DOCSLIB.ORG

Genetic Divergence Between Populations Of

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Genetic Divergence Between Populations Of Genetics and Molecular Biology, 30, 3, 667-675 (2007) Copyright by the Brazilian Society of Genetics. Printed in Brazil www.sbg.org.br Research Article Genetic divergence between populations of the stingless bee uruçu amarela (Melipona rufiventris group, Hymenoptera, Meliponini): Is there a new Melipona species in the Brazilian state of Minas Gerais? Mara Garcia Tavares, Luiz Antonio dos Santos Dias, Andréia Arantes Borges, Denilce Meneses Lopes, Angélica Helene Pereira Busse, Ronaldo Guimarães Costa, Tânia Maria Fernandes Salomão and Lucio Antonio de Oliveira Campos Departamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa, MG, Brazil. Abstract Allozyme, microsatellite and random amplified polymorphic DNA (RAPD) molecular markers were used to investi- gate the within and between population genetic variability and between population genetic differentiation of the Bra- zilian stingless bee uruçu amarela (nominally Melipona rufiventris Lepeletier, 1836) present in savanna and Atlantic forest habitats of the Brazilian state of Minas Gerais (MG). We found low levels of within population variability, al- though there were a large number of private alleles that specifically characterized these populations. The FST values indicated a high level of genetic diversity between populations. Analysis of molecular variance (AMOVA) showed a high degree of population differentiation between the savanna and Atlantic forest habitats, confirmed by population pairwise FST data. Principal coordinates analysis and unweighted pair-group method using an arithmetic average (UPGMA) dendrograms also confirmed that in Minas Gerais the savanna populations (M. rufiventris) were geneti- cally distinct from those present in the Atlantic forest (M. mondury). In addition, populations from locations near the towns of Dom Bosco and Brasilândia de Minas were genetically different from those collected in other localities in the savanna. Our data indicate that populations of uruçu amarela found in the savanna and Atlantic forest habitats of Minas Gerais state should be treated separately for conservation purposes and that special attention should be given to the populations found in the region of Dom Bosco and Brasilândia de Minas until their taxonomic status is clarified. Key words: endangered species, genetic divergence, molecular markers, stingless bees, uruçu amarela. Received: May 26, 2006; Accepted: January 31, 2007. Introduction sociated habitat types and it is possible that many locally In Brazil there are approximately 192 species of adapted ecotypes have arisen. For instance, variation in re- stingless bees (Silveira et al., 2002), which play an impor- lation to the color of the posterior legs of M. rufiventris was tant role in the pollination of a large number of wild plants found when different populations were analyzed by Moure and crops in Brazilian ecosystems (Kerr et al., 1996). How- and Kerr (1950) and Moure (1975), who reported that there ever, Campos (1998) stated that populations of the stingless were some specimens almost without black spots on the bee Melipona rufiventris (uruçu amarela) were being dras- posterior tibia in the states of Minas Gerais, Rio de Janeiro, tically reduced due to deforestation, predatory honey col- São Paulo and Paraná while specimens from Bahia, Goiás, lecting and widespread burning, leading to the inclusion of São Paulo and Santa Catarina presented a large black spot these bees in the endangered species list of the Brazilian on the apical portion of the posterior tibia and in all basi- state of Minas Gerais. tarsus. The corbicula and tergal hairs also presented vari- Populations of uruçu amarela occur in southeastern ability in the specimens analyzed, which led to the sugges- Brazil from the state of Bahia to the state of Santa Catarina tion that there was much to be done for a better knowledge and have usually been considered as a single species, M. of the different forms attributed to this species (Moure, rufiventris (Moure, 1975) but such an extensive distribu- 1992). tion encompasses diverse environmental conditions and as- In 2002, our laboratory initiated a project to analyze the genetic variation among populations of uruçu amarela collected in the savanna and in the Atlantic forest of Minas Send correspondence to Mara Garcia Tavares. Departamento de Biologia Geral, Universidade Federal de Viçosa, 36570-000 Viço- Gerais state. Biochemical analysis demonstrated different sa, MG, Brazil. E-mail: [email protected]. migration rates of some enzymatic loci (Est-1, Est-2, Est-3, 668 Tavares et al. Est-4, Pep-1, and Mdh-1) between colonies collected in dif- and in Atlantic forest habitats of Minas Gerais state and ferent regions (Costa et al., 2005). also to analyze the genetic differentiation between the pop- Melo (2003) reported morphological evidence to sug- ulations. gest that uruçu amarela populations from southeastern Bra- zil in fact comprised two different species, M. rufiventris Materials and Methods and M. mondury. According to this author, the typical sa- vanna species M. rufiventris occurs in Mato Grosso, north- Genetic material western São Paulo, western Minas Gerais and southern Samples were collected in ten savanna habitats and Goiás, while M. mondury is distributed across the Atlantic seven Atlantic forest habitats localities within Minas Ge- forest where it is found in the states of Bahia, Espírito rais, the geographic distribution and the number of colonies Santo, Rio de Janeiro, Minas Gerais, São Paulo, Paraná and sampled in each locality being show in Figure 1 and Ta- Santa Catarina. As argued by Melo (2003), however, the ble 1. For all statistical analyses, colonies were grouped ac- recognition of these species based on the morphology of cording to locality and each locality was considered as worker bees is very difficult. representing a population. Bees were collected at the en- Studies concerning the genetic variability of these trance of their colonies and transferred, alive, to our Labo- different forms of uruçu amarela may provide relevant data ratory where they were stored at -70 °C until need for on their population structure, kin relationships and gene electrophoretic analyses. Voucher specimens from both flow between remnant populations. These data are very im- biomes were deposited in the insect collection at the Ento- portant for elaborating strategies for their conservation. It is mology Museum of the Universidade Federal de Viçosa also important to bear in mind that less than 9% of the origi- (UFVB) (Viçosa, Minas Gerais, Brazil) and in the bee col- nal Atlantic forest remains and that a great number of tropi- lection of the Universidade Federal de Minas Gerais cal tree species are pollinated by Melipona bees. (UFMG), Belo Horizonte, Minas Gerais, Brazil. Genetic analysis of populations has been greatly en- hanced with the progress of molecular techniques. Con- Allozyme analysis sidering that various classes of molecular markers are now We analyzed 87 colonies with allozyme markers us- available, it is possible to compare the patterns of structure ing eight bees per colony. Individual worker bees were di- revealed by them in order to verify how gene flow, drift and vided into two parts, head/mesosoma and metasoma, which selection govern the distribution of genetic variation within were homogenized in 100 μL of 0.2% (v/v) b-mercapto- and between populations of a given species. ethanol solution and kept on ice. For allozyme analysis the This paper describes the use of allozyme, micro- homogenates were absorbed with Whatman N° 3 filter pa- satellite and random amplified polymorphic DNA (RAPD) pers and applied to standard horizontal 14% (w/v) starch markers to investigate the genetic variability within and be- gels and electrophoresis conducted according to the proce- tween uruçu amarela populations present in the savanna dures of Harris and Hopkinsons (1978). The following en- Figure 1 - Map of Brazil (A) and Minas Gerais (B) state (B) showing the geographic location of the 17 natural populations of uruçu amarela bees ana- lyzed. Genetic divergence between uruçu amarela populations 669 Table 1 - Location and number of uruçu amarela colonies sampled in the acrylamide gel and visualized by staining with 0.2% (w/v) savanna and the Atlantic forest of the Brazilian state of Minas Gerais. Data silver nitrate. The lengths of amplified alleles were checked ordered by increasing latitude. using a 10 bp DNA ladder (Invitrogen, São Paulo, Brazil). Habitat and location (code) Latitude Number of and longitude colonies RAPD analysis Savanna habitat For the RAPD marker analysis we used one bee from Dom Bosco (DB) 16°39’ S, 46°16’ W 2 each of 70 colonies. The PCR-RAPD analyses were per- formed as described by Williams et al. (1990), with some Brasilândia de Minas (BR) 17°00’ S, 46°00’ W 5 modifications. Each PCR reaction used a total volume of Patos de Minas (PA) 18°34’ S, 46°31’ W 6 25 μL with 1x reaction buffer, 0.1 mM dNTPs, 0.4 μM Guimarânia (GU) 18°50’ S, 46°47’ W 20 10-base primer (Operon Technologies, Alameda, CA, Patrocínio (PT) 18°56’ S, 46°59’ W 2 USA), 0.5 U Taq polymerase (Phoneutria, Belo Horizonte, Pequi (PE) 19°37’ S, 44°39’ W 2 Minas Gerais, Brazil) and 2 μL template DNA (25 ng). Am- Uberaba (UB) 19°44’ S, 47°55’ W 2 plification was in a PTC-100 thermocycler (MJ Research) Córrego Danta (CD) 19°49’ S, 45°54’ W 4 programmed for 40 cycles, each cycle consisting of 15 s at Arcos (AR) 20°16’ S, 45°32’ W 4 94 °C, 30 s at 35 °C and 1 min at 72 °C, with a final exten- th Formiga (FO) 20°27’ S, 45°25’ W 2 sion after the 40 cycle for 7 min at 72 °C. The amplifica- tion products were separated by electrophoresis in 1.2% Subtotal for savanna colonies 49 (w/v) agarose gels in TBE (90 mM Tris-borate pH 8.0, Atlantic forest habitat 10 mM EDTA) buffer and stained with ethidium bromide Poté (PO) 17°48’ S, 41°47’ W 5 (0.2 μg/mL).
Load more

Recommended publications
  • Bumblebees' Bombus Impatiens
    Bumblebees’ Bombus impatiens (Cresson) Learning: An Ecological Context by Hamida B. Mirwan A Thesis Presented to The University of Guelph In partial fulfillment of requirements for the degree of Doctor of Philosophy in School of Environmental Biology Guelph, Ontario, Canada © Hamida B. Mirwan, August, 2014 ABSTRACT BUMBLEBEES’ BOMBUS IMPATIENS (CRESSON) LEARNING: AN ECOLOGICAL CONTEXT Hamida B. Mirwan Co-Advisors: University of Guelph, 2014 Professors Peter G. Kevan & Jonathan Newman The capacities of the bumblebee, Bombus impatiens (Cresson), for learning and cognition were investigated by conditioning with increasingly complex series of single or multiple tasks to obtain the reinforcer (50% sucrose solution). Through operant conditioning, bumblebees could displace variously sized combinations of caps, rotate discs through various arcs (to 180°), and associate rotation direction with colour (white vs. yellow). They overcame various tasks through experience, presumably by shaping and scaffold learning. They showed incremental learning, if they had progressed through a series of easier tasks, single caps with increasing displacement complexity (to left, right, or up) or of balls with increasing masses, but could not complete the most difficult task de novo. They learned to discriminate the number of objects in artificial flower patches with one to three nectary flowers presented simultaneously in three compartments, and include chain responses with three other tasks: sliding doors, lifting caps, and rotating discs presented in fixed order. Pattern recognition and counting are parts of the foraging strategies of bumblebees. Multiple turn mazes, with several dead ends and minimal visual cues, were used to test the abilities of bumblebees to navigate by walking and remember routes after several days.
    [Show full text]
  • Classification of the Apidae (Hymenoptera)
    Utah State University DigitalCommons@USU Mi Bee Lab 9-21-1990 Classification of the Apidae (Hymenoptera) Charles D. Michener University of Kansas Follow this and additional works at: https://digitalcommons.usu.edu/bee_lab_mi Part of the Entomology Commons Recommended Citation Michener, Charles D., "Classification of the Apidae (Hymenoptera)" (1990). Mi. Paper 153. https://digitalcommons.usu.edu/bee_lab_mi/153 This Article is brought to you for free and open access by the Bee Lab at DigitalCommons@USU. It has been accepted for inclusion in Mi by an authorized administrator of DigitalCommons@USU. For more information, please contact [email protected]. 4 WWvyvlrWryrXvW-WvWrW^^ I • • •_ ••^«_«).•>.• •.*.« THE UNIVERSITY OF KANSAS SCIENC5;^ULLETIN LIBRARY Vol. 54, No. 4, pp. 75-164 Sept. 21,1990 OCT 23 1990 HARVARD Classification of the Apidae^ (Hymenoptera) BY Charles D. Michener'^ Appendix: Trigona genalis Friese, a Hitherto Unplaced New Guinea Species BY Charles D. Michener and Shoichi F. Sakagami'^ CONTENTS Abstract 76 Introduction 76 Terminology and Materials 77 Analysis of Relationships among Apid Subfamilies 79 Key to the Subfamilies of Apidae 84 Subfamily Meliponinae 84 Description, 84; Larva, 85; Nest, 85; Social Behavior, 85; Distribution, 85 Relationships among Meliponine Genera 85 History, 85; Analysis, 86; Biogeography, 96; Behavior, 97; Labial palpi, 99; Wing venation, 99; Male genitalia, 102; Poison glands, 103; Chromosome numbers, 103; Convergence, 104; Classificatory questions, 104 Fossil Meliponinae 105 Meliponorytes,
    [Show full text]
  • Tetragonula Carbonaria and Disease: Behavioural and Antimicrobial Defences Used by Colonies to Limit Brood Pathogens
    Tetragonula carbonaria and disease: Behavioural and antimicrobial defences used by colonies to limit brood pathogens Jenny Lee Shanks BHort, BSc (Hons) Submitted in fulfilment of requirements for the degree Doctor of Philosophy Submitted to the School of Science and Health University of Western Sydney, Hawkesbury Campus July, 2015 Our treasure lies in the beehive of our knowledge. We are perpetually on the way thither, being by nature winged insects and honey gatherers of the mind. Friedrich Nietzsche (1844 – 1900) i Statement of Authentication The work presented in this thesis is, to the best of my knowledge and belief, original except as acknowledged in the text. I hereby declare that I have not submitted this material, whether in full or in part, for a degree at this or any other institution ……………………………………………………………………. Jenny Shanks July 2015 ii Acknowledgements First and foremost, I am extremely indebted to my supervisors, Associate Professor Robert Spooner-Hart, Dr Tony Haigh and Associate Professor Markus Riegler. Their guidance, support and encouragement throughout this entire journey, has provided me with many wonderful and unique opportunities to learn and develop as a person and a researcher. I thank you all for having an open door, lending an ear, and having a stack of tissues handy. I am truly grateful and appreciate Roberts’s time and commitment into my thesis and me. I am privileged I had the opportunity to work alongside someone with a wealth of knowledge and experience. Robert’s passion and enthusiasm has created some lasting memories, and certainly has encouraged me to continue pursuing my own desires.
    [Show full text]
  • Pollen Harvest by Africanized Apis Mellifera and Trigona Spinipes in São Paulo Botanical and Ecological Views M
    POLLEN HARVEST BY AFRICANIZED APIS MELLIFERA AND TRIGONA SPINIPES IN SÃO PAULO BOTANICAL AND ECOLOGICAL VIEWS M. Cortopassi-Laurino, M. Ramalho To cite this version: M. Cortopassi-Laurino, M. Ramalho. POLLEN HARVEST BY AFRICANIZED APIS MELLIFERA AND TRIGONA SPINIPES IN SÃO PAULO BOTANICAL AND ECOLOGICAL VIEWS. Apidolo- gie, Springer Verlag, 1988, 19 (1), pp.1-24. hal-00890725 HAL Id: hal-00890725 https://hal.archives-ouvertes.fr/hal-00890725 Submitted on 1 Jan 1988 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. POLLEN HARVEST BY AFRICANIZED APIS MELLIFERA AND TRIGONA SPINIPES IN SÃO PAULO BOTANICAL AND ECOLOGICAL VIEWS M. CORTOPASSI-LAURINO M. RAMALHO Departamento de Ecología Cera1 do lnstituto de Biociências da Universidade de São Paulo, 05508 São Paulo, Brasíl SUMMARY During one year, monthly samples of pollen were taken from one colony of Apis mellifera and one colony of Trigona spinipes. A great number of pollen types was observed in each of the samples (approximately 40), although few sources were intensively visited each month. T. spinipes collected significantly from Eucalyptus spp., Aloe sp. and Archontophoenix sp., and A. mellifera visited mainly Eucalyptus spp., Tipuana speciosa, Caesalpinia peltophoroides, Mikania glomerata and Cecropia sp.
    [Show full text]
  • Warfare in Stingless Bees
    Insect. Soc. (2016) 63:223–236 DOI 10.1007/s00040-016-0468-0 Insectes Sociaux REVIEW ARTICLE Warfare in stingless bees 1,2 1,3 4 5 C. Gru¨ter • L. G. von Zuben • F. H. I. D. Segers • J. P. Cunningham Received: 24 August 2015 / Revised: 28 January 2016 / Accepted: 6 February 2016 / Published online: 29 February 2016 Ó International Union for the Study of Social Insects (IUSSI) 2016 Abstract Bees are well known for being industrious pol- how victim colonies are selected, but a phylogenetically linators. Some species, however, have taken to invading the controlled analysis suggests that the notorious robber bee nests of other colonies to steal food, nest material or the nest Lestrimelitta preferentially attacks colonies of species with site itself. Despite the potential mortality costs due to more concentrated honey. Warfare among bees poses many fighting with an aggressive opponent, the prospects of a interesting questions, including why species differ so large bounty can be worth the risk. In this review, we aim to greatly in their response to attacks and how these alternative bring together current knowledge on intercolony fighting strategies of obtaining food or new nest sites have evolved. with a view to better understand the evolution of warfare in bees and identify avenues for future research. A review of Keywords Stingless bees Á Warfare Á literature reveals that at least 60 species of stingless bees are Alternative foraging strategies Á Cleptoparasitism Á involved in heterospecific conflicts, either as attacking or Lestrimelitta Á Meliponini victim colonies. The threat of invasion has led to the evo- lution of architectural, behavioural and morphological adaptations, such as narrow entrance tunnels, mud balls to Introduction block the entrance, decoy nests that direct invaders away from the brood chamber, fighting swarms, and soldiers that The nest is the all-important centre of the bee’s universe, are skilled at immobilising attackers.
    [Show full text]
  • Redalyc.Honey Physicochemical Properties of Three Species of The
    Anais da Academia Brasileira de Ciências ISSN: 0001-3765 [email protected] Academia Brasileira de Ciências Brasil Lage, Lorena G.A.; Coelho, Lívia L.; Resende, Helder C.; Tavares, Mara G.; Campos, Lucio A.O.; Fernandes-Salomão, Tânia M. Honey physicochemical properties of three species of the brazilian Melipona Anais da Academia Brasileira de Ciências, vol. 84, núm. 3, septiembre, 2012, pp. 605-608 Academia Brasileira de Ciências Rio de Janeiro, Brasil Available in: http://www.redalyc.org/articulo.oa?id=32723112027 How to cite Complete issue Scientific Information System More information about this article Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Journal's homepage in redalyc.org Non-profit academic project, developed under the open access initiative Anais da Academia Brasileira de Ciências (2012) 84(3): 605-608 (Annals of the Brazilian Academy of Sciences) Printed version ISSN 0001-3765 / Online version ISSN 1678-2690 www.scielo.br/aabc Honey physicochemical properties of three species of the brazilian Melipona LORENA G.A. LAGE, LÍVIA L. COELHO, HELDER C. RESENDE, MARA G. TAVARES, LUCIO A.O. CAMPOS and TÂNIA M. FERNANDES-SALOMÃO Departamento de Biologia Geral, Universidade Federal de Viçosa, Avenida PH Rolfs, s/n, 36570-000 Viçosa, MG, Brasil Manuscript received on April 19, 2010; accepted for publication on December 16, 2011 ABSTRACT Physicochemical analyses were carried out to evaluate 27 samples of honeys from three species of the Brazilian genus Melipona (M. capixaba, M. rufiventris and M. mondury) from Espírito Santo and Minas Gerais States. The parameters water activity (Aw), percentage of soluble solids (Brix %), pH, acidity (meq/Kg) and moisture (%) were evaluated.
    [Show full text]
  • Orsis 30: 91-40
    Universitat Autònoma de Barcelona Departament de Biologia Animal, de Biologia Vegetal i d’Ecologia ORSIS Organismes i Sistemes 31 Vol. 31, 2017 ISSN 0213-4039 (imprès), 2014-9727 (en línia), http://revistes.uab.cat/orsis Servei de Publicacions DADES CATALOGRÀFIQUES RECOMANADES PEL SERVEI DE BIBLIOTEQUES DE LA UNIVERSITAT AUTÒNOMA DE BARCELONA Orsis Orsis : organismes i sistemes : revista de botànica, zoologia i ecologia / Departaments de Botànica, d’Ecologia i de Zoologia de la Universitat Autònoma de Barcelona. — Núm. 1 (1985)-. — Bellaterra : Servei de Publicacions de la Universitat Autònoma de Barcelona, 1985-. — 23 cm Anual. — A partir del núm. 3 (1988) la menció de responsabilitat correspon a les unitats de Botànica, Zoologia i Ecologia del Departament de Biologia Animal, Biologia Vegetal i Ecologia de la Universitat Autònoma de Barcelona. ISSN 0213-4039 I. Universitat Autònoma de Barcelona. Departament de Botànica, Departament d’Ecologia, Depar- tament de Zoologia, Laboratori de Botànica, Laboratori de Zoologia, Laboratori d’Ecologia. 1. Botànica, zoologia i ecologia — Revistes 2. Ciències naturals — Revistes 58, 59, 574 Editor Subscripció i administració Llorenç Sáez Gonyalons Universitat Autònoma de Barcelona (Universitat Autònoma de Barcelona) Servei de Publicacions 08193 Bellaterra (Barcelona). Spain Editors associats Tel. 93581 10 22. Fax 93 581 32 39 Xavier Espadaler (Universitat Autònoma [email protected] de Barcelona) www.uab.cat/publicacions Jacint Ventura (Universitat Autònoma de Barcelona) Intercanvi Francesc Muñoz (Universitat
    [Show full text]
  • Floral Preferences and Climate Influence in Nectar and Pollen
    November - December 2010 879 ECOLOGY, BEHAVIOR AND BIONOMICS Floral Preferences and Climate Infl uence in Nectar and Pollen Foraging by Melipona rufi ventris Lepeletier (Hymenoptera: Meliponini) in Ubatuba, São Paulo State, Brazil ADRIANA DE O FIDALGO1, ASTRID DE M P KLEINERT2 1Seção de Sementes e Melhoramento Vegetal, Instituto de Botânica, Av Miguel Estéfano, 3687, 04301-012 São Paulo, SP, Brasil; aofi [email protected] 2Lab de Abelhas, Depto de Ecologia, Instituto de Biociências, Univ de São Paulo, Rua do Matão, tr. 14, 321, 05508-900 São Paulo, SP, Brasil; [email protected] Edited by Kleber Del Claro – UFU Neotropical Entomology 39(6):879-884 (2010) ABSTRACT - We describe the environment effects on the amount and quality of resources collected by Melipona rufi ventris Lepeletier in the Atlantic Forest at Ubatuba city, São Paulo state, Brazil (44o 48’W, 23o 22’S). Bees carrying pollen and/or nectar were captured at nest entrances during 5 min every hour, from sunrise to sunset, once a month. Pollen loads were counted and saved for acetolysis. Nectar was collected, the volume was determined and the total dissolved solids were determined by refractometer. Air temperature, relative humidity and light intensity were also registered. The number of pollen loads reached its maximum value between 70% and 90% of relative humidity and 18 oC and 23oC; for nectar loads this range was broader, 50-90% and 20-30ºC. The number of pollen loads increased as relative humidity rose (rs = 0.401; P < 0.01) and high temperatures had a strong negative infl uence on the number of pollen loads collected (rs = -0.228; P < 0.01).
    [Show full text]
  • The First Report of Melipona (Michmelia) Rufiventris Lepeletier, 1836 in the Municipality of Sacramento, Minas Gerais, Brazil
    E T O N C I F I T N E I C S THE FIRST REPORT OF MELIPONA (MICHMELIA) RUFIVENTRIS LEPELETIER, 1836 IN THE MUNICIPALITY OF SACRAMENTO, MINAS GERAIS, BRAZIL THIAGO HENRIQUE AZEVEDO TOSTA FERNANDA HELENA NOGUEIRA­FERREIRA Universidade Federal de Uberlândia, Instituto de Biologia, Programa de Pós­graduação em Ecologia e Conservação de Recursos Naturais, Rua Ceará, s/n, Bairro Umuarama, 38405­302, Uberlândia, Minas Gerais, Brazil, [email protected] Abstract: A survey of the apifauna was performed in a conservation area formed by phytophysiognomies of a Cerrado Biome located in Sacramento, Minas Gerais. The sampling of bees was carried out through collecting individuals during their flower visits, using aromatic baits, and actively searching for nests. Seventeen bee species were registered, among them, a nest of Melipona rufiventris. Considering that this species had never been registered in the municipality of Sacramento (Minas Gerais, Brazil) and that it is also a species endemic to the Cerrado and in danger of extinction, dependent on protected remnants, and that suffers from several anthropic actions, this note has relevance and contributes to the biogeographic knowledge of the species. 26 Keywords: endemic species, Savannah, stingless bee. O PRIMEIRO REGISTRO DE MELIPONA (MICHMELIA) RUFIVENTRIS LEPELETIER, 1836 NO MUNICÍPIO DE SACRAMENTO, MINAS GERAIS, BRASIL Resumo: Foi realizado um levantamento da apifauna em uma unidade de conservação formada por fitofisionomias do bioma Cerrado localizada no município de Sacramento, Minas Gerais. A amostragem das abelhas foi realizada por meio da coleta de indivíduos durante as visitas às flores, com uso de iscas aromáticas e busca ativa de ninhos.
    [Show full text]
  • Thermogenesis in Stingless Bees: an Approach with Emphasis on Brood's Thermal Contribution
    J Anim Behav Biometeorol ISSN 2318-1265 v.4, n.4, p.101-108 (2016) REVIEW Thermogenesis in stingless bees: an approach with emphasis on brood's thermal contribution Maiko Roberto Tavares Dantas MRT Dantas (Corresponding author) email: [email protected] Department of Animal Sciences, Universidade Federal Rural do Semi-Árido (UFERSA), Mossoró, RN, Brazil. Received: August 09, 2016 ▪ Revised: September 08, 2016 ▪ Accepted: September 12, 2016 Abstract The animals behave as a thermodynamic system development, productivity and reproduction in its various complex, which remains all the time exchanging energy with segments (Heinrich 1981; Heinrich 1993; Heinrich 1994; the environment. In this context, the body temperature of Mardan and Kevan 2002; Roldão 2011). The body bees considerably accompanies variations in ambient temperature of an animal refers to the quantity of stored temperature, and the performance of most of its activity is thermal energy per unit of body mass. This energy can be largely affected by air temperature. When these individuals increased or decreased by thermolysis and thermogenesis are exposed to temperatures above or below the optimum processes, respectively (Silva 2000). In these processes are range for the species during its pupal stage, these, when they involved behavioral, autonomous and adaptive mechanisms survive, have morphological deficiencies, physiological or (Silva 2000). behavioral as adults. These insects use physiological The animals act as a thermodynamic complex system, activities such as internal temperature control mechanisms of which remains all the time exchanging energy with the the nest. Social insects like honey bees demonstrate certain environment (Silva 2000). Due to this process, the ambient thermoregulatory ability to nest in which they live, known as tends to induce physiological changes in such organisms in the colonial endotherm.
    [Show full text]
  • ECOLOGIA QUÍMICA DE ABELHAS BRASILEIRAS: Melipona Rufiventris
    I UNIVERSIDADE ESTADUAL DE CAMPINAS INSTITUTO DE QUÍMICA ECOLOGIA QUÍMICA DE ABELHAS BRASILEIRAS: Melipona rufiventris, Melipona scutellaris, Plebeia droryana, Nannotrigona testaceicornis, Tetragonisca angustula e Centris trigonoides. ADRIANA PIANARO Dissertação de Mestrado Orientadora: Profa. Dra. Anita Jocelyne Marsaioli Campinas - São Paulo Fevereiro/2007 II V DEDICATÓRIA: Aos meus pais, Alceu e Malta; As minhas irmãs, Valéria e Priscila; Ao meu namorado, Armando Mateus; E ao casal Armando e Shirley e sua filha Juliana. VII AGRADECIMENTOS Primeiramente, eu gostaria de agradecer a Deus, por ter dado forças e iluminado meu caminho durante todos estes anos de vida. Aos meus queridos pais Alceu e Malta, pelo carinho, amor e compreensão dedicados a mim. As minhas irmãs Valéria e Priscila, pelo amor e pela amizade. Ao meu namorado Armando Mateus, por ser esta pessoa maravilhosa e companheira. Aos seus pais Shirley e Armando e sua irmã Juliana, pelo carinho e pela amizade. A Profa. Dra. Anita Jocelyne Marsaioli, minha orientadora, pela amizade, confiança e orientação durante o desenvolvimento deste trabalho. Aos meus colaboradores, Prof. Dr. Paulo Nogueira Neto, Profa. Dra. Eda Flávia L. R. A. Patricio, Profa. Dra. Adriana Flach, Profa. Dra. Mariza G. Reis, Prof. Dr. Rodrigo B. Singer, Prof. Dr. Gabriel A. R. Melo, Prof. Dr. Warwick E. Kerr e ao aluno Cristiano Menezes, pela amizade e pela importante contribuição oferecida para este trabalho resultar em uma dissertação de mestrado. Aos meus colegas de grupo de pesquisa: Simone, Georgiana, Cabeça, Isis, Luciana, Lu Chen, Lucimar, Eduardo, Carla, Marcela, Diego e Suzan, pela amizade e o companheirismo durante estes anos. Agradeço aos meus colegas que já são professores em universidades: Dr.
    [Show full text]
  • Aspectos Da Biologia De Melipona Rufiventris Lepeletier, 1836 E De Melipona Mondury Smith, 1863 (Hymenoptera: Apidae, Meliponina)
    MARIA AUGUSTA PEREIRA LIMA Aspectos da biologia de Melipona rufiventris Lepeletier, 1836 e de Melipona mondury Smith, 1863 (Hymenoptera: Apidae, Meliponina) Tese apresentada à Universidade Federal de Viçosa como parte das exigências do Programa de Pós-Graduação em Entomologia, para obtenção do título de “Magister Scientiae”. VIÇOSA MINAS GERAIS – BRASIL 2004 Ficha catalográfica preparada pela Seção de Catalogação e Classificação da Biblioteca Central da UFV T Lima, Maria Augusta Pereira, 1976- L732a Aspectos da biologia de Melipona rufiventris 2004 Lepeletier, 1836 e de Melipona mondury Smith, 1863 (Hymenoptera: Apidae, Meliponina / Maria Augusta Pereira Lima. – Viçosa : UFV, 2004. vii, 65f. : il. ; 29cm. Orientador: Lucio Antonio de Oliveira Campos. Dissertação (mestrado) - Universidade Federal de Viçosa. Inclui bibliografia. 1. Melipona rufiventris - Biologia. 2. Melipona mondury - Biologia. 3. Melipona mondury - Reprodução. I. Universidade Federal de Viçosa. II.Título. CDD 20.ed. 595.79045 MARIA AUGUSTA PEREIRA LIMA Aspectos da biologia de Melipona rufiventris Lepeletier, 1836 e de Melipona mondury Smith, 1863 (Hymenoptera: Apidae, Meliponina) Tese apresentada à Universidade Federal de Viçosa como parte das exigências do Programa de Pós-Graduação em Entomologia, para obtenção do título de “Magister Scientiae”. Aprovada: 12 de fevereiro de 2004 ________________________ __________________________ Prof. José Lino Neto Profa. Mara Garcia Tavares (Conselheiro) (Conselheira) ________________________ __________________________ Dr. Rui Carlos Peruquetti Profa. Georgina Maria de Faria Mucci ________________________ Prof. Lucio Antonio de Oliveira Campos (Orientador) Agradecimentos Agradecer através de breves palavras é apenas um pequeno gesto da minha mais sincera gratidão àqueles que colaboraram direta, ou indiretamente, para a realização deste trabalho. Primeiramente, gostaria de agradecer à minha família: mamãe e papai, os maiores responsáveis por eu ter chegado até aqui; Ana Paula e Ugo, pelo amor, incentivo e companheirismo.
    [Show full text]