DOCSLIB.ORG

Galectin-1, the New Player in Staging and Prognosis of COVID-19

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Galectin-1, the New Player in Staging and Prognosis of COVID-19 Galectin-1, The New Player in Staging and Prognosis of COVID-19 Soja Sekulic University of Kragujevac, Faculty of Medical Sciences, Department of Infectious disease Nevena Gajovic University of Kragujevac, Faculty of Medical Sciences, Center for Molecular Medicine and Stem Cell Research, Milena Jurisevic University of Kragujevac, Faculty of Medical Sciences, Department of Clinical pharmacy Marina Jovanovic ( [email protected] ) University of Kragujevac, Faculty of Medical Sciences, Department of Internal medicine Biljana Popovska Jovicic University of Kragujevac, Faculty of Medical Sciences, Department of Infectious disease Nebojsa Arsenijevic University of Kragujevac, Faculty of Medical Sciences, Center for Molecular Medicine and Stem Cell Research, Zeljko Mijailovic University of Kragujevac, Faculty of Medical Sciences, Department of Infectious disease Marina Jovanovic University of Kragujevac, Faculty of Medical Sciences, Department of Otorinolaringology Zana Dolicanin State University of Novi Pazar, Department of Biomedical sciences Ivan Jovanovic University of Kragujevac, Faculty of Medical Sciences, Center for Molecular Medicine and Stem Cell Research, Research Article Keywords: galectin-1, COVID-19, prognostic marker Posted Date: September 3rd, 2021 DOI: https://doi.org/10.21203/rs.3.rs-830345/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Galectin-1, the new player in staging and prognosis of COVID-19 Running title: Galectin-1 in COVID-19 progression Sofija Sekulic1, Nevena Gajovic2, Milena Jurisevic3, Marina Jovanovic*4, Biljana Popovska Jovicic1, Nebojsa Arsenijevic2, 5, Zeljko Mijailovic1, Marina Jovanovic6, Zana Dolicanin7, Ivan Jovanovic2 1 University of Kragujevac, Faculty of Medical Sciences, Department of Infectious disease, Kragujevac, 34000, Serbia 2 University of Kragujevac, Faculty of Medical Sciences, Center for Molecular Medicine and Stem Cell Research, Kragujevac, 34000, Serbia 3 University of Kragujevac, Faculty of Medical Sciences, Department of Clinical pharmacy, Kragujevac, 34000, Serbia 4 University of Kragujevac, Faculty of Medical Sciences, Department of Internal medicine, Kragujevac, 34000, Serbia 5 Public health institute Kragujevac, Kragujevac, 34000, Serbia 6 University of Kragujevac, Faculty of Medical Sciences, Department of Otorinolaringology, Kragujevac, 34000, Serbia 7 State University of Novi Pazar, Department of Biomedical sciences, Novi Pazar, 36300, Serbia Correspondence: Marina Jovanovic*, MD PhD University of Kragujevac, Faculty of Medical Sciences, Department of Internal medicine, Kragujevac, 34000, Serbia Svetozara Markovica 69, 34000 Kragujevac, Serbia Tel +38134306800, Fax. +38134306800112 E-mail: marinna034@gmail. com Key words: galectin-1, COVID-19, prognostic marker Abstract Background A new virus from the group of coronaviruses was identified as the cause of atypical pneumonia and called severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and disease called Corona Virus Disease (COVID-19). During the cytokine storm, the main cause of the death, proinflammatory cytokines are released which stimulate further tissue destruction. Galectin-1 is pleiotropic cytokine involved in many immune and inflammatory processes and its role in COVID-19 is still unknown. Objective The aim of this study was to determine systemic values of galectin-1 and correlations between gal-1 and proinflammatory cytokines and clinical parameters during COVID-19 progression. Design Observational and cross-sectional study. Patients 210 COVID-19 patients were included and divided into mild, severe or critical group according to COVID-19 severity. Main Measures Serum levels of IL-1β, IL-6, IL-10, IL-23, IL- 33 and galectin-1 were measured using sensitive enzyme-linked immunosorbent assay (ELISA) kits. Key Results Systemic levels of IL-1β, IL-6, IL-10, IL-23, IL-33 and galectin-1 were significantly higher in stage III of COVID-19 patients compared to stage I and II. There were no significant differences in ratio between Galectin-1 and IL-10 with proinflammatory cytokines. Positive correlation was detected between Gal-1 and IL-1β, IL6, IL-10, IL-23 and IL-33. Gal-1 positively correlated with chest radiographic finding, dry cough and headache and negatively correlated with normal breathing sound. Conclusions Linear regression model and ROC curve analysis point on Gal-1 as significant predictors for COVID-19 severity. Presented results implicate on Gal-1 and IL-10 dependent immunomodulation. The precise mechanism of Gal-1 effect in COVID-19 and its potential as a stage marker of disease severity is still to be clarified. INTRODUCTION COVID-19 is an infectious respiratory disease caused by the novel coronavirus, named as severe acute respiratory syndrome–corona virus 2 (SARS-CoV2). (1). Briefly after COVID-19 outbreak in China, the disease spread around the world and the World Health Organization declared a pandemic1. The clinical presentation of COVID-19 can vary from asymptomatic to acute respiratory distress syndrome (ARDS) and respiratory insufficiency2, 3. Most common underlying mechanism of respiratory failure in patients with COVID-19 is characterized by rapid activation of proinflammatory cytokines and inflammatory cell recruitment in the lungs (e. g. cytokine storm)4, 5. An increased concentration of IL-6, TNF-α, IL-2, IL-8, IL-1β, G-CSF, IL-17, GM- CSF, IL10, MCP1, MIP1α, chemokines, galectins in serum, was detected in COVID-19 patients 6-8. Interestingly, role of immunosuppressive cytokines, especially IL-10 may also contribute to COVID-19 induced damage of the lungs in latter stages of the disease9. Galectins are oligosaccharides chain complexes located on the cell surface and play an important role in regulating immune reactions and inflammatory responses10, 11, 12. Galectin-1 (Gal-1) was the first identified protein in this family. Even though it is known that Gal-1 has many regulatory functions in viral infections, to date, the role of Gal-1 in immunopathogenesis of the COVID-19 disease has not been clarified. Until now, only one study by Kazancioglu et al investigated potential role of galectins in SARS-CoV-2 induced inflammation. The results imply that levels of galectins (Gal-1 and Gal-3) are elevated in COVID-19 patients in comparison to healthy patients13. The aim of this study was to determine the association of Gal-1 and innate immunity cytokines such as IL-1β, IL-6, IL-10, IL-23, IL-33 with disease severity, alongside with clinical and biochemical features as well as CXR lung findings on disease severity in 210 patients with COVID-19. Results Clinical features of COVID-19 patients All of 210 patients included in the study met the criteria for COVID-1914 and were classified into the three groups according to disease severity. The median age of mild group (54. 9±1. 7) was significantly different from that of severe patients (59. 5±1. 3 ) or critical (61. 3±1. 4) (Table 1). Among all of them, 135 (57, 14%) were male. The analysis revealed a higher percentage of male patients and a lower percentage of female patients in group II and III with statistical significance (p = 0. 041; Table 1). The most frequent auscultatory finding in group I was attenuated breathing sound in 44. 3% of patients, with CXR findings described like interstitial thickening in 51. 4%, focal consolidation in 28. 5% and normal findings in 20%. In group II, 58. 5% of patients had auscultatory attenuated breathing sound, 48. 5% audible crackles diffusely with CXR findings described like multifocal consolidation in 44. 2% of hospitalized patients, focal consolidation in 30% and interstitial thickening in 25. 7%. In group III, auscultatory 90% of patients had audible crackles diffusely, attenuated breathing sound in 85. 7% with diffuse alveolar changes / ARDS on CXR findings. Only 10 (14. 2%) among this group had multifocal consolidation. Compared with mild group, the severe and critical group were more likely to have dyspnea, fatigue, auscultatory audible crackles diffusely. The percentage of patients with these findings was significantly higher than in the mild group (p < 0. 05; Table 1), which is in line with CXR findings of the patients. None of the patients in the severe and critical group had normal CRX finding, interstitial thickening or focal consolidation zones (CRX I, II, III), and none of the patients in the mild group had multifocal consolidation zones or diffuse alveolar changes / ARDS (CRX IV, V) (p=0. 000) (Table 1). In patients with severe and critical features, an increase in neutrophil count (p=0. 001), urea (p=0. 018), AST (p=0. 001), ALT (p=0. 001), BILD (p = 0. 018), CK (p=0. 012), LDH (p=0. 001), D-dimer (p=0. 001), CRP (p=0. 001), PCT (p=0. 001), Ferritin (p=0. 001) was noted, with reduced lymphocyte count (p=0. 001), monocyte count (p=0. 006), decreased levels of albumin (p=0. 001) and lower values of SaO2 (p=0. 001) and pO2 (p=0. 001) (Table 2). There were no statistically significant differences in white blood cell count, erythrocytes and platelets, + + hemoglobin; levels of plasma glucose, creatinine, BILT, K , Na , Fe, pH of blood, pCO2 (Table 2). Patients with the more severe form of COVID-19 had increased systemic values of Gal-1 Serum pro- and anti-inflammatory cytokines of interest were measured in COVID-19 patients. Systemic values of IL-1β (p=0. 001), IL-6 (p=0. 001), IL-10 (p=0. 026), IL-23 (p=0. 001), IL-33 (p=0. 001), and Gal-1 (p=0. 001) were significantly higher in stage III of COVID-19 patients compared to patients in stage I and II (Figure 1). Unchanged flow of ratios of Gal-1 and IL-10 with proinflammatory cytokines during COVID-19 development Analyses of the ratios of Gal-1 and IL-10 with IL-1β, IL-6, IL-23 and IL-33 were done. There were no statistically significant differences in the ratios of Gal-1/IL-1β, Gal-1/IL-6, Gal-1/IL-23, Gal-1/IL-33 and Gal-1/IL-10 as well as IL-10/IL-1β, IL-10/IL-6, IL-10/IL-23, IL-10/IL-33 between defined groups of COVID-19 patients (Figure 2).
Load more

Recommended publications
  • Ecologica URBO Kragujevac Ecologica URBO, Kragujevac ON
    ECOlogica URBO Kragujevac ECOlogica URBO, Kragujevac Agency for projecting, study and analysis making 3/1 Save Kovacevica 34000 Кragujevac fax: +381 (0) 34 337 237 E-mail: [email protected] ID number: 54931611 VAT: 101505921 Industry type: 74202 Account number: 220-19567-76 Pro Credit Bank STUDY ON ENVIRONMENTAL IMPACT ASSESSMENT OF THE PROJECT- RECONSTRUCTION OF PRODUCTION FACILITIES IN THE COMPANY “FIAT AUTOMOBILI SRBIJA” LOCATED AT THE CADASTRAL PARCEL. 1/1 KO KRAGUJEVAC 2, CITY OF KRAGUJEVAC PROJECT HOLDER “FIAT AUTOMOBILI SRBIJA” 4 Kosovska City of Kragujevac Kragujevac, October 2010 Study on environmental impact assessment of the Project-reconstruction of production facilities in the company “Fiat Automobili Srbija” located at the cadastral parcel no. 1/1 KO Kragujevac 2, City of Kragujevac ECOlogica URBO Kragujevac 2 PROJECT “FIAT AUTOMOBILI SRBIJA” HOLDER: 4 Kosovska City of Kragujevac STUDY Agency ECOlogica Urbo MADE BY: Kragujevac RESPONSIBLE Evica Rajic, ecologist PERSON: WORK Svetlana Djokovic, ecologist TEAM: Ivan Sindjelic, mechanical engineer Dr. Vladimir Simonovic, metallurgical engineer Sreto Lazarevic, building engineer Dragan Radovanovic, chemist Jelena Cekovic, biologist MA Ivan Cekovic, ecologist Jasmina Radovanovic, chemist Ivan Blagojevic, spatial planner Vesna Crnoglavac, lawyer Study on environmental impact assessment of the Project-reconstruction of production facilities in the company “Fiat Automobili Srbija” located at the cadastral parcel no. 1/1 KO Kragujevac 2, City of Kragujevac ECOlogica URBO Kragujevac 3 GENERAL DOCUMENTATION Study on environmental impact assessment of the Project-reconstruction of production facilities in the company “Fiat Automobili Srbija” located at the cadastral parcel no. 1/1 KO Kragujevac 2, City of Kragujevac ECOlogica URBO Kragujevac 4 STUDY BODY Study on environmental impact assessment of the Project-reconstruction of production facilities in the company “Fiat Automobili Srbija” located at the cadastral parcel no.
    [Show full text]
  • Support Measures for Learners in Higher Education
    Published on Eurydice (https://eacea.ec.europa.eu/national-policies/eurydice) Definition of the Target Group(s) Disabled persons studying in Serbian higher education institutions may receive financial support for studies. Students from socially disadvantaged families may receive social scholarships. Specific Support Measures Financial support for learners is defined by theLaw on Pupils and Students' Standard [1], whereby students are entitled to the following: 1. Accommodation 2. Subsidised meals 3. Student loans 4. Student grants 5. Grants for talented students Accommodation and subsidized meals The state subsidises accommodation and meals for all students at public institutions who are funded from the state budget. In addition, subsidised accommodation in dorms is granted to those studying out of their place of residence. There are 8 student centres in Serbia which provide both subsidised accommodation and meals (Belgrade, Novi Sad, Niš, Kragujevac, Subotica, Čačak, Bor and Užice). The city of Belgrade can accommodate 12,000 students in 14 dorms, Novi Sad 3,000 students etc. Students from private universities and those who are not funded from the state budget have the possibility of getting accommodation in a student dorm only on condition that there are vacant places left and at the economic prices. Approximately 7.36% of students were accommodated in dorms in the academic year 2011/12. In the same academic year, revenues collected from dorm accommodation fees were approximately 1.6 million EUR, whereas the state subsidised an additional amount of 8 million EUR. According to the EQUIED report, in the academic year 2011/12, public expenditures for subsidised accommodation and meals were 22 million EUR, whereas approximately 40,000 students were using subsidised meals.
    [Show full text]
  • Grad Adresa Naziv Lokala Bela Crkva Vuka Karadžića 4 Lacrima Beograd
    Pronađi lokal* sa spiska, pokaži svoj kod i tvoja Coca-Cola stiže! *Svakog ponedeljka lista lokala se dopunjuje Grad Adresa Naziv lokala Bela Crkva Vuka Karadžića 4 Lacrima Beograd Slavka Miljkovića 77-1 Sc Aeksandar Beograd Hajduk Veljka 30 Restoran Vinogradi doo Beograd Obilićev Venac 18 Creperie Haris Waffle Ice Cream Beograd Ada Ciganlija, Makiška Strana bb Bikini Bar Beograd Dr. Dragoslava Popovića 24 Bar Green House Beograd Balkanska 2 Coffee, Tea & Sympathy Beograd Paunova 80 Pavone Trattoria 11 Beograd Vojvode Bojovica Blue Wave Beograd Vidska 7 Caffe Feliks Beograd Gospodara Vucica 245 TheBoss Beograd Požeška 41 Informa 2013 Beograd Luke Lukalovića 8 Dbr Bar Beograd Požeška 118 Balance Gym Beograd Požeška 76 Sur Chicago Beograd Ada Ciganlija Desna Obala 4 Makondo Beograd Trgovačka 18 Corner Beer Beograd Majora Zorana Radosavljevića 246 Karamarkovic La Luna Beograd Palmotićeva 11 Parlament Point Beograd Partizanske avijacije 40 City cafe Beograd Omladinskih brigada 86 Laboratorija Beograd Goce Delčeva 2 Zero Zero Beograd Nedeljka Gvozdenovića 22 Cafe Dada Dia Beograd Jurija Gagarina 26 Contra bar Beograd Svetozara Markovića 4 Svetozar Beograd Jurija Gagarina 147 Dom Perigion Beograd Bulevar Zorana Đinđića 64a Kaldi Beograd Šumadijska 29 Placer cafe Beograd Njegoševa 53 Kafeterija Gardoš Beograd Borska 44 Kafe Connect Beograd Savski kej bb Crna maca Beograd Kej Oslobodjenja bb Monca Namare Beograd Makenzijeva 45 Ba Ba Lu Beograd Radomira Markovića 4 Geras Beograd Ada Ciganlija Desna Obala 13 Varadero Beograd Ada Ciganlija Makiška
    [Show full text]
  • Selo Knic, Gruza, Kragujevac
    Media Center Kragujevac Village Knic, Georgia, Kragujevac Telenet City Network | Serbia Phone: +38164 5558581; +38161 6154768; www.booking-hotels.biz [email protected] Village Knic, Georgia, Kragujevac Gruza je okruzena sa svih strana planinskim vencima Rudnika, Ramackih visova, Gledickih planina, Kotlenika i Jesevca, zimi je zasticena od udara jakih vetrova, a leti od velikih zega i toplote, te predstavlja pravu oazu za odmor i rekreaciju. Sa svojim plodnim ravnicama, niskim brezuljcima, negovanim vocnjacima, gostoljubivim stanovnistvom, bogatom kulturnom bastinom i istorijskim spomenicima, Gruza moze turistima da pruzi raznovrsnu i kvalitetnu ponudu. Centralni deo Gruze zauzima opstina Knic, sa 36 zivopisnih sela. Knic je od Beograda udaljen 142 km, preko Topole. Drumskim i zeleznickim saobracajem ima dobru vezu sa skoro svim krajevima Srbije. U sredisnjem delu opstine, u donjem toku reke Gruze, nalazi se jezero, koje po kolicinama i vrstama ribe spada u red bogatih jezera u Srbiji. Lovno podrucje Gruze, bogato raznim vrstama divljaci - zec, srna, fazan, jarebica, prepelica i divlja plovka - omogucuje organizovan lov. Od brojnih kulturno-istorijskih spomenika posebno se isticu ostaci srednjovekovnog grada, crkva iz 14. veka u Borcu i manastir Kamenac iz 15. veka u cestinu. Kragujevac Serbia The City of Kragujevac is the administrative, political, economic, educational and cultural hub of the District of Sumadija as well as the surrounding neighborhood Districts. The City is situated well, geographically. Kragujevac is in the center of Sumadija and Pomoravlje, 140 km to the south of the Capital City of Belgrade, by the highway E 10. Kragujevac covers an area of 835 square kilometers, andlies within the slopes of the Rudnik, Gledic and Crni Vrh Mountains.
    [Show full text]
  • Konačni Birački Spisak Članova Regionalnog Centra Kragujevac MS A
    Konačni birački spisak članova Regionalnog centra Kragujevac_MS_A Godina Regionalni Matična RB Ime (roditelj) Prezime Licenca, odnosno licence člana Komore Prebivalište mesto i opština rođenja centar sekcija 1 Aleksandar Rudnik (Dragoslav) Milanović 1967 300670104, 200084205, 100018211 Kragujevac, Kragujevac Kragujevac A 2 Aleksandar (Dragovan) Nedić 1981 400G18111, 300K66111 Aranđelovac, Aranđelovac Kragujevac A 3 Aleksandar (Dušan) Nenković 1954 400A98007, 300018103, 200140713 Kragujevac, Kragujevac Kragujevac A 4 Aleksandra (Velimir) Ristić 1965 300L16512 KRAGUJEVAC, Kragujevac Kragujevac A 5 Ana (Zoran) Virijević 1979 200136213 Aranđelovac, Aranđelovac Kragujevac A 6 Boban (M.) Milivojević 1963 300333603, 400302403, 200029203, 401L11918 Paraćin, Paraćin Kragujevac A 7 Bojan (Dušan) Stanojević 1979 300K94712, 400J87316, 381168717 Kragujevac, Kragujevac Kragujevac A 8 Branko (Dragan) Đoković 1982 300N03114, 401I31514, 301N24014 Aranđelovac, Aranđelovac Kragujevac A 9 Dejan (Lj.) Ilić 1969 200075504, 300148003, 400C65708, 381064113 Paracin, Paraćin Kragujevac A 10 Desanka (Rade) Pavlović 1963 300054403, 400B15207, 381122814 Kragujevac, Kragujevac Kragujevac A 11 Dragana (Dragan) Ristić 1975 400245703, 300395603, 200102408, 381116214 Cuprija, Ćuprija Kragujevac A 12 Dragana (Dragoljub) Macić 1971 300H93909, 381121614, 200155416 Paraćin, Paraćin Kragujevac A 13 Dragana (Ljubomir) Marinković 1982 400H31213, 300M55313, 381155316 Jagodina, Jagodina Kragujevac A 14 Dragana (N.) Biga 1964 200001503, 100005903, 300198103 ARANĐELOVAC, Aranđelovac
    [Show full text]
  • Council of Europe European Landscape Convention
    COUNCIL OF EUROPE EUROPEAN LANDSCAPE CONVENTION National Workshop on the implementation of the European Landscape Convention in Bosnia and Herzegovina Drawing landscape policies for the future Trebinje, Bosnia and Herzegovina 25-26 January 2018 SESSION 1 SERBIA Mrs Jasminka LUKOVIC JAGLICIC Director Advisor, Regional Economic Development Agency, Sumadija and Pomoravlje The role of the Regional Economic Development Agency for Sumadija and Pomoravlje in the process of the implementation of the European Landscape Convention at regional and local level The Regional Economic Development Agency for Sumadija and Pomoravlje was founded in 2002 as the partnership between public, civil and private sectors, with the purpose of planning and management of equal territorial development. The Law on Regional Development (adopted in July 2009, “Official Gazette of the Republic of Serbia”, No. 51/2009, 30/2010 and 89/2015) defined the competence and area of intervention of regional development agencies for planning of development processes at regional level, applying the principles of broad stakeholder participation, inter-municipal and cross-sector approach in identifying problems and measures to address them. REDASP consistently applies these principles in its work on the one hand and has the ratification of the European Landscape Convention on the other hand. Thus the Republic of Serbia has recognised the landscape as an essential component of the human environment and agreed to 1). establish and implement a set of policies aimed at the protection, management and planning of the area and 2). to establish procedures for involvement of the wider public, local and regional authorities, as well as other landscape policy stakeholders.
    [Show full text]
  • Serbia Emergency Energy Efficiency Program Summary of Energy Conservation Measures
    SERBIA EMERGENCY ENERGY EFFICIENCY PROGRAM SUMMARY OF ENERGY CONSERVATION MEASURES IMPLEMENTED BY c3 Nexanr IN FIVE SERBIAN MUNICIPALITIES DURING WINTER 2001 ON BEHALF OF THE UNITED STATES AGENCY FOR INTERNATIONAL DEVELOPMENT JUNE 2002 Contract No. LAG-1-00-98-00006-00 Task Order 109.008 SERBIA EMERGENCY ENERGY EFFICIENCY PROGRAM TABLE OF CONTENTS Page BACKGROUND ................................................................................................. I MUNICIPALITY OF SUBOTICA ........................................................................ 3 MUNICIPALITY OF ZRENJANIN .................................................................... 13 MUNICIPALITY OF KRAGUJEVAC................................................................ 18 MUNICIPALITY OF KRUSEVAC..................................................................... 25 MUNICIPALITY OF NOVl PAZAR ................................................................... 38 SERBIA EMERGENCY ENERGY EFFICIENCY PROGRAM - Background BACKGROUND The Re~ublicof Serbia, with Belgrade as capital, is wrt of the Federal Republic of Yugoslavia, and contains the autonomous provinces of ~ojvodina,and Kosovo and ~etohija.~ccording to the last available census data (19911, the total population is 9,779,000, of which 1,600,000 reside in Belgrade, the administrative; economic and cultural center of Serbia. Located in the central part of the Balkan Peninsula, on the most important route linking Europe and Asia, Serbia is referred to as the crossroads of Europe, occupying an area of 88,361
    [Show full text]
  • List of Hauliers Serbia 10 Oct 10
    Report on issued ECMT licences to Serbian hauliers for 2010 ECMT licences for EURO III safe lorries ECMT licence No Issued to haulier 1 Radivojev DOO,Vrbas 2 Yuba-Radomir Mišković i drugi o.d, Beograd 3 Astra SB d.o.o, Surčin-Beograd 4 Macko d.o.o, Odžaci 5 Marjanović Trans d.o.o, Futog 6 Marjanović Trans d.o.o, Futog 7 Silo Jeličić d.o.o,Požega 8 Silo Jeličić d.o.o,Požega 9 ISCO d.o.o, Zrenjanin 10 Banex Trans,Beograd 11 Teoma Transport d.o.o, Novi Beograd 12 Teoma Transport d.o.o, Novi Beograd 13 STS-Trans DOO,Kanjiža 14 Alex Internacional d.o.o, Niš 15 Bugarinović Transport d.o.o, Novi Sad 16 Bugarinović Transport d.o.o, Novi Sad 17 Braća Crnomarković,Stari Banovci 18 Braća Crnomarković,Stari Banovci 19 Braća Crnomarković,Stari Banovci 20 Popović Transport d.o.o, Obrovac 21 Dunis DOO,Futog 22 Dunis DOO,Futog 23 Dunis DOO,Futog 24 Dunis DOO,Futog 25 Dunis DOO,Futog 26 MB Transporte d.o.o, Malo Vojlovce-Lebane 27 NN Borović d.o.o, Ivanjica 28 Cvetković d.o.o, Novi Sad 29 Grade Trans d.o.o, Čačak 30 Trgo-Auto d.o.o, Srbobran 31 Unitrag Pižon,Beograd 32 Unitrag Pižon,Beograd 33 Srboexport Transport d.o.o,Obrenovac 34 Koncern Srboexport d.o.o Beograd, Obrenovac-Zabrežje 35 Koncern Srboexport d.o.o Beograd, Obrenovac-Zabrežje 36 Koncern Srboexport d.o.o Beograd, Obrenovac-Zabrežje 37 Koncern Srboexport d.o.o Beograd, Obrenovac-Zabrežje 38 Tim-Trade GVB d.o.o, Raška 39 Magazin-Transport d.o.o,Kruševac 40 Partnertrans,Novi Sad 41 Partnertrans,Novi Sad 42 Partnertrans,Novi Sad 43 Partnertrans,Novi Sad 44 Bata d.o.o,Trešnjevac 45 Bata d.o.o,Trešnjevac
    [Show full text]
  • Kriterijum Regionalni Centar Beograd Novi Sad Niš Subotica Kragujevac
    Kriterijum % od % od % od % od % od % od Ukupno članova koji ukupnog ukupnog ukupnog Broj članova sa 20 ili više ukupnog Broj članova sa 15 ili više ukupnog Broj članova koji su posetili bar ukupnog Ukupno Različitih nisu polaznici broja broja broja poena (nacionalni i broja poena po nacionalnom broja jedan događaj po evropskom broja članova polaznika predavanja (nacionalni članova članova članova evropski program) članova programu članova programu (imaju 5 ili više bodova) članova i evropski program) Regionalni centar Komore Komore Komore Komore Komore Komore Beograd 14567 52,8% 3437 12,5% 11130 40,3% 836 3,0% 1103 4,0% 758 2,7% Novi Sad 4173 15,1% 1237 4,5% 2936 10,6% 349 1,3% 490 1,8% 320 1,2% Niš 2907 10,5% 743 2,7% 2164 7,8% 220 0,8% 243 0,9% 198 0,7% Subotica 1247 4,5% 457 1,7% 790 2,9% 146 0,5% 210 0,8% 83 0,3% Kragujevac 1512 5,5% 450 1,6% 1062 3,8% 137 0,5% 183 0,7% 83 0,3% Kraljevo 1195 4,3% 389 1,4% 806 2,9% 110 0,4% 131 0,5% 84 0,3% Čačak 970 3,5% 299 1,1% 671 2,4% 80 0,3% 117 0,4% 59 0,2% Valjevo 919 3,3% 254 0,9% 665 2,4% 69 0,3% 77 0,3% 82 0,3% Kosovska Mitrovica 106 0,4% 17 0,1% 89 0,3% 0 0,0% 1 0,0% 1 0,0% Ukupno 27596 100,0% 7283 26,4% 20313 73,6% 1947 7,1% 2555 9,3% 1668 6,0% Održano predavanja 238 Predavanja iz nacionalnog 205 programa Predavanja iz evropskog 33 programa Podeljenih bodova 106533 Ukupno polaznika na svim 18645 predavanjima Po strukama (nacionalni i evropski program) Ukupno članova broj_polaznika Struka Ukupno članova broj_polaznika % 9533 Građevinci 9533 2288 24,0% Arhitekte 5656 1559 27,6% 5656 5293
    [Show full text]
  • Serbia 2Nd Periodical Report
    Strasbourg, 23 September 2010 MIN-LANG/PR (2010) 7 EUROPEAN CHARTER FOR REGIONAL OR MINORITY LANGUAGES Second periodical report presented to the Secretary General of the Council of Europe in accordance with Article 15 of the Charter SERBIA The Republic of Serbia The European Charter for Regional or Minority Languages The Second Periodical Report Submitted to the Secretary General of the Council of Europe Pursuant to Article 15 of the Charter Belgrade, September 2010 2 C O N T E N T S 1. INTRODUCTION ……………………………………………………………………6 2. Part I …………………………………………………………………………………12 2.1. Legislative and institutional changes after the first cycle of monitoring of the implementation of the Charter …………………………………………………….12 2.1.1. Legislative changes ……………………………………………………….12 2.1.2. The National Strategy for the Improvement of the Status of Roma ……..17 2.1.3. Judicial Reform …………………………………………………………...17 2.1.4. Establishment of the Ministry of Human and Minority Rights …………..23 2.2. Novelties expected during the next monitoring cycle of the implementation of the Charter …………………………………………………………………………….24 2.2.1. The Census ………………………………………………………………..24 2.2.2. Election of the national councils of the national minorities ……………...26 2.3. Implementation of the recommendations of the Committee of Ministers of the Council of Europe (RecChL(2009)2) 28) …………………………………………29 2.4. Activities for the implementation of the box-recommendation of the Committee of Experts with regard to the implementation of the Charter ………………………...33 3. PART II Implementation of Article 7 of the Charter ……………………………..38 3.1. Information on the policy, legislation and practice in the implementation of Part II - Article 7 of the Charter ……………………………………………………………..38 3.1.1.
    [Show full text]
  • THE RESULTS of STUDY: Mapping of Services for the Treatment of Adolescents with Substance Use Disorders
    THE RESULTS OF STUDY: Mapping of services for the treatment of adolescents with substance use disorders Authors: The National Expert Committee for Addiction Diseases; Republic of Serbia, Ministry of Health Prof. Michal Miovsky, Charles University in Prague Partners: United Nations Office on Drugs and Crime (UNODC) Serbia The Results of Study: Mapping of services for the treatment of adolescents with substance use disorders This report is an independent review of the consultants. The views expressed therein are those of the authors and do not necessarily represent the views of UNODC. Contents GLOSSARY OF TERMS ...........................................................................................................................................................4 INTRODUCTION ...................................................................................................................................................................... 5 EXECUTIVE SUMMARY .........................................................................................................................................................6 DRUG USE ................................................................................................................................................................................8 Prevalence and trends ..............................................................................................................................................................................8 High-risk drug use .......................................................................................................................................................................................8
    [Show full text]
  • Kragujevac J
    171 Kragujevac J. Sci. 29 (2007) 171-172. UDC IN MEMORIAM Vojislav Petrović, academician (1925-2007) Vojislav M. Petrović was born at Mala Kamenica, near Negotin, on January 2, 1925. His parents were teachers. He finished the Grammar school in Negotin in 1943, and hen he took an active part in National- liberation war against the enemies. After the war, he started biology studies at the Faculty of Mathematics and Science in Belgrade, and he graduated in 1952. He started to work as a trainee teacher at The Normal school and at the Grammar school at Negotin. At the beginning of 1956, he was elected member of the stuff at the Physiology Department at the Faculty of Mathematics and Science, Belgrade. Advised by professors Ivan Đaja and Stefan Đelineo, he centered his work on endocrinology studies and completed his doctorate dissertation" Endocrinal factors of thermoregulation and thermal adaptation" at The Faculty of Mathematics and Science, Belgrade University Remarkable results in his doctorate dissertation attracted the attention of Andre Mayer foundation and The French Academy of Science and he was awarded a scholarship and invited to join the research in the Laboratory for pharmacology at the College de France, and in the endocrinology laboratory of the Museum of Nature in Paris. After this period, he worked for a short period at the Institute of Physiology of the Faculty of Medicine in Strasbourg. He became a lecturer in Comparative physiology. The Canadian National Council for Scientific Research invited him, and he, together with his Canadian colleagues, worked, as a visiting scientist in the Biology Laboratory in Ottawa on the research in endocrinology and thermal adaptation.
    [Show full text]