Bub3 Polyclonal Antibody

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PRODUCT DATA SHEET Bioworld Technology,Inc. Bub3 polyclonal antibody Catalog: BS90151 Host: Rabbit Reactivity: Human, Mouse, Rat BackGround: Specificity: BUB3 (budding uninhibited by benzimidazoles 3 homo- Bub3 polyclonal antibody detects endogenous levels of log), also known as BUB3L or hBUB3, is a conserved Bub3 protein. component of the mitotic spindle assembly complex DATA: (MCC). It contains five WD repeat domains and forms cell cycle constitutive complexes with BUB1 and BUBR1. BUB3 is essential for the kinetochore localiza- tion of BUB1 and BUBR1. As a component of the MCC, BUB3 is involved in the essential spindle checkpoint pathway that operates during early embryogenesis. The spindle checkpoint pathway functions to postpone the ini- tiation of anaphase until chromosomes are properly at- Western blot analysis of Bub3 on different lysates using anti-Bub3 anti- tached to the spindle. This acts to ensure accurate chro- body at 1/500 dilution. mosome segregation. In addition, BUB3 plays a role in Positive control: regulating the establishment of correct kineto- Lane 1: A549 chore-microtubule attachments. BUB3 is also thought to Lane 2: HL-60 bind Tctex1L (or DYNLT3), a dynein light chain. Lane 3: A431 Product: Lane 4: Rat colon Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Molecular Weight: 37 kDa Swiss-Prot: O43684(Human) Q9WVA3(Mouse) Purification&Purity: ICC staining Bub3 in A431 cells (green). The nuclear counter stain is ProA affinity purified DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with Applications: 0.25% Triton X100/PBS. WB:1:500-1:2,000 Note: ICC:1:50-1:200 For research use only, not for use in diagnostic procedure. IHC:1:50-1:200 IP:1:10-1:50 FC:1:50-1:100 Storage&Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles. Bioworld Technology, Inc. Bioworld technology, co. Ltd. Add： 1660 South Highway 100, Suite 500 St. Louis Park, Add： No 9, weidi road Qixia District Nanjing, 210046, MN 55416,USA. P. R. China. Email: [email protected] Email: [email protected] Tel: 6123263284 Tel: 0086-025-68037686 Fax: 6122933841 Fax: 0086-025-68035151 .

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A Computational Approach for Defining a Signature of Β-Cell Golgi Stress in Diabetes Mellitus
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Efficient Professional Protein and Antibody Platforms BUB3 Antibody Basic information: Catalog No.: UPA63666 Source: Rabbit Size: 50ul/100ul Clonality: Monoclonal Concentration: 1mg/ml Isotype: Rabbit IgG Purification: Protein A affinity purified Useful Information: WB:1:500-1:2000 ICC:1:50-1:200 Applications: IHC:1:50-1:200 IP:1:10-1:50 FC:1:50-1:100 Reactivity: Human, Mouse, Rat Specificity: This antibody recognizes BUB3 protein. Immunogen: Recombinant protein corresponding to the N-terminus of human Bub3. BUB3 (budding uninhibited by benzimidazoles 3 homolog), also known as BUB3L or hBUB3, is a conserved component of the mitotic spindle assembly complex (MCC). It contains five WD repeat domains and forms cell cycle constitutive complexes with BUB1 and BUBR1. BUB3 is essential for the ki- netochore localization of BUB1 and BUBR1. As a component of the MCC, BUB3 is involved in the essential spindle checkpoint pathway that operates Description: during early embryogenesis. The spindle checkpoint pathway functions to postpone the initiation of anaphase until chromosomes are properly at- tached to the spindle. This acts to ensure accurate chromosome segrega- tion. In addition, BUB3 plays a role in regulating the establishment of cor- rect kinetochore-microtubule attachments. BUB3 is also thought to bind Tctex1L (or DYNLT3), a dynein light chain. Uniprot: O43684(Human) Q9WVA3(Mouse) BiowMW: 37 kDa Buffer: 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. Storage: Store at 4°C short term and -20°C long term. Avoid freeze-thaw cycles. Note: For research use only, not for use in diagnostic procedure. Data: Gene Universal Technology Co.
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