so many opportunities that I never imagined possible. You are a role model to other single parents. I love you and Mamawl so much, and I hope that one day my son can see me as at least half of the person that you are to me. v Acknowledgements I wish to thank my advisor Dr. M. Monica Giusti, as well as, my dissertation committee Drs. Sheryl Barringer, Joshua Bomser, John Litchfield, and David Min for their guidance and intellectual support which made this dissertation possible. Monica you have crafted me into the scientist I am today and taught me the
Recommended publications
  • A Comparison of the Production of Polyphenol Contents and the Expression of Genes Involved in Vietnamese Tea Cultivars

    A Comparison of the Production of Polyphenol Contents and the Expression of Genes Involved in Vietnamese Tea Cultivars

    International Food Research Journal 26(6): 1781-1788 (December 2019) Journal homepage: http://www.ifrj.upm.edu.my A comparison of the production of polyphenol contents and the expression of genes involved in Vietnamese tea cultivars 1Hoang, T. T. Y., 2Luu, H. L., 2Nguyen, T. L., 3Duong, T. D., 4,5Nguyen, H. D. and 2*Huynh, T. T. H 1Thai Nguyen University of Sciences, Thai Nguyen University, Thai Nguyen Province 24000, Vietnam 2Institute of Genome Research, Vietnam Academy of Science and Technology (VAST), Hanoi 100000, Vietnam 3Thai Nguyen University of Agriculture and Forestry, Thai Nguyen University, Thai Nguyen Province 24000, Vietnam 4Advanced Centre for Bioorganic Chemistry, Institute of Marine Biochemistry, VAST, Hanoi 100000, Vietnam 5University of Science and Technology of Hanoi, VAST, Hanoi 100000, Vietnam Article history Abstract Received: 19 June, 2019 Tea (Camellia sinensis) is a popular health beverage which is consumed all over the world Received in revised form: due to its good aroma and taste. Tea consumption is also considered to reduce the risk of 16 September, 2019 several diseases in humans, including cardiovascular diseases, diabetes and cancers. Recent Accepted: 25 September, 2019 studies have shown that polyphenols derived from tea may contribute to the majority of these pharmaceutical properties. Among all the tea polyphenols, catechins are the main components that include (−)-epicatechin (EC), (−)-epicatechin gallate (ECG), (−)-epigallocatechin (EGC), (−)-epigallocatechin-3 gallate (EGCG), (+)-catechin (C), (−)-catechin gallate (CG), (−)-gallocatechin (GC), and (−)-gallocatechingallate (GCG). In the present work, four Keywords catechins (C, EGC, ECG, and EGCG) and two anthocyanidins (cyanidin 3-O-glucoside and delphinidin 3-O-glucoside) in two Vietnamese tea cultivars, Trungduxanh and Trungdutim, were Catechin LAR quantitatively detected by high-performance liquid chromatography.
  • Health-Promoting Effects of Traditional Foods

    Health-Promoting Effects of Traditional Foods

    Health-PromotingFoodsTraditional Effects of • Marcello Iriti Health-Promoting Effects of Traditional Foods Edited by Marcello Iriti Printed Edition of the Special Issue Published in Foods Health-Promoting Effects of Traditional Foods Health-Promoting Effects of Traditional Foods Editor Marcello Iriti MDPI • Basel • Beijing • Wuhan • Barcelona • Belgrade • Manchester • Tokyo • Cluj • Tianjin Editor Marcello Iriti Department of Agricultural and Environmental Sciences, Milan State University Italy Editorial Office MDPI St. Alban-Anlage 66 4052 Basel, Switzerland This is a reprint of articles from the Special Issue published online in the open access journal Foods (ISSN 2304-8158) (available at: https://www.mdpi.com/journal/foods/special issues/health effects traditional foods). For citation purposes, cite each article independently as indicated on the article page online and as indicated below: LastName, A.A.; LastName, B.B.; LastName, C.C. Article Title. Journal Name Year, Article Number, Page Range. ISBN 978-3-03943-312-4 (Hbk) ISBN 978-3-03943-313-1 (PDF) c 2020 by the authors. Articles in this book are Open Access and distributed under the Creative Commons Attribution (CC BY) license, which allows users to download, copy and build upon published articles, as long as the author and publisher are properly credited, which ensures maximum dissemination and a wider impact of our publications. The book as a whole is distributed by MDPI under the terms and conditions of the Creative Commons license CC BY-NC-ND. Contents About the Editor .............................................. vii Marcello Iriti, Elena Maria Varoni and Sara Vitalini Healthy Diets and Modifiable Risk Factors for Non- Communicable Diseases—The European Perspective Reprinted from: Foods 2020, 9, 940, doi:10.3390/foods9070940 ....................
  • The Use of Plants in the Traditional Management of Diabetes in Nigeria: Pharmacological and Toxicological Considerations

    The Use of Plants in the Traditional Management of Diabetes in Nigeria: Pharmacological and Toxicological Considerations

    Journal of Ethnopharmacology 155 (2014) 857–924 Contents lists available at ScienceDirect Journal of Ethnopharmacology journal homepage: www.elsevier.com/locate/jep Review The use of plants in the traditional management of diabetes in Nigeria: Pharmacological and toxicological considerations Udoamaka F. Ezuruike n, Jose M. Prieto 1 Center for Pharmacognosy and Phytotherapy, Department of Pharmaceutical and Biological Chemistry, School of Pharmacy, University College London, 29-39 Brunswick Square, WC1N 1AX London, United Kingdom article info abstract Article history: Ethnopharmacological relevance: The prevalence of diabetes is on a steady increase worldwide and it is Received 15 November 2013 now identified as one of the main threats to human health in the 21st century. In Nigeria, the use of Received in revised form herbal medicine alone or alongside prescription drugs for its management is quite common. We hereby 26 May 2014 carry out a review of medicinal plants traditionally used for diabetes management in Nigeria. Based on Accepted 26 May 2014 the available evidence on the species' pharmacology and safety, we highlight ways in which their Available online 12 June 2014 therapeutic potential can be properly harnessed for possible integration into the country's healthcare Keywords: system. Diabetes Materials and methods: Ethnobotanical information was obtained from a literature search of electronic Nigeria databases such as Google Scholar, Pubmed and Scopus up to 2013 for publications on medicinal plants Ethnopharmacology used in diabetes management, in which the place of use and/or sample collection was identified as Herb–drug interactions Nigeria. ‘Diabetes’ and ‘Nigeria’ were used as keywords for the primary searches; and then ‘Plant name – WHO Traditional Medicine Strategy accepted or synonyms’, ‘Constituents’, ‘Drug interaction’ and/or ‘Toxicity’ for the secondary searches.
  • Solutions That Meet Your Demands for Food Testing & Agriculture

    Solutions That Meet Your Demands for Food Testing & Agriculture

    Solutions that meet your demands for food testing & agriculture Our measure is your success. Excellent choices for food & agriculture applications products I applications I software I services Agilent Technologies Consumer Products Toys, jewelry, clothing, and other products are frequently recalled due to the presence of unsafe levels of substances such as lead from paint and phthal- ates from product polymers and packaging. Whether your perspective is to guarantee your products are free of contaminants or you are screening for harmful contaminants in a wide variety of consumer products, Agilent Tech- nologies provides the tools you need to detect and measure these and other harmful contaminants. > Search entire document Agilent 1290 Infinity LC with Agilent Poroshell columns for simultaneous determination of eight organic UV filters in under two minutes Application Note Consumer Products Authors Siji Joseph Agilent Technologies India Pvt. Ltd. mAU Amino benzoic acid Bangalore, India 2 Oxybenzone 1.5 4-Methyl benzylidene camphor Dioxybenzone Avobenzone Michael Woodman 1 Octyl methoxycinnamate 0.5 Octocrylene Agilent Technologies, Inc. Octyl salicylate 2850 Centerville Road 0 0 0.5 1 1.5 2 min Wilmington DE 19808 USA Abstract Levels of UV filters in personal care products are regulated by the FDA and European Pharmacopeia (EP). Liquid chromatographic (LC) methods are widely accepted analyt- ical techniques for the qualitative and quantitative analysis of these UV filters. Most of these traditional LC methods require about 25–50 minutes. In this Application Note, the Agilent 1290 Infinity LC, in combination with Agilent Poroshell columns, were used for development of a short, sensitive, robust and well resolved separation of eight FDA/EP approved active UV filter ingredients in 99 seconds.
  • L.) Leaves Tao Jiang1,3, Kunyuan Guo2,3, Lingdi Liu1, Wei Tian1, Xiaoliang Xie1, Saiqun Wen1 & Chunxiu Wen1*

    L.) Leaves Tao Jiang1,3, Kunyuan Guo2,3, Lingdi Liu1, Wei Tian1, Xiaoliang Xie1, Saiqun Wen1 & Chunxiu Wen1*

    www.nature.com/scientificreports OPEN Integrated transcriptomic and metabolomic data reveal the favonoid biosynthesis metabolic pathway in Perilla frutescens (L.) leaves Tao Jiang1,3, Kunyuan Guo2,3, Lingdi Liu1, Wei Tian1, Xiaoliang Xie1, Saiqun Wen1 & Chunxiu Wen1* Perilla frutescens (L.) is an important medicinal and edible plant in China with nutritional and medical uses. The extract from leaves of Perilla frutescens contains favonoids and volatile oils, which are mainly used in traditional Chinese medicine. In this study, we analyzed the transcriptomic and metabolomic data of the leaves of two Perilla frutescens varieties: JIZI 1 and JIZI 2. A total of 9277 diferentially expressed genes and 223 favonoid metabolites were identifed in these varieties. Chrysoeriol, apigenin, malvidin, cyanidin, kaempferol, and their derivatives were abundant in the leaves of Perilla frutescens, which were more than 70% of total favonoid contents. A total of 77 unigenes encoding 15 enzymes were identifed as candidate genes involved in favonoid biosynthesis in the leaves of Perilla frutescens. High expression of the CHS gene enhances the accumulation of favonoids in the leaves of Perilla frutescens. Our results provide valuable information on the favonoid metabolites and candidate genes involved in the favonoid biosynthesis pathways in the leaves of Perilla frutescens. Perilla frutescens (L.), which is a self-compatible annual herb, belongs to the family Lamiaceae. Tis species has been widely cultivated in China, Japan, and Korea for centuries. Perilla frutescens is an important medicinal and edible plant in China with medical and nutritional uses 1. Its leaves can be utilized as a transitional medicinal herb, as a vegetable, and as a spice, and its seeds can be processed into foods and nutritional edible oils 2.
  • A Combined Experimental and Computational Study of Chrysanthemin As a Pigment for Dye-Sensitized Solar Cells

    A Combined Experimental and Computational Study of Chrysanthemin As a Pigment for Dye-Sensitized Solar Cells

    molecules Article A Combined Experimental and Computational Study of Chrysanthemin as a Pigment for Dye-Sensitized Solar Cells Atoumane Ndiaye 1,2 , Alle Dioum 1, Corneliu I. Oprea 2 , Anca Dumbrava 3,* , Jeanina Lungu 2, Adrian Georgescu 2, Florin Moscalu 2, Mihai A. Gîr¸tu 2,* , Aboubaker Chedikh Beye 1 and Issakha Youm 1,* 1 Department of Physics, Cheikh Anta Diop University of Dakar, 5005 Dakar-Fann, Senegal; [email protected] (A.N.); [email protected] (A.D.); [email protected] (A.C.B.) 2 Department of Physics, Ovidius University of Constanta, 900527 Constanta, Romania; [email protected] (C.I.O.); [email protected] (J.L.); [email protected] (A.G.); fl[email protected] (F.M.) 3 Department of Chemistry and Chemical Engineering, Ovidius University of Constanta, 900527 Constanta, Romania * Correspondence: [email protected] (A.D.); [email protected] (M.A.G.); [email protected] (I.Y.) Abstract: The theoretical study of chrysanthemin (cyanidin 3-glucoside) as a pigment for TiO2-based dye-sensitized solar cells (DSSCs) was performed with the GAUSSSIAN 09 simulation. The electronic spectra of neutral and anionic chrysanthemin molecules were calculated by density functional theory with B3LYP functional and DGDZVP basis set. A better energy level alignment was found for partially deprotonated molecules of chrysanthemin, with the excited photoelectron having enough energy in order to be transferred to the conduction band of TiO2 semiconductor in DSSCs. In addition, we used the raw aqueous extracts of roselle (Hibiscus sabdariffa) calyces as the source of chrysanthemin Citation: Ndiaye, A.; Dioum, A.; and the extracts with various pH values were tested in DSSCs.
  • Nutraceutical Components, Antioxidant Activity, and Color of 11 Varieties of Prickly Pear (Opuntia Sp.) 1 3 1* 2 M

    Nutraceutical Components, Antioxidant Activity, and Color of 11 Varieties of Prickly Pear (Opuntia Sp.) 1 3 1* 2 M

    Journal of Applied Botany and Food Quality 91, 211 - 218 (2018), DOI:10.5073/JABFQ.2018.091.028 1*Instituto de Horticultura, Universidad Autónoma Chapingo, Carretera México-Texcoco, Chapingo, Estado de México, México 2Instituto de Alimentos, Universidad Autónoma Chapingo, Carretera México-Texcoco, Chapingo, Estado de México, México 3Tecnológico Nacional de México, Instituto Tecnológico de Conkal, Avenida Tecnológico s/n Conkal, Yucatán, México Nutraceutical components, antioxidant activity, and color of 11 varieties of prickly pear (Opuntia sp.) 1 3 1* 2 M. Ramírez-Ramos , K. Medina-Dzul , R. García-Mateos , J. Corrales-García , C. Ybarra-Moncada2, A.M. Castillo-González1 (Submitted: October 13, 2017; Accepted: June 26, 2018) Summary tive diseases, due to the presence of certain metabolites (phenolic compounds, flavonoids, ascorbic acid, and betalains) (SUMAYA- In Mexico, there are 50 recorded varieties of the prickly pear fruit. MARTÍNEZ et al., 2011; ABOU-ELELLA and ALI, 2014). Biosynthetically, National production covers only the white-pulp fruit, but other betalamic acid-derived betalains, which are pigments used in the red varieties have export potential; however, their nutraceutical agri-food industry as natural colorants (STRACK et al., 2003), also properties are unknown. The pulp and peel (underutilized tissue) have antioxidant properties (LIVREA and TESORIERE, 2006; ALBANO of the pigmented fruits of the genus Opuntia sp. are marketed on a et al., 2015). Moreover, color is part of fruit quality and may therefore limited basis. They represent an alternative source of stable pigments be a factor in its preference, acceptance, or rejection, and play a (betalains), which are associated with antioxidant properties, for decisive role in the failure or success of prickly pear marketing.
  • Characterization and Biological Activity of Condensed Tannins from Tropical Forage Legumes

    Characterization and Biological Activity of Condensed Tannins from Tropical Forage Legumes

    1070 T.P. Pereira et al. Characterization and biological activity of condensed tannins from tropical forage legumes Tatiana Pires Pereira(1), Elisa Cristina Modesto(1), Delci de Deus Nepomuceno(2), Osniel Faria de Oliveira(3), Rafaela Scalise Xavier de Freitas(1), James Pierre Muir(4), José Carlos Batista Dubeux Junior(5) and João Carlos de Carvalho Almeida(1) (1)Universidade Federal Rural do Rio de Janeiro, Rodovia BR-465, Km 07, s/no, Zona Rural, CEP 23890-000 Seropédica, RJ, Brazil. E-mail: [email protected], [email protected], [email protected], [email protected] (2)In memoriam (3)Universidade Federal Rural de Pernambuco, Dois Irmãos, CEP 52171-900 Recife, PE, Brazil. E-mail: [email protected] (4)Texas AgriLife Research and Extension Center, Stephenville, TX, USA. E-mail: [email protected] (5)University of Florida, North Florida Research and Education Center, Highway, Marianna, FL, USA. E-mail: [email protected] Abstract – The objective of this work was to characterize condensed tannins (CT) from six tropical forage legumes and to determine their biological activity. The monomers propelargonidin, prodelphinidin and procyanidin were analyzed, as well as extractable condensed tannin (ECT), protein-bound CT (PBCT) and fiber-bound CT (FBCT), molecular weight, degree of polymerization, polydispersity index, and biological activity by protein precipitate by phenols (PPP) of leaves of the legumes Cajanus cajan, Gliricidia sepium, Stylosanthes capitata x Stylosanthes macrocephala (stylo), Flemingia macrophylla, Cratylia argentea, and Mimosa caesalpiniifolia, and of the bark of this latter species. Differences were observed in the concentrations of ECT, PBCT, PPP, and total condensed tannin among species, but not in that of FBCT.
  • Determination of Major Anthocyanin

    Determination of Major Anthocyanin

    02_benmeziane_05b-tomazic 13/10/16 13:03 Page125 DETERMINATION OF MAJOR ANTHOCYANIN PIGMENTS AND FLAVONOLS IN RED GRAPE SKIN OF SOME TABLE GRAPE VARIETIES ( VITIS VINIFERA SP.) BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY–PHOTODIODE ARRAY DETECTION (HPLC-DAD) Farida BENMEZIANE 1* , Yves CADOT 2, Rachid DJAMAI 3 and Lynda DJERMOUN 4 1: Department of Agronomy, University of El-Tarf, PB 73, El-Tarf 36000, Algeria 2: Institut de Recherche en Horticulture et Semences UMR1345 (INRA / Agrocampus-Ouest / Université d’Angers), 42 rue Georges Morel BP 60057, 49071 Beaucouzé Cedex, France 3: Department of Biology, University of Badji-Mokhtar, Annaba, PB 12, Annaba 23000, Algeria 4: University A. Mira of Bejaia, Faculty of Sciences of Nature and Life, Department of Food Science, 3BS Laboratory, Bejaia 06000, Algeria Abstract Aim : The aim of this study was the investigation of the anthocyanin and flavonol content in grape skin extracts. Five prevalent anthocyanin-types and four flavonol-types were determined in the skin of three red table grape varieties widely cultivated in El- Tarf (Algeria). Methods and results : The identification of the compounds was performed by HPLC-DAD based on C-18 reversed phase column separation. Results from HPLC analysis showed that malvidin and petunidin-3- O-glucoside were the major anthocyanin glucoside, whereas quercetin-3- O-glucoside was the major flavonol among the four identified. Conclusion : The content of anthocyanins and flavonols in the grape skin of three grapevine ( Vitis vinifera ) varieties exhibits notable differences among the cultivars studied, confirming their importance in the varietal characterization. The highest concentrations of total anthocyanins and flavonols corresponded to the Gros noir variety.
  • Ultrasound-Assisted Extraction Optimization Using

    Ultrasound-Assisted Extraction Optimization Using

    a ISSN 0101-2061 (Print) Food Science and Technology ISSN 1678-457X (Online) DOI: https://doi.org/10.1590/fst.13421 Berberis crataegina DC. as a novel natural food colorant source: ultrasound-assisted extraction optimization using response surface methodology and thermal stability studies Mehmet DEMIRCI1,2 , Merve TOMAS1 , Zeynep Hazal TEKIN-ÇAKMAK2 , Salih KARASU2* Abstract This study aimed to investigate the potential use of anthocyanin of Berberis crataegina DC. as a natural food coloring agent in the food industry. For this aim, the ultrasound-assisted extraction (UAE) method was performed to extract anthocyanin of Berberis crataegina DC. The effect of ultrasound power 1(X : 20-100%), extraction temperature (X2: 20-60 °C), and time (X3: 10-20 min) on TPC and TAC of Berberis crataegina DC. extracts were examined and optimized by applying the Box–Behnken experimental design (BBD) with the response surface methodology (RSM). The influence of three independent variables and their combinatorial interactions on TPC and TAC were investigated by the quadratic models (R2: 0.9638&0.9892 and adj R2:0.9171&0.9654, respectively). The optimum conditions were determined as the amplitude level of 98%, the temperature of 57.41 °C, and extraction time of 13.86 min. The main anthocyanin compounds were identified, namely, Delphinidin-3-O- galactoside, Cyanidin-3-O-glucoside, Cyanidin-3-O-rutinoside, Petunidin-3-O-glucoside, Pelargonidin-3-O-glucoside, and Peonidin-3-O-glucoside. The anthocyanin degradation showed first-order kinetic, degradation rate constant (k), the half-life values (t1/2), and loss (%) were significantly affected by different temperatures (P < 0.05).
  • Determination of Total Procyanidins in Selected Chocolate and Confectionery Products Using DMAC

    Determination of Total Procyanidins in Selected Chocolate and Confectionery Products Using DMAC

    SPSFAM-FLAV-11 Based from Call for Methods 12-21-2011 PAYNE ET AL.: JOURNAL OF AOAC INTERNATIONAL VOL. 93, NO. 1, 2010 89 DIETARY SUPPLEMENTS Determination of Total Procyanidins in Selected Chocolate and Confectionery Products Using DMAC MARK J. PAYNE,WILLIAM JEFFREY HURST,andDAVI D A. STUART Hershey Center for Health and Nutrition, The Hershey Co., 1025 Reese Ave, Hershey, PA 17033 BOXIN OU and ELLEN FAN Brunswick Laboratories (USA), 50 Commerce Way, Norton, MA 02766 HONGPING JI and YAN KOU Brunswick Laboratories (China), 218 Xing Hu Rd, Suzhou Industrial Park, China 215125 A simple, specific, high-throughput colorimetric which the monomers are linked through C4÷C8 or, less method based on the reaction of frequently, C4÷C6 linkages. In the less common A-type 4-dimethylaminocinnamaldehyde (DMAC) with procyanidins, the monomers are connected through C2÷O÷C7 flavan-3-ols was developed to determine total or C2÷O÷C5 linkages. Procyanidins are widely distributed in procyanidins in selected cacao-based products. plants and are found in significant quantities in foods such as Extracts of defatted samples were dispensed into a fruits, spices, tea, wine, nuts, and cocoa (1). Of the many types of 96-well plate and reacted with DMAC. The polyphenols, flavan-3-ols, flavonols, and anthocyanidins are the absorbance of the reaction products was most abundant classes found in plants (2). The interest in measured at 640 nm and compared to polyphenol antioxidants has increased dramatically due to their commercially available procyanidin B2 as a ability to scavenge free radicals and their association with a standard.
  • Intereferents in Condensed Tannins Quantification by the Vanillin Assay

    Intereferents in Condensed Tannins Quantification by the Vanillin Assay

    INTEREFERENTS IN CONDENSED TANNINS QUANTIFICATION BY THE VANILLIN ASSAY IOANNA MAVRIKOU Dissertação para obtenção do Grau de Mestre em Vinifera EuroMaster – European Master of Sciences of Viticulture and Oenology Orientador: Professor Jorge Ricardo da Silva Júri: Presidente: Olga Laureano, Investigadora Coordenadora, UTL/ISA Vogais: - Antonio Morata, Professor, Universidad Politecnica de Madrid - Jorge Ricardo da Silva, Professor, UTL/ISA Lisboa, 2012 Acknowledgments First and foremost, I would like to thank the Vinifera EuroMaster consortium for giving me the opportunity to participate in the M.Sc. of Viticulture and Enology. Moreover, I would like to express my appreciation to the leading universities and the professors from all around the world for sharing their scientific knowledge and experiences with us and improving day by day the program through mobility. Furthermore, I would like to thank the ISA/UTL University of Lisbon and the personnel working in the laboratory of Enology for providing me with tools, help and a great working environment during the experimental period of this thesis. Special acknowledge to my Professor Jorge Ricardo Da Silva for tutoring me throughout my experiment, but also for the chance to think freely and go deeper to the field of phenols. Last but most important, I would like to extend my special thanks to my family and friends for being a true support and inspiration in every doubt and decision. 1 UTL/ISA University of Lisbon “Vinifera Euromaster” European Master of Science in Viticulture&Oenology Ioanna Mavrikou: Inteferents in condensed tannins quantification with vanillin assay MSc Thesis: 67 pages Key Words: Proanthocyanidins; Interference substances; Phenols; Vanillin assay Abstract Different methods have been established in order to perform accurately the quantification of the condensed tannins in various plant products and beverages.