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Identification of Lactobacillus Species Associated with Selected African Fermented Foods N Identification of Lactobacillus Species Associated with Selected African Fermented Foods N. A. Olasupo3, D. K. 01ukoyab an S. A. Odunfac a Department of Botany and Microbiology, Lagos State University Ojo, P. M. B. 1087 Apapa, Lagos, Nigeria b Genetics Division, National Institute for Medical Research, Yaba, Lagos, Nigeria c Department of Botany and Microbiology, University of Ibadan, Ibadan, Nigeria Z. Naturforsch. 52c, 105-108 (1997); received June 24/August 27, 1996 Lactobacillus, African Fermented Food Two hundred isolates of Lactobacillus were obtained from seven indigenous fermented foods namely: ’fufu’ (fermented cassava), 'iru' (fermented African locust bean), ’kenkey’ and ’ogi' (fermented maize), ’kunu-zarki’ (fermented millet), ’ugba’ (fermented African oil bean) and ’wara’ (fermented skimmed cow milk). Lactobacillus species identified were Lacto­ bacillus casei (17.0%), L. lactis (4.5%), L. plantarum (41.5%), L. brevis (11.5%), L. jensenii (5.5%), L. acidophilus (3.5%) L. cellobiosus (5.0%), L. delbrueckii (2.5%), L. salivarius (3.5%), L. leichmannii (3.5%) and L. fermentum (2.0%). The commonest species isolated was L. plantarum. This paper highlight the importance of Lactobacillus in the processing of African fermented foods. Introduction cies associated with our indigenous fermented Many African foods are fermented before con­ foods. sumption and the microorganisms involved in Af­ Materials and Methods rican foods fermentation are restricted to a few groups of yeasts and bacteria (Odunfa, 1985). Lac­ Source of organisms tic acid bacteria particularly Lactobacillus are in­ The Lactobacillus isolates were obtained from volved in the fermentation of many African stachy locally fermented foods. These were ’fufu’ (fer­ foods. Lactobacillus constitute a most important mented cassava), ’Kenkey’ (fermented maize), member within the lactic acid bacteria group and ’kunu-zarki’ (fermented millet), ’ogi’ (fermented is probably one of the most useful microorganisms maize), ’iru’ (fermented African locust bean), in food processing. Their activities and applica­ ’ugba’ (fermented African oil bean) and ’wara’ tions make possible the manufacture of many fer­ (fermented skimmed cow milk). mented foods, especially when used in singles or in mixed cultures with other types of bacteria Medium used for isolation (Chassy, 1985). In view of the widespread use of The medium used for the isolation of the lacto- Lactobacillus in the preparation of foods and bacilli was de Man Rogosa Sharpe (MRS) agar (de other fermentation processes (Mckay and Bal­ Man et al., 1960) containing per litre; beef extract, dwin, 1990) and the scantyness of information, 2g; tryptone, 10g; yeast extract, 4g; glucose, 10g; about the Lactobacillus species found in African K2H P 0 4.3H20, 2.5g; sodium acetate, 5g; tris am­ fermented foods. The previous information have monium citrate, 2g; MgS0 4.7H20, 200mg; manga- been geared towards gathering information on the nase sulphate, 50mg; Tween 80, 1ml and agar 15g. general lactic acid bacteria, yeasts and moulds. The medium was prepared according to the manu­ This study attempt to provide a detailed informa­ facturer’s direction and sterilized by autoclaving at tion about the different types of Lactobacillus spe- 121°C for 15min. Bacterial isolation and purification The food samples were prepared in the tradi­ Reprint requests to Dr. Olasupo. tional way following the methods of Odunfa 0939-5075/97/0100-0105 $ 06.00 © 1997 Verlag der Zeitschrift für Naturforschung. All rights reserved. D 106 N. A . O lasupo et al. ■ Lactoacillus Species Associated with African Fermented Foods (1985). One gram of each food item was weighed Results and Discussion into 9 ml of sterile 0.1% peptone water to form a Two hundred Lactobacillus isolates were ob­ pulp. Serial dilutions were than made from the tained from seven fermented foods sampled. Out pulp and plated on MRS agar using spread plate of a total 200 Lactobacillus isolates, 60 were ob­ method. Incubation was carried out at 37°C for tained from ’wara’ 48 from ’kenkey’, 36 from 48 hrs under anaerobic condition with the use of 'ugba,’ 30 from ’ogi\ 12 from ‘kunu-zarki’, 9 from disposable C0 2 and hydrogen generators (BBL, 'fufu’ while 5 isolates were obtained from 'iru\ Cockeysville, USA). In general, colonies repre­ The isolates were found to be L. casei, L. brevis, senting the square root of the number present L. jensenii, L. plantarum, L. leichmannii, L. del- were randomly selected from the plates obtained brueckii, L. cellobiosus, L. acidophilus, L. sali- from the highest countable dilution (Harrigan and varius, L. fermentum and L. lactis (Table I). McCance, 1976). The selected colonies were iso­ lated by streaking on MRS agar and incubated an­ aerobically under the same conditions as stated Table I: Identification of Lactobacillus isolates from se­ above. The purity of the isolated organisms was lected fermented foods. checked by streaking again on MRS agar plates, Fermented food Substrate Lactobacillus species followed by microscopic examination. Subse­ identified quently they were grown on slants of MRS agar 'Fufu' Cassava Lactobacillus casei stored at 5°C prior to identification. Lactobacillus brevis Lactobacillus jensenii Lactobacillus leichmannii Characterization and identification of organisms Lactobacillus plantarum Lactobacillus delbrueckii Pure cultures were subjected to physiological 'O gi’ Maize Lactobacillus plantarum and biochemical tests (Harrigan and McCance, Lactobacillus casei 1976; Sneath, 1986). Identification was based on Lactobacillus leichmannii Lactobacillus delbrueckii the following examinations^ 1) homolactic or het- Lactobacillus brevis erolactic character, determined by acetic acid or Lactobacillus jensenii ethanol production, ( 2) microscopic and macro­ 'Kunu-zarki' Millet Lactobacillus salivarius Lactobacillus casei scopic examination of morphology, (3) absence of Lactobacillus acidophilus catalase and oxidase, (4) absence of spore, (5) Lactobacillus jensenii Lactobacillus cellobiosus Gram stain reaction, ( 6) growth at 15° and 45°C, Lactobacillus plantarum and (7) fermentation of different carbon sources. 'Kenkey' Maize Lactobacillus plantarum Examination of results and identification of the Lactobacillus fermentum Lactobacillus brevis different strains were carried out using the Ber- Lactobacillus delbrueckii gey’s Manual of systematic Bacteriology (Sneath, Lactobacillus acidophilus Lactobacillus casei 1986). Lactobacillus cellobiosus API 50-CHL test kits (Bio Merieux,France) 'Iru' African locust bean Lactobacillus casei were employed to confirm the identity of the lac- Lactobacillus plantarum tobacilli (Cox and Thomsen, 1990). The test kits ’U gba’ African oil bean Lactobacillus plantarum enables the testing of the ability of the isolates to Lactobacillus casei Lactobacillus leichmannii ferment 49 carbohydrates in one test proceedure, Lactobacillus brevis; using the computer-aided identification Lactobacillus salivarius Lactobacillus jensenii programme. Lactobacillus acidophilus Lactobacillus cellobiosus Determination of percentage occurrence ’Wara’ Cow milk Lactobacillus casei Lactobacillus fermentum This was done according to the method used by Lactobacillus lactis Lactobacillus plantarum Hounhouigan et al. (1993.) Lactobacillus fermentum Lactobacillus jensenii Lactobacillus brevis Dominance species is Lactobacillus plantarum. N. A. Olasupo et al. ■ Lactoacillus Species Associated with African Fermented Foods 107 Among the organisms L. plantarum was found to strate and various physical factors. These condi­ be most commonly isolated species. Attempts tions allow for the development of a characteristic were made to isolate and characterize the different epiphytic flora, from which arises a population and types of Lactobacillus species found in assorted lo­ sequence of fermentation microorganisms when cally fermented foods in order (i) to have the the plant material is harvested and prepared for knowledge of the different species of Lactobacillus fermentation. involved in these fermentation processes, since the The lactobacilli constitute an important group substrates used in the preparation of these foods of organisms particularly in the food processing are different and (ii) to determine which species industry. The reasons for the wide spread use of can be improved in order to develop appropriate Lactobacillus in the preparation of foods and starter cultures for use in fermentation of local other fermentation processes are (i) production of foods. desired flavour or physical property such as ap­ Lactobacillus plantarum, L. brevis and L. casei pearance and texture in food, (ii) retardation of were the commonest Lactobacillus species iso- spoilage and reduction of contamination through latled. As in the earlier reports (Etchells et al., the production of antimicrobial substances, (iii) 1975; Olukoya et al, 1993) on the occurrence of enhancement of nutritional values of foods e.g. by lactic bacteria spectrum, L. plantarum constituted providing vitamins, amino acids and (iv) beneficial the highest number of Lactobacillus species iso­ effects on human health (Chassy, 1987; Sandine, lated from fermented plant mateials. The involve­ 1987; Mckay and Baldwin, 1990). ment of various types of Lactobacillus species in The lactobacillus have been implicated in the fermented vegatable and plant materials had natural fermentation of many local foods, for ex­ earlier been reported (Cooke et al., 1987; Steink- ample they have been isolated from fermented
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