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IMMUNOLOGY Exploring Chemokine Signaling LUNARIS™ Chemokine Kits Human 10-Plex: CCL2 (MCP-1), CCL3, CCL4, CCL5 (RANTES), CCL11 (Eotaxin), CCL20, CXCL1, CXCL8 (IL-8), CXCL10 (IP-10), CXCL12 (SDF1A) Mouse 11-Plex: CCL2 (MCP-1), CCL3, CCL4, CCL5 (RANTES), CCL11 (Eotaxin), CCL19, CCL20, CCL22, CXCL1, CXCL10 (IP-10), CX3CL1 (Fractalkine) Chemokines Chemokine receptor Plasma membrane its | 2020.01 | 09075 | www.vierviertel.com | 09075 | 2020.01 its K Cytoplasm AYOXXA Chemokine AYOXXA Intracellular signaling Chemotaxis www.ayoxxa.com LUNARIS™ Human & Mouse Chemokine Kits Validated, scalable, robust Validated for quantitative analysis of up to 12 different chemokines in one sample Profile homeostatic and proinflammatory chemokine signatures in sample volumes as low as 3 µL Scalable and standardized assay architecture with robust chemistry guarantees reproducibility Harmonized panels for human and murine samples allow rapid translation of results from lab to clinic LUNARIS™ Human 10-Plex Chemokine Kit Quantifies CCL2 (MCP-1), CCL3, CCL4, CCL5 (RANTES), CCL11 (Eotaxin), CCL20, CXCL1, CXCL8 (IL-8), CXCL10 (IP-10), CXCL12 (SDF1A) in serum and cell culture supernatant. LUNARIS™ Mouse 11-Plex Chemokine Kit Quantifies CCL2 (MCP-1), CCL3, CCL4, CCL5 (RANTES), CCL11 (Eotaxin), CCL19, CCL20, CCL22, CXCL1, LUNARIS™ Human 10-Plex CXCL10 (IP-10), CX3CL1 (Fractalkine) Chemokine Kit in serum and cell culture supernatant. For Research Use Only. Not for use in diagnostic procedures. Chemokine Signaling Chemokines constitute the largest family of cytokines and mediate versatile and powerful signaling in cell migration, Analytes contained in LUNARIS™ Chemokine Kits homeostasis, inflammation, angiogenesis, embryogenesis, grouped by cellular function cancer metastasis and several other medically relevant functions. The four classes of chemokines initiate complex signaling cascades by binding to specific 7-transmembrane G protein- Lymphocyte Mononuclear Granulocyte coupled receptors. traffic cell traffic traffic Building fine-tuned concentration gradients, chemokines exert precise control of diverse regulatory pathways and cause pleiotropic effects that extend far beyond their originally described role as leucocyte chemoattractants. A plethora of literature bears witness to the known importance of these small cytokines in a vast number of biological functions, while a growing number of publications hints at a yet undescribed complex interplay between target cells and their respective Th1, Th2, Th17 cells Macrophages, Eosinophils, chemokines. natural killer cells neutrophils Chemokine repertoires of some signaling axes are extensively conserved between mice and humans, making murine models the system of choice in more than just immunology contexts. CCL20, CCL22, CCL2, CCL3, CCL4, CCL11, CXCL1, CX3CL1, CXCL10 CCL5, CCL19, CX3CL1 CXCL8 (IL-8) Relevant research areas: Inflammation and infection biology Note: CXCL12 is mainly involved in bone marrow homing Allergy and autoimmune disease (e.g., retention of granulocytes, B cells and monocyte precursors). Hematopoiesis and angiogenesis www.ayoxxa.com 2 Excellent Data Quality Validation of LUNARIS™ Human 10-Plex Chemokine Kit* Table 1: Performance Analyte LoD LLOQ ULOQ DR Figure 1: Performance CCL2 Median assay parameters Bar diagram depicting the CCL2 0.2 0.3 200 2.3 CCL3 from 20 experiments. CCL3 4.6 12.0 300 2.5 median assay parameters CCL4 L0D = Limit of detection, CCL4 2.9 4.8 600 2.8 shown in table 1. The orange CCL5 LLOQ = Lower limit of CCL5 1.4 8.0 1000 3.0 bars represent the dynamic CCL11 quantification, CCL11 2.3 7.2 900 3.0 range of the assay from LoD CCL20 Analytes ULOQ = Upper limit CCL20 5.1 24.0 600 2.8 (left border) to the ULOQ CXCl1 CXCL1 9.2 40.0 1000 3.0 Analytes CXCL8 of quantification, (right border). The LLOQ is CXCL10 CXCL8 0.3 0.5 300 2.5 DR = Logarithmic detection depicted as a white line. CXCL12 CXCL10 4.9 8.0 3000 3.5 range. All values reported Measurements from 20 1 1 0 CXCL12 24.2 120.0 3000 3.5 10 0. 10 1000 0000 in pg/mL. validation experiments. 1 Cytokine concentration [pg/mL] Cytokine concentration [pg/mL ] Figure 2: Accuracy Figure 3: Precision 200 40 Median recovery rate Between-run precision (BRP) from 10 experiments of QC samples spiked into cell 150 30 quantifying markers in %) culture supernatants. Each data y( quality control samples point represents the median %) 100 P( 20 ver spiked at two different between-run precision of (%) BRP BR eco Recovery (%) concentrations into cell R 50 two different QC sample 10 culture supernatants. concentrations measured in The targeted recovery 0 10 experiments. The targeted 0 3 4 5 0 2 2 3 4 5 1 0 2 range (70–130%) is 2 11 20 L1 L8 precision (<20%) is denoted 11 20 L L8 CL CL CL C C L1 L1 CL CL CL CL C L1 L1 CL C C CL CL X X C C C C C C CL CL XC X C C C C C C C X X C C C C X X denoted by the dotted C C C by the dotted lines/grey C C lines/grey shaded area. shaded area. *Validation data of the corresponding mouse kit are available upon request. Innovative technology for Multiple options to access translational proteomics the advantages of LUNARIS™ Ease of readout and handling Complete testing and readout services Fully integrated system Send in samples for testing and receive a complete analysis report Flexible scalability from low to high throughput Perform assays in your lab, then send in Readout of 384 samples in less than one hour completed assay plates for readout AYOXXA’s proprietary LUNARIS™ platform for multiplex protein Innovative panel development analysis is a fully integrated and scalable system. A dedicated Custom panel configuration reader automates image-based analysis of immunoassay beads in a planar array, so every bead is read and every bead Custom multiplex assay development counts. LUNARIS™ is optimized for sample volumes as low as 3 µL – one-tenth the volume required for similar technologies – yet allows full multiplex testing without compromising data quality or precision from precious samples. With advantages of superior data quality, workflow flexibility and conservation of precious samples, LUNARIS™ enables reliable quantification of biomarkers from model to man – from lab to clinic – from data to insight. www.ayoxxa.com 3 AYOXXA’s innovative multiplex protein technology for translational proteomics Ordering information AYOXXA Biosystems is dedicated to the vision of enabling success in translational LUNARIS™ Kits No. of Cat. No. No. of BioChips samples * research. Human 10-Plex 1 × 96 LHCK-20100S 40 Chemokine Kit 4 × 96 LHCK-20100F 184 Building upon an innovative technology Mouse 11-Plex 1 × 96 LMCK-20110S 40 Chemokine Kit 4 × 96 LMCK-20110F 184 platform, our mission is to develop robust * Measured in duplicate assay panels for translational research applications. Our LUNARIS™ multiplex protein analysis platform is optimized for translating knowledge from basic to clinical research. With its advantages in terms of quality, flexibility and efficiency, its | 2020.01 | 09075 | www.vierviertel.com | 09075 | 2020.01 its LUNARIS™ enables reliable quantification K of biomarkers from model to man – from Exploring chemokine signaling lab to clinic – from data to insight. Call the experts: AYOXXA Chemokine AYOXXA +49 (0)221-222 529-0 HEADQUARTERS GERMANY USA [email protected] AYOXXA Biosystems GmbH AYOXXA Biosystems Inc. BioCampus Cologne c/o Platinum CFO Nattermannallee 1 2600 Tower Oaks Blvd., Suite 220 www.ayoxxa.com 50829 Köln, Germany Rockville, MD 20852 Phone: +49 (0) 221 222 529-0 USA E-Mail: [email protected] E-Mail: [email protected] www.ayoxxa.com 4.
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