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Altered Glycosylation in Pancreatic Cancer: Development of New Tumor Markers and Therapeutic Strategies

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Altered Glycosylation in Pancreatic Cancer: Development of New Tumor Markers and Therapeutic Strategies ALTERED GLYCOSYLATION IN PANCREATIC CANCER: DEVELOPMENT OF NEW TUMOR MARKERS AND THERAPEUTIC STRATEGIES Pedro Enrique Guerrero Barrado Per citar o enllaçar aquest document: Para citar o enlazar este documento: Use this url to cite or link to this publication: http://hdl.handle.net/10803/671007 ADVERTIMENT. L'accés als continguts d'aquesta tesi doctoral i la seva utilització ha de respectar els drets de la persona autora. Pot ser utilitzada per a consulta o estudi personal, així com en activitats o materials d'investigació i docència en els termes establerts a l'art. 32 del Text Refós de la Llei de Propietat Intel·lectual (RDL 1/1996). Per altres utilitzacions es requereix l'autorització prèvia i expressa de la persona autora. En qualsevol cas, en la utilització dels seus continguts caldrà indicar de forma clara el nom i cognoms de la persona autora i el títol de la tesi doctoral. No s'autoritza la seva reproducció o altres formes d'explotació efectuades amb finalitats de lucre ni la seva comunicació pública des d'un lloc aliè al servei TDX. Tampoc s'autoritza la presentació del seu contingut en una finestra o marc aliè a TDX (framing). Aquesta reserva de drets afecta tant als continguts de la tesi com als seus resums i índexs. ADVERTENCIA. El acceso a los contenidos de esta tesis doctoral y su utilización debe respetar los derechos de la persona autora. Puede ser utilizada para consulta o estudio personal, así como en actividades o materiales de investigación y docencia en los términos establecidos en el art. 32 del Texto Refundido de la Ley de Propiedad Intelectual (RDL 1/1996). Para otros usos se requiere la autorización previa y expresa de la persona autora. En cualquier caso, en la utilización de sus contenidos se deberá indicar de forma clara el nombre y apellidos de la persona autora y el título de la tesis doctoral. No se autoriza su reproducción u otras formas de explotación efectuadas con fines lucrativos ni su comunicación pública desde un sitio ajeno al servicio TDR. Tampoco se autoriza la presentación de su contenido en una ventana o marco ajeno a TDR (framing). Esta reserva de derechos afecta tanto al contenido de la tesis como a sus resúmenes e índices. WARNING. Access to the contents of this doctoral thesis and its use must respect the rights of the author. It can be used for reference or private study, as well as research and learning activities or materials in the terms established by the 32nd article of the Spanish Consolidated Copyright Act (RDL 1/1996). Express and previous authorization of the author is required for any other uses. In any case, when using its content, full name of the author and title of the thesis must be clearly indicated. Reproduction or other forms of for profit use or public communication from outside TDX service is not allowed. Presentation of its content in a window or frame external to TDX (framing) is not authorized either. These rights affect both the content of the thesis and its abstracts and indexes. DOCTORAL THESIS Altered glycosylation in pancreatic cancer: development of new tumor markers and therapeutic strategies Pedro Enrique Guerrero Barrado 2020 DOCTORAL THESIS Altered glycosylation in pancreatic cancer: development of new tumor markers and therapeutic strategies Pedro Enrique Guerrero Barrado 2020 Doctoral Programme in Molecular Biology, Biomedicine and Health Thesis supervisors: Dr. Rosa Peracaula Miró Dr. Esther Llop Escorihuela Tutor: Dr. Rosa Peracaula Miró PhD thesis submitted in fulfilment of the requirement to for the title of doctor from the University of Girona Certificate of thesis direction Hereby, Dr. Rosa Peracaula Miró and Dr. Esther Llop Escorihuela from the University of Girona DECLARE: That the work entitled Altered glycosylation in pancreatic cancer: development of new tumor markers and therapeutic strategies, which presents Pedro Enrique Guerrero Barrado to obtain the title of doctor, has been carried out under our direction and that it meets the requirements to be eligible for an International Mention. And for the record and have the appropriate effects, we sign this document. For all intents and purposes, the following certification is signed: Dr. Rosa Peracaula Miró Dr. Esther Llop Escorihuela Girona, June 2020 Agradecimientos En primer lugar, me gustaría dar las gracias a mis dos directoras de tesis Esther y Rosa que han hecho posible que hoy pueda estar escribiendo esta tesis. Por todo lo que me han enseñado desde ese primer contacto de prácticas de empresa con nuestras columnas de SNA. Gracias por vuestra paciencia, por todo el apoyo científico y personal recibido en los momentos buenos y en los de ofuscación con la tesis. Agradeceros por todo lo enseñado, por las oportunidades que me habéis dado para continuar aprendiendo cosas nuevas en sitios tan diferentes. Gracias por hacer que en estos años fuera de casa estando en el trabajo me sintiera como en casa y por ponerle ruedas a la tesis aunque hubiera unos cuantos kilómetros o una cuarentena por medio. ¡¡Lo de hacer frases más cortas todavía lo tengo que practicar más Esther!! Pero por lo demás, he aprendido mucho de vosotras. Por supuesto agradecer a todos los miembros que forman o han formado parte de Bioquímica del cáncer (BQ-105 para los amigos) con l@s cuales he coincidido estos años. A Rafa, mil gracias por acabar siempre consiguiéndome las firmas antes del deadline por muy justos que fuéramos y por darme la oportunidad de poder ver como han ido creciendo cosas del TFG e involucrarme en ellas y la ilusión que me ha hecho! Que nada te quite tú buen humor y esas risas que se escuchaban desde el pasillo que animan a todos. A Mª Ángeles porque gracias a ella tuve la oportunidad de colaborar con el grupo, Silvia por esos consejillos con las 2D, Anna por la ayuda por cultivos y por supuesto especialmente dar gracias a Montse, por esa actitud y alegría que transmites, por todos esos consejos, por la de veces que me has ayudado, por todas las charlas y las horas de laboratorio mano a mano que he tenido la suerte de pasar como compi de poyata. Txell que no me olvido tampoco!!! por ser la alegría de la huerta, nuestro radiopatio y nuestra social manager de los congresos. Ni que decir de mis compis de despacho,,,,, que han tenido la paciencia de aguantar mis tonterías todos estos años, hasta el punto de colgar un ola k ase del techo incluso con outfit navideño, por dejarme llenar el despacho de telarañas en Halloween e ir colonizando la mesa a modo de vivero privado. Muchísimas Gracias Adrià, Anna y Laura por hacer tan ameno todos estos años, por todas nuestras tonterías y el buen rollo que transmitís, por todos esos momentazos que solo recordar te sacan la risa tonta, como nuestra traicionera, nuestros paseos en unicornio XXL por la playa, las bromas saliendo de las cajas, nuestro cultivo in vitro de orquídeas y algún que otro petardo con hielo seco siempre en el momento mas inoportuno del mundo por supuesto. Gracias a nuestros vecinos Bioquímicos, Alex por el buen rollo que transmites, Jess, Santi, Imma por ese buen carácter y estar siempre ahí para echar una mano. Gracias a todo el granja team por hacer las comidas tan amenas, por todos esos escape rooms de los cuales hemos salido a tiempo y todas esas experiencias y cenitas de becarios, por ponerle banda sonora a la barca de rafting o nuestros ataques a la máquina de hielo para las escapadas playeras. Micro team, Elianita por ser siempre tan detallista y compartir las penas y estrés estos últimos meses con todos los papeleos de la tesis y la burocracia; ¡¡¡Qué por fin vemos la luz!!! Mireia, gracias por siempre estar dispuesta a echar una mano en lo que haga falta y ser el SOS de las dudas ya sean del sepe, protocolos, papeleo o lo que haga falta, ni Wikipedia puede competir,,,, Eli, Elena, Queralt, Sara, Nuria, Paola, Carla y Pau en el pack por ese buen rollo y risas que hemos compartido, Pau siempre tan sarcástico y sacándole punta a todo animando el cotarro. Siempre me acordare con cariño de ese san Juan tan especial en los tipis con Carla sufriendo con mi hoguera. Biocels y neuro, Irene y Sandra, daros las gracias por vuestra paciencia con mi nivel de catalán y las risas que me habéis sacado, por ir muchas más veces a la playa con cervezas con limón sin alcohol Sandrita. Iker y sus chupitos de licor de oso consiguiendo que te lo pases bien hasta encerrados en un ascensor y a las genéticas Judith y Melania y a todos los miembros del departamento que en algún momento u otro me han ayudado todos estos años. Agradecer también a todo el equipo del IMIM, Carlos, Joan, Héctor, Mireia y con especial cariño a Pilar y Neus por todo lo que me habéis enseñado, las innumerables ayudas y las bases que me habéis brindado, Snails incluidos. Gemma, Aida por la ayuda siempre con los protocolos y las becas. Ni que decir de la mejor científica que ha pisado el IMIM y mi corresponsal de Barcelona, Mrs galectina la doctora Vinaixa, por la de veces que me ha tenido que ayudar de emergencia y mandar pantallazos de su libreta, por tu carácter, por ser la alegría de la huerta, por esas risas que siempre sacas a quien te rodea, por ese apoyo en los momentos mas flojos, por las anécdotas, por esas invitaciones a valencia con Paella de verdad incluida y alguna que otra noche de fiesta. Muchas gracias es quedarse corto.
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