What is wrong here? BIMA82: Developmental Biology and Genetics
Gametogenesis & Fertilization
Stefan Baumgartner Lund University Sept. 11, 2018
What is Developmental Biology? Phylogenetic tree showing the positions of some models organsims used in developmental biology ”Developmental Biology is at the core of all biology. It deals with the process by which genes in the fertilized egg control cell behaviour in the embryo and so determine its pattern, its form, and much of its behaviour.” Wolpert 2015 ”For animals, fungi, and plants, the sole way of getting from egg to adult is by developing an embryo. Whereas most of biology studies adult structure and function, developmental biology finds the study of the transient stages leading up to the adult to be more interesting. Developmental biology studies the initiation and construction of organisms rather than their maintenance.” Gilbert and Barresi 2016
1 Developmental Biology Model Systems Comparative developmental milestones for the six model systems
System Advantages Disadvantages gastrulation segmentation Yeast Very good genetic system. Easy to Unicellular organism. Limited manipulate. Short generation time. cell-cell communication. Limited set of genes. hatching/birth
Very good genetic system. Easy to Simple organism. 959 cells. C. elegans manipulate. Short generation time. Specialized genome. last molt/ metamorphosis Excellent genetic system. Excellent Drosophila tools for manipulation. Short No vertebra. generation time. No genetics. No mutants. Xenopus Vertebrate. Eggs easily accessible. Transparent embryos. Ectopic studies. Limited phenotypic studies.
Zebrafish Vertebrate genetic system. Embryos New system. Genetically not well easily accessible. Transparent embryos. developed. Long generation time.
Chicken Vertebrate. Eggs easily accessible. No genetics. No mutants. Ectopic studies. Limited phenotypic studies.
Mammalian genetic system. Good tools Complex organism. Long generation Mouse for experimental manipulation. time. Embryos inaccessible. Expensive.
Historical aspects I Historical aspects II Early Scientific Period: Zeus Ernst Haeckel 1834-1919 Thomas Hunt Morgan William Harvey 1651 liberating Book: “Generation of “Entwicklungsmechanik” 1866-1945. “ Model systems”: living Drosophila. Nobel price 1933 Animals” beings weak magnifying glasses
Nikolas Hartsoeker future 1695 “Homunculus” structure is already Marcello Malphigi present at 1673 chicken egg 1st stage primitive microscopes of development 1800-1880: Era of “Recapitulation” or Karl von Baer Charles Darwin 1920 - 1950 1945 - 1985: 1985 - : Era of Molecular “Embryological parallelism” Johann W. Goethe Ernst Haeckel Era of Biochemistry Era of Cell Biology Biology and Engineering
2 Concepts in Developmental Biology Germ Layers Axis
Fate Map Developmental Commitment
not committed
committed
3 Cytoplasmic Determinant Induction
Lateral inhibition Stages of development presented in the course
Week 2: Fertilization, cleavage, C. elegans Stefan Baumgartner Week 3: Early Development I: Early development in invertebrates: Drosophila Udo Häcker Week 4: Early Development II: Early development in vertebrates: amphibians & fish Udo Häcker + Labs Week 5: Early Development III: Early development in vertebrates: mammals and birds Udo Häcker + Labs Week 6: Neurogenesis and eye development Per Ekström Week 7: Paraxial mesoderm / Limb development and regeneration Edgar Pera Week 8: Organ development / Influence of the environment on development Edgar Pera, Stefan Baumgartner
2, 8 3, 4, 5 6 7, 8
4 Week 2: Gametogenesis, Fertilization and Cleavage Week 3: Early development in invertebrates: Drosophila
A-P axis formation in the oocyte
A-P axis formation in embryos Segmentation mutants Maternal genes Gap genes Pair-rule genes C. elegans Segment polarity genes Hox genes
gametogenesis fertilization Establishment of D-V-axis
cleavage Gene targeting cloning iPS cells gastrulation
Week 4: Early development of vertebrates: amphibians and fish Week 5: Early development of vertebrates: mammals and birds
Spemann experiment
Formation of the Spemann Organizer Xenopus and axis formation zebrafish
Gastrulation in amphibia and zebrafish Convergent extension PCP pathway Left-right symmetry
5 Integrated in weeks 3-5 : signalling events during early development Week 6: Neurogenesis and eye development Wg (Wnt) Birth and migration signalling of neurons: pathway Stratification Hedgehog Neural crest cells signaling pathway
growth cones trophic factors
Shh and Bmp in neural patterning
RTK-signaling pathway Sevenless pathway Notch pathway Hippo pathway Neural induction eye development, eye field specification Cyclopia due to defective Shh signaling TGF β pathway Neurulation Formation of lens, cornea, retina
Week 7: Paraxial mesoderm / Limb development and regeneration Week 8: Organ development & Influence of the environment on development
temperature and Contergan (Neurosedyn) sex determination
salamander planaria
Organs: Bisphenol (BPA) • (endoderm) • pancreas alcoholism hydra • kidney
Epimorphosis: adult structures dedifferentiate, grow and respecify into new structures apical ectodermal ridge AER, zone of polarizing activity ZPA Morphallaxis: existing structures repatterns, little regrowth Zika virus
6 relative sizes of eggs Structure of the human egg
Stages of oogenesis Structure of the sea urchin egg from first menstruation onwards !
3-8 months of embryonic ~ 400-800 000 growth oocytes
7 Maturation of the follicle. The hormonal cycle of women. oviduct = fallopian tubules LH
FSH
Pregnancy test: mab against hCG (human Chorionic Gonadotropin, 244 aa) estrogen progesterone
at birth Spermatogenesis Stages of spermatogenesis at puberty:
primary Meiosis spermatocytes
during secondary spermatocytes ca. 24d embryogenesis
birth spermatogonia
ca. 35d