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Ontario Species at Risk Evaluation Report for Tri-Colored Bat
Ontario Species at Risk Evaluation Report for Tri-colored Bat (Perimyotis subflavus) Committee on the Status of Species at Risk in Ontario (COSSARO) Assessed by COSSARO as Endangered June, 2015 Final Pipistrelle de l’Est (Perimyotis subflavus) La pipistrelle de l’Est (Perimyotis subflavus) est l’une des plus petites chauves-souris en Amérique du Nord. Environ 10 p. 100 de son aire de répartition mondiale se situe au Canada (en Ontario, au Québec, au Nouveau-Brunswick et en Nouvelle-Écosse) et elle est considérée rare dans la majeure partie de son aire de répartition canadienne. En Ontario, elle est considérée peu courante, bien que la taille des populations ne soit pas bien connue. La pipistrelle de l’Est se nourrit d’insectes. Elle s’alimente au-dessus de l’eau, le long des cours d’eau ainsi qu’à la lisière des forêts; elle évite généralement les grands champs ouverts ou les zones de coupe à blanc. À l’automne, les chauves-souris reviennent aux gîtes d’hibernation, qui peuvent être à des centaines de kilomètres de distance de leurs sites d’été. Elles s’agglutinent près de l’entrée, elles s’accouplent, puis elles pénètrent dans ce gîte d’hibernation ou elles se déplacent vers un gîte différent pour y passer l’hiver. La femelle produit un ou deux petits par année après l’âge d’un an et la longévité maximale consignée est de 15 ans. La principale menace qui pèse sur la pipistrelle de l’Est est une maladie appelée le syndrome du museau blanc (SMB), qui est causé par l’introduction du champignon Pseudogymnoascus destructans. -
Fungal Sampling of a Maternity Roost of Big Brown Bats (Eptesicus Fuscus) on the Baca National Wildlife Refuge
Fungal sampling of a maternity roost of Big Brown Bats (Eptesicus fuscus) on the Baca National Wildlife Refuge. Erin M Lehmer, Stephen Fenster & Kirk Navo Background The initial research was focused on sampling fungal community diversity on the migratory Mexican free-tailed bat (Tadarida brasiliensis) population from the Orient Mine upon arrival and prior to departure from Colorado. However, in June 2015 because of cold spring temperatures and higher than average precipitation, arrival of the free-tailed population was delayed, and we were unable to capture bats after repeated sampling efforts. Because of these failed efforts, it was decided to move to the nearby Baca National Wildlife Refuge in an attempt to capture resident (i.e. non-migratory) bats, using a stacked mist net system. During the single night of sampling at the Baca NWR, we captured 32 adult female big brown bats (Eptesicus fuscus) from a single maternity roost located in the attic of an abandoned outbuilding on the refuge property. These bats were processed in the same manner that we had processed the free-tailed bats in previous seasons; after capture, they were weighed, sex and reproductive condition were determined, and forearm lengths were measured. Fungal spores were collected by swabbing the wing membranes and dorsal and ventral fur with sterile cotton swabs dipped in sterile water. During routine processing of the fungal spores (i.e. culturing, PCR and DNA sequence barcoding analysis), we determined that 2 of the samples were a very close genetic match to P. destructans based on sequence alignment data of the internal transcribed spacer (ITS) region of the genome. -
<I>Geomyces Destructans</I> Sp. Nov. Associated with Bat White-Nose
MYCOTAXON Volume 108, pp. 147–154 April–June 2009 Geomyces destructans sp. nov. associated with bat white-nose syndrome A. Gargas1, M.T. Trest2, M. Christensen3 T.J. Volk4 & D.S. Blehert5* [email protected] Symbiology LLC Middleton, WI 53562 USA [email protected] Department of Botany, University of Wisconsin — Madison Birge Hall, 430 Lincoln Drive, Madison, WI 53706 USA [email protected] 1713 Frisch Road, Madison, WI 53711 USA [email protected] Department of Biology, University of Wisconsin — La Crosse 3024 Crowley Hall, La Crosse, WI 54601 USA [email protected] U.S. Geological Survey — National Wildlife Health Center 6006 Schroeder Road, Madison, WI 53711 USA Abstract — We describe and illustrate the new species Geomyces destructans. Bats infected with this fungus present with powdery conidia and hyphae on their muzzles, wing membranes, and/or pinnae, leading to description of the accompanying disease as white-nose syndrome, a cause of widespread mortality among hibernating bats in the northeastern US. Based on rRNA gene sequence (ITS and SSU) characters the fungus is placed in the genus Geomyces, yet its distinctive asymmetrically curved conidia are unlike those of any described Geomyces species. Key words — Ascomycota, Helotiales, Pseudogymnoascus, psychrophilic, systematics Introduction Bat white-nose syndrome (WNS) was first documented in a photograph taken at Howes Cave, 52 km west of Albany, NY USA during winter, 2006 (Blehert et al. 2009). As of March 2009, WNS has been confirmed by gross and histologic examination of bats at caves and mines in Massachusetts, New Jersey, Vermont, West Virginia, New Hampshire, Connecticut, Virginia, and Pennsylvania. -
25 Chrysosporium
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Universidade do Minho: RepositoriUM 25 Chrysosporium Dongyou Liu and R.R.M. Paterson contents 25.1 Introduction ..................................................................................................................................................................... 197 25.1.1 Classification and Morphology ............................................................................................................................ 197 25.1.2 Clinical Features .................................................................................................................................................. 198 25.1.3 Diagnosis ............................................................................................................................................................. 199 25.2 Methods ........................................................................................................................................................................... 199 25.2.1 Sample Preparation .............................................................................................................................................. 199 25.2.2 Detection Procedures ........................................................................................................................................... 199 25.3 Conclusion .......................................................................................................................................................................200 -
Biosafety Measures for Working with Pseudogymnoascus Destructans in the Laboratory and Use Or Storage of Potentially Contaminated Materials
Biosafety Measures for Working with Pseudogymnoascus destructans in the Laboratory and Use or Storage of Potentially Contaminated Materials The emergent disease of bats, white-nose syndrome (WNS), has caused one of the most precipitous declines documented among North American wildlife. This disease is caused by the recently described fungal pathogen Pseudogymnoascus (formerly Geomyces) destructans. Because of the grave threat this fungus presents to populations of hibernating bats, precautions must be taken when working with P. destructans in the laboratory to prevent accidental release of the fungus into the environment. The following guidelines apply to all members of the WNS Diagnostic Laboratory Network. Other laboratories that work with P. destructans or that maintain specimens that may harbor viable fungus may use these guidelines to develop appropriate institutional standards for preventing accidental release of a Biosafety Level-2 (BSL-2) fungal pathogen of animals. Based upon biological risk assessment, work in the laboratory with viable P. destructans should be restricted to BSL-2 or higher. Adherence to this guidance will ensure that in accordance with the manual Biosafety in Microbiological and Biomedical Laboratories (BMBL) 5th Edition, appropriate procedures, mechanical controls, and containment equipment are in place to facilitate biosecure work with the fungus and to prevent accidental release. Use of a biosafety cabinet. Fungi such as P. destructans readily produce aerosolizeable, environmentally resistant, and long-lived spores (reproductive structures). Therefore, all manipulations of potentially viable P. destructans (fungal cultures, carcasses, unfixed tissue samples, wing swabs, environmental samples, fungal tape lifts) in the laboratory should be restricted to a certified Class I or Class II biosafety cabinet. -
Ericaceae Root Associated Fungi Revealed by Culturing and Culture – Independent Molecular Methods
a Ericaceae root associated fungi revealed by culturing and culture – independent molecular methods. by Damian S. Bougoure BSc (Hons) Thesis submitted in accordance with the requirements for the degree of Doctor of Philosophy Centre for Horticulture and Plant Sciences University of Western Sydney February 2006 2 ACKNOWLEDGEMENTS Although I am credited with writing this thesis there is a multitude of people that have contributed to its completion in ways other than hitting the letters on a keyboard and I would like to thank them here. Firstly I’d like to thank my supervisor, Professor John Cairney, whose knowledge and guidance was invaluable in steering me along the PhD path. The timing of John’s ‘motivational chats’ was uncanny and his patience particularly, during the writing stage, seemed limitless at times. I’d also like to thank the Australian government for granting me an Australian Postgraduate Award (APA) scholarship, Paul Worden from Macquarie University and the staff from the Millennium Institute at Westmead Hospital for performing DNA sequencing and the National Parks and Wildlife Service of New South Wales and Environmental Protection agency of Queensland for permission to collect the Ericaceae plants. Thankyou to Mary Gandini from James Cook University for showing me the path to a Rhododendron lochiae population through the thick North Queenland rainforest. Without her help and I’d still be pointing the GPS at the sky. Thankyou to the other people in the lab studying mycorrhizas including Catherine Hitchcock, Susan Chambers, Adrienne Williams and particularly Brigitte Bastias with whom I shared an office. Everyone mentioned was generally just as willing as I was to talk about matters other than mycorrhizas. -
S41598-020-70375-6.Pdf
www.nature.com/scientificreports OPEN A common partitivirus infection in United States and Czech Republic isolates of bat white‑nose syndrome fungal pathogen Pseudogymnoascus destructans Ping Ren1,2*, Sunanda S. Rajkumar1,10, Tao Zhang3, Haixin Sui4,5, Paul S. Masters5,6, Natalia Martinkova 7, Alena Kubátová 8, Jiri Pikula 9, Sudha Chaturvedi1,5 & Vishnu Chaturvedi 1,5* The psychrophilic (cold‑loving) fungus Pseudogymnoascus destructans was discovered more than a decade ago to be the pathogen responsible for white-nose syndrome, an emerging disease of North American bats causing unprecedented population declines. The same species of fungus is found in Europe but without associated mortality in bats. We found P. destructans was infected with a mycovirus [named Pseudogymnoascus destructans partitivirus 1 (PdPV-1)]. The virus is bipartite, containing two double-stranded RNA (dsRNA) segments designated as dsRNA1 and dsRNA2. The cDNA sequences revealed that dsRNA1 dsRNA is 1,683 bp in length with an open reading frame (ORF) that encodes 539 amino acids (molecular mass of 62.7 kDa); dsRNA2 dsRNA is 1,524 bp in length with an ORF that encodes 434 amino acids (molecular mass of 46.9 kDa). The dsRNA1 ORF contains motifs representative of RNA-dependent RNA polymerase (RdRp), whereas the dsRNA2 ORF sequence showed homology with the putative capsid proteins (CPs) of mycoviruses. Phylogenetic analyses with PdPV-1 RdRp and CP sequences indicated that both segments constitute the genome of a novel virus in the family Partitiviridae. The purifed virions were isometric with an estimated diameter of 33 nm. Reverse transcription PCR (RT-PCR) and sequencing revealed that all US isolates and a subset of Czech Republic isolates of P. -
25 Chrysosporium
25 Chrysosporium Dongyou Liu and R.R.M. Paterson contents 25.1 Introduction ..................................................................................................................................................................... 197 25.1.1 Classification and Morphology ............................................................................................................................ 197 25.1.2 Clinical Features .................................................................................................................................................. 198 25.1.3 Diagnosis ............................................................................................................................................................. 199 25.2 Methods ........................................................................................................................................................................... 199 25.2.1 Sample Preparation .............................................................................................................................................. 199 25.2.2 Detection Procedures ........................................................................................................................................... 199 25.3 Conclusion .......................................................................................................................................................................200 References .................................................................................................................................................................................200 -
A New Species of Galactomyces and First Reports of Four Fungi on Wheat Roots in the United Kingdom
©Verlag Ferdinand Berger & Söhne Ges.m.b.H., Horn, Austria, download unter www.biologiezentrum.at A new species of Galactomyces and first reports of four fungi on wheat roots in the United Kingdom H. KwasÂna1 & G. L. Bateman2 1 Department of Forest Pathology, Agricultural University, ul. Wojska Polskiego 71c, 60-625, Poznan , Poland 2 Department of Plant Pathology and Microbiology, Rothamsted Research, Harpenden, Hertfordshire, AL5 2JQ, UK KwasÂna H. & Bateman G. (2007) A new species of Galactomyces and first reports of four fungi on wheat roots in the United Kingdom. Sydowia 60 (1): 69±92. A new species, Galactomyces britannicum (IMI395371, MycoBank 511261), is described from the roots of wheat in the UK. Dendryphion penicillatum var. sclerotiale, Fusariella indica, Pseudogymnoascus appendiculatus and Volucrispora graminea are reported for the first time from roots, rhizosphere or stem bases of wheat in the UK. A microconidiogenus synanamorph is described for V. graminea and the species is epitypified to reflect this amendment. Keywords: Dendryphion penicillatum var. sclerotiale, Fusariella indica, Galactomyces britannicum, Pseudogymnoascus appendiculatus, taxonomy, Volu- crispora graminea. The introduction of synthetic low nutrient agar (SNA; Nirenberg 1976) to induce fungal sporulation in Fusarium has proved invalu- able for the assessment of fungal diversity on or in the roots and stem bases of cereal plants (Bateman & KwasÂna 1999, Dawson & Bateman 2001a, b). The medium stimulates a fungal sporulation and allows the isolation of slow-growing species. Isolation studies on SNA have led to the recovery of new speciesof fungiand fungipre- viously unknown from cereal crops. This paper describes one new species and reports four rare spe- cies isolated from the roots, rhizosphere, or stem bases at soil level, of wheat grown in the UK. -
Mycoportal: Taxonomic Thesaurus
Mycoportal: Taxonomic Thesaurus Scott Thomas Bates, PhD Purdue University North Central Campus Eukaryota, Opisthokonta, Fungi chitinous cell wall absorptive nutrition apical growth-hyphae Eukaryota, Opisthokonta, Fungi Macrobe chitinous cell wall absorptive nutrition apical growth-hyphae Eukaryota, Opisthokonta, Fungi Macrobe Microbe chitinous cell wall absorptive nutrition apical growth-hyphae Primary decomposers in terrestrial systems Essential symbiotic partners of plants and animals Penicillium chrysogenum Saccharomyces cerevisiae Geomyces destructans Magnaporthe oryzae Pseudogymnoascus destructans “In 2013, an analysis of the phylogenetic relationship indicated that this fungus was more closely related to the genus Pseudogymnoascus than to the genus Geomyces changing its latin binomial to Pseudogymnoascus destructans.” Magnaporthe oryzae Pseudogymnoascus destructans Magnaporthe oryzae Pseudogymnoascus destructans Magnaporthe oryzae “The International Botanical Congress in Melbourne in July 2011 made a change in the International Code of Nomenclature for algae, fungi, and plants and adopted the principle "one fungus, one name.” Pseudogymnoascus destructans Magnaporthe oryzae Pseudogymnoascus destructans Pyricularia oryzae Pseudogymnoascus destructans Magnaporthe oryzae Pseudogymnoascus destructans (Blehert & Gargas) Minnis & D.L. Lindner Magnaporthe oryzae B.C. Couch Pseudogymnoascus destructans (Blehert & Gargas) Minnis & D.L. Lindner Fungi, Ascomycota, Ascomycetes, Myxotrichaceae, Pseudogymnoascus Magnaporthe oryzae B.C. Couch Fungi, Ascomycota, Pezizomycotina, Sordariomycetes, Sordariomycetidae, Magnaporthaceae, Magnaporthe Symbiota Taxonomic Thesaurus Pyricularia oryzae How can we keep taxonomic information up-to-date in the portal? Application Programming Interface (API) MiCC Team New Taxa/Updates Other workers Mycobank DB Page Views Mycoportal DB Mycobank API Monitoring for changes regular expression: /.*aceae THANKS!. -
Real-Time PCR for Geomyces Destructans Bat White-Nose Syndrome
In Press at Mycologia, preliminary version published on September 6, 2012 as doi:10.3852/12-242 Short title: Real-time PCR for Geomyces destructans Bat white-nose syndrome: a real-time TaqMan polymerase chain reaction test targeting the intergenic spacer region of Geomyces destructans Laura K. Muller US Geological Survey. National Wildlife Health Center, 6006 Schroeder Road, Madison, Wisconsin 53711 Jeffrey M. Lorch Molecular and Environmental Toxicology Center, University of Wisconsin at Madison, Medical Sciences Center, 1300 University Avenue, Madison, Wisconsin 53706 Daniel L. Lindner1 US Forest Service, Northern Research Station, Center for Forest Mycology Research, One Gifford Pinchot Drive, Madison, Wisconsin 53726 Michael O’Connor1 Wisconsin Veterinary Diagnostic Laboratory, 445 Easterday Lane, Madison, Wisconsin 53706 Andrea Gargas Symbiology LLC, Middleton, Wisconsin 53562 USA David S. Blehert2 US Geological Survey, National Wildlife Health Center, 6006 Schroeder Road, Madison, Wisconsin 53711 Copyright 2012 by The Mycological Society of America. Abstract: The fungus Geomyces destructans is the causative agent of white-nose syndrome (WNS), a disease that has killed millions of North American hibernating bats. We describe a real-time TaqMan PCR test that detects DNA from G. destructans by targeting a portion of the multicopy intergenic spacer region of the rRNA gene complex. The test is highly sensitive, consistently detecting as little as 3.3 fg genomic DNA from G. destructans. The real-time PCR test specifically amplified genomic DNA from G. destructans but did not amplify target sequence from 54 closely related fungal isolates (including 43 Geomyces spp. isolates) associated with bats. The test was qualified further by analyzing DNA extracted from 91 bat wing skin samples, and PCR results matched histopathology findings. -
Curriculum Vitae (PDF)
CURRICULUM VITAE Steven J. Taylor April 2020 Colorado Springs, Colorado 80903 [email protected] Cell: 217-714-2871 EDUCATION: Ph.D. in Zoology May 1996. Department of Zoology, Southern Illinois University, Carbondale, Illinois; Dr. J. E. McPherson, Chair. M.S. in Biology August 1987. Department of Biology, Texas A&M University, College Station, Texas; Dr. Merrill H. Sweet, Chair. B.A. with Distinction in Biology 1983. Hendrix College, Conway, Arkansas. PROFESSIONAL AFFILIATIONS: • Associate Research Professor, Colorado College (Fall 2017 – April 2020) • Research Associate, Zoology Department, Denver Museum of Nature & Science (January 1, 2018 – December 31, 2020) • Research Affiliate, Illinois Natural History Survey, Prairie Research Institute, University of Illinois at Urbana-Champaign (16 February 2018 – present) • Department of Entomology, University of Illinois at Urbana-Champaign (2005 – present) • Department of Animal Biology, University of Illinois at Urbana-Champaign (March 2016 – July 2017) • Program in Ecology, Evolution, and Conservation Biology (PEEC), School of Integrative Biology, University of Illinois at Urbana-Champaign (December 2011 – July 2017) • Department of Zoology, Southern Illinois University at Carbondale (2005 – July 2017) • Department of Natural Resources and Environmental Sciences, University of Illinois at Urbana- Champaign (2004 – 2007) PEER REVIEWED PUBLICATIONS: Swanson, D.R., S.W. Heads, S.J. Taylor, and Y. Wang. A new remarkably preserved fossil assassin bug (Insecta: Heteroptera: Reduviidae) from the Eocene Green River Formation of Colorado. Palaeontology or Papers in Palaeontology (Submitted 13 February 2020) Cable, A.B., J.M. O’Keefe, J.L. Deppe, T.C. Hohoff, S.J. Taylor, M.A. Davis. Habitat suitability and connectivity modeling reveal priority areas for Indiana bat (Myotis sodalis) conservation in a complex habitat mosaic.