Piwil1 Rabbit Polyclonal Antibody – TA327300 | Origene

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Piwil1 Rabbit Polyclonal Antibody – TA327300 | Origene

OriGene Technologies, Inc. 9620 Medical Center Drive, Ste 200 Rockville, MD 20850, US Phone: +1-888-267-4436 [email protected] EU: [email protected] CN: [email protected] Product datasheet for TA327300 Piwil1 Rabbit Polyclonal Antibody Product data: Product Type: Primary Antibodies Applications: WB Recommended Dilution: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:20-1:100; IP: 1:20-1:100 Reactivity: Mouse, Rat Host: Rabbit Isotype: IgG Clonality: Polyclonal Immunogen: Recombinant protein of mouse MIWI Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3 Concentration: lot specific Purification: Affinity purification Conjugation: Unconjugated Storage: Store at -20°C as received. Stability: Stable for 12 months from date of receipt. Gene Name: piwi-like RNA-mediated gene silencing 1 Database Link: NP_067286 Entrez Gene 363912 RatEntrez Gene 57749 Mouse Q9JMB7 Background: The Drosophila piwi gene was identified as being required for the self-renewal of germline stem cells. Piwi homologs are well conserved among various species including Arabidopsis, C. elegans, and Homo sapiens. Both Miwi and Mili proteins are mouse homologs of Piwi and contain a C-terminal Piwi domain. Miwi and Mili bind to Piwi-interacting RNAs (piRNAs) in male germ cells and are essential for spermatogenesis in mice. Synonyms: HIWI; MIWI; PIWI This product is to be used for laboratory only. Not for diagnostic or therapeutic use. View online » ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 1 / 2 Piwil1 Rabbit Polyclonal Antibody – TA327300 Product images: Western blot analysis of extracts of various cell lines, using MIWI antibody. This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 2 / 2.

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Expression Pattern of Small Nucleolar RNA Host Genes and Long Non-Coding RNA in X-Rays-Treated Lymphoblastoid Cells
Int. J. Mol. Sci. 2013, 14, 9099-9110; doi:10.3390/ijms14059099 OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 www.mdpi.com/journal/ijms Article Expression Pattern of Small Nucleolar RNA Host Genes and Long Non-Coding RNA in X-rays-Treated Lymphoblastoid Cells M. Ahmad Chaudhry Department of Medical Laboratory and Radiation Sciences, University of Vermont, Burlington, VT 05405, USA; E-Mail: [email protected]; Tel.: +1-802-656-0569; Fax: +1-802-656-2191 Received: 5 March 2013; in revised version: 19 April 2013 / Accepted: 22 April 2013 / Published: 25 April 2013 Abstract: A wide variety of biological effects are induced in cells that are exposed to ionizing radiation. The expression changes of coding mRNA and non-coding micro-RNA have been implicated in irradiated cells. The involvement of other classes of non-coding RNAs (ncRNA), such as small nucleolar RNAs (snoRNAs), long ncRNAs (lncRNAs), and PIWI-interacting RNAs (piRNAs) in cells recovering from radiation-induced damage has not been examined. Thus, we investigated whether these ncRNA were undergoing changes in cells exposed to ionizing radiation. The modulation of ncRNAs expression was determined in human TK6 (p53 positive) and WTK1 (p53 negative) cells. The snoRNA host genes SNHG1, SNHG6, and SNHG11 were induced in TK6 cells. In WTK1 cells, SNHG1 was induced but SNHG6, and SNHG11 were repressed. SNHG7 was repressed in TK6 cells and was upregulated in WTK1 cells. The lncRNA MALAT1 and SOX2OT were induced in both TK6 and WTK1 cells and SRA1 was induced in TK6 cells only. Interestingly, the MIAT and PIWIL1 were not expressed in TK6 cells before or after the ionizing radiation treatment.
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