Screening of Medicinal and Edible Plants in Okinawa, Japan, for Enhanced Proliferative and Collagen Synthesis Activities in NB1RGB Human Skin Fibroblast Cells
Biosci. Biotechnol. Biochem., 76 (12), 2317–2320, 2012 Note Screening of Medicinal and Edible Plants in Okinawa, Japan, for Enhanced Proliferative and Collagen Synthesis Activities in NB1RGB Human Skin Fibroblast Cells y Makoto TAKAHASHI,1 Yonathan ASIKIN,2 Kensaku TAKARA,1 and Koji WADA1; 1Department of Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus, Okinawa 903-0213, Japan 2Course of Biochemistry and Applied Bioscience, United Graduate School of Agricultural Science, Kagoshima University, Kagoshima 890-0065, Japan Received June 21, 2012; Accepted August 21, 2012; Online Publication, December 7, 2012 [doi:10.1271/bbb.120478] To identify plants with bioactive potential for skin survey the additional medicinal characteristics of these care, methanol extracts of 56 plant parts from 47 plants, we evaluated the effects of the Okinawan plant medical and edible plants cultivated in Okinawa were extracts on cell proliferation and type I collagen syn- tested for their proliferative effects on NB1RGB skin thesis in human skin cell lines. fibroblast cells. Extracts from six plants, Bischofia A total of 56 plant parts from 47 species collected javanica, Colocasia esculenta, Melaleuca alternifolia, from the University of Ryukyus campus (Okinawa, Piper angustifolia, Jasminum sambac, and Curcuma Japan) are listed in Table 1. Each dried plant part (2 g) longa, showed higher NB1RGB cell proliferation activ- was ground using a mini-grinder (Model DF-15, ity (>10%) than the control, at various concentrations. Shenzhen Laitong Co., Shenzhen, China), and then Among the six extracts, only the C. longa extract caused soaked in 20 mL of methanol at room temperature for an increase in collagen synthesis in NB1RGB cells, as 24 h.
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