Nat His t. Res.. Vo l. 9 No.1: 29 - 39. March 2006

Fungal Flora in Chiba Pref., Central Japan (V). Gasteromycetes 2. Additions Additions to the Family Lycoperdaceae

Taiga Kasuya 1) and Ken Katumoto 2)

11 11 Laboratory of Plant Parasitic . College College of Agrobiological Resources. University of Tsukuba Tennodai. Tennodai. TSllkuba 305-8572. ]apan E-mail: E-mail: [email protected] 2055.27. ~) 2055.27. Ouchi -o hori. Yamaguchi-shi. Yamaguchi 753-0214. ]apan

Abstract Abstract Eight species of Lycoperdaceae (Gastero口1Y cetes) collect巴 d in Chiba Pr巴 fectllre. a ceI日1tral area of ]apan ]apan are report民ed仕:Cα ! l"(叫E

ni句gl陀'es町ce叩Il ιS ， L 、仇， hiυmη10US店Eαt川川11111山4υ1m and L. lllllbrilllllll. Among them. L. shill /O llSa l11 lm is a new species and D. calldida is a new record for ]apan. C. bOllillellsis, C. cralliijimllis, C. rllgosa, C. llrrij�mis and L. 11mbrilllllll are newly recorded

for for Chiba Prefecture. Alltoflllorescense of basidiospores and capillitia of 4 Cll!l'arill , Discisedll and Lycoperdoll species species are described by the flllorescence microscopic observation. Observations of their alltoflllorescense sllggest sllggest their significance for morphological c1 assification of Lycoperdaceae.

Key words: alltoflllorescence. Chiba Prefectllre, Lycoperdaceae, new records, new species. .

This This paper is the second in a series of taxonomic Materials Materials and Methods studies studies on the family Lycoperdaceae in the herbarium of of Natural History Museum and 1nstitute, Chiba All the materials examined were deposited in the herｭ (CBM) , Chiba Prefecture, ]apan. The first paper barium of the Natural History Museum and 1nstitute, (Kasuya, 2004a) discussed the 12 species of this famｭ Chiba (CBM). Macroscopic characters were described ily. ily. 1n this paper, 8 species of the genera Cμ /varia ， by the observations on dried materials. For visible Disciseda Disciseda and Lycoperdoll were recognized. Among light microscopic observations, free-hand sections of them, 1 species of Lycoperdoll is a new species and 1 gleba and peridium were mounted in water, 5% KOH species species of Disciseda are newly recorded from ] apan. (w /v) , and 1 % cotton-blue lactophenol on glass slides. Furthermore, 5 species of Ca/vllria and Lycoperdo l1 are Forty or 50 randomly selected basidiospores were newly recorded from Chiba Prefecture. measured for each specimen under a light microscope. 1n 1n lycoperdaceous fungi, autofluor 巴 scence specific to For scanning electron microscope (SEM) observaｭ their their basidiospores and capillitia is frequently obｭ tions of the basidiospores of L. shi Il1 011Sall11 1l1 Kasuya & served served (Kasuya, 2004b). Fluorescence can be obｭ Katum.. a small portion of gleba was coated platinum served served easily by means of excitation with blue-violet palladium in an ion sputter (Hitachi E-1030: Hitachi, radiation. radiation. 1n many cases. autofluorescence of organｭ Tokyo, ]apan) , and observed under a SEM (Hitachi S- isms isms shows a characteristic pattern which is different 800) operating at 15.0 kV. For the observations of the from the image observed under visible light (Ando er autofluorescence of basidiospores and capillitia, small al., 1991). Th巴 refore ， the detailed 日 tructure of portions of gleba of Cll/valia cralliiformis (Schwein.) Fr .. basidiospores basidiospores and capillitia of lycoperdac 巴 ous fungi, Disciseda cα Il dida (Schwein.) Lloyd, Lycoperdoll which are difficult to recognize under visible ligh t. 川 grescells Wahlenb. ・ Pers. and L. shi ll1 0usanum were may be described much more dearly using the fluoresｭ mounted in lactopheno l. and were observed by a Zeiss cence cence microscope. This paper describes Axiophot microscope equipped with an Epiｭ autofluorescense autofluorescense � basidiospores and capillitia of 4 fluorescence system. Zeiss blue-violet filter (05 filter) Ca/varia, Discisedαand， Lycoperdoll species by the fluoｭ was selected as an excitation filter: this filter has transｭ rescence 口 lICroSCOpy. mission at 395-440 nm.

- 29 Ta i ga Kas u ya and K 巴 n Kat um oto

200 5) ancl Th ail ancl (R uk sawo ng an d F I 巴 ge l. 20 01 ) Taxonom y Spec im ens exa min ed: C hib aP ref.. 1ml>a-gun. Sak aeｭ Cal叩 tia bOlliuellsis S.1t o & 1m ai. T rans. Sapporo N at. m ac hi .R yuk akuji. Jul y 28 .1 99 1. co l1 .Ch iba Myco l. C lub Hi st. Soc . 16: 9. 193 9 s.I1. . C B M -FB -3199 : C hib a P ref.. C hib a-shi .C hu o-ku . Fi g.1 A oba- cho. Se pt em ber1 8.1 99 1. co ll. T. F ukih a ru S .I1 ..

Bas idiom ata large . sub glob ose to t urbin a t巴 . 5 0-160 C BM- FB -3 308: sa m e pl ace. A u gust 17. 1993. co ll. T mm hi gh. 3 0-170 mm br o ad. ye ll ow ish br ow n to da rk Fukih a ru s. n.. CBM -FB -90 2l: sa m e pl ace .JU I1 巴 29. 1 9 94 . br ow n. w ith thick w hi te rhi zomo rph s up to 40 mm . coll. R. Onum a s .n.. CBルトFB- 111 94: same pl ace . Se pｭ Exo pe ridium mad e of ve ry t hin. papery. almost te mb er 24 . 1994. co ll. T . Fu kih a ru s.n. . CBM -FB- 1l 5 53: sm oot h sca les . ye ll ow ish b row n w hen yo un g. later sa m e pl ace .Jun e 25 . 1998 . co ll. Y. Abe s.n .. CBM -F B - bl ac ki sh br ow n. s lou ghin g 0任 at m atun ty 16529:sa m e pla ce. ]ul y 7. 1998 . co ll. T .F ukih aru s .n E nd o p 巴 ridium thin .s hin y. ye ll ow ish br own to da rk C B lvl -F B-1 666 0: sa m e pl ace. Jun e 30. 20 00 . co ll. Y br ow n. late r br eakin g away by deg r ees in frag m en ts K oya m a s .n.. CBM -FB -24678: C hib a Pre f.. C hi ba-s hi. G leba pul ve rul ent. w hi te w hen yo un g. late r In age -ku. ln ag巴ー hi gash i . ]ul y 7 .1 993 . co ll. F .K os hin o oc hr aceo us. S ub gleba we ll deve loped. s pongy . s. n.. CBM -FB -560 8: C hib a Pre f.. C hib a-shi . M id o ri-!王 u. oc hr aceo us to da rk bro w n ~o r o-c h o . ] ul y 2 1. 1986 . co ll. Y. I-I or i 巴 s. n.. CBM-FB - Bas idi os p o r es 巴 llip so id to cy lindri c. r a r 巴 I y ovo id . m ト 1902: sa m 巴 pl ace. Se p temb 巴 r 17. 1994 . co l1. C hib a nute ly ve l・ ru cos 巴. (3.0- ) 3.5-5 .0 ( ・5.5) μm in cli a m 巴 te r. Myc ol. C lub s.n.. CBル1- FB - 1l 46 l: C hi ba Pre f.. C hib a- shi w ith a hya lin e pedi ce l up to 1. 5 μm long. Bas icli a n ot M iham a-ku . Ma kuh ari-ni shi . Octo ber . 2. 1996. co ll. T obse r ve cl. Ca pillitium Lyco perdo n- ty pe. 2.0- 4. 0 (-5.0 ) Wa da s. n.. CBM -FB- 15233 : Ch ib a Pr ef.. C hose i- gun . μm broad. rar ely bran ched. br ow n to cl a rk br ow n. Ic hinomi ya -m ac hi. To ram i. Octo ber 27 . 1987, co ll. F S 巴 p tate. por巴 s sm all. irr eg ular. wa ll s 0. 5・1. 0μm thi ck. K oshin o s .n. . CB .tI II-FB-246 05; C hib a Pre f.. Awa-g un. n ot pitt ed. Para capilli tium rarely pr ese nt. Tomiur a-m ac hi. Ta racl a. Se ptem b er 20. 2000 . co l1. T H abi ta t: O n ri ch so il or humu s in woo cll ancl. Kaw an a s .n.. CBM-FB-24838 Di stribu tion :]ap an (1m aze ki e l a/.. 1 988: It o. 19 59 :lt o japan ese nam e: O u ー n o u ta k e. and 1 mai. 19 3 9). 1ncl ones ia (K as uya and R et nowat i. R 巴 m a rk s: T hi ss pec i es is new ly I 巴 p orte d here fro m C hiba P r 巴 fect ur e. T hi ss pec i es is m orph olog ica ll y s im ト

lar t o C. cra fo nii /"l ni s (Sc hwe in. ) F r.. bu t t he bas idi os por es of th ese two spec i es are quite cl iffe r巴 I1 t C a/ vati a bo nin ensis h as ell ip so id to cy lindri c bas idi oclp ores . bu tt h ose of C. crani ifo rm is are glob ose

to to s ub g l o b os巴 Ca / va t ia bon ine nsis was ori gin all y cl eｭ sc rib ecl from B onin1 slancl s. So uth eastern Ja pan (1 to and 1mai, 1939) . late r al so co ll ecte cl fro m tropi ca l. subｭ tropi ca l to wa rm -te m pe r ate a r 巴 as of Easte rn As ia (Im aze ki et al. . 1 988: 1to .1 959 : Kas uya ancl R etn owa ti. 2 005: Ruk sawo nga ncl F lege l. 200 1).

Calvatl・ a c /"O lliiformis (Sc h w 巴 in . ) F r.. SUl11 m a Veg Sca ncl.: 442. 1849 F ig.2

Bas idi om ata large . sut>globose to tu rbi nate . 40 -1 20 ~ 111m 111m hi gh4. 0-2 00 m m br oad. ye ll ow ish br own to re dｭ ~ di sh b row n. w ith thi ck w hi te rhi zomorp hs up to 3 0 4Z22霞ミミ mm. Exo p ericlium m acl e of ve ry thi n. pa pery. sm oot h C to m 日1ut e l y fur furaceo us sca les. pa le och r aceous w hen yo un g.l ate r cl a rk br ow n. slo ughin g 0妊 at m at unt y 語耳~ E ncl op ericlium thi n. shin y. ye ll ow ish brow n to reddi sh 匙~が: brown, late r br ea ki ng away by cl 巴 grees in fr agm 巴 n ts ri g. 1. Ca{val ia bO l1 il1 el1 sis S .1to & lm ai (CB �I -F13- J5233 l G leba pul ve rul ent. w hi te w h 巴 n yo un g. later f¥. f¥. 13as idiom a: 13 . 13as idi os po res: C. Ca pillit ia. a. ca pilli tial thr eacl: b.d eta il of capilli tium. Sca le ba r s f¥: JOm m. B:3 .5 oc hr aceo us to br own . Subg l 巴 b a we ll cl eve loped. μ111. Ca :20μ111 . C b: 10μ111 spo ngy .oc hr ac 巴 o u s to brow n

qハ Ju A clcl it ions to Lycop erdacea e (Gastero m ycetes ) of Chiba j. apan

D ist 1' ib uti on: Com m on in tem per ate to wa rm te mp er ate ar eas in Japa n. A l so kn ow n fro m C hin a (L iu . 1 984; Fa n el al., 1994 ). In d o n 巴 s i a (Kas uya and Retnowati, 2005) , Ca nada (Co ker and Co u ch. 1 928 ) 。 U ni te d States (Co ker and Co uch. 1928 : Ze ll er and Smi th, 19 64) and Mex i co (U ri sta el a/. , 1985 ) Spec im ens exa min ed: C hib a P ref.. A bik o -shi. Nakabyo, November , 1. 1992, co ll. Y. K ud o s.n., CBlv lｭ FB -4 847; C hib a Pr ef.. Imb a-gun . Sa kae -mac hi Ry ukakuji, O ctober 6, 1991. co ll. Y .H o ri 巴 s. n.. CBM-FB 6058 : C hi ba PI 巴仁 Sa ku ra - s hi ， Sa kur a. Octo ber 4. 1997 . co ll. C hib a Myco l. C lub s.n. .CBM -FB -1 6073 :C hib a P re f.. C hib a -shi. C hu o ー ku . Ao ba-cho. ]u ne 11. 1998 . co ll. Y A be s. n" CB M - FB ・ 1 6468 : C hib a P ref.. Chiba-shi, C hu oｭ ku. Inohana, Jul y 26.1986, co l1. Y. H ori es .n" CBM -FB - 19 07: C hi ba P ref.. C hib a-shi . M id or i-ku. No ro-c ho. Se ptembe r 26, 1994 .co ll. R. O num a s.n " C Bl vl- FB -1l 630: C hib a Pre f.. C hi ba -shi. Waka ba -ku. Tabeta-cho, ]un e 16 .

1 998. co ll. K. Ots u ta s.n" CBM -FB -1 6513 : C hib aP 1' ef.. P b 三二三 K imi ts u- sh i. Oza ki. Octo be 1' 2, 1992 . co ll. C hib a Myco l. C lub s.n.. CB.M-FB -4 509: C hib a P 1' ef.. Kamogawa -shi . Izu m i. Octo ber 22 .1 987. co ll. F . Kos hin o s.n.. CB l¥Il -F B- 。 辻zi 7 06 ]apan ese na m e: No u ta k 巴. R em arks : T hi ss pec i es is new ly I 巴 p o r ted h e r巴 fr o m C hib a P r 巴fect ur e. Ca / va tiα cFO niif'o rmis is the most co mｭ mo n] apan ese spec i es of Ca[vcil ia. S ur face orn am en ta

tion an dp edi ce ls of bas idi ospores of C. C I ω li i!'o rl1l i s ca n beo bse rve d mo re cl ea rl y und er fl u orescense m icr os copy than visibl e li ght.

Ca/ l' atia rugo sa (Ber k. & JVl . A. C ur tis) Reid . Kew B ull 31:6 71. 1976 . Fig .3 Bas idi om ata su bglob ose to de p ressed g lobose. 20-45

Fig .2. Ca lvor ia c ra }'/i ifo n 川s (Sc hwe in. ) Fr (CﾟM -Fﾟ -7 06 ) mm hi gh. 30-100 mm br oa d.p ale oc h1' aceo us to reddi sh A . ﾟas id ioma: ﾟ. Basidiospores: C. Capi lli tia. a. cap ill itia l br ow n. bu t turnin g ye ll ow w h巴 n brui se db efore m at urｭ t hread: b. deta il of ca pilli ti um: D. Autofl uo rescence of ity . w ith thick, w hi te rhi zo m orphs up to 50 mm long bas idiospo res a nd capilli tia. Scale ba rs A: 7 m m. B: 4.0μ Ill. Exo per idium ma de of v 巴 r y thin . furf ur aceo us spin es . Ca: Ca: 20 ,U m . Cb: 10μm . D: 13μ m pale ye ll ow ish br ow n to oc h raceous w hen yo un g. late l

Bas icli os por es glob ose to su bglobose . m inu te ly da rk br ow n.s lough in go ff at matu ri ty . En d op 巴 r idium verrucos 巴. (3 .0 -) 3.5-4 .5 ( -5.0 ) μ m in cli amete r exclucl t h 川。 s hin y ， ye ll ow ish br ow n to red di sh br ow n at upp er ing orname n ts or (3 .5-) 4.0-5 .0 ( ・ 5 . 5 ) μm in diamete r p arl. w lli te to crea m at b ase. l ater break ing away by in cludin go rn aments. w ith a hya li n 巴 p e di c 巴 1 up to 3.0 d 巴 g r ees in fragments . G leba pul ve rul 巴 n t. w hi te w h 巴 n

μm lon g: wa ll s. orn am en ts and p 巴 d i ce l s of bas idioｭ yo ung .l ate 1' oc hr aceo us to brow n. Sub gleba mi nute ly spo r es flu oresce . Bas idia not obse 1' ved. Cap illi tium deve loped 0 1' so m et im es abse nt. com p ac t. pale ye ll ow

Lycope rdo ll- type. 2.0- 6.0 ( ー70 ) μ m b1' oad . oc 仁 asiona ll y to oc hr aceo us dichoto m ous ly bl-anched. ye ll owis h brown to da rk Bas idi os por es g lobose. minu tely ve 1' 1' u cose. wa r ts up b row 口. septat 巴. po r es sma !1. ir reg ul ar. abun da nt. wa ll s to 0 . 5μ m high, ( 3 . 0 ー ) 3 . 5 - 4 . 5μm ind iam ete r excl udin g 0 .5 ・ 1. 5μm th ick. not pi tte d. wa ll s and s 巴 pta fl u oresce. orn amen ts or (3.5- ) 4. 0 ・ 5 .0 μm in di am ete r includin g Pa 1' acapi lli tium abs 巴 n t. orn am ents p. ale ye ll ow ish br ow n to ochraceous, w ith a

Hab i ta t: On ri ch soi l 0 1' humus in woo cll a ncl hya lin e p 巴 d i ce l ， 1. 0- 1. 5μ m lo ng. Bas idia not obse rve d

勺、'EE υ A Taiga Taiga Kasuya and Ken Katumoto

similar similar to C candida (Ro st k. ) Holl� s. Furthermore. ¥ ]apanese m ate rials of C. ca ndida were also coll 巴 cted on A the dead trunk of deciduous trees (Kasuya . 2005) 。 Howe ve r. C. candidαis easily separated from C. ru gosa byw hitish basidioma. Thes e morphologic al and eco ｭ logica l characteres of c. ca ndida suggest th 巴 close rel a tionships tionships between C. ru gosa and C. cCl ndida. P e ridium morpholog y of this species is similar to C. craniiformis, but but C. cran 日iforl11 is has mor e deve lop ed subgl 巴 ba than

c.川gosa

Ca lvatia lI /riformis (Bul l. P ers .) ]aap, Ve rh . Bot Vereins Vereins Pro v.Brandenburg 59: 37.1918. Fig.4. Fig.4. Basidiomata Basidiomata large . globose to pyr iform. 45-60 mm high. high. 40-60 mm broad. cream to yellowi sh brown . with white white rhizo l11 orphs up to 20 111111. Exoperidiu l11 m ade of th ick pol yg onal plates. furfuraceous spin es pr ese nt al11 0ng the plates, white when young. and then crea l11 to to yell owish brown. later sloughing 0任 Endoperidium thin. thin. paper y. shiny. white to greyish brown. fragile on Fig.3. Fig.3. Cα IV{/I;a rugo.凶' ( Berk. & M .A .Curtis ) Reid (CBMｭ the upper part w h巴 n maturit y. Gleba pul verul ent. FB-14411 ). A. Basidiomata: B. Basidiospores: C. Ca pillitia. a white white when young. later yellowish bro w n. Subgleba pillitial ca pillitial thread: b. deta il of capillitiu m. Scale bars A : 10 mm. B: 4 .0μm. Ca :20μm. Cb: 10μm well developed, spongy, white to ochaceous Basidiospores Basidiospores globos巴， al l11 0st smooth. 3 .5 - 4.5μm lI1 Capillitium Capillitium Lycop e l τio n- ty pe. 2.5-4.5 μm broad. occaｭ diameter . with or without a shor t hya lin e pedic el up to siona l1 y br anch ed. y 巴 llowi s h brown. se ptat e. often 0.5μm long. Basidia not observed. Capillitium br ea king into shor t particl es at se ptations . pores abunｭ d ant. sma!!. wa lls ve ry thin. 0 . 5- 1. 0μm thic k. pitted Paracapi l1 itium absen t. Habi ta t: On the dea d trunk of deciduous trees. espeｭ cia l1 y Fagaceae. Di st ribution :]ap an. E urop e (Sarasini. 2005 ). U nited States States (Co ker and Couch . 192 8). Central America (R eid . 1976 ).South A m er ica (Rick. 1930 ) and Oceania (Cunningham. 4194 ). Specimen exa min ed: Chiba Pr 巴f.. 1chihara・shi. Tsukizaki. Tsukizaki. October 21. 1995 . co ll. Chiba. Myco l. Club n.. s.n.. CBM-FB-14411 ]apan ese nam e: 1ro gawar i-hokoritake Remarks: This spec i es is new ly report ed her e from C hib a Prefecture. Previously. thi s s pec ie s was reｭ ported ported as C. Iï伽 ojlava (Cragin ) Ll oyd in ]apan (Yoshimi and Hongo. 1989) . Ho weve r. C 川 brojla νa is considered considered a synonym of C. ru gosa because both ha ve yellowi sh to r e ddish 巴 ndoperidium . small subgleba and globo se basidiospor es (Reid. 1976: Sarasini. 2005 ). 1n C hiba Prefecture. this speci 巴 s so m et ime s occurs on the dead trunk of deciduou s trees. especially on Fagacea 巴， Fig. Fig. 4. Ca!vatia IItri{or ll1 is (Bul l. : Pe rs. ) Jaap (CBM ・ FB- a lthough there w as no pr 巴 vious r 巴 port of this subｭ 5595 ). A. Bas idiom ata : B. Bas idio spores : C. Cap illitia. a. st rat e as a habit at of this species. Basidioma morpholｭ capillitial thread: b. d eta il of capi lli tium. Sca le bars A: 15 ogy and mi cro sco pi c c haracte r 巴 rs of this species ar 巴 mm. B:4.0μm ， Ca: 20μ m. C b: 10μm

32 32 Additio ns to Lycop e rdaceae ( Gasteromyc 巴 tes ) of C hiba. ]apa n

Lycoperdon-type, 2.5-11.0 μm broad, di chotomo usly branched, ye llowi sh brown, aseptate, pores sli t-lik e or ^ irregular, abundant, walls 0.5-2.0 μm thick, pitted 町・ Paracapillitium Paracapillitium abse nt. Exoperidial pla tes made of hya ｭ line, subglob ose sp hae ro cys ts up to 3 0-50μm in di- ameter Habi ta t: On sa nd y so il among grass Distributi on: ] apan, E urop 巴 ( Kr e is e l， 19 89 ; Lange, 1990) , South Af ric a (Bottomley, 1948) , Kazakhstan (S hva rcman and Filimonova, 1970) , China (Liu, 1984) , Himala ya (Ahmad, 1941; Kr 巴 is巴1， 1989) , Mo ngo li a (D�f elt and Bumzaa. 1986) , Canada (Co ker an d Couch. Couch. 1928) , U nit ed States (Co ker and Co u ch. 1928 : ーハ》 Zeller Zeller and , 1964 ) and Mex i co (U ri sta el al " Fqu Smith 19 85 ) Specimen exam in ed: C hib a PreL C hiba- shi. M ih ama ｭ ku, Takahama. co ll. F. Koshino s.n" CBM -FB-5595 Hu-­ Jap anese name: Kikumetake 。 Remark s: This spec i es is new ly r 巴 po rt ed h ere fro l11 Chiba Chiba Prefecture. p ，巴 viously. thi s spec i es was reｭ port ed as C. cae la la (B ul l. ) Morg an in ]apan (Yos himi and Hongo, 1989 ).However. C. cae lara is co n s id 巴 r 巴 d a synonym of C. utrif o nnis b ecause bo th ha ve t h 巴 same structures structures of peridium and simi lar morph ology of bas idi ospores and ca pillitial pores (Kreise l. 19 89 ). This sp 巴 cies is di st in gui she d from oth巴 r J apanese spec i 巴 s of Cal νaria by t h e 巴 xope ridi a l polygonal pl at巴 s ， and the pre sence of sli t- lik e pores in cap illi tium . Ca!va li a excipu lif onnis (Pe rs. : Pers. ) Perdeck also h as sLi t-Lik e ca pilliti al pores, bu t th iss pec ie s isc lear ly s 巴 parate d from C. llll 拘 rmis by the high st ipitum bas idiom ata . and prominently wa rt ed ba sidio spor es . Ca! νどllW utriformi s h as one va ri ety. i ι C. utri f' onni s var Fig . 5. D isciseda calld id a (Sc hwein. ) Ll oyd (CBM -Fﾟ hungarica hungarica ( Holl�) F. Smarda. It is morphologicaUy 35 143 ). A. ﾟa sid iomata: B. Bas idi ospor es : C. Ca pi lli tia. a. simil ar to the vari ety ulriformis except it s rema rk abl y capillitial thr ead :b . d eta il of capi lli tium: D. A u to 日 u o­ depr esse d b as idi omata and no t developed subg l eba resc enc e of ba sidiospores and capillitia. Sca le bars A :7 mm .B: 5.0μm. Ca: 20μm . Cb: 10μm. D : 1 5μm (Calonge, 1998: Martin. 1997 )

Disciseda Disciseda candida (Sc hwe in. ) Lloyd, Myco l. N otes ve rru cose. warts up to 0 .5 μm high, 4 .0 -5 .0μm bro ad 1 (1 0): 100, 1902. ex cludin g ornaments or 5.0-6.0μm bro ad includin g or Fig .5 nam e nts. w ith as h ort. hya lin e pedi ce l up to 0.5μm

Ba sidiom ata in s mall gro up s 0 1' so li ta ry. hypogaeo us long, ye ll ow ish to oliv aceo us bro w n: wa ll s. ornaments w hen you ng. late r subh ypoga eous 0 1'叩 1 9aeous. and pedic els of basidiospores fluoresce. Basidia no to bｭ globose globose to depr esse d glob ose. 10- 20 mm br oad. wh ite se r ved. Cap illi tium Lyco perd on - type. 3 .0- 6 . 0 μm br oa d. to to w hi tis h gr 巴 y. Exoper idium thin .bri tt le. brown. fa ll en fi lam e ntous. r a r巴 l y branched, septat e. breaking int o away except for the basal ar ea leav in ga c up -shaped shor t particles at septat ion s. por es abund an t. ye ll ow i sh remaining remaining at m atur it y. encru ste d w ith sand br ow n. wa ll s 1. 0 μm thi ck. not pitted. strong ly fluoｭ

Endoperidium w hi t巴 to w hiti sh g rey. papery. resce. Paracap illitiu 口1 absen t. pulverulent, tough, op巴 ning w ith a sma ll api ca l pore up H abi ta t: On dr y.sa nd y so il among grass n ear the sea to to 4 mm wide. G le ba wh ite w hen young. later shor e. oli vaceo us brown, powdery. Subgleba abse nt. Di st ribu tion : ]apan.E ur ope (Ca lon ge. 199 8: Kers, Basidiospores Basidiospores glo bose. almost smoot h or minutel y 197 5: Mo ravec. 1954 ).So uth Af ri ca (Bottomley, 194 8).

ηtu内ぺ υ Ta i ga Kasuya a nd K en Ka tu moto

U ni ted S tates (Co ker and Co uch . 1928 ). Arge ntin a (Mo r avec. 195 4) and Ocea ni a (C unn ing h am. 1944: G rgurino vic. 9197 )

Sp 巴 c imen s ex amin ed: C hi ba P r 巴f.. Sou sa - gun 守 N osa ka-m ac hi . Notehama, D ece mb er 21, 2003, col 1. H. Sa kam oto s.n., C BM-F B -351 43 :C hib a Pref., Sambu-gun, Hasunuma-mura, Min amih am a. D ece mb er 21, 2003, co ll. 1. Asa i s.n., CBM-F B -35 142: same place, Fe bru ary 25, 2004, co l1. 1. As ai s. n.. CBM -FB-35 144. ]apan ese nam e: Us uir o-d onguri ta ke (new ly nam ed). R em ark s:Th e ge nu s Di sc i seda co mpri ses 19 spec i es and di st ribut ed in the , sa nd y envi ro w id 巴 I y arid nme nt Fi g. 6. AutoO uo rescence of basid iospo res and capill itia of of of th e wo rld (G rgurinovic, 1997 : Kre ise 1. 1976 : Lyco l' erdo l1 l1 igresce l1 s Wa hl en b. : Pers (CﾟM-Fﾟ-16308 J. Mo r avec. 1954 ). Th e r 巴 a r e 2 s pec i es of Di sc ise da Sca le ba r:J 5 JI m kn ow n to have alm ost sm oo th to minu te ly v 巴 rru cose bas idio spores : D . all o5 lrali s G. I-1. C unn. and D. ce l ν lnQ Oujinb o-c ho. Octo ber 14. 2000, col 1. C hib a Myco 1. C lub (Be rk. ) H oll � (C unningham, 1944 :G rgurinovic, 1 997) s. n.. CBM-FB-3 00 38 : C hib a Pr ef.. C hib a-shi . C hu o・ ku ， However, D. ca ndida is cl ea rl y di st in gui shed from bo th Ao ba-c ho. Decem ber 18, 19 97 . co l1. Y. A be s.n .. CBMｭ D. D. all slr ali s and D . ce rvina by its more w hiti sh FB-163 08:C hib a Pref.. 1chih ar a-shi, Shimono, Octo bel end operidiu m a nd n ot co nspi cuo us. sm all api ca l po re. 27. 1993. co l1. C hib a Iv Jyco 1. C lub s. n.. CBM -FB-9779 A n ot h 巴 r 2 spec i es of Di sc ise da are prev iously deｭ ]apan ese nam e: K ur o-ho ko rit ake. sc rib ed to have w hi tis h end ope ridium an d sm all api ca l Rema rk s: Sur face orn am e ntation and short p 巴 di ce l s pore Ii ke D. ca ndida : D . bov isla (K lotsc h) H enn . nda D . of bas idi ospo r es of thi s spec i es ca nn ot be r巴 cog ni ze d sll blerran ea (P ec k) Co k er & Co uch (Kers, 1975; und er f1 uoresce n ce microscopy, bu t unde r visibl e lig ht. M it chel el al., 1 975). However, D . bov isla is eas il y se paｭ th ese are cl 巴 a rt y observe d. ra te d from D . candida by it s large . ve rru cose ba sidi os por es (6 .0-9.0μm broad, K 巴 r s. 1975 ).Di sc ise da Lyco perd oll sllilll Oll o5 a l/ ll 1I/ K as uya & K at um .. sp. nov sllbl erran ea is al so di sti ngui shed fr om D . ca lldi da by it s F igs.7 -8 m ore sm all er bas idi om ata up to 7-1 5 mm br oa d and Basi di om ata in cate r va par va form a ntia ve l gregaria, m ore spinulo se ba sidi ospo r es (M itc hel el a l川 1 975) . 20- 65 m m la ta. 35-75 111m alta, turbi nato- pirif ormi a. Mo rph olog ica l charac ter es of the ]apan ese spec im ens c r 巴 m ea ve l oc hr acea. Ex op e ridium pri mo albidu m ve l are we ll id entic al w ith th e desc rip tions of D. calldid a crea m eum . deind e pa llid e brunn escens v巴 1 fuscoｭ (C unnin gham . 19 44: Grgurinovic, 1997 : Kers, 197 5; brunn esce ns. spi nis frag ilibu s co ni cis ve ls ub co ni cis 1-2 Moravec, 19 54) . and th ose ] a p anes巴 m ate ri a l s are m m longis d e niqu 巴 sece d e n ti bu s inte rdu m gran uli s id entifi ed as D . cα I! dida . T hi s spec i es is new ly reporte d c r巴 m e i s pulver ul entib co us mmi xti s obtect u m. 巴X here fro m ]ap an. ce Ll uli s globos is ve l sub globos is 5. 0 -7. 0 μ m di am Surf ace orn am entat ion and pedice ls of bas idi os por es hya lini s co mp os itum. E nd ope ridium papyraceum , of of D. ca ndi da ca n b e o b se r v 巴 d 111 0re clea rl y und er crem eu l11 ve l oc hrace ul11 . lu c idum. ordin at ione ret iculi f1 uoresce n se mi cro sco py than visibl e li ght. As a res ul t. in str uct um . G l eba ju ve nili s alb a. dei n oc hr acea ve l f1 uoresce n ce mi crosc op y is poss ibly use ful to obse rva ｭ brunn ea .p ul ve rul enta .割問 p se u docolum e ll a. S u t沼 l e b a tions on bas idi os por es of Di sc ise da. T h e r efo r e. 自 uores ­ b 巴 n e cresce ns. ins igni te r m ag na. u sque ad 60μm ce n ce mi crosco py sho uld be appl ie d to mo rph olog ica l crassa et 40μm alta. s up e dヨ cie gyrosa ve l spo ngi osa cl ass ifi catio n of thi s ge nu s.111 ]apa n. onl y 1 spec i es of ve l a l v 巴 o l ata . cremea v巴 1 och racea. Bas idi os por ae t his ge nu s. D . s ubl erra nea was hi t h 巴 r to kn ow n glob osae ve l s ub g l o b osa 巴 . epi spo rio 4.0-5 . 0μ 111 di am (Kasuya, 200 4a : Y os himi and H on go. 1989). B ot h D (ve rru cul o 0 . 5μm a lto in cl u so 4 .5 ・ 5.5μ m di am ). ca lldid a and D. o5 ubl erra ll ea are freq u 巴 n tly co ll 巴 cte d oli vaceo-brunneae, sin e pedi ce ll o: p ari es cum fr om coasta l sa nd dun 巴 s in ]ap an ve rru culi s flu oresce ns. B as idi a non obse rva ta Ca pilli tiu l11 fl avo- brunn eum ve l o li vaceo・ brunn e um. Lycoperdoll Lycoperdoll Il igrescells Wa hl enb . : Pe rs.. Sy n ops. Met h 3 .0 -8 . 5 μm crass um . elas ti cum. as巴 p tat um. in ter dum F un g. : 46. 1 18 01 di chotom i ce ramos um . p ori s non abund antibus F ig.6 praed itu m: pari ete 1.0・ 1. 5 μm crasso non foveo lato 3- Sp ec im ens exam in ed: C hi ba Pr ef.. F un abas hi -s hi . st ratoso. Pa racapi lli tiu m abs巴 n s

q A υ、 ｭ Additions Additions to Lycop巴 rdaceae (Gasteromycetes) of Chiba. ]日 pan

Fig.7. Fig.7. Lwυ'pe ，.c/o l1 shilllOllS (ll1l1l1l Kasuya & Kalum. (CB 1v !-F B- 3250. ho!olype). A. Basidiomata. a. surface of basidioma: b. b. section of baお idioma: 13. 13asicliospores: C. Capillilia. a. capillitial thread: b. detail of capillilium: D. Sphaerocysls of exoperidiu l1l. Scale bars A: 13 mm. 13: 4.5μm. Ca: 20μm. Cb: 10μrn. D: 5.0μm.

Holotypus: Holotypus: CBM-FB-3250. ad terram in sylva. pulverulent. without pseudocolumella. Subgleba very Ryukakuji. Ryukakuji. Sakae-machi. Imba-gun. Chiba Pref. in well developed. remarkably large. up to 60 mm broad. ]aponia. ]aponia. 6 October 199 1. a "Chiba Mycological Club" 40 mm high. gyrose. surface spongy to alveolate. leg.. leg.. in Herbario fungorum Musei et Instituti Historiae cream to ochraceous. Naturalis Naturalis Chibaensis (CBM) cons 巴 rvatus 目 Basidiospores globose to subglobose. verrucose. Etymology: Etymology: "shimousa" means the old name of colｭ warts up to 0.5μm high. 4.0-5.0μ111 broad excluding lected lected place of the type specimen. ornament 日 0 1' 4 目 5-5.5μm broad including ornaments. Basidiomata Basidiomata in small groups 0 1' gregarious. 20-65 olivaceous brown. pedicel lackin g; walls and ornaｭ mm broad. 35-75 mm high. turbinate to pyriform. ments of basidiospores fluoresce. Basidia not observed. cream to ochraceus. Exoperidium white to cream Capillitium Lycoperdoll-type. yellowish brown to when young. later becoming pale brown to blakish olivaceous brown. 3.0-8.5μm broad. elastic. pores not brown. made U jJ of fragile. subconical to conical spines abundan t. aseptate. sometimes dichotomously 1-2 1-2 mm later long. sloughing off. often mixed with branched. Walls of capillitium 1. 0・1. 5μm thick. not jJ it cream pulverulent granules. Endoperidium papery. ted. consist of 3-1ayer: outer layer thin. 0.1 -0.3μm cream to ochraceous. shiny. showing reticulate pattern thick. middle layer 0 .5-1. 0μm thick. and inner layer after after exdoperidiu ll1 sloughing of f. Gleba white when 0 .2 -0.5μm thick. only outer and inner layers fluoresce. young. young. later ochraceous to olivaceous brown. Paracapillitium absen t. Exoperidium composed of

qJF-1υ Taiga Taiga Kas u ya and Ken Katumoto

(new ly nam ed). Remarks: This spec i es is characterized by the pres en ce of spongy to alveolate, we ll developed, large subgleba, non-pedi cellate, verr u cose basidiospores an d thick thick ( 1. 0- 1. 5μm ) . 3-1 aye red walls of cap illitium Exoperidium mix 巴 d w ith conical spin es and pulverulent pulverulent granules. and reticulate endope ridium of

gleba gleba pl ace L. shim o!l sa n. u llZ at Stirps 'L. Perlatum ' in Sect ion Lycope rd on (Demoulin, 1971 ). Hitherto, 3 spe ｭ ci es of Stirps 'L. Perlalum .' are known in the wo rld ( Demoulin. 1971 ). There are 2 spec i es be longing to thi s stir ps known to h ave spongy subgl eba a nd

verr u cose bad isiospores :L. l1 igres ce ns an d L. perlatu l1l

Pers. Pers. (De mo叫 in . 1971) . Ho wever. L. shim ous anll ll1 is

di st i nguished from bot h L. l1 i g 月 sce n s and L. perlatll l1l by the well developed, la rge subgl 巴 ba ， non-p edi ce ll ate basidiospores basidiospores and thick ca pillitial walls, moreover from L. L. Il ig rescells by the pale brownish exope ridi al spin es. 1n 1n the ge nu s Lycoperdoll, ano ther 2 spec i es a r巴 k now n to to h ave spongy, we ll developed. l arge sub gleba lik e L shim ousa/1 um: L. /i vidum .Pers. and L. mamm iJ orme Pe rs. (Calonge, 199 8: Kreise l. 1962 ). Howev巴 r. L. shi ll1 ousa /1l11n. is eas il y distinguished from those 2 spe ci es by its exoper idium forming sub con ica l to conica l spines spines or pulverulent granu les . r etic ul ate endop er idium of gl eba and non-pedicellate bas idio spore s. Only 1 species , L. umbrin oid es Dissing & M .L ang e was previously de sc rib 巴 d to have co nsp icu ｭ ously ously thick- wa ll ed cap illi tium ( 1. 0-2.5 μm broad, Dissing Dissing and Lange, 19 62; Kasuya. 2004c; Sa ras ini . 2005) in in this genus. Howev 巴 r ， this spec i es is clearly sepa ｭ rated rated from L. sh il1l ousanu m by it s blackish. smooth e ndop er idiu 日1 and warty basidiospores w ith long

pedice l. As above, no species of Lyco perd o l1 is known to h ave spongy to a l v 巴 o la te ， we ll developed, l arge subgleba, non.p edi ce ll ate . verrucose basidiospores and thi c k ca pillitial wa ll s. Therefore. L. s him ousa 川 1 111 is deｭ sc rib ed h ere as a new spec ies . Fig. Fig. 8. Lyco perdo ll o5 "i Ill O Il S(/ II/ '''' (C BM.FB .3250. holotype) Observations on the detailed str uctu r es of capillitial A. A. Basid iomata: B. SEM mi crograp h of bas idi ospore: C. wa ll s are diffi cult by the us ual microscopica l methods. .ﾄ uto Au orescence of bas icl iospores and capi lli tia. Sca le bars bars A: 20 mm. B: 1. 0μm. C:1 5μm However, 3- layere d cap illitial wa ll s ca n be clearly ob ｭ serve d und er flu or escence mi croscopy. This result su ggests that the str uct ur es of capillitial wa ll s ca n be globose globose to su bglobo se sphaerocysts. 5.0-7.0μm br oad. possibly applied to a new taxonom ic c haracters. hya lin e Therefore. int ensive re-exa min atio ns on morph o lo幻r H abit at: On ri ch so il or humus in woo dl and of ca pilli tial wa ll s of Ly co perd on usin g fluor esce nce miｭ Di st ribu tion: ]apan (C hib a). Known only from type croscopy are nee d ed loc alit y

Specim en exam in ed: Cl� ba Pre f.. 1mb a -gun. Sakae Lycoperdoll II l1l br;' llIl11 Pers . P 巴 rs. . Synops . Met h machi. machi. Ryukakuji, Octob er 6. 199 1. co l1. Chi ba Myco l. Fung.: 147, 1801 C lub s.n ..CBM-FB - 3250 (holot ype ). Fig.9. ]apa n ese name: Shimousa- as hibuto-hokoritake Basidiomata in sma ll grou ps. 20 -4 0 mm broad. 25 -4 5

quFb A dditions to Lycoperdac eae (Gasteromycetes ) of Chiba. ]apan

Pér巴 z-Silva ， 1989 ). Specimen examined; Chiba Pre f.. Chiba-shi, Chuo-ku, Ao ba-cho. ]une 25. 1998, co ll. Y. Abe s.n.. CBM-FB- 16528; 16528; sa me place, ]uly 1. 1998, co ll. T. Fukiharu s_n CBM -FB -1 6555 and 16556; Chiba Pre f.. Chosei-gun, C h onan-machi. Kasamoriji, S巴 ptember 24, 1994, co ll. E Sano s.n.. CBM-FB -1l 572 ]apanese name; Tsubu-hokoritake.

R 巴 marks; This spec ie s is newly reported here from Chiba Prefecture_ This species is morphologically very

sim il ar to L 凸:Ionicwn Demoulin. but it s basidiospores have long pedicels up to 20μm long ( Demoulin. 1972 ) 1n 1n ]apanes 巴 members of . basidioma strucｭ ture ture of th is species is sim ilar to L. umbrinoides, but the atter latter has more warty basidiospores and the very Jif:!?治 B ~ぶ a thick-wa ll ed cap illi tium ( Dissing and Lang巴 ， 1962; Kasuya, 2004c; Sarasini, 2005 ) 。

Acknowledgements

The se nior author is grateful to Dr. Yosh iji Ando and Dr. Yoshibumi Kikuchi for using the fluorescence microscope and taking the fluorescence photographs Fig. Fig. 9. Lyco perdoll 1I11l brimllll Pers. :Pers. (CBM -FB ・ 11 572 ) We also thank D r. Makoto Kakishima_ reviewing this A .Basidiomata: B. Basidiospores: C. Capillitia. a. capi lli tial thread: thread: b. detail of capillitium. Scale bars A: 7 mm. B: 3.5 manuscrip t. For co ll ect ing specim ens, we are gratefu l μm. Ca: 20μm. Cb: 10μm to M r. 1kuo Asa i and M r. Haruo Sakamoto_ F in all y, we greatly greatly acknowledge D r. Toshimitsu Fuk ih aru (t he mm high . subpyr iform to pyriform, pale brown to yelｭ herbarium of CBM) for loan of the specimens. lowish lowish brown. Exoperidium made of densely. s lender_ References convergent spines , 0.5-1 mm long, pale brown, brown to to blackish brown, l at巴 r sloughin g 0妊. not surround 巴 d A hmad. S. 194 1. Higher fungi of the Panjab Plains. III by granu l es or warts. Endoper idium papery. shiny. The Gasteromycetes . ]. Indian Bo t. Soc. 20: 135-143. pale pale brown to grayish brown at the upper part than A ndo. Y._ M . Sato and K. Fujisaki. 199 1. Ap plication of ulｭ trav io let fluorescence microscop y to the morphological subgleba, lower ochraceous to ye ll owish brown_ Gl 巴 ba c1 ass ifi cation of ticks. ]. Bio l. 91-95 white white when yo ung. l ater oli vaceo us brown to someｭ Photogr二 59: Bottom ley. A. M . 1948. Gasteromycetes of South Africa times times purplish, with indistinct pseudoco lum e ll a. Botha li a 4: 473-810 Subg leba we ll , spongy to alveolate . developed Ca ld er�- V illagom ez . A. and E . Perez -Silva . 1989 oli vaceo us brown to greyish brown. Co nsid e racio 日 es taxonomicas y nuevos registros de Basidiospor巴 s globose to subglobose_ m in ut巴 Iy alguna s es pecies del genero Lycoperdoll ve rrucose. wa rts up to 0.5- 1. 0μm high. (3.5- ) 4.0 -5 .5 (Gasteromyc etes ) en M騙ico . A nal 巴 s Ins t. B iol. Univ μm broad exclud in g ornaments or (4_ 0- ) 4.5-6.5μm Nac . A uL i¥l lex .. S 巴r. Bo t. 59: 1-30 broad including ornaments. olivaceous brown. with a Calonge_ F. D. 1998. Gasteromycetes. 1. Lyco perdal es . shor t. hyaline pedicel up to 1. 0μm long. Bas idi a not N id l1 lariale s. Phallales. Scierodermata les. observed. observed. Capi llitium Lycope rdon-type. 2 . 5-8 . 0 μm T l1 lostomatales. Flo ra Mycologica 1ber i ca 3: 1-271 Ca longe . F. D. and V. Demoulin. 1975. Les broad, elastic, pores abundan t. aseptate. dichotomou sly Gast駻omyc鑼es d'Espagne. Bul l. Soc .Myco l. F r. 91: 247- branch ed_ wa ll s 0.5- 1. 0μm th ick. pitt ed 292. 292. Paracapillitium Paracapillitium usually abundant Coker. Coker. W. C. and N .]. Couc h. 1928. The Gasteromycetes Habita t: On rich so il or humus in wood land_ mainly of easte rn United States and Canada. 201 pp. Univer ｭ under s ity of No rth Ca rolin a Press, Chape l Hill Distribution: Distribution: ]apan. Europe (Ca lon ge and D e moulin. C unningham. G. H. 1944. The Gasterom ycetes of Austraｭ 1975; 1975; Kreise l. 1962; Lange, 1 948; Peg l er el (11... 1995 ) li a and Ne w Zealand. 236 pp. ]. M cI ndoe_ Dunedin East East A frica (Oteino, 1967 ). United States (Co ker and D emoulin. V. 1971. Ly co perdon lI orve gicu lIl Demou lin sp. Couch. Couch. 1928) and Mexico (Ca ld er� -Villag�ez and nov.. a new Gasteromycete with boreo -co ntin en tal

qtυ勺， a Taiga Taiga Kasuya and Ken Katumoto

distribution distribution in Europe and North America. Norw. J. Liu. B. 1984. The Gasteromycetes of China. Beih. Nova Bot 18: 161-167. Hedwigia 76: 1-235. Demoulin. Demoulin. V. 1972. Esp鐵es nouvelles ou m鐵onnues du Martin. M. P. 凶1 997. Exope町ridiωum and spores ぱ0f Ca/ I'.吃叫E genre genre Lycoperdo l1 (Gastéromycら tes). Lejeunia 62: 1-28. t附山111/φ /~円川./判川11削l1 is. Mycotaxon 61: 381-387. Dissing. Dissing. H. and M. Lange. 1962. Gasteromycetes of Congo. Mitche I. D. H. W. Shirley and G. Grimes. 1975. Studies of Bul l. Jard. Bot ﾉta t. Brux. 32: 325 -4 16. Di .l' ciseda (Gasteromycetes) in Colorado. Mycologia 67: D()rfelt. D()rfelt. H. and D. Bumzaa. 1986. Di e Gasteromyceten 586-596. (Bauchpilze) (Bauchpilze) der Mongolischen Volksrepublik. Nova Moravec. Z. 1954. On some species of the genus Discisedll Hedwigia Hedwigia 43: 87-11 1. and other gasteromycetes. Sydowia 8: 27 8- 286. Fan. Fan. L.. B. Liu. and H.-Y. Liu. 1994. The Gasteromycetes Oteino. N. C. 1967. Gasteromycetes of East Africa. III. 。f China: a supplement to Nova Hedwigia Beiheft 76. The genus Lycoperdoll Tournefort ex. Pers. Nytt Mag. Beih. Beih. Nova Hedwigia 108: 1-72 Bo t. 14: 89-95. Grgurinovic. Grgurinovic. C. A. 1997. Larg巴 r fungi of South Australia. Pegler. D. N.. T. Lassoe and B. M. Spooner. 1995. British 725 725 pp. The botanic gardens of Adelaide and State herｭ pu狂balls. earthstars and stinkhorns. 255 pp. Royal barium. barium. Adelaide. Botanic Gardens. Kew. 1mazeki. 1mazeki. R.. Y. Otani and T. Hongo. 1988. Fungi of Japan. Reid. D. 1976. Some Gasteromycetes from Trinidado and 623 623 pp. Yama-kei Pub l.. Tokyo. (I n Japanese) Tobago. Kew Bull. 31: 657-690. 1to. 1to. S. 1959. Mycological flora of J apan. vo l. II. Rick. J. 1930. Lycoperdineas riograndensis. Egatea 15: Basidiomycetes. Basidiomycetes. no. 5. . Gasteromycetales. 19-30. 658 658 pp. Yokendo. Tokyo. (I n Japanese) Ruksawong. P. and T. W. Flege l. 200 1. Thai Mushrooms o. It o. S. and S. 1mai. 1939. Fungi of the Bonin 1slands. II 1. and Other Fungi. 268 pp. National Center for Genetic Trans. Trans. Sapporo Nat Hist Soc. 16: 9- 20. Engineering and Biotechnology BIOTEC. Bangkok. Kasuya. Kasuya. T. 2004a. Gasteromycetes of Chiba Prefecture. Sarasini. M. 2005. Gasteromiceti epigei. 406 pp. A. M.B. central central Honshu. Japan. 1. The family Lycoperdaceae. ]. Fondazione Centro Studi Micologici. Vicenza. Nat Hist Mus. 1r隠し Chiba 8: 1-1 1. Shvarcman. S. R. and N. M. Filimonova. 1970. Kasuya. Kasuya. T. 2004b. Autonomous fluorescence of hyphae of Gasteromicety: Gasteromycetes. Flora Sporovych the the Gasteromycetes (Hymenomycetes. ) Rastenij Kazachstana. Tom 1V. 318 pp. Nauka and its taxonomic significance. 111 Watanabe. M. M.. K Academica SS R. Alma-Ata. (I n Russian with Latin deｭ SuzukiandT.Seki (edsよ 1nnovative Roles of Biological scriptions) Resource Resource Centers. pp.57 4- 575. Japan Society for Culｭ Urista. E.. J. Garcia. and J. Castillo. 1985. AIgunas especies ture ture Collections and World Federation for Culture Col- de Gasteromycetos del Norte de Mexico. Rev. Mex. lections. lections. Tsukuba. Mico l. 1: 471・523. Kasuya. Kasuya. T. 2004c. Notes on Japanese Lycoperdaceae. 1: Yoshimi. S. and T. Hongo. 1989. Gasteromycetes. /11

Lycoperdo l1 I/ mbri l1 oides. a tropical newly found in lmazeki. R. and T. Hongo (edsふ Colored Ill ustrations of apan. Japan. Mycoscience 45: 29 8- 300. Mushrooms of Japan. Vo l. II. pp. 193-228. Hoikusha. Kasuya. Kasuya. T. 2∞5. Notes on Japanese Lycoperdaceae. 3. Osaka. (In Japanese) The genus Calvalia in the herbarium of Hiratsuka City Zeller. S. M. and A. H. Smith. 1964. The genus Cll/vmia in Museum. Na t. Envir. Sci. Res. 18: 41-46. North America Ll oydia 27: 14 8- 186. Kasuya. Kasuya. T. and A. Retnowati. 2005. Four species of the (Accepted 3 March 2006) genus genus Ca/valia (Lycoperdaceae) from 1ndonesia. 111 Sugiyama,]. (edよ Program and Abstracts The Myc oｭ logical logical Society of America and The Mycological Society of of Japan Joint Meeting 2005. pp. 136. The Mycological 千葉県菌類誌 (V) 腹菌類. 2. Society Society of Japan. Nara ホコリタケ科の追加 Kers. Kers. L. E. 1975. The genus Disciseda (Gasteromycetes) in in Sweden. Svensk Bo t. Tidskr. 69: 40 5-4 38. 糟谷大河1)・勝本 謙 2) Kreisel. Kreisel. H. 1962. Die Lycoperdaceae der Deutschen Demokratischen Demokratischen Republik. Feddes Reper t. 64: 89-201. 1) 筑波大学生物資源学類植物寄生 l省学研究室 Kreisel. Kreisel. H. 1976. Gasteromyzeten aus Nepal II. Feddes 干 305・8572 茨城県つくば市天王台 1-1-1 Reper t. 87: 83・ 107. E-mail: [email protected] Kreise I. H. 1989. Studies in the Ca/vatia complex 2) 干 753-0214 山口市大内御堀 2055・27 (Basidiomycetes). (Basidiomycetes). Nova Hedwigia 48: 281-296. Lange. Lange. M. 1948. Macromycetes Part 1. The Gasteroｭ mycetes mycetes of Greenland. Medd. Groenland 147: 1-32. 前報にヲ|き続き，千葉県立中央博物館に収蔵されて Lange. Lange. M. 1990. Arctic Gasteromycetes II. Ca/valia in いる千葉県産ホコリタケ科菌類標本について分類学的 Greenland. Greenland. Svalbard. and Iceland. Nord. J. Bo t. 9: 525- 検討をおこない，以下の 8 種を報告した: Cal悶 tÎa

546. 546. bO Il ÎIl 日 ， SÎS ( オオノウタケ). c. cra l1 ﾎiformﾎs ( ノウタケ).

38 38 - Additions Additions to Lycoperdaceae (Gasteromycetes) of Chiba. ]apan

仁 rllgosa ( イロガ、ワリホコリタケ) .仁川ri!(J rll1 is ( キク boninensis. C. craniゲィ)r111I.\' ， 仁川 Igosα.C目 lI tr!ゲormis ， およ メタケ). Disciseda candida ( ウスイロドングリタケ， び L. umbrinum は千葉県加j'jm 干重である.ノウタケ属，

新称). L. nigresce Jl s (クロホコリタケ). L. Shill1(}lI Sallll 川 ドングリタケ腐およびホコリタケ属に属する 4 積の l符 (シモウサアシブトホコリタケ，新初、) .および L について，蛍光顕微鏡飢祭に基づき，胞子と弾糸に認

1I11J brill 1l1l1 (ツブホコリタケ) .これらのうち. 1!!l tl: )，I~ められた自家ill:光を記載した.これらの自家蛍光の観 部が発達し. 11 包子の小柄を欠き，さらに 3 ";;1 からなる 察は，ホコリタケ科の形態分類において重要である可 厚い弾糸墜を持つ新種 L. shimOllSa Jl lll l1 を記載した.ま 能性が示唆された. た .Dι仰 dida は日本新産種である.さらに. c

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