Development 129, 5789-5801 5789 © 2002 The Company of Biologists Ltd doi:10.1242/dev.00155 Cell lineage analysis of the amphipod crustacean Parhyale hawaiensis reveals an early restriction of cell fates Matthias Gerberding1,3, William E. Browne2 and Nipam H. Patel1,3,* 1Department of Organismal Biology and Anatomy, University of Chicago, Chicago, IL 60637, USA 2Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA 3Howard Hughes Medical Institute, University of Chicago, Chicago, IL 60637, USA *Author for correspondence (e-mail:
[email protected]) Accepted 11 September 2002 SUMMARY In the amphipod crustacean, Parhyale hawaiensis, the endoderm and the fourth micromere generates the first few embryonic cleavages are total and generate a germline. These findings demonstrate for the first time a stereotypical arrangement of cells. In particular, at the total cleavage pattern in an arthropod which results in an eight-cell stage there are four macromeres and four invariant cell fate of the blastomeres, but notably, the cell micromeres, and each of these cells is uniquely identifiable. lineage pattern of Parhyale reported shows no clear We describe our studies of the cell fate pattern of these resemblance to those found in spiralians, nematodes or eight blastomeres, and find that the eight clones resulting deuterostomes. Finally, the techniques we have developed from these cells set up distinct cell lineages that differ in for the analysis of Parhyale development suggest that this terms of proliferation, migration and cell fate. Remarkably, arthropod may be particularly useful for future functional the cell fate of each blastomere is restricted to a single analyses of crustacean development.