US 20170042958A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2017/0042958 A1 Du et al. (43) Pub. Date: Feb. 16, 2017

(54) METHODS OF REDUCING SKIN 46R 8/97 (2006.01) NFLAMMATION USING COMPOSITIONS A61O 19/08 (2006.01) COMPRISING A6IR 36/48 (2006.01) GROSSEDENTATA AND ALBIZA JULIBRISSN EXTRACTS A6IR 9/00 (2006.01) (52) U.S. Cl. (71) Applicant: Johnson & Johnson Consumer Inc., CPC ...... A61K 36/87 (2013.01); A61K 36/48 Skillman, NJ (US) (2013.01); A61K 9/0014 (2013.01); A61K 8/97 (72) Inventors: Hua Du, Shanghai (CN); Yan Li, (2013.01); A61O 19/08 (2013.01); A61O Shanghai (CN); Tao Liu, Shanghai 19/007 (2013.01) (CN); Yingxin Ma, Shanghai (CN) (57) ABSTRACT (21) Appl. No.: 14/863,629 (22) Filed: Sep. 24, 2015 A composition for applying to skin in need of treatment for skin barrier restoration, hydration and moisturization, the (30) Foreign Application Priority Data composition comprising extracts of Ampelopsis grosseden tata and Albizia julibrissin. Also provided are methods for Aug. 14, 2015 (CN) ...... 2O15105OO612.1 treating skin in need of skin barrier treatment, methods of Publication Classification treating skin to reduce the signs of aging and methods of (51) Int. Cl. treating skin to reduce the signs of inflammation with a A6 IK 36/87 (2006.01) composition comprising extracts of Ampelopsis grosseden A61O 19/00 (2006.01) tata and Albizia julibrissin. US 2017/0042958 A1 Feb. 16, 2017

METHODS OF REDUCING SKN activity of flavonoid-rich extracts from leaves of Ampelopsis NFLAMMATION USING COMPOSITIONS grossedentata. J. Food Biochem. 2009, 33, 808-820.). Mod COMPRISING AMPELOPSIS ern pharmacology studies have shown that it had activities GROSSEDENTATA AND ALBIZA Such as anticancer, liver protection, anti-oxidation, anti JULIBRISSN EXTRACTS inflammatory and so on (Xiao J. Zheng, Hao Xiao, Zhi Zeng, ZiW. Sun, Can Lei, Jing Z. Dong, Ying Wang. Composition CROSS REFERENCE TO RELATED and serum antioxidation of the main flavonoids from fer APPLICATION mented vine tea (Ampelopsis grossedentata) has also been 0001. This application claims priority of the benefits of shown. Journal of functional foods. 2 0 1 4, 9, 290–294.) Chinese Patent Application No. 201510500612.1, filed Aug. 0005 Albizia julibrissin is a species of tree from the 14, 2015, the complete disclosure of which is hereby incor Fabaceae family, native to Southwestern and eastern Asia. It porated herein by reference in its entirety. is known by a wide variety of common names such as “Persian silk tree' or "pink sins”. It is widely planted as an FIELD OF THE INVENTION ornamental in parks and gardens. The seeds of the plant are used as food for livestock and by wildlife. The extracts 0002 The present invention relates to compositions for of Albizia julibrissin are found to have anti-depressant treating the skin barrier and improving skin hydration com properties Kim, J H; Kim, SY; Lee, SY; Jang, C G (2007). prising plant extracts for use on skin. More specifically, the “Antidepressant-like effects of Albizzia julibrissin in mice: present invention relates to compositions comprising Involvement of the 5-HT1A receptor system’. Pharmacol extracts of Ampelopsis grossedentata and Albizia julibrissin ogy, Biochemistry, and Behavior 87 (1): 41-7. In traditional for improving the condition and appearance of the skin, Such Chinese medicine, Albizia julibrissin is used to nourish the as by improving skin barrier protection, improving hydra heart and calm the spirit. See http://www.acupuncture-and tion and moisturization of the skin and reducing inflamma chinese-medicine.com/albizza.html, accessed Jun. 15, 2015. tion of the skin, and providing anti-aging properties to the It is also used for treating insomnia, injuries due to falls and skin. removing carbuncles. “Shen Nong's Classic', a famous Chinese ancient book, teaches its medicinal use for BACKGROUND OF THE INVENTION mental diseases. Albizia julibrissin main constituents 0003) A wide variety of compositions intended for include triterpenoid saponins, lignanoids, phenolic glyco improving the appearance of skin, including improving skin sides, flavonoids, spermine macrocyclic alkaloids and so on. hydration and moisturization, are known in art. However, Among them, the triterpenoid Saponins have been proved to most of these moisturizing agents work either through be the main bioactive principles of this crude drug (Kokila formation of occlusive films on the skin that prevent tran K. Priyadharshini S. D. Sujatha V. Phytopharmacological Sepidermal water loss (e.g., mineral oil, petrolatum and other properties of Albizia species: a review. Int J Pharm Pharm oils), or through humectants that attract and retain moisture Sci 2013; 5(Suppl. 3):70-3.). Modern pharmacology studies on the skin Surface (e. g. glycerin, propylene glycol, buty have shown it possessed sedative, antidepressant, anti-oxi lene glycol and so on). There is a need for agents that protect dant, anti-tumor, immunomodulatory, anti-fertility and anti the skin barrier more holistically. platelet activating factor receptor activities. (Hongxiang 0004 Ampelopsis grossedentata or “Moyeam' is a spe Sun, Shuwang He, Minghua Shi. Adjuvant-active fraction cies of plant belonging to the family, genus: from Albizia julibrissin Saponins improves immune Ampelopsis Michx. The specific name grossedentata comes responses by inducing cytokine and chemokine at the site of from the plant's growing structure and leaves. It is mainly injection. International Immunopharmacology 22 (2014) distributed in middle and south of . It also finds 346-355). presence in Some South-east Asian countries. Moyeam is 0006. The skin provides a vital barrier structure that generally grown in Zhang Jiajie, the famous mountain in protects human from environmental insults. The epidermis, province of China. The leaves and stems are used to where most of the skin barrier function resides, is highly make a popular by the name “Moyearm in China. stratified and has an outermost layer that is cornified. Epi The tea is very popular among health-conscious consumers dermal barrier integrity disruption or dysfunction is patho for its high levels of flavonoids especially Dihydromyrice logically involved in a variety of compromised skin condi tin. Traditional uses include cure for respiratory diseases, tions, including dry skin, skin sensitization, atopic eliminating inflammation, as an anti-oxidant, regulating dermatitis, psoriasis and aging. To build a strong and blood Sugar and fat and as an anti-cancer agent. Ampelopsis improved skin barrier it is necessary to increase the thresh grossedentata is widely used as a medicinal plant. Its dried old to defend against extrinsic stimulates. Even for compro leaves and stems, also named as Vine Tea or Mao Yan Mei, mised skin conditions, the restoration of the skin barrier is have been consumed as a health tea and for also important for recovering and reducing appearance of hundreds of years. The chemical content of this plant dry, aging, inflammation and other pathological properties. includes flavonoids such as dihydromyricetin and myricetin (Du, Q.: Chen, P.; Jerz, G.; Winterhalter, P. Preparative 0007 Dry skin is a common skin condition that affects separation of flavonoid glycosides in leaves extract of almost all of people at Some times in their lives. Dry skin can Ampelopsis grossedentata using high-speed counter-current cause many skin problems including atopic dermatitis, chromatography. J. Chromatogr. A 2004, 1040, 147-149.). It eczema, psoriasis and pruritus. The moisturizing is also a was used as folk medicine or a daily drink for the treatment key step for an elastic and transparent skin condition, which of hepatitis, flu, hypertension, hyperglycemic and Sore makes skin look healthy, young and beautiful. throat. (Gao, J. H.; Liu, B. G.; Ning, Z. X. Zhao, R. X. 0008 Inflammatory skin conditions such as atopic der Zhang, A. Y.; Wu, Q. Characterization and antioxidant matitis, eczema and sensitive skin also have a close con US 2017/0042958 A1 Feb. 16, 2017 nection with the skin's barrier properties. Epidermal integ during epidermal differentiation. This function is catalyzed rity is necessary for the skin to defend against external by transglutaminase 1 (TGM 1). Most TGM1 is anchored to stimulants. the keratinocyte plasma membrane via fatty acyl linkages 0009 Filaggrin is an important protein involved in skin and can also function to crosslink lipids to the envelope. moisturizing and immune responses through its contribution Richard L. Eckert, Michael T Sturniolo, Ann-Marie to skin barrier structure and functions. Filaggrin can be Broome, Monica Ruse and Ellen A Rorke (2005)' Trans degraded to free amino acids forming a major component of glutaminase Function in Epidermis’ Journal of Investigative natural moisturizing factor (NMF), which serves as the Dermatology (2005) 124, 481-492 Mutation or reduced primary humectant of the stratum corneum (SC). 2-pyrroli amount of TGM 1 is associated with lamellar ichthyosis and done-5-carboxylic acid (PCA) and urocanic acid (UCA) are other skin problems with skin barrier dysfunction. Thus an two important NMF components, which bind water and increase of TGM 1 is beneficial for improving skin strength make the Surface of normal skin soft and flexible. Filaggrin, and preventing SC from coming off. together with NMF, contribute to stratum corneum (SC) 0013 Dry skin, aging, itching and other skin problems hydration and pH. Filaggrin is different from traditional are related to decreased differential ability and metabolism moisturizing ingredients which function to increase water of skin. Cell vitality is a basic source of skin physiological absorption. Filaggrin and NMF function to improve the skin properties. Recovery or improvement of skin cell vitality barrier function and water binding capability of the epider can provide a potential differential ability and metabolism of mis. Decreased filaggrin and NMF are mainly the key skin, which are important for skin regeneration and defend factors resulting in dry skin. Restoration or improvement of ing intrinsic and extrinsic stimulate. Accordingly, it would filaggrin and NMF is important for healthy and lively skin. be desirable to find active ingredients to promote filaggrin In addition, UCA may have several other functions in SC, and LOR to improve skin barrier. including UV photoprotection and as a scavenger of UV 0014 Applicants have surprisingly discovered that cer generated hydroxyl radicals Jacob P. Thyssen and Sanja tain extracts of Ampelopsis grossedentata when combined Kezic (2014) “Causes of epidermal filaggrin reduction and with certain extracts of Albizia julibrissin showed unex their role in the pathogenesis of atopic dermatitis. JAllergy pected synergy in providing Superior skin barrier protection, Clin Immunol 2014; 134:792-9.. reducing the signs of aging in the skin and reducing skin 0010 Additionally, filaggrin is also important for the inflammation. formation of the corneocyte. Filaggrin functions as an inter mediate filament-associated protein (IFAP) to aggregate SUMMARY OF THE INVENTION keratin filaments into macrofibrils, the largest filament 0015. Accordingly, in one aspect, the present invention is bundles present in corneocytes. Along with several other directed to topical compositions comprising extracts of epidermal differentiation-linked proteins, filaggrin is then Ampelopsis grossedentata and Albizia julibrissin wherein cross-linked into the cornified envelope. Filaggrin contrib the extracts of Ampelopsis grossedentata and the Albizia utes a strong structure that is a defense line for skin. Studies julibrissin are present in a weight ratio of 1:10 to 10:1 and have strongly suggested that perturbation of skin barrier a cosmetically acceptable topical carrier. function as a result of reduction or complete loss of filaggrin 0016. In another aspect, the present invention is directed expression leads to enhanced percutaneous transfer of aller to methods of treating skin in need of improving skin barrier gens, further stimulation of langerhans cells which lead to function with a topical composition comprising extracts of Th2 immune responses. This ultimately results in the pathol Ampelopsis grossedentata and Albizia julibrissin. ogy of skin inflammation. Improvement of filaggrin can 0017. In yet another aspect, the present invention is prevent or limit the attenuation of skin inflammation. Fil directed to methods of treating skin inflammation by apply aggrin is therefore in the frontline of defense, and protects ing to a skin in need of reducing skin inflammation a topical the body from the entry of foreign environmental substances composition comprising extracts of Ampelopsis grosseden that can otherwise trigger aberrant immune responses tata and Albizia julibrissin. Osawa R, Akiyama M, Shimizu H (2011). The filaggrin 0018. In yet another aspect, the present invention is gene defects and the risk of developing allergic disorders.” directed to methods of treating skin aging by applying to a Allergol Int. 2011 March; 60(1):1-9.). skin in need of improving signs of aging a topical compo 0011. The function and appearance of the skin may sition comprising extracts of Ampelopsis grossedentata and decline with age. This decline can be caused by skin barrier Albizia julibrissin. dysfunction. Loricrin (LOR) is another important protein that facilitates terminal differentiation of the epidermis and 0019. These and other features and advantages of the formation of the skin barrier. Human LOR is an insoluble present invention will be readily apparent from the follow protein initially expressed in the granular layer of the ing detailed description of the invention. epidermis during cornification and comprises 80% of the DETAILED DESCRIPTION OF THE total protein mass of the cornified envelope (CE). The INVENTION content of LOR reduces with age Mark Rinnerthaler, Jutta Duschl, Peter Steinbacher, Manuel Salzmann, Johannes Bis 0020 All percentages listed herein, unless otherwise chof (2013) Age-related changes in the composition of the stated, are weight percentages based on the total weight of cornified envelope in human skin'. Experimental Derma the composition. tology, 2013, 22, 329-335. It is therefore advantageous to 0021. As used herein, “skin in need of improving skin promote the expression of LOR to reduce the effects of aging barrier function' means, without limitation, skin that is on the skin. lacking in moisture, lacking in sebum, cracked, dry, itchy, 0012 LOR, involucrin and filaggrin are cross-linked by scaly, Xerodermic, dehydrated, skin that looks rough, peely, the formation of e-(g-glutamyl)lysine isodipeptide bonds irritated or inflamed; skin that is painful, itchy, lacks Supple US 2017/0042958 A1 Feb. 16, 2017

ness, lacks radiance, dull or lacks lipids, has altered free 0027. As used herein, “treatment of external aggressions fatty acids:ceramides:cholesterol ratio, has altered transepi in skin' means the reduction or prevention of the damage dermal water loss, has altered water barrier function, has from external aggressions in skin. Examples of external altered skin conductance, epidermal differentiation, aggressions include, but are not limited to, damage to the increased inflammation/irritation, hyperkeratinization, skin from the use of cleansers (e.g., topical cleansers con abnormal descquamation and bacterial proliferation, sensitive taining Surfactants), make-up, shaving as well as environ skin, compromised skin conditions like eczematic skin, skin mental damage Such as from UV light (e.g., Sun damage with dermatoses Such as atopic dermatosis, psoriasis, or from Sunlight or damage from non-natural sources Such as combinations of two or more thereof. UV lamps and Solar simulators), OZone, exhaust, pollution, 0022. As used herein, “skin in need of reducing skin chlorine and chlorine containing compounds, and cigarette inflammation” means a skin exhibiting redness or erythema, Smoke. Effects of external aggressions on the skin include, edema, or being reactive or sensitive to external elements. but are not limited to, oxidative and/or nitrosative damage to External elements include, but are not limited to, Sun rays and modifications on lipids, carbohydrates, peptides, pro (UV, visible, IR), microorganisms, atmospheric pollutants teins, nucleic acids, and . Effects of external aggres Such as OZone, exhaust pollutants, chlorine and chlorine sions on the skin also include, but are not limited to, loss of generating compounds, cigarette Smoke, cold temperature, cell viability, loss or alteration of cell functions, and changes heat, Soaps and detergents, cosmetics, jewelry. Inflammatory in gene and/or protein expression. disorders and related conditions which may be treated or 0028. As used herein, “improving the skin tone” means prevented by use of the compositions of this invention the lightening of the appearance of the skin (e.g., lightening include, but are not limited to the following: arthritis, pigmented marks or lesions, reducing skin sallowness, and/ bronchitis, contact dermatitis, atopic dermatitis, psoriasis, or evening the color of the skin). Seborrheic dermatitis, Sumac and poison oak dermatitis, 0029. As used herein, "cosmetically/dermatologically eczema, allergic dermatitis, polymorphous light eruptions, acceptable” means Suitable for use in contact with tissues inflammatory dermatoses, folliculitis, alopecia, poison ivy, (e.g., the skin or hair) without undue toxicity, incompatibil insect bites, acne inflammation, irritation induced by extrin ity, instability, irritation, allergic response, and the like. sic factors including, but not limited to, chemicals, trauma, 0030. As used herein, the term “safe and effective pollutants (such as cigarette Smoke) and Sun exposure, amount’ means an amount Sufficient to induce the desired secondary conditions resulting from inflammation including effect, but low enough to avoid serious side effects. The safe but not limited to Xerosis, hyperkeratosis, pruritus, post and effective amount of the compound, extract, or compo inflammatory hyperpigmentation, Scarring and the like. sition will vary with, e.g., the age, health and environmental Preferably, the inflammatory disorders and related condi exposure of the end user, the duration and nature of the tions which may be treated or prevented using the methods treatment, the specific extract, ingredient, or composition of the invention are arthritis, inflammatory dermatoses, employed, the particular pharmaceutically-acceptable car contact dermatitis, allergic dermatitis, atopic dermatitis, rier utilized, and like factors. polymorphous light eruptions, irritation, including erythema 0031. To provide a more concise description, some of the induced by extrinsic factors, acne inflammation, psoriasis, quantitative expressions given herein are not qualified with Seborrheic dermatitis, eczema, poison ivy, poison oak, poi the term “about'. It is understood that whether the term son Sumac, insect bites, folliculitus, alopecia, and secondary "about is used explicitly or not, every quantity given herein conditions and the like. is meant to refer to the actual given value, and it is also 0023. As used herein, “skin in need of improving or meant to refer to the approximation to such given value that reducing the signs of aging” means a skin that is, but not would reasonably be inferred based on the ordinary skill in limited to, sagging, loose, lax, rough, wrinkly, thinned, and the art, including approximations due to the experimental uneven. Improving the signs of aging means improving the and/or measurement conditions for Such given value. firmness of the skin, improving the texture of the skin, 0032 To provide a more concise description, some of the improving the appearance of wrinkles in skin, improving the quantitative expressions herein are recited as a range from skin tone or the treatment of external aggressions in skin. about amount X to about amount Y. It is understood that 0024. As used herein, “improving the firmness of skin' wherein a range is recited, the range is not limited to the means the enhancing of the firmness or elasticity of the skin, recited upper and lower bounds, but rather includes the full preventing the loss of firmness or elasticity of skin, or range from about amount X through about amount Y, or any preventing or treating sagging, lax and loose skin. The amount or range therein. firmness or elasticity of the skin can be measured by use of 0033. As described herein, applicants have discovered a cutometer. See Handbook of Non-Invasive Methods and that compositions comprising extracts of Ampelopsis the Skin, eds. J. Serup, G. Jemec & G. Grove, Chapter 66.1 grossedentata and Albizia julibrissin provide unexpectedly (2006). The loss of skin elasticity or firmness may be a result good skin barrier function, and reduce, inhibit, treat, and of a number of factors, including but not limited to aging, delay any signs of skin barrier function impairment. In environmental damage, or the result of an application of a particular, applicants have discovered that extracts of cosmetic to the skin. Ampelopsis grossedentata and Albizia julibrissin induce 0025. As used herein, “improving the texture of skin' promotion of natural moisturizing factors by the skin itself. means the Smoothing of the Surface of the skin to remove and Such induced production of natural moisturizing factors either bumps or crevasses on the skin Surface. will have a beneficial effect in improving skin barrier 0026. As used herein, “improving the appearance of function and skin hydration and moisturization. wrinkles in skin' means preventing, retarding, arresting, or 0034. According to one aspect of the present invention, reversing the process of wrinkle and fine line formation in compositions comprising extracts of and extracts of the skin. flowers of Albizia julibrissin when combined in a weight US 2017/0042958 A1 Feb. 16, 2017

ratio of 1:10 to 10:1 provide a significant increase in the thereof having a dielectric constant value of between 1 and natural moisturizing factors of the skin of a human being. 100, preferably between 4 and 60, and more preferably 0035 Ampelopsis grossedentata Extract: between 5 and 40 at 20° C., including, but not limited to, 0036) Any suitable manner of preparing the extracts of those solvents and combinations of solvents having the Ampelopsis grossedentata for use in accordance with the desired dielectric constant value as disclosed in "Dielectric present invention may be used. Suitable extracts may be Constants of Some Organic Solvent-Water Mixtures at Vari obtained using conventional methods including, but not ous Temperatures.” Akerlof, Gosta; JACS, Vol. 54, No. 11 limited to, direct extraction from the biomass by grinding, (November 1932), pp. 4125-4139, incorporated herein by macerating, pressing. Squeezing, mashing, centrifuging, reference. In certain preferred embodiments, the polar and/or processes Such as cold percolation, agitation/distilla extract is extracted using one or more C-Cs alcohols, C-Cs tion, microwave assisted extraction, Sonication, Supercriti polyols, C-C glycols, and combinations of two or more cal/subcritical CO compressed gas extraction with or with thereof. In certain more preferred embodiments, the extract out polarity modifier, pressurized solvent extraction, is extracted using one or more C-C alcohols, C-C poly accelerated solvent extraction, Surfactant assisted pressur ols, and/or C-C glycols. In certain more preferred embodi ized hot water extraction, oil extraction, membrane extrac ments, the extract is prepared using a solvent comprising tion, Soxhlet extraction, the gold finger distillation/extrac methanol, ethanol, or a combination thereof with or without tion and/or processes disclosed, for example, in U.S. Pat. presence of water. In certain preferred embodiments, the Nos. 7,442,391, 7,473.435, and 7,537,791 to Integrated extract may be further refined by charcoal (also referred to Botanical Technologies, LLC, incorporated herein by refer as active carbon) treatment. ence, and the like, or by other methods such as solvent 0039. In certain preferred embodiments, the extract of the extraction, and the like. In particular, an extract in accor invention is an extract prepared by pulverizing the Ampelop dance with the present invention preferably is a solvent sis grossedentata leaves and extracting using a solvent based extraction made by grinding or macerating plant having a dielectric constant of a value between about 1 and material in a solvent, typically an organic solvent Such as an about 80 at 20°C., preferably a dielectric constant of a value alcohol, acetone, liquid carbon dioxide with or without between about 2 and about 60 at 20° C., more preferably a polarity modifier, hexane, or chloroform. The resulting dielectric constant of a value between about 2 and about 40 extract comprised mainly non-polar compounds. The plant at 20° C., and even more preferably a dielectric constant of biomass preferably is separated entirely from the extraction, a value between about 2 and 35 at 20° C. and is not used after extraction. 0040. In certain embodiments, the composition may addi 0037. Any of a variety of solvents including polar sol tionally include extracts from other parts of an Ampelopsis vents, non-polar solvents, or combinations of two or more grossedentata plant for example, one or more of the stem, thereof may be used in methods of comprising solvent bark, , fruits, seeds, or flowers. In other embodiments, extraction. Preferably, polar solvents are used. Suitable polar the composition is essentially free from extracts of other Solvents include polar inorganic solvents such as water and non-leaf parts of Ampelopsis grossedentata plant. the like, polar organic Solvents such as alcohols and corre 0041. In certain embodiments, the composition may com sponding organic acids, for example C-C alcohols includ prise extracts from cell cultures of of Ampelopsis ing methanol, ethanol, propanol, butanol, and the like and grossedentata. organic acids, including acetic acid, formic acid, propanoic 0042 Any Suitable amount of Ampelopsis grossedentata acid, and the like, polyols and glycols, including C-Cs extract may be used in the topical compositions of the polyols/glycols and the like, and combinations of two or present invention. In certain preferred embodiments, the more thereof. Suitable non-polar solvents include non-polar compositions comprise from greater than Zero to about 20% organic solvents such as alkanes, including C-Cs alkanes, Ampelopsis grossedentata extract. In certain other preferred cycloalkanes, including C-C alkanes, alkyl ethers, includ embodiments, the compositions comprise from about 0.0001 ing C-C alkyl ethers, Petroleum ethers, ketones, including to about 20%, from about 0.001 to about 10%, from about C-C ketones, methylene chloride, ethyl acetate, Xylene, 0.01 to about 5%, from about 0.1 to about 5%, or from about toluene, chloroform, vegetable oil, mineral oil and the like. 0.2 to about 2% Ampelopsis grossedentata extract. In certain In another embodiment extraction may be obtained by other preferred embodiments, the compositions comprise non-polar solvents described above or supercritical fluid from greater than Zero to about 1%, from about 0.0001 to extraction with or without a polar modifier Such as C-Cs about 1%, from about 0.001 to about 1%, or from about 0.01 alcohols, water, C-C polyols/glycols or C-C organic to about 1% Ampelopsis grossedentata extract. In certain acids. other preferred embodiments, the compositions comprise 0038. In certain preferred embodiments, the extract is a from about 1 to about 5%, preferably from about 2 to about polar extract. Polar extract means the extract is produced by 5% Ampelopsis grossedentata extract. In certain preferred Subjecting the plant or parts of the plant to a polar solvent. embodiments, the amounts of the Ampelopsis grossedentata In a certain embodiment, the extract is prepared by pulver extract are from the leaf of Ampelopsis grossedentata. izing the leaves of Ampelopsis grossedentata and extracting 0043 Albizia julibrissin Extract: using a polar solvent having a dielectric constant value of 0044 Any suitable manner of preparing the extracts of between 1 and 100 at 20°C., preferably a dielectric constant Albizia julibrissin for use in accordance with the present of a value between 4 and 60 at 20° C., more preferably a invention may be used. Suitable extracts may be obtained dielectric constant of a value between 4 and 50 at 20°C., and using conventional methods including, but not limited to, even more preferably a dielectric constant of a value direct extraction from the biomass by grinding, macerating, between 4 and 40 at 20° C. Examples of preferred polar pressing, Squeezing, mashing, centrifuging, and/or processes Solvents include C-C alcohols, C-C polyols/glycols, Such as cold percolation, agitation/distillation, microwave C-Cs organic acids, water and combinations of two or more assisted extraction, Sonication, Supercritical/subcritical CO US 2017/0042958 A1 Feb. 16, 2017

compressed gas extraction with or without polarity modifier, is extracted using one or more C-C alcohols, C-C poly pressurized solvent extraction, accelerated solvent extrac ols, and/or C-C glycols. In certain more preferred embodi tion, Surfactant assisted pressurized hot water extraction, oil ments, the extract is prepared using a solvent comprising extraction, membrane extraction, Soxhlet extraction, the methanol, ethanol, or a combination thereof with or without gold finger distillation/extraction and/or processes dis presence of water. In certain preferred embodiments, the closed, for example, in U.S. Pat. Nos. 7,442,391, 7473.435, extract may be further refined by charcoal (also referred to and 7,537,791 to Integrated Botanical Technologies, LLC, as active carbon) treatment. incorporated herein by reference, and the like, or by other 0047. In certain preferred embodiments, the extract of the methods such as solvent extraction, and the like. In particu invention is an extract prepared by pulverizing the Albizia lar, an extract in accordance with the present invention julibrissin flowers and extracting using a solvent having a preferably is a solvent-based extraction made by grinding or dielectric constant of a value between about 1 and about 80 macerating plant material in a solvent, typically an organic at 20°C., preferably a dielectric constant of a value between Solvent such as an alcohol, acetone, liquid carbon dioxide about 2 and about 60 at 20° C., more preferably a dielectric with or without polarity modifier, hexane, or chloroform. constant of a value between about 2 and about 40 at 20°C., The resulting extract comprised mainly non-polar com and even more preferably a dielectric constant of a value pounds. The plant biomass preferably is separated entirely between about 2 and 35 at 20° C. from the extraction, and is not used after extraction. 0048. In certain embodiments, the composition may addi 0045 Any of a variety of solvents including polar sol tionally include extracts from other parts of an Albizia vents, non-polar solvents, or combinations of two or more julibrissin tree for example, one or more of the stem, bark, thereof may be used in methods of comprising solvent wood, roots, leaves, fruits, or seeds. In other embodiments, extraction. Preferably, polar solvents are used. Suitable polar the composition is essentially free from extracts of other Solvents include polar inorganic solvents such as water and non-flower parts of Albizia julibrissin tree. the like, polar organic Solvents such as alcohols and corre 0049. In certain embodiments, the composition may com sponding organic acids, for example C-C alcohols includ prise extracts from cell cultures of trees of Albizia julibris ing methanol, ethanol, propanol, butanol, and the like and Sin. organic acids, including acetic acid, formic acid, propanoic 0050. Any suitable amount of Albizia julibrissin extract acid, and the like, polyols and glycols, including C-Cs may be used in the topical compositions of the present polyols/glycols and the like, and combinations of two or invention. In certain preferred embodiments, the composi more thereof. Suitable non-polar solvents include non-polar tions comprise from greater than Zero to about 20% Albizia organic solvents such as alkanes, including C-Cs alkanes, julibrissin extract. In certain other preferred embodiments, cycloalkanes, including C-C alkanes, alkyl ethers, includ the compositions comprise from about 0.0001 to about 20%, ing C-C alkyl ethers, Petroleum ethers, ketones, including from about 0.001 to about 10%, from about 0.01 to about C-C ketones, methylene chloride, ethyl acetate, Xylene, 5%, from about 0.1 to about 5%, or from about 0.2 to about toluene, chloroform, vegetable oil, mineral oil and the like. 2% Albizia julibrissin extract. In certain other preferred In another embodiment extraction may be obtained by embodiments, the compositions comprise from greater than non-polar solvents described above or supercritical fluid Zero to about 1%, from about 0.0001 to about 1%, from extraction with or without a polar modifier Such as C-Cs about 0.001 to about 1%, or from about 0.01 to about 1% alcohols, water, C-C polyols/glycols or C-C organic Albizia julibrissin extract. In certain other preferred embodi acids. ments, the compositions comprise from about 1 to about 5%, 0046. In certain preferred embodiments, the extract is a preferably from about 2 to about 5% Albizia julibrissin polar extract. Polar extract means the extract is produced by extract. In certain preferred embodiments, the amounts of Subjecting the plant or parts of the plant to a polar solvent. the Albizia julibrissin extract are from the flower of Albizia In a certain embodiment, the extract is prepared by pulver julibrissin. izing the flowers of Albizia julibrissin and extracting using 0051. When the topical compositions containing the a polar solvent having a dielectric constant value of between extracts according to the present invention are applied to the 1 and 100 at 20° C., preferably a dielectric constant of a skin it is believed only a certain percentage of the extracts value between 4 and 60 at 20° C., more preferably a according to the present invention penetrates the epidermis. dielectric constant of a value between 4 and 50 at 20°C., and The penetration percentage is determined by a lot of factors, even more preferably a dielectric constant of a value such as molecule weight, solubility etc., with very broad between 4 and 40 at 20° C. Examples of preferred polar variation. The present invention further comprises a method solvents include C-C alcohols, C-C polyols/glycols, of improving the barrier function and improving hydration C-Cs organic acids, water and combinations of two or more and moisturization of the skin by applying to skin in need of thereof having a dielectric constant value of between 1 and improving skin barrier function and improving skin mois 100, preferably between 4 and 60, and more preferably turization a composition comprising extracts of Ampelopsis between 5 and 40 at 20° C., including, but not limited to, grossedentata and Albizia julibrissin, in particular a combi those solvents and combinations of solvents having the nation of extracts of Ampelopsis grossedentata leaves and desired dielectric constant value as disclosed in "Dielectric Albizia julibrissin flowers in a weight ratio ranging from Constants of Some Organic Solvent-Water Mixtures at Vari 1:10 to 10:1. The method comprises for example topically ous Temperatures.” Akerlof, Gosta; JACS, Vol. 54, No. 11 applying a composition of the present invention comprising (November 1932), pp. 4125-4139, incorporated herein by extracts of Ampelopsis grossedentata and Albizia julibrissin, reference. In certain preferred embodiments, the polar in particular a combination of extracts of Ampelopsis extract is extracted using one or more C-Cs alcohols, C-Cs grossedentata leaves and Albizia julibrissin flowers in a polyols, C-Cs glycols, and combinations of two or more weight ratio ranging from 1:10 to 10:1, to skin in need of thereof. In certain more preferred embodiments, the extract improving skin barrier function. Such topical application US 2017/0042958 A1 Feb. 16, 2017

may be to any skin in need of treatment on the body, for of Ampelopsis grossedentata and Albizia julibrissin. In example skin of the face, lips, neck, chest, back, arms, particular, the amounts of the extracts of Ampelopsis buttocks, axilla, and/or legs. Preferably, the extracts are grossedentata and Albizia julibrissin to be used preferably is polar extracts of Ampelopsis grossedentata and Albizia selected to achieve the desired treatment of a given skin julibrissin. condition. The extract of Ampelopsis grossedentata and the 0052. The present invention further comprises a method extract of Albizia julibrissin are present in the extract of reducing skin dryness by applying to skin in need a composition in amounts from a weight ratio, by weight of composition comprising extracts of Ampelopsis grosseden the extract composition of about 1:10 to about 10:1, pref tata and Albizia julibrissin, in particular a combination of erably from about 1:7 to about 7:1, more preferably from extracts of Ampelopsis grossedentata leaves and Albizia about 1:5 to about 5:1, even more preferably from about 1:3 julibrissin flowers in a weight ratio ranging from 1:10 to to about 3:1 and even more preferably from about a ratio of 10:1. The method comprises for example topically applying 1:1. a composition of the present invention comprising extracts 0056. Any suitable carrier may be used in the composi of Ampelopsis grossedentata and Albizia julibrissin, in par tions. Preferably, the carrier is a cosmetically-acceptable ticular a combination of extracts of Ampelopsis grosseden carrier. As will be recognized by those of skill in the art, tata leaves and Albizia julibrissin flowers in a weight ratio cosmetically acceptable carriers comprise carriers that are ranging from 1:10 to 10:1, to skin in need of reducing skin suitable for use in contact with the body, in particular the inflammation. Such topical application may be to any skin in skin, without undue toxicity, incompatibility, instability, need of treatment on the body, for example skin of the face, irritation, allergic response, and the like. A safe and effective lips, neck, chest, back, buttocks, arms, axilla, and/or legs. amount of carrier is from about 50% to about 99.999%, Preferably, the extracts are polar extracts of Ampelopsis preferably from about 80% to about 99.9%, more preferably grossedentata and Albizia julibrissin. from about 99.9% to about 95%, most preferably from about 0053. The present invention further comprises a method 98% to about 99.8% by weight of the composition. of reducing skin inflammation by applying to skin in need of 0057 The carrier can be in a wide variety of forms. For reducing skin inflammation a composition comprising example, carriers in the form of emulsions, including, but extracts of Ampelopsis grossedentata and Albizia julibrissin not limited to, oil-in-water, water-in-oil, water-in-oil-in in an inflammation-reducing therapeutically effect amount. water, and oil-in-water-in-silicone emulsions, are useful In particular, the method uses a combination of extracts of herein. These emulsions can cover a broad range of viscosi Ampelopsis grossedentata leaves and Albizia julibrissin ties, e.g., from about 100 cps to about 200,000 cps using a flowers in a weight ratio ranging from 1:10 to 10:1. The Brookfield RVT viscometer. method comprises for example topically applying a compo 0.058 Examples of suitable cosmetically-acceptable car sition of the present invention comprising extracts of riers include cosmetically acceptable solvents and materials Ampelopsis grossedentata and Albizia julibrissin, in particu for cosmetic solutions, Suspensions, lotions, creams, serums, lar a combination of extracts of Ampelopsis grossedentata essences, gels, toners, Sticks, sprays, ointments, liquid leaves and Albizia julibrissin flowers in a weight ratio washes and Soap bars, shampoos, hair conditioners, pastes, ranging from 1:10 to 10:1, to skin in need of reducing skin foams, mousses, powders, shaving creams, wipes, patches, inflammation. Such topical application may be to any skin in strips, powered patches, micro-needle patches, bandages, need of treatment on the body, for example skin of the face, hydrogels, film-forming products, facial and skin masks, lips, neck, chest, back, buttocks, arms, axilla, and/or legs. make-up, liquid drops, and the like. These product types Preferably, the extracts are polar extracts of Ampelopsis may contain several types of cosmetically-acceptable carri grossedentata and Albizia julibrissin. ers including, but not limited to solutions, Suspensions, 0054 The present invention further comprises a method emulsions such as micro-emulsions and nano-emulsions, of improving skin aging by applying to skin in need of gels, Solids, liposomes, other encapsulation technologies and improving skin aging a composition comprising extracts of the like. Ampelopsis grossedentata and Albizia julibrissin in a thera 0059. The following are non-limiting examples of carri peutically effective amount for reducing a sign of aging. In ers. Other carriers can be formulated by those of ordinary particular the method uses a combination of extracts of skill in the art. In one embodiment, the carrier contains Ampelopsis grossedentata leaves and Albizia julibrissin water. In a further embodiment, the carrier may also contain flowers in a weight ratio ranging from 1:10 to 10:1. The one or more aqueous or organic solvents. Examples of method comprises for example topically applying a compo organic solvents include, but are not limited to: dimethyl sition of the present invention comprising extracts of isosorbide; isopropylmyristate; surfactants of cationic, Ampelopsis grossedentata and Albizia julibrissin, in particu anionic and nonionic nature; vegetable oils; mineral oils; lar a combination of extracts of Ampelopsis grossedentata waxes; gums; synthetic and natural gelling agents; alkanols; leaves and Albizia julibrissin flowers in a weight ratio glycols; and polyols. Examples of glycols include, but are ranging from 1:10 to 10:1, to skin in need of improving skin not limited to, glycerin, propylene glycol, butylene glycol, aging. Such topical application may be to any skin in need pentalene glycol, hexylene glycol, polyethylene glycol, of treatment on the body, for example skin of the face, lips, polypropylene glycol, diethylene glycol, triethylene glycol, neck, chest, back, buttocks, arms, axilla, and/or legs. Pref capryl glycol, glycerol, butanediol and hexanetriol, and erably, the extracts are polar extracts of Ampelopsis grossed copolymers or mixtures thereof. Examples of alkanols entata and Albizia julibrissin. include, but are not limited to, those having from about 2 0055 Any suitable amount of extracts of Ampelopsis carbon atoms to about 12 carbon atoms (e.g., from about 2 grossedentata and Albizia julibrissin may be used in the carbon atoms to about 4 carbon atoms). Such as isopropanol compositions of the present invention. Preferably, the com and ethanol. Examples of polyols include, but are not limited positions comprise a safe and effective amounts of extracts to, those having from about 2 carbon atoms to about 15 US 2017/0042958 A1 Feb. 16, 2017

carbon atoms (e.g., from about 2 carbon atoms to about 10 0067. The compositions of the present invention can also carbon atoms) such as propylene glycol. The organic Sol be formulated into a solid formulation (e.g., a wax-based vents may be present in the carrier in an amount, based upon Stick, Soap bar composition, powder, or wipe). The compo the total weight of the carrier, of from about 1 percent to sition of the present invention can also be combined with a about 99.99 percent (e.g., from about 20 percent to about 50 Solid, semi-solid, or dissolvable Substrate (e.g., a wipe, percent). Water may be present in the carrier (prior to use) mask, pad, glove, or strip). in an amount, based upon the total weight of the carrier, of 0068. The compositions of the present invention may from about 5 percent to about 95 percent (e.g., from about further comprise any of a variety of additional cosmetically 50 percent to about 90 percent). Solutions may contain any active agents. Examples of Suitable additional active agents suitable amounts of solvent, including from about 40 to include: skin lightening agents, darkening agents, additional about 99.99%. Certain preferred solutions contain from anti-aging agents, tropoelastin promoters, collagen promot about 50 to about 99.9%, from about 60 to about 99%, from ers, anti-acne agents, shine control agents, antimicrobial about 70 to about 99%, from about 80 to about 99%, or from agents such as anti-yeast agents, anti-fungal, and anti about 90 to 99% of Solvent. bacterial agents, anti-inflammatory agents, anti-parasite 0060 Alotion can be made from such a solution. Lotions agents, external analgesics, Sunscreens, photoprotectors, typically contain at least one emollient in addition to a antioxidants, keratolytic agents, detergents/surfactants, solvent. Lotions may comprise from about 1% to about 20% moisturizers, nutrients, vitamins, energy enhancers, anti (e.g., from about 5% to about 10%) of an emollient(s) and perspiration agents, astringents, deodorants, hair removers, from about 50% to about 90% (e.g., from about 60% to hair growth enhancing agents, hair growth delaying agents, about 80%) of water. firming agents, hydration boosters, efficacy boosters, anti 0061 Another type of product that may be formulated callous agents, agents for skin conditioning, anti-cellulite from a solution is a cream. A cream typically contains from agents, odor-control agents such as odor masking or pH about 5% to about 50% (e.g., from about 10% to about 20%) changing agents, and the like. of an emollient(s) and from about 45% to about 85% (e.g., 0069. Examples of various suitable additional cosmeti from about 50% to about 75%) of water. cally acceptable actives include hydroxy acids; benzoyl 0062 Yet another type of product that may be formulated peroxide: D-panthenol; UV filters such as but not limited to from a solution is an ointment. An ointment may contain a avobenzone (Parsol 1789), bisdisulizole disodium (Neo simple base of animal, vegetable, or synthetic oils or semi Heliopan AP), diethylamino hydroxybenzoyl hexyl benzo solid 10 hydrocarbons. An ointment may contain from about ate (Uvinul A Plus), ecamsule (Mexoryl SX), methyl anthra 2% to about 10% of an emollient(s) plus from about 0.1% nilate, 4-aminobenzoic acid (PABA), cinoxate, ethylhexyl to about 2% of a thickening agent(s). triazone (Uvinul T 150), homosalate, 4-methylbenzylidene 0063. The compositions useful in the present invention camphor (Parsol 5000), octyl methoxycinnamate (Octinox can also be formulated as emulsions. If the carrier is an ate), octyl salicylate (Octisalate), padimate 0 (Escalol 507), emulsion, from about 1% to about 10% (e.g., from about 2% phenylbenzimidazole sulfonic acid (Ensulizole), polysili to about 5%) of the carrier contains an emulsifier(s). Emul cone-15 (Parsol SLX), trolamine salicylate, Bemotrizinol sifiers may be nonionic, anionic or cationic. (Tinosorb S), benzophenones 1-12, dioxybenzone, drom 0064. Lotions and creams can be formulated as emul etrizole trisiloxane (Mexoryl XL), iscotrizinol (Uvasorb sions. Typically such lotions contain from 0.5% to about 5% HEB), octocrylene, oxybenzone (Eusolex 4360), Sulisoben of an emulsifier(s), while Such creams would typically Zone, bisoctrizole (Tinosorb M), titanium dioxide, zinc contain from about 1% to about 20% (e.g., from about 5% oxide; carotenoids; free radical scavengers; spin traps; ret to about 10%) of an emollient(s); from about 20% to about inoids and retinoid precursors such as 30 retinol, retinoic 80% (e.g., from 30% to about 70%) of water; and from about acid and retinyl palmitate; ceramides; polyunsaturated fatty 1% to about 10% (e.g., from about 2% to about 5%) of an acids; essential fatty acids; enzymes; enzyme inhibitors; emulsifier(s). minerals; hormones Such as estrogens: Steroids such as 0065. Single emulsion skin care preparations, such as hydrocortisone; 2-dimethylaminoethanol; copper salts such lotions and creams, of the oil-in-water type, and water-in-oil as copper chloride; peptides containing copper Such as type are well-known in the art and are useful in the subject Cu:Gly-His-Lys, coenzyme Q10; amino acids such a pro invention. Multiphase emulsion compositions, such as the line; vitamins; lactobionic acid; acetyl-coenzyme A, niacin; water-in-oil-in-water type or the oil-in-water-in-oil type, are riboflavin; thiamin: ribose; electron transporters such as also useful in the Subject invention. In general. Such single NADH and FADH2, and other botanical extracts such as oat, or multiphase emulsions contain water, emollients, and , Feverfew, Soy, mushroom extracts, and emulsifiers as essential ingredients. derivatives and mixtures thereof. 0066. The compositions of this invention can also be 0070. In certain preferred embodiments, the skin care formulated as a gel (e.g., an aqueous, alcohol, alcohol/water, compositions comprise extracts of Ampelopsis grosseden or oil gel using a suitable gelling agent(s)). Suitable gelling tata and Albizia julibrissin, and at least one additional skin agents for aqueous and/or alcoholic gels include, but are not moisturizing active agent. Examples of additional skin mois limited to, natural gums, acrylic acid and acrylate polymers, turizing agents include glycerin, propylene glycol, butylene and copolymers, and cellulose derivatives (e.g., hydroxym glycol, pentalene glycol, hexylene glycol, polyethylene gly ethyl cellulose and hydroxypropyl cellulose). Suitable gell col, polypropylene glycol, diethylene glycol, triethylene ing agents for oils (such as mineral oil) include, but are not glycol, capryl glycol, glycerol, butanediol and hexanetriol, limited to, hydrogenated butylene/ethylene?styrene copoly or mixtures thereof mer and hydrogenated ethylene/propylene?styrene copoly 0071. In certain preferred embodiments, the composi mer. Such gels typically contains between about 0.1% and tions of the present invention are skin care compositions that 5%, by weight, of Such gelling agents. comprise extracts of Ampelopsis grossedentata and Albizia US 2017/0042958 A1 Feb. 16, 2017 julibrissin and at least one additional agent for improving 0077 Compositions of the present invention may include the signs of aging. Examples of Suitable additional agents a cosmetically effective amount of one or more tropoelastin improving the signs of aging include, but are not limited to, promoters such as those described above. The compositions tropoelastin promoters, collagen promoters, retinoids, preferably include, on an active basis, from about 0.1% to hyaluronic acid, dimethylaminoethanol, N.N.N',N'-Tetrakis about 10% of the tropoelastin promoters, more preferably (2-hydroxypropyl)ethylenediamine, alpha hydrox acids, from about 0.5% to about 5% of tropoelastin promoters, and polyhydroxyacids, and combinations of two or more thereof. most preferably from about 0.5% to about 2% of the 0072 “Tropoelastin promoters,” as used herein, refers to tropoelastin promoters. a class of compounds that possess the biological activity of 0078 “Collagen promoter,” as used herein, refers to enhancing the production of tropoelastin. Tropoelastin pro compounds that possess the biological activity of enhancing moters, according to the present invention, include all natu the production of collagen. "Non-retinoid collagen promot ral or synthetic compounds that are capable of enhancing the ers' according to the present invention include all natural or production of tropoelastin in the human body. synthetic compounds that are not retinoids, or derived from 0073. Examples of suitable tropoelastin promoters retinoids, and are capable of enhancing the production of include, but are not limited to, blackberry extracts, cotinus collagen in the human body. extracts, feverfew extracts, extracts of Phyllanthus niruri 0079. Examples of suitable collagen promoters include, and bimetal complexes having copper and/or Zinc constitu but are not limited to the following: Retinoids including ents. The bimetal complex having copper and/or Zinc con retinol, retinaldehyde, and retinoic acid, extracts of feverfew stituents may be, for example, copper-zinc citrate, copper (), extracts of Centella asiatica, and Zinc oxalate, copperzinc tartarate, copper-zinc malate, extracts of Siegesbeckia Orientalis; extracts of soy; collagen copper-zinc Succinate, copper-zinc malonate, copper-zinc promoting peptides; ursolic acid; and asiaticoside. maleate, copper-zinc aspartate, copper-zinc glutamate, cop 0080 Centella asiatica, also known as Violette marronne per-zinc glutarate, copper-zinc fumarate, copper-zinc glu on Reunion Island, Gotu Kola or Indian pennywort in India, carate, copper-zinc polyacrylic acid, copper-zinc adipate, Centella repanda in North America, and Talapetraka in copper-zinc pimelate, copper-zinc Suberate, copper-zinc Madagascar, is a polymorphous herb and belongs to the aZealate, copper-zinc sebacate, copper-zinc dodecanoate, or family of Umbelliferae (Apiaceae), particularly to the combinations thereof. In a preferred embodiment, the tro Hydrocotyle subfamily. It grows wild throughout the tropics poelastin promoter is selected from blackberry extracts, and prefers moist and shady regions at an altitude of about cotinus extracts, feverfew extracts, and combinations 600 to 1200 meters above sea level. Centella asiatica has thereof. In a particularly preferred embodiment, the tro three varieties: Typica, Abyssinica, and Floridana. The herb poelastin promoter is selected from blackberry extracts, is known and used for its healing, sedative, analgesic, feverfew extracts, and combinations thereof. antidepressant, antiviral and antimicrobial properties. The 0074 By "cotinus extract, it is meant an extract of the biological activity of the herb appears to be due to the leaves of “Cotinus coggy'gria.” Such as a water extract presence of triterpene molecules in the herb. A suitable thereof, available from Bilkokoop of Sofia, Bulgaria. extract of Centella asiatica is available as TECA from Bayer 0075. By “blackberry extract,” it is meant a blend of Consumer HealthCare of Basel, Switzerland. compounds isolated from the plant of the genus Rubus, and I0081. By “extracts of Siegesbeckia Orientalis, is meant preferably Rubus fruticosus. In one embodiment, the com any of various extracts of the plant Siegesbeckia Orientalis, pounds are isolated from the flowers of the plant. In a further including Darutoside available from Sederma (Croda Inter embodiment, the compounds are isolated from dried flowers national Group of Edison, N.J.). of the plant. Such compounds may be isolated from one or I0082 Suitable collagen-promoting peptides include the more part of the plant (e.g., the whole plant, flower, seed, following matrikine peptides, (i.e., a peptide derived from , rhizome, stem, fruit and/or leaf of the plant). In a the degradation of extracellular matrix proteins—collagen, preferred embodiment, the blackberry extract is a blackberry elastin, or proteoglycan) including palmitoyl pentapeptides, leaf extract. One particularly suitable blackberry extract is in particular Pal-Lys-Thr-Thr-Lys-Ser-OH, available as produced by extracting the leaves of Rubus fruticosus with MATRIXYL from Sederma (Croda International Group of a mixture of water and ethanol compounded to an activity of Edison, N.J.); GHK copper peptide available as PROCYTE about 5% to about 10%, with a maltodextrin matrix, com from Photomedex of Montgomeryville, Pa.; Palmitoyl GHK mercially available from Symrise Inc. of Teterboro, N.J., and peptide available as Biopoeptide CL from Sederma (Croda is sold under the name “SymMatrix.’ International Group of Edison, N.J.); Biomimetic tetrapep 0076 Extracts of “Phyllanthus niruri may be harvested tides, such as those available as Chronoline Tri Peptide from and used as the whole plant, or optionally one or more parts Unipex of Québec, Canada; and Palmitoyl tri-peptide, avail of the plant (e.g., flower, seed, root, rhizome, stem, fruit able as Syn-Coll from DSM of Basel, Switzerland. and/or leaf of the plant) may be used. The Phyllanthus niruri I0083 Ursolic acid is also known as pentacyclic triterpene plant or parts thereof may be finely divided, such as by acid, Prunol, Malol, Urson, betaursolic acid and 3-Beta grinding or milling, to a powder. A Suitable milled form of Hydroxy-Urs-12-En-28-Oic Acid. It is commercially avail Phyllanthus niruri is commercially available from Raintree able for example from Sigma-Aldrich of St. Louis, Mo. Nutrition, Inc., of Carson City, Nev. Preferably, a low I0084 Asiaticoside, also known chemically as: 6-3,4- molecular weight fraction of Phyllanthus niruri is used, for dihydroxy-6-(hydroxymethyl)-5-(3,4,5-trihydroxy-6-meth instance a fraction of Phyllanthus niruri substantially free of yloxan-2-yl)oxyoxan-2-yl)oxymethyl-3,4,5-trihy molecular species having a molecular weight of greater than droxyoxan-2-yl 10,11-dihydroxy-9-(hydroxymethyl)-1,2, about 100,000 daltons. Preferably, such low molecular 6a,6b.9.12a-hexamethyl-2,3,4,5,6,6a,7,8,8a, 10,11,12,13. weight fraction is water extractable from the Phyllanthus 14b-tetradecahydro-1H-picene-4a-carboxylate) is niruri plant. commercially available for example from Bayer Santé US 2017/0042958 A1 Feb. 16, 2017

Familiale Division Serdex, 69, Boulevard Victor Hugo inhibitor or Bowman-Birk Inhibitor, B3 and deriva 93.400 SAINT-OUEN France. tives such as Niacinamide, Essential soy, Whole Soy, Soy 0085 Compositions of the present invention may include extract. In certain preferred embodiments, the melanosome a cosmetically effective amount of one or more collagen transfer inhibiting agents includes a Soy extract or niacina promoters. The compositions preferably include, on an mide. active basis, from about 0.1% to about 10% of the collagen 0090. Examples of exfoliants include, but are not limited promoters, more preferably from about 0.5% to about 5% of to, alpha-hydroxy acids such as lactic acid, glycolic acid, collagen promoters, and most preferably from about 0.5% to malic acid, tartaric acid, citric acid, or any combination of about 2% of the collagen promoters. any of the foregoing, beta-hydroxy acids such as salicylic I0086. The compositions of the present invention may acid, polyhydroxy acids such as lactobionic acid and glu further comprise at least one skin lightening active agent. conic acid, and mechanical exfoliation Such as micro Examples of Suitable skin lightening active agents include, dermabrasion. In certain preferred embodiments, the exfo but are not limited to, tyrosinase inhibitors, melanin-degra liants include glycolic acid or salicylic acid. dation agents, melanosome transfer inhibiting agents includ 0091 Examples of sunscreens include, but are not limited ing PAR-2 antagonists, exfoliants, Sunscreens, retinoids, to, avobenzone (Parsol 1789), bisdisulizole disodium (Neo antioxidants, Tranexamic acid, tranexamic acid cetyl ester Heliopan AP), diethylamino hydroxybenzoyl hexyl benzo hydrochloride, skin bleaching agents, linoleic acid, adenos ate (Uvinul A Plus), ecamsule (Mexoryl SX), methyl anthra ine monophosphate disodium salt, Chamomilla extract, nilate, 4-aminobenzoic acid (PABA), cinoxate, ethylhexyl allantoin, opaciflers, talcs and silicas, Zinc salts, and the like, triazone (Uvinul T 150), homosalate, 4-methylbenzylidene and other agents as described in Solano et al. Pigment Cell camphor (Parsol 5000), octyl methoxycinnamate (Octinox Res. 19 (550-571) and Ando et al. Int J Mol Sci 11 ate), octyl salicylate (Octisalate), padimate 0 (Escalol 507), (2566-2575). phenylbenzimidazole sulfonic acid (Ensulizole), polysili 0087. Examples of suitable tyrosinase inhibitors include cone-15 (Parsol SLX), trolamine salicylate, Bemotrizinol but, are not limited to, Vitamin C and its derivatives, Vitamin (Tinosorb S), benzophenones 1-12, dioxybenzone, drom E and its derivatives, Kojic Acid, Arbutin, resorcinols, etrizole trisiloxane (Mexoryl XL), iscotrizinol (Uvasorb hydroquinone, Flavones e.g. Licorice flavanoids, Licorice HEB), octocrylene, oxybenzone (Eusolex 4360), Sulisoben root extract, Mulberry root extract, Dioscorea Coposita root Zone, bisoctrizole (Tinosorb M), titanium dioxide, zinc extract, Saxifraga extract and the like, Ellagic acid, Salicy oxide, and the like. lates and derivatives, Glucosamine and derivatives, Fuller 0092. Examples of retinoids include, but are not limited ene, Hinokitiol, Dioic acid, Acetylglucosamine, 5.5'-dipro to, retinol (Vitamin Aalcohol), retinal (Vitamin Aaldehyde), pyl-biphenyl-2,2'-diol (Magnolignan), 4-(4- retinyl acetate, retinyl propionate, retinyl linoleate, retinoic hydroxyphenyl)-2-butanol (4-HPB), combinations of two or acid, retinyl palmitate, isotretinoin, tazarotene, beXarotene, more thereof, and the like. Examples of vitamin C deriva Adapalene, combinations of two or more thereof and the tives include, but are not limited to, ascorbic acid and salts, like. In certain preferred embodiments, the retinoid is Ascorbic Acid-2-Glucoside, Sodium ascorbyl phosphate, selected from the group consisting of retinol, retinal, retinyl magnesium ascorbyl phosphate, and natural extract enriched acetate, retinyl propionate, retinyl linoleate, and combina in vitamin C. Examples of vitamin E derivatives include, but tions of two or more thereof. In certain more preferred are not limited to, alpha-tocopherol, beta, tocopherol, embodiments, the retinoid is retinol. gamma-tocopherol, delta-tocopherol, alpha-tocotrienol, 0093 Examples of antioxidants include, but are not lim beta-tocotrienol, gamma-tocotrienol, delta-tocotrienol and ited to, water-soluble antioxidants such as Sulfhydryl com mixtures thereof, tocopherol acetate, tocopherol phosphate pounds and their derivatives (e.g., Sodium metabisulfite and and natural extracts enriched in vitamin E derivatives. N-acetyl cysteine, glutathione), lipoic acid and dihydroli Examples of resorcinol derivatives include, but are not poic acid, Stilbenoids such as resveratrol and derivatives, limited to, resorcinol, 4-substituted resorcinols like 4-alky lactoferrin, iron and copper chelators and ascorbic acid and lresorcinols such as 4-butyresorcinol (rucinol), 4-hexylre ascorbic acid derivatives (e.g., ascobyl-2-glucoside, ascor sorcinol (Synovea HR, Sytheon), phenylethyl resorcinol byl palmitate and ascorbyl polypeptide). Oil-soluble anti (Symwhite, Symrise), 1-(2,4-dihydroxyphenyl)-3-(2,4-di oxidants Suitable for use in the compositions of this inven methoxy-3-methylphenyl)-Propane (nivitol, Unigen) and tion include, but are not limited to, butylated the like and natural extracts enriched in resorcinols. hydroxytoluene, retinoids (e.g., retinol and retinyl palmi Examples of salicylates include, but are not limited to, tate), tocopherols (e.g., tocopherol acetate), tocotrienols, and 4-methoxy potassium salicylate, Salicylic acid, acetylsali ubiquinones. Natural extracts containing antioxidants Suit cylic acid, 4-methoxysalicylic acid and their salts. In certain able for use in the compositions of this invention, include, preferred embodiments, the tyrosinase inhibitors include a but not limited to, extracts containing flavonoids and iso 4-substituted resorcinol, a vitamin C derivative, or a vitamin flavonoids and their derivatives (e.g., genistein and E derivative. In more preferred embodiments, the tyrosinase diadzein), extracts containing resveratrol and the like. inhibitor comprises Phenylethyl resorcinol, 4-hexyl resorci Examples of Such natural extracts include grape seed, green nol, or ascorbyl-2-glucoside. tea, black tea, white tea, pine bark, feverfew, parthenolide 0088. Examples of suitable melanin-degradation agents free feverfew, oat extracts, blackberry extract, cotinus include, but are not limited to, peroxides and enzymes Such extract, Soy extract, pomelo extract, wheat germ extract, as peroxidases and ligninases. In certain preferred embodi Hesperedin, Grape extract, Portulaca extract, Licochalcone, ments, the melanin-inhibiting agents include a peroxide or a chalcone, 2,2'-dihydroxy chalcone, Primula extract, propo ligninase. lis, and the like. 0089. Examples of suitable melanosome transfer inhibit 0094. The additional cosmetically active agent may be ing agents including PAR-2 antagonists such as Soy trypsin present in a composition in any Suitable amount, for US 2017/0042958 A1 Feb. 16, 2017

example, in an amount of from about 0.0001% to about 20% hexyldecyl Stearate and plant, nut, and vegetable oils such as by weight of the composition, e.g., about 0.001% to about macadamia nut oil, rice bran oil, grape seed oil, palm oil, 10% such as about 0.01% to about 5%. In certain preferred prim rose oil, hydrogenates peanut oil, and avocado oil. embodiments, in an amount of 0.1% to 5% and in other 0100 What is meant by a humectant is a compound preferred embodiments from 1% to 2%. intended to increase the water content of the top layers of 0095 Compositions of the present invention may include skin (e.g., hygroscopic compounds). Examples of Suitable a cosmetically effective amount of one or more anti-inflam humectants include those found ind Chapter 35, pages matory compounds. Examples of Suitable anti-inflammatory 399-415 (Skin Feel Agents, by G. Zocchi) in Handbook of agents include Substituted resorcinols, (E)-3-(4-methylphe Cosmetic Science and Technology (edited by A. Barel, M. nylsulfonyl)-2-propenenitrile (such as “Bay 11-7082, com Paye and H. Maibach, Published in 2001 by Marcel Dekker, mercially available from Sigma-Aldrich of St. Louis, Mo.), Inc New York, N.Y.) and include, but are not limited to, tetrahydrocurcuminoids (such as Tetrahydrocurcuminoid glycerin, Sorbitol or trehalose (e.g., C.C.-trehalose, Bf3 CG, available from Sabinsa Corporation of Piscataway, trehalose, C. B-trehalose) or a salt or ester thereof (e.g., N.J.), extracts and materials derived from the following: trehalose 6-phosphate). Phellodendron amurense Cortex Extract (PCE), Non-Dena 0101 What is meant by a surfactant is a surface-active tured Soy (Glycine max), Feverfew (Tanacetum parthe agent intended to cleanse or emulsify. Examples of Suitable nium), (Zingiber officinale), Ginko (), surfactants include those found in Chapter 37, pages 431 Madecassoside (Centella asiatica extract ingredient), Coti 450 (Classification of surfactants, by L. Oldenhove de nus (Cotinus Coggy'gria), Butterbur Extract (Petasites hybri Guertechin) in Handbook of Cosmetic Science and Tech dus), Goji Berry (Lycium barbarum), Milk Thistle Extract nology (edited by A. Barel, M. Paye and H. Maibach, (Silybum marianum), Honeysuckle (Lonicera japonica), Published in 2001 by Marcel Dekker, Inc., New York, N.Y.) Basalm of Peru (Myroxylon pereirae), Sage (Salvia offici and include, but are not limited to anionic Surfactants such nalis), Cranberry Extract (Vaccinium oxycoccos), Amaranth as Sulfates, cationic Surfactants such as betaines, amphoteric Oil (Amaranthus cruentus), Pomegranate (Punica grana Surfactants such as Sodium coco glycinate, noionic Surfac tum), Yerbe Mate (Ilex paraguariensis Leaf Extract), White tants such as alkyl polyglucosides. Lily Flower Extract (Lilium candidum), Olive Leaf Extract 0102) Examples of suitable chelating agents include (Olea europaea), Phloretin (apple extract), Oat Flour those which are capable of protecting and preserving the (Aveena sativa), Lifenol (Hops: Humulus lupulus) Extract, compositions of this invention. Preferably, the chelating Bugrane P (Ononis spinosa), Licochalcone (Licorice: Gly agent is ethylenediamine tetracetic acid ("EDTA), and cyrrhiza inflate extract ingredient), Symrelief (Bisabolol and more preferably is tetrasodium EDTA, available commer Ginger extract), combinations of two or more thereof, and cially from Dow Chemical Company of Midland, Mich. the like. under the trade name, “Versene 100XL. 0096. In one embodiment, the anti-inflammatory agent is 0103 Suitable preservatives include, for example, para a resorcinol. Particularly suitable substituted resorcinols bens, quaternary ammonium species, phenoxyethanol, ben include 4-hexyl resorcinol and 4-octylresorcinol, particu Zoates, DMDM hydantoin, organic acids and are present in larly 4-hexyl resorcinol. 4-Hexyl resorcinol is commercially the composition in an amount, based upon the total weight available as “SYNOVEA HR from Sytheon of Lincoln of the composition, from about 0 to about 1 percent or from Park, N.J. 4-Octylresorcinol is commercially available from about 0.05 percent to about 0.5 percent. City Chemical LLC of West Haven, Conn. 0104 Any of a variety of conditioners which impart 0097. By “extracts of feverfew,' it is meant extracts of additional attributes. Such as gloss to the hair, are suitable for the plant "Tanacetum parthenium.” Such as may be produced use in this invention. Examples include, but are not limited according to the details set for the in US Patent Application to, Volatile silicone conditioning agent having an atmo Publication No. 2007/0196523, entitled “PARTHENOLIDE spheric pressure boiling point less than about 220° C. FREE BIOACTIVE INGREDIENTS FROM FEVERFEW Examples of suitable volatile silicones nonexclusively (TANACETUM PARTHENIUM) AND PROCESSES FOR include polydimethylsiloxane, polydimethylcyclosiloxane, THEIR PRODUCTION.” One particularly suitable fever hexamethyldisiloxane, cyclomethicone fluids Such as poly few extract is commercially available as about 20% active dimethylcyclosiloxane available commercially from Dow feverfew, from Integrated Botanical Technologies of Ossin Corning Corporation of Midland, Mich. under the trade ing, N.Y. name, “DC-345’ and mixtures thereof, and preferably 0098. A variety of other materials may also be present in include cyclomethicone fluids. Other suitable conditioners the compositions of the present invention. In certain pre include cationic polymers, including polyguarterniums, cat ferred embodiments, the composition comprises one or more ionic guar, and the like. topical ingredients selected from the group consisting of 0105. Any of a variety of commercially available pearl Surfactants, chelating agents, emollients, humectants, con escent or opacifying agents are suitable for use in the ditioners, preservatives, opaciflers, fragrances and the like. composition. Examples of Suitable pearlescent or opacifying 0099 What is meant by an emollient is a compound that agents include, but are not limited to, mono or diesters of (a) helps to maintain the soft, Smooth, and pliable appearance of fatty acids having from about 16 to about 22 carbon atoms the skin (e.g., by remaining on the skin Surface or in the and (b) either ethylene or propylene glycol; mono or diesters stratum corneum to act as a lubricant). Examples of Suitable of (a) fatty acids having from about 16 to about 22 carbon emollients include those found in Chapter 35, pages 399-415 atoms (b) a polyalkylene glycol of the formula: HO-(JO) (Skin Feel Agents, by G. Zocchi) in Handbook of Cosmetic H, wherein J is an alkylene group having from about 2 Science and Technology (edited by A. Barel, M. Paye and H. to about 3 carbon atoms; and a is 2 or 3; fatty alcohols Maibach, Published in 2001 by Marcel Dekker, Inc New containing from about 16 to about 22 carbon atoms; fatty York, N.Y.), and include, but are not limited to, petrolatum, esters of the formula: KCOOCHL, wherein K and L US 2017/0042958 A1 Feb. 16, 2017

independently contain from about 15 to about 21 carbon preferred embodiments, at least one of the first and second atoms; inorganic Solids insoluble in the shampoo composi compositions is a cleanser and the other is a lotion or cream. tion, and mixtures thereof. 0111 While the foregoing description represent exem 010.6 Any fragrance compositions suitable for use on plary embodiments of the present invention, it will be skin may be used in the composition according to the present understood that various additions, modifications and Substi invention. tutions may be made therein without departing from the 0107. In certain preferred embodiments, the present spirit and scope of the present invention. In particular, it will invention is in the form of a Substrate comprising a com be clear to those skilled in the art that the present invention position of the present invention. Any suitable Substrate may may be embodied in other specific forms, structures, be used. Examples of suitable substrates and substrate arrangements, proportions, and with other elements, mate materials are disclosed, for example, in U.S. Pat. No. rials, and components, without departing from the spirit or 7,452,547 and US2009/0241242 which are incorporated essential characteristics thereof. One skilled in the art will herein by reference in their entirety. appreciate that the invention may be used with many modi 0108. In certain preferred embodiments, the substrate is a fications of structure, arrangement, proportions, materials, wipe, glove, or a facial mask. Preferably, such embodiments and components and otherwise, used in the practice of the comprise a water-insoluble Substrate as Such is defined in the invention, which are particularly adapted to specific envi cited references above. For certain embodiments, the water ronments and operative requirements without departing insoluble Substrate may have a size and shape such that it from the principles of the present invention. The presently covers the face of a human user to facilitate placing the disclosed embodiments are therefore to be considered in all water-insoluble substrate about the face of the user as a mask respects as illustrative and not restrictive, the scope of the substrate. For example, the water-insoluble mask substrate invention being indicated by the appended claims, and not may have openings for a mouth, nose, and/or eyes of the limited to the foregoing description. It will be appreciated user. Alternatively, the water insoluble substrate may have that in the claims, the term "comprises/comprising does not no such openings. Such a configuration without openings exclude the presence of other elements or steps. In addition, may be useful for embodiments of the invention in which the singular references do not exclude a plurality. The terms “a”, water-insoluble substrate is intended to be draped over a “an”, “first”, “second, etc., do not preclude a plurality. non-facial expanse of skin or if the water-insoluble substrate is intended to be used as wipe. The water-insoluble substrate EXAMPLES may have various shapes, such as an angular shape (e.g., (O112 The following test methods were used in the rectangular) or an arcuate shape such as circular or oval. For Examples. certain embodiments, the Substrate is a glove Such as described in U.S. Published Application No 2006/0141014 Assay 1: In Vitro Filaggrin Protein Assay which is incorporated herein in its entirety. In one embodi ment of the invention, the product includes a plurality of 0113 Skin epidermal equivalents from EpiKutis system water-insoluble substrates of different shapes. (Shaanxi BioCell Biotechnology Co. Ltd., China) were used 0109) Any suitable method of applying the composition for the following tests. 3-D skin equivalent Epikutis system to the skin in need may be used. For example, the compo consists of normal, human-derived epidermal keratinocytes sition may be applied directly from a package to the skin in which have been cultured to form a highly differentiated need, by hand to the skin in need, or may be transferred from model of the human epidermis. BioCells Epikutis skin a Substrate Such as a wipe or mask, or a combination of two equivalent models, each 8 mm in diameter were used in the or more thereof. In other embodiments, the composition may following test. be applied via a dropper, tube, roller, spray, and patch or 0114. The test materials (actives) were prepared as added to a bath or otherwise to water to be applied to the below: skin, and the like. The composition may be applied in a 0115 This example illustrates the making of a culture variety of manners or forms, including, without limitation, medium with 0.001% extract. The crude extract was first as a leave-on cream, mask, and/or serum. diluted with DMSO (dimethyl sulfoxide) as a 10% solution. 0110. In further additional embodiments, the methods of Then the diluted extract was added to the cell culture media. the present invention comprise applying at least two differ Additional DMSO was added so that the concentration of ent compositions or products comprising the extracts of the extract in the cell culture media was 0.001% by weight Ampelopsis grossedentata and Albizia julibrissin to the skin. and the amount of DMSO was 0.1% in the cell culture For example, the methods may comprise applying a first media. composition comprising the extracts of Ampelopsis grossed 0116. The blank control medium in the test was culture entata and Albizia julibrissin to skin in need of improving medium containing 0.1% DMSO. skin barrier function and improving skin hydration and 0117 Upon receipt, epidermal equivalents were incu moisturization, followed by applying a second composition bated for 24 h at 37° C. in maintenance medium. After comprising the extracts of Ampelopsis grossedentata and incubation, the equivalents were treated with culture Albizia julibrissin, that is different from the first composi medium containing plant extracts or a blank control medium tion, to the skin in need of treatment. In certain preferred for another 24 h at 37° C. embodiments, the first and second composition may be 0118 3 epidermal equivalents were repeated in every test independently selected from the group consisting of lotions, group. cleansers, masks, wipes, creams, serums, gels, and the like. 0119 Sections of the skin epidermal equivalents (5um in In certain preferred embodiments, at least one of the first and thickness) were cut using a cryostat (Leica CM3050s) and second compositions is a cleanser, lotion, cream, essence, or fixed with acetone for 10 min at -20° C. Filaggrin was serum, and the other is a facial mask or wipe. In certain other detected by incubating tissue sections with mouse monoclo US 2017/0042958 A1 Feb. 16, 2017 nal antibodies directed against filaggrin (1/100, Abcam, fixed with acetone for 10 min at -20°C. Transgluminase-1 Cambridge, UK) for 2 h. Staining and visualization were was detected by incubating tissue sections with mouse performed by the streptavidin/peroxidase method using the monoclonal antibodies directed against transgluminase-1 LSAB+System-HRP with a DAKO Autostainer (Dako) in (1/800. Abcam, Cambridge, UK) for 2 h. Staining and accordance with the manufacturers instructions for use. As visualization were performed by the streptavidin/peroxidase a control of non-specific labelling, the primary antibody was method using the LSAB+System-HRP with a DAKO Auto omitted. Multiple sections of each specimen were processed stainer (Dako) in accordance with the manufacturers to ensure representative samples. To evaluate protein local instructions for use. As a control of non-specific labelling, ization, a confocal laser-scanning microscope (OLYMPUS the primary antibody was omitted. Multiple sections of each BX53) was utilized. Result of IOD (Integral Optical Den sity) was quantitatively analyzed with IPP software (Image specimen were processed to ensure representative samples. Pro Plus from Media Cybernetics, US) as the quantity of To evaluate protein localization, a confocal laser-scanning filaggrin protein. The filaggrin promotion over blank control microscope (OLYMPUS-BX53) was utilized. Result of IOD was calculated by the following formula: (Integral Optical Density) was quantitatively analyzed with IPP software as the quantity of TGM1 protein. The TGM1 The filaggrin promotion over blank control=(IOD value of specific sample-IOD value of blank promotion over blank control was calculated by the follow control)/IOD value of blank controlx100% ing formula: 0120 Statistical difference between the blank controls The TGM1 promotion over blank control=(IOD and experimental groups was determined by use of two value of specific sample-IOD value of blank tailed and equal variance hypothesis student T-test and a control), IOD value of blank controlx100% p-value less than 0.05 was considered as significant differ 0.126 Statistical difference between the blank controls CCC. and experimental groups was determined by use of two Assay 2: In Vitro Sodium L-Pyrrolidone Carboxylate (PCA) tailed and equal variance hypothesis student T-test and a Assay p-value less than 0.05 was considered as significant differ CCC. 0121 The test sample was prepared using the method given in Assay 1. The skin epidermal equivalents were treated with the culture medium containing plant extracts or Assay 4: MTT Skin Equivalent Growth State blank control medium as described in the assay 1 for further Evaluation Skin Metabolic Activity Assay PCA assay. 3 epidermal equivalents were repeated in every test group. I0127. The growth state of a skin equivalent is related to 0122) The skin epidermal equivalents were extracted the potential quality of epidermis and skin barrier to be built with 500 ul deionized water by ultrasonic wave for 30 mins. up. The skin metabolic activity is reflected in the growth Every epidermal equivalent was extracted twice. The extract state of a skin equivalent. This was evaluated using the MTT was centrifuged at 14000 rpm for 10 mins. 4 uL acetonitrile assay described as follows. and 8 ul 1M ammonium formate were added into 388 uL I0128. The test sample was prepared using the method supernatant. PCA was analyzed by RP-HPLC using a RP-18 given in Assay 1. The skin epidermal equivalents were silicagel column, mobile phase of 20 mM ammonium for treated with culture medium containing plant extracts or mate and 1% acetonitrile, pH 7.8, flow rate 1 mL/min. PCA blank control medium as described in the assay 1 for further was tested as PCA quantity per equivalence. The PCA MTT assay. promotion over blank control was calculated by the follow I0129 3 epidermal equivalents were repeated in every test ing formula: group. The PCA promotion over blank control=(the PCA quantity per equivalent of a specific sample-the 0.130. The MTT Assay is a colorimetric assay system that PCA quantity per equivalent of blank control), measures the reduction of a yellow Methylthiazolyldiphe the PCA quantity per equivalent of blank con nyl-tetrazolium bromide (MTT) into an insoluble purple trolx100%. product by the mitochondria of viable cells. 0123 Statistical difference between the blank controls I0131 The skin epidermal equivalents were treated with and experimental groups was determined by use of two MTT (3-(4.5-cimethylthiazol-2-yl)-2,5-diphenyl tetrazo tailed and equal variance hypothesis student T-test and a lium bromide, sigma) for 3 hours, followed by washing 3 p-value less than 0.05 was considered as significant differ times using Dulbecco's Phosphate Buffered Saline (DPBS). CCC. Then the equivalents were transferred to a new chamber and incubated with isopropanol over night at 4°C. After being Assay 3: In Vitro Transglutaminase-1 (TGM1) Protein dissolved, the tissues were impaled to further release lysates. Assay The lysates were measured at a wavelength of 570 nm using 0.124. The test sample was prepared using the method isopropanol as the solvent control. given in Assay 1. The skin epidermal equivalents were treated with culture medium containing plant extracts or 0.132. The skin metabolic activity was calculated by the blank control medium as described in the assay 1 for further following formula: TGM1 protein assay. 3 epidermal equivalents were repeated Skin metabolic activity rate (%)=(OD value of spe cific sample concentration-OD value of control in every test group. without DMSO)/(OD value of blank control 0.125 Sections of the skin epidermal equivalents, 5 um in group-OD value of control without DMSO)x thickness, were cut using a cryostat (Leica CM3050s) and 100. (OD: optical density). US 2017/0042958 A1 Feb. 16, 2017

0.133 And the promotion of skin metabolic activity over (Sodium Lauryl Sulfate, Sigma) and culturing for 3 h or 6 h blank control was calculated by the following formula, at 37+1° C., 5+1% CO, 95% Hm. The samples were then 0134. The promotion of skin metabolic activity over washed 15 times with DPBS. blank control=skin metabolic activity rate of specific sample 0145 The epidermal equivalents were treated with MTT (%)-skin metabolic activity rate of blank control (%). (3-(4.5-cimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bro 0135 Statistical difference between the blank controls mide, Sigma) for 3 h. This was followed by washing the and experimental groups was determined by use of two equivalents 3 times using Dulbecco's Phosphate Buffered tailed and equal variance hypothesis student T-test and a Saline (DPBS), and then transferring to a new chamber and p-value less than 0.05 was considered as significant differ incubating with isopropanol over night at 4°C. After being CCC. dissolved, the tissues were impaled to further release lysates. The lysates were measured at a wavelength of 570 nm using Assay 5: In Vitro Loricrin (LOR) Protein Assay isopropanol as the solvent control. 0136. The test sample was prepared using the method 0146 The skin metabolic activity rate reflects the pen given in Assay 1. The skin epidermal equivalents were etration of SLS and skin barrier function. The skin metabolic treated with culture medium containing plant extracts or activity was calculated by the following formula: blank control medium as described in the assay 1 for further Skin metabolic activity rate (%)=(OD value of spe LOR protein assay. cific sample concentration-OD value of control without DMSO)/(OD value of blank control 0.137 3 epidermal equivalents were repeated in every test group-OD value of control without DMSO)x group. 100. (OD: optical density). 0.138. Sections of the skin epidermal equivalents, 5 um in thickness, were cut using a cryostat (Leica CM3050s) and 0147 Statistical difference between the blank controls fixed with acetone for 10 min at -20° C. Loricrin was and experimental groups was determined by use of two detected by incubating tissue sections with mouse monoclo tailed and equal variance hypothesis student T-test and a nal antibodies directed against loricrin (1/250, Abcam, Cam p-value less than 0.05 was considered as significant differ bridge, UK) for 2 h. Staining and visualization were per CCC. formed by the streptavidin/peroxidase method using the Assay 7: Gene Expression Study LSAB+System-HRP with a DAKO Autostainer (Dako) in accordance with the manufacturers instructions for use. As 0.148. The test sample was prepared using the method a control of non-specific labelling, the primary antibody was given in Assay 1. omitted. Multiple sections of each specimen were processed 0149 Human keratinocytes were inoculated in 6-well to ensure representative samples. To evaluate protein local culture plates, maintained at 37° C., 5% CO2. When 40% ization, a confocal laser-scanning microscope (OLYMPUS confluence was achieved, the keratinocytes were incubated BX53) was utilized. Result of IOD (Integral Optical Den with culture medium containing plant extracts or blank sity) was quantitatively analyzed with IPP software as the control medium (culture medium containing 0.1% DMSO) quantity of LOR protein. The LOR promotion over blank for 24 h at 37° C., 5% CO2. After 24 h of incubation, total control was calculated by the following formula: RNA was extracted with TRIZolRreagent (Life Science) and The LOR promotion over blank control=(IOD value reversely transcripted using PrimeScript(RRT reagent Kit of specific sample-IOD value of blank control), Perfect Real Time (TakaRa). The primers in the study were IOD value of blank controlx100% from Takara (Dalian, Conn.), including LOR (Genbank 0139 Statistical difference between the blank controls number NM-00427), TGM1 (Genbank number: NM 00359. and experimental groups was determined by use of two 2), FLG (Genbank number: NM002016) and Caspase-14 tailed and equal variance hypothesis student T-test and a (Genbank number: NM 012114.2). Quantitative RT-PCR p-value less than 0.05 was considered as significant differ was performed using SYBR Green Real time PCR Master CCC. Mix (TakaRa). The reaction was performed in CFX96 Assay 6: Skin Barrier Quality Evaluation with Penetration Detection System (BIO-RAD). The gene expression result Assay of SLS was analyzed by using real-time quantitative PCR and the 0140) Skin with high quality skin barrier can effectively method 2AA. block the penetration of external harmful material into the 0150. The promotion of gene expression vs blank control skin and cause the reduction of skin metabolic activity. A was calculated using the following formula: higher skin metabolic activity mean less penetration of The promotion of gene expression vs blank control (%)=(the gene expression of specific sample harmful material and further shows skin with a higher skin gene expression of blank control), gene expres barrier function. sion of blank controlx100%. 0141. In this study we used Sodium Lauryl Sulfate (SLS) as the harmful material. 0151 Statistical difference between the blank controls 0142. The test sample was prepared using the method and experimental groups was determined by use of two given in Assay 1. The skin epidermal equivalents were tailed and equal variance hypothesis student T-test and a treated with culture medium containing plant extracts or p-value less than 0.05 was considered as significant differ blank control medium as described in the assay 1 for further CCC. skin metabolic activity assay. 0143) 3 epidermal equivalents were repeated in every test Assay 8: Collagen Generation Assay group. 0152 Collagen is the most abundant connective material 0144. After being transferred to a 6-wells plate, the within the dermis, which is a fibrous protein whose primary equivalents were topically treated with 1% Triton X-100 function is to maintain skin firmness. With age, the amount US 2017/0042958 A1 Feb. 16, 2017

of collagen in the skin decreases C. Castelo-Branco, M. ELISA assays (Invitrogen Ltd, IL1C.: Catalog KAC1191, Duran, J. González-Merlo (1992) Skin collagen changes TNFO. kit: Catalog KHC3011, IL8 kit: Catalog KHC0081, related to age and hormone replacement therapy’ Maturitas IL6 kit: Catalog KHCOO61) were used to determine cytokine (1992) October; 15(2): 113-9. Exogenous aging induced by levels. UV, smoke, pollution and lifestyle also leads to goes with (O157. The inhibition of cytokine Induced by UVB Vs collagen degradation L. Baumann (2007). Skin ageing and Blank Control equivalents which were damaged by UVB its treatment” Journal of Pathology (2007): 211: 241-251 exposure, 96 was calculated as the following formula: Jerry L. Mccullough and Kristen M. Kelly (2006) “Preven The inhibition of cytokine Induced by UVB Vs tion and Treatment of Skin Aging Ann. N.Y. Acad. Sci. Blank Control equivalence which were dam (2006) 1067: 323-331. aged by UVB exposure, %–(T24 of blank con 0153. T-75 cm flasks of human fibroblast were washed trol-T24 of specific sample)/(T24 of blank con twice in PBS, placed on ice and 2 ml of cold RIPA Lysis trol-TO of blank control)x100%. Buffer was added (Beyotime, Conn.). Cells were collected 0158 Statistical difference between the blank controls by Scraping and the Supernatant was centrifuged for 20 min and experimental groups was determined by use of two at 12000 r/min. The human fibroblast were inoculated in tailed and equal variance hypothesis student T-test and a 6-well culture plates, maintained at 37° C., 5% CO2. When p-value less than 0.05 was considered as significant differ about 50% confluence was achieved, the human fibroblast CCC. were incubated with culture medium containing plant 0159. The following examples illustrate the preparation extracts or blank control medium (culture medium contain and efficacy of Ampelopsis grossedentata extracts and Albi ing 0.1% DMSO) for 24 h at 37° C., 5% CO2. After 24 h of Zia julibrissin extracts incubation, total collagen was extracted. The protein con centration was determined using the Bicinchoninic Acid Example 1 Protein Assay (Bio-Rad Laboratories, Hercules, Calif.) as per the manufacturer's protocol. Preparation of Ampelopsis grossedentata Extract 0154 For immunoblot analysis, 40 ug protein was sub from Leaves (E1) jected to SDS-PAGE using 10% Tris-HC1 gel. The protein was transferred onto a nitrocellulose membrane and blocked 0160 Ampelopsis grossedentata plants were collected in with 5% dry milk for 2 h and then probed with primary China. Species identification was based on gross morpho antibody incubated overnight at 4° C. Primary antibodies logical characteristics Flora of China Editorial Committee. used in this study were monoclonal anti-collagen I (1:500, Flora Reipublicae Popularis Sinicae. Beijing: Science Press, Abcam, Cambridge, UK) or monoclonal anti-elastin (1:200, 1998, 48: 53. Plants were cleaned of soil and debris and Abcam, Cambridge, UK). The membrane was probed with separated into aerial parts and roots. Approximately 1000 g an appropriate primary antibody followed by a secondary of leaves were homogenized in a blender with 5000 mL of horseradish peroxidase-conjugated antibody incubated for 2 95% ethanol/water; the suspension was maintained in con h (1: 1000, Beyotime, Conn.). The protein levels were stant motion for 24 hours. The resulting Suspension was then detected according to the specification of DAB Horseradish filtered and dried under low pressure using a rotary evapo Peroxidase Color Development Kit (Beyotime, Conn.). The rator not exceeding 40° C. After filtration, the left over raw quantification of protein was calculated as IOD (Integral material was again extracted as described above. The com Optical Density) by a digital analysis of protein bands using bined dry mass from both extractions was designated the IPP software. The data are expressed as the relative density crude extract, approximately 270 g, for a yield of 27.0% of the protein normalized to 3-actin. And the collagen (E1). improvement vs blank control was calculated as following 0.161. A characteristic HPLC fingerprint of Ampelopsis formula: grossedentata extract can be obtained at 292 nm with gradient elute of A (Water+0.1% formic acid): B (Acetoni The collagen improvement vs blank control=(IOD of specific sample-IOD of blank control), IOD of trile) from 90:10 to 0:100. Dehydromyricetin was identified blank controlx100%. by HPLC-MS and quantified by HPLC-DAD. The content of dehydromyricetin in the extract is about 85.0%. O155 Statistical difference between the blank controls and experimental groups was determined by use of two Example 2 tailed and equal variance hypothesis student T-test and a p-value less than 0.05 was considered as significant differ Preparation of Albizia julibrissin Extract from CCC. Flowers (E2) Assay 9: ELISA Assay for Cytokines 0162 Albizia julibrissin plants were collected in China. Species identification was based on gross morphological 0156 Keratinocyte cells were plated at a concentration of characteristics Flora of China Editorial Committee. Flora 2x10/cm in 6-well plates. When plating efficiency Reipublicae Popularis Sinicae. Beijing: Science Press, 1998, achieved about 45% per well, the keratinocytes were incu 39: 65). Plants were cleaned of soil and debris and separated bated with culture medium containing plant extracts or blank into aerial parts and roots. Approximately 2000 g of flowers control medium (culture medium containing 0.1% DMSO) were homogenized in a blender with 12,000 mL of 95% for 24 h at 37° C., 5% CO2. After 24 h incubation, ethanol/water, the Suspension was maintained in constant Supernatant was collected served for To time-point. Mean motion for 24 hours. The resulting Suspension was then while, cells were exposed to 300 m.J/cm of UVB radiation. filtered and dried under low pressure using a rotary evapo Control cells were shaved but not exposed to UV. 24h later, rator not exceeding 40° C. After filtration, the left over raw Supernatant was collected which served as a Ta time-point. material was again extracted as described above. The com US 2017/0042958 A1 Feb. 16, 2017

bined dry mass from both extractions was designated the TABLE 2 crude extract, approximately 144 g for a yield of 7.2% (E2). 0163 A characteristic HPLC fingerprint of Albizia Results of Sodium L-pyrrollidone carboxylate (PCA) and PCA Julibrissin extract can be obtained at 348 nm with gradient promotion over blank control in an epidermal equivalent model (%) elute of A (Water+0.1% formic acid): B (Acetonitrile) from 90:10 to 0:100. Quercitrin was identified by HPLC-MS and PCA PCA Promotion Vs quantified by HPLC-DAD. The content of Quercitrin in Extract and % gTissue Blank Control Albizia Julibrissin is about 7.74%. Blank Control

Example 3 E1, O 186 -- E2, O Determination of Filaggrin Protein Promotion E1, 0.00025% 2.08 12.05% E1, 0.0005% 2.27 22.36% 0164. Extracts E1, E2 and combinations of the two at 1:1 E1, 0.001% 2.55 36.98%: (0.001% E1+0.001% E2) were tested for filaggrin protein E1, 0.0015% 2.60 40.08%* levels using the method of Assay 1 described above. The E1, 0.001 75% 2.74 47.58%: crude extract was diluted with DMSO (dimethyl sulfoxide) E1, 0.002% 2.82 51.68%: as a 10% solution. Then the diluted extract was added to the E2, 0.00025% 1.96 5.33% cell culture media. Additional DMSO was added so that the E2, 0.0005% 2.08 12.16% E2, 0.001% 2.67 43.57%* concentration of the extract in the cell culture media was E2, 0.0015% 2.56 37.68%: 0.001% by weight and the amount of DMSO was 0.1% in E2, 0.001 75% 2.56 37.94%: the cell culture media. The blank control was the 0.1% E2, 0.002% 2.74 47.29%: DMSO solvent by weight in the cell culture media. E1, 0.002% 4.19 125.62%: (0165. The results are given in Table 1 below. E2, 0.002% E1, 0.0015% 3.42 83.99%: TABLE 1. E2, 0.0015% E1, 0.001% 3.52 89.60%: Results of filaggrin protein promotion in a epidermal equivalent model E2, 0.001% Filaggrin promotion over blank control (%) E1, 0.0005% 2.88. 54.99%: Extracts Average of Three Tests of Each Extract or E2, 0.0005% (wt %) Extract Combination E1, 0.00025% 2.41 29.77% Blank Control E2, 0.00025% E1 (0.001) 6.31 E1, 0.00025% 2.93 57.93%: E2 (0.001) -2.82 E2, 0.001 75% E1 (0.002) 3.32 E1, 0.0015% 3.14 68.84%* E2 (0.002) 2.96 E1 - E2 12.90: E2, 0.0005% (0.001 + 0.001) E1, 0.001 75% 3.23 73.80%: E2, 0.00025% *= p < 0.05 vs blank control (student T-test, two-tailed and equal variance hypothesis). *= p < 0.05 vs blank control (student T-test, two-tailed and equal variance hypothesis). 0166 Based on these result, it can be concluded that application of the combination of Ampelopsis grossedentata 0169 Based on the results demonstrated in examples 3 extract and Albizia julibrissin extract has a synergistic effect and 4, it can be concluded that topical application of the to promote filaggrin protein of skin. Therefore, the combi nation of Ampelopsis grossedentata extract and Albizia combination of Ampelopsis grossedentata extract and Albi julibrissin extract would be expected to have the effect to Zia julibrissin extract has a synergistic effect to promote the improve skin hydration and reinforce the skin barrier struc generation of filaggrin protein of skin and Natural Moistur ture and therefore improve the skin's defense against exter izing Factors (NMFs) of the skin, thereby improving skin nal antigen penetration that causes skin inflammation which barrier integrity, and improving water binding capability of is involved in compromised skin problems and protect the the skin, and thus, reducing skin inflammation which is skin from internal water loss. induced by a reduction of filaggrin and improving skin hydration and relieving skin dryness. Example 4 (0170 Based on these results it can be concluded that that application of the combination of Ampelopsis grossedentata Determination of Sodium L-Pyrrolidone extract and Albizia julibrissin extract can improve PCA. And Carboxylate (PCA) Promotion the application of the combination of Ampelopsis grossed entata extract and Albizia julibrissin extract has synergistic 0167 Extracts E1, E2 and combinations of the two at 1:1, effect to promote PCA generation of skin the combination 3:1, 7:1, 1:7 weight ratios were tested for Sodium L-pyr ratio is from 12.5% to 87.5% (1:7 to 7:1). The total con rolidone carboxylate (PCA) levels using the method of centration of the combination of Ampelopsis grossedentata Assay 2 described above. Each samples was tested three extract and Albizia julibrissin extract can be very effective times. from lower to 0.0005% (W/W), to up to 30% (W/W) when 0168 The results are given in Table 2 below. considering the trans-epidermal penetration. US 2017/0042958 A1 Feb. 16, 2017

Example 5 Example 7 Skin Equivalence Growth State Evaluation Skin Determination of Loricrin (LOR) Promotion Metabolic Activity Assay 0177 Extracts E1, E2 and combinations of the two 0171 The skin metabolic activity potential for E1, E2, extracts were tested for Loricrin (LOR) levels using the and combination of E1 and E2, was determined by the MTT method of Assay 5 described above. The results are given in test given in Assay 4 above. Table 5 below. (0172. The results are given in Table 3 below. TABLE 5 TABLE 3 Results of Loricrin (LOR) promotion over blank control in an epidermal equivalent model The promotion of skin metabolic activity In an epidermal equivalent model LOR Promotion vs Blank Control Extracts (wt %) (%) The promotion of metabolic activity of epidermal Control equivalent over blank control E1 (0.001) -3.04 Treatment Dose, as W.W (%) E2 (0.001) -6.27 E1 (0.001 + E2 (0.001) 7.60% Blank Control E1, 0.002% 3.23 *= p < 0.05 vs blank control (student T-test, two-tailed and equal variance hypothesis). E2, 0.002% -6.08 E1 O.OO1% - E2 O.OO1% 8.66* 0.178 Based on these results, it can be concluded that application of the combination of ampelopsis grossedentata *= p < 0.05 vs blank control (student T-test, two tailed and equal variance hypothesis). extract and Albizia Julibrissin extract has a synergistic effect 0173 Based on these results, it can be concluded that by to promote loricrin protein of the skin. Therefore, the addition of Ampelopsis grossedentata extract to the Albizia combination of Ampelopsis grossedentata extract and Albi julibrissin extract significantly improves skin metabolic Zia julibrissin extract would be expected to have synergistic activity which has potential to create significant higher effect to improve LOR protein of aged skin. Also they would quality of skin barrier. be expected to improve skin hydration and reinforce the skin barrier structure and therefore improve the skin's defense against external antigen penetration that causes skin inflam Example 6 mation which is involved in compromised skin problems and protect the skin from internal water loss. Determination of Transglutaminase-1 (TGM1) Promotion Example 8 0174 Extracts E1, E2 and combinations of the two extracts were tested for Transglutaminase-1 (TGM1) levels Effect on Gene Expression using the method of Assay 3 described above. 0179 Extracts E1, E2 and combinations of the two (0175. The results are given in Table 4 below. extracts were tested for their effects on gene expression using the assay mentioned in Assay 7 above. The genes TABLE 4 assayed were Loricrin (LOR), Transglutaminase-1 (TGM1), Filaggrin (FLG) and Caspase 14 (CASP14). Results of Transglutaminase-1 (TGM1) promotion over blank control in an epidermal equivalent model 0180. The results are given in Table 6 below. Extracts (wt.%) TGM1 Promotion vs Blank Control TABLE 6 Control Effect on gene expression E1 (0.001) -0.45% E2 (0.001) -0.72% Gene expression E1 (0.002) 2.39% promotion vs E2 (0.002) O.16% blank control, 96 LOR TGM1 FLG CASP14 E1 (0.001 + E2 (0.001) 6.6196* E2 O.OO125% -31.40% -5.00% 18.90% -0.60% *= p < 0.05 vs blank control (student T-test, two-tailed and equal variance hypothesis). E2 O.OO125% - 18.20% 20.70%: 111.90%* 310.50%: E1 O.OO125% 0176 Based on these results, it can be concluded that E1 O.OO125% -46.70%* 4.20% 46.70% 3.00% application of the combination of Ampelopsis grossedentata *= p < 0.05 vs blank control (student T-test, two-tailed and equal variance hypothesis). extract and Albizia julibrissin extract has a synergistic effect to promote transglutaminase-1 protein of skin. Therefore, 0181 Based on these results, it can be concluded that that the combination of Ampelopsis grossedentata extract and application of the combination of Ampelopsis grossedentata Albizia julibrissin extract would be expected to have a extract and Albizia julibrissin extract can significantly synergistic effect to improve skin hydration and reinforce improve the gene expression of FLG.Caspase-14 and the skin barrier structure and therefore improve the skins TGM1. Therefore, the combination of Ampelopsis grossed defense against external antigen penetration that causes skin entata extract and Albizia julibrissin extract would be inflammation which is involved in compromised skin prob expected to have synergistic effect to improve skin hydration lems and protect the skin from internal water loss. and reinforce the skin barrier structure and therefore US 2017/0042958 A1 Feb. 16, 2017 17 improve the skin's defense against external antigen penetra- consequence prevents skin allergy and inflammation, and tion that causes skin inflammation which is involved in other oxidation related compromised skin problems. compromised skin problems and protect the skin from internal water loss. Example 10 0182 Based on these results, it can be concluded that that application of the combination of Ampelopsis grossedentata extract and Albizia julibrissin extract can improve the gene Determination of Collagen Generation expression of LOR. Therefore, the combination of ampelop sis grossedentata extract and Albizia julibrissin extract 0186 Extracts E1, E2 and combinations of the two would be expected to improve LOR protein of aged skin. extracts were tested for their effect on collagen generation Also they would be expected to improve skin integrity and using the method of Assay 8 described above. reinforce skin barrier structure and therefor to improve skin defense to external antigen penetration into skin to cause 0187. The results are given in Table 8 below. skin inflammation which is involved the compromised skin problems and protect skin from internal water loss. TABLE 8 Example 9 Determination of collagen generation in a human fibroblast model IOD of Collagen Promotion vs Determination of Skin Barrier Function by the Sample Collagen blank control, 96 Penetration Assay of SLS in an Epidermal Blank Control O.142 Equivalence Model E2 O.OOO625% 0.144 1.41% E2 O.OO125% O.215 S1.41% 0183 Skin barrier function evaluation for E1, E2, and E1 O.OO125% 0.155 8.80% combination of E1 and E2, was conducted by the penetration E1 O.OO25% O.123 - 13.73% assay of SLS shown in Assay 6 above. The skin metabolic E2 O.OOO625% - O.338 137.68%: activity was tested. The results are given in Table 7 below. E1 O.OO125% *= p < 0.05 vs blank control (student T-test, two-tailed and equal variance hypothesis). TABLE 7 Results for skin barrier function evaluation using SLS 0188 From the above example 10 it can be seen that the enetration assav in an epidermal equivalent model inventive combination of Ampelopsis grossedentata and Albizia julibrissin extracts can significantly improve the Skin Metabolic Activity generation of collagen, and therefore, have a synergistic Cell Viability (%) 3h 6h effect to protect skin from aging. E1 (0.001%) + E2 (0.001%) 56.6%: 29.66%: treated equivalents, Example 11 Damaged by 1% SLS Blank Control Equivalence, 36.88% 12.61% Damaged by 1% SLS Anti-Inflammation Assay for Inhibition of Cytokine p = < 0.05 vs blank control equivalence which damaged by 1% SLS at same time point Induced by UVB (student T-test, two-tailed and equal variance hypothesis), 0184 Based on these results, it can be concluded that that (0189 Extracts E1, E2 and combinations of the two application of the inventive combination of ampelopsis extracts were tested for their effect on anti-inflammation for grossedentata extract and albizia julibrissin extract can inhibition of cytokines induced by UVB using the method of create a robust skin barrier which can effectively block Assay 9 described above. The results are given in Table 9 harmful material penetration into skin to reduce skin meta- below. bolic activity. Therefore, the combination of ampelopsis grossedentata extract and albizia julibrissin extract would TABLE 9 be expected to have effect to increase skin integrity and Anti-inflammation Assay for Inhibition of Cytokine reinforce skin barrier structure and improve skin hydration induced by UVB in a keratinocyte model and reinforce the skin barrier structure and therefore improve the skin's defense against external antigen penetra- Cytokine Induced by UVB, tion that causes skin inflammation which is involved in Cytokine Inhibition Vs Control, 96 compromised skin problems and protect the skin from IL-1C. 50.95%: internal water loss. IL-6 85.00%: 0185. From the above examples 6, 7, 8 and 9 it can be IL-8 45.68%: seen that combinations of Ampelopsis grossedentata and TNFC 102.28%: Albizia julibrissin extracts would be expected to increase the *= p < 0.05 vs blank control with UVB exposure (student T-test, two-tailed and equal hydration levels of the skin, improve the structure of stratum variance hypothesis), corneum, reinforce skin barrier structure, and reduce the penetration of outside antigens into skin thereby preventing 0190. From the above example 11, it can be seen that the skin cell damage, and also protect skin from internal water combination of Ampelopsis grossedentata and Albizia loss. The combination of Ampelopsis grossedentata and julibrissin extracts can significantly improve the inhibition Albizia julibrissin extracts, thus, is capable of improving the of cytokines which induced by UVB exposure, and there defense to intrinsic and extrinsic stimulation, and as a fore, have a synergistic effect for anti-inflammation. US 2017/0042958 A1 Feb. 16, 2017

Example 12 TABLE 11-continued 0191) A skin care composition according to the invention was prepared using the ingredients shown in Table 10. Trade Name INCI Name % weight

TABLE 10 Glycerin Glycerin 1O.OO Trade Name INCI Name % weight BHT BHT O.10 Benzyl Alcohol Benzyl Alcohol O60 Deionized Water Water 75.14 Natrosol 250 HHR Hydroxyethylcellulose O.SO Citric Acid Citric Acid O.O6 E1 Extract of Ampeiopsis O.1 grossedentata leaves 0194 The composition shown in Table 10 was prepared E2 Extract of Albizia O.1 as follows. Water was added to a process vessel and the julibrissin flower temperature was set to 85° C. Mixing was begun and Savonol 82 Mineral Oil 3.00 Dow Corning 345 Fluid Cyclopentasiloxane 2.50 glycerin was added and mixed until dissolved. Uceomul Dow Corning CB 9111 Cyclopentasiloxane 2.00 GMS-165 and Petrolatum were added and Miglyol 812, Dimethicone Dow Corning 9041 silicone, and isopropyl palmitate. The Dow Corning AP 8087 Bis-Hydroxy/Methoxy 1.00 Amodimethicone composition was mixed at 85°C. for another 10-15 minutes. Genamin BTLF Behentrimonium 3.0 The composition was then removed from heat and cooled. At Chloride 40° C., benzyl alcohol and Extracts of Ampelopsis grossed Tego Amid S18 Stearamidopropyl 1.O Dimethylamine entata leaves and extract of Albizia julibrissin flower were Brij 721 Steareth-21 O.S added, q.S. with water and continue to mix and cool to Lanette C1898-1OO MY Stearyl Alcohol 4.5 30-35°C. The composition was then filled into packaging. Glycerin Glycerin 6.OO Benzyl Alcohol Benzyl Alcohol O.60 Example 14 0.192 The composition shown in Table 10 was prepared 0.195 Askin care composition according to the invention as follows: water was added to a process vessel. Mixing was was prepared using the ingredients shown in Table 12. begun and Hydroxyethylcellulose was added and mixed until dissolved. Heat was applied and mixing continued until temperature reached 85°C. Glycerin was then added while TABLE 12 continuing the mixing at 85°C. GENAMIN BTLF and Tego Amid S18 was added, as was Brij 721 and Lanette C18 Trade Name INCI Name % weight 98-100 MY. Savonol 82, and Dow Corning AP 8087. The Purified water Deionized Water 85.6 composition was mixed at 85°C. for another 10-15 minutes. E1 Extract of Ampeiopsis O.1 The composition was then removed from heat and continued grossedentata leaves to mix and cooled. At 40° C. Extracts of Ampelopsis E2 Extract of Albizia O.1 grossedentata leaves and extract of Albizia julibrissin flower julibrissin flower were added to the mixture with benzyl alcohol, Dow Corn HYDROLITE 5 Pentylene glycol 3.00 ing 345 Fluid and Dow Corning CB9111 and q.s. with water Glycerin Glycerin 4.OO and continue to mix and cool to 30-35°C. The composition Montanov L C14-22 Alcohols & 2.00 was then filled into packaging. C12-20 Alkyl Glucoside FINSOLVTN C12-15 Alkyl Benzoate 4.OO Example 13 ARISTOFLEXAVC Ammonium 1.20 Acryloyldimethyl 0193 Askin care composition according to the invention tauratefVP was prepared using the ingredients shown in Table 11. Copolymer TABLE 11 Trade Name NCI Name % weight 0196. The composition shown in Table 12 was prepared Deionized Water Water 72.60 as follows. Extracts of Ampelopsis grossedentata leaves and E1 Extract of Ampeiopsis O.1 extract of Albizia julibrissin flower were weighed and dis grossedentata leaves solved in HYDROLITE 5 as a pre-mix 1 at room tempera E2 Extract of Albizia O.1 julibrissin flower ture. Then Glycerin and deionized water was added to form Snow White Petrolatum Petrolatum 4.OO Phase A with 75° C. Montanov L and FINSOLV TN were Miglyol 812 Caprylic Capric 2.50 mixed to form Phase B at 75° C. Phase B was added to Phase Triglycerides Dow Corning 9041 silicone Dimethicone (and) 2.0 A very slowly under continuous mixing. Mixing was con Dimethicone tinued for 15 minutes until a uniform emulsion was formed. Crosspolymer ARISTOFLEX was added to the emulsion under continuous Crodamol IPP sopropyl Palmitate 3.00 Uceomul GMS-165 Glyceryl S.OO mixing at high speed to obtain a thick, Smooth and homog Monostearate & PEG enous formulation. The mixture was cooled down to 32° C. OO Stearate and Pre-mix 1 was added at 32° C. to create a uniform mixture. Feb. 16, 2017

Example 15 3. The method of claim 1, wherein the weight ratio in the composition of the extracts of Ampelopsis grossedentata to 0.197 Askin care composition according to the invention Albizia julibrissin is between 1:10 to 10:1. was prepared using the ingredients shown in Table 13. 4. The method claim 2, wherein the polar extract of Ampelopsis grossedentata is a leaf extract. TABLE 13 5. The method of claim 2, wherein the polar extract of Albizia julibrissin is a flower extract. Trade Name NCI Name % weight 6. The method of claim 1 wherein the composition further Purified water Water 83.95 comprises a material selected from the group consisting of E1 Extract of Ampeiopsis O.1 Surfactants, chelating agents, emollients, humectants, con grossedentata leaves ditioners, preservatives, opacifiers, fragrances, and combi E2 Extract of Albizia O.1 nations of two or more thereof. julibrissin flower Pemulen TR-1 Acrylates/C10-30 Alkyl O.40 7. The method of claim 1 wherein said composition is a Acrylate Crosspolymer skin care composition in a form selected from the group VERSENENA Disodium EDTA O.20 consisting of lotions, creams, serums, gels, Sticks, sprays, Brij 72 Steareth-2 0.75 ointments, liquid washes, soap bars, shampoos, hair condi Brij 721 Steareth-21 1...SO tioners, pastes, foams, powders, mousses, shaving creams, FINSOLVTN C12-15 Alkyl Benzoate 4.OO Dow Corning 9041 silicone Dimethicone (and) 2.00 hydrogels, film-forming products, fluid on wipes, fluid on Dimethicone Crosspolymer facial masks, and combinations of two or more thereof. Phenonip XB Phenonip XB 1.00 8. The method of claim 2, wherein the polar extract of Glycerin Glycerin 6.OO Ampelopsis grossedentata is made by extracting with etha nol. 9. The method of claim 2, wherein the polar extract of 0198 The composition shown in Table 13 was prepared Albizia julibrissin is made by extracting with ethanol. as follows. An oil phase was prepared by adding FINSOLV 10. The method of claim 2, wherein said composition TN to a clean glass beaker. Agitation was begun and the comprises from about 0.01% to about 2% of the polar extract vessel was heated to 55-60° C. When the oil phase reached of Ampelopsis grossedentata 55° C. or higher, Brij 72 and Brij 721 were added. When the 11. The method of claim 2, wherein said composition oil phase reached 55-60° C., it was held at that temperature comprises from about 0.01% to about 2% of the polar extract and mixed for 15 min (or until uniform). The temperature of Albizia julibrissin. was then held at 55-60° C. with mixing. A water phase was 12. The method of claim3, wherein the weight ratio in the prepared by adding water and Pemulen TR-1 to a clean glass composition of the extracts of Ampelopsis grossedentata to beaker. Agitation was begun and the vessel was heated to Albizia julibrissin is between 1:7 to 7:1. 55-60° C. Disodium EDTA was added. At 55-60° C., the 13. The method of claim 12, wherein the weight ratio in ingredients were mixed for 15 min or until homogeneous. the composition of the extracts of Ampelopsis grossedentata The temperature was then held at 55-60° C. with mixing. to Albizia julibrissin is between 1:5 to 5:1. The oil phase was added to the water phase with increased 14. The method of claim 13, wherein the weight ratio in agitation and then mixed at high speed for 10-20 min. At 50° the composition of the extracts of Ampelopsis grossedentata C. or lower, Dow Corning 9041 silicone was added. At 40° to Albizia julibrissin is 1:1. C. or lower, Phenonip XB was added. The phases were then 15. The method of claim 1, wherein the total amount of mixed for 10 minor until uniform. Sodium hydroxide was the combination of the Ampelopsis grossedentata extract added (target pH was 6.0). The composition was then mixed and the Albizia julibrissin extract is from about 0.0005% to for 10 min or until uniform. Extracts of Ampelopsis grossed about 30% by weight of the composition. entata leaves and extract of Albizia julibrissin flower were 16. The method of claim 1, wherein the amount of the weighed and dissolved in Glycerin and added to the mixture. Ampelopsis grossedentata extract is from about 0.001% to This was mixed until uniform. Water was then added to QS about 10% by weight of the composition and the amount of and the composition was then mixed for 10 minutes. Albizia julibrissin extract is from about 0.001% to about (0199 While the invention has been described above with 10% by weight of the composition. reference to specific embodiments thereof, it is apparent that 17. The method of claim 16, wherein the amount of the many changes, modifications, and variations can be made Ampelopsis grossedentata extract is from about 0.01% to without departing from the inventive concept disclosed about 5% by weight of the composition and the amount of herein. Accordingly, it is intended to embrace all Such Albizia julibrissin extract is from about 0.01% to about 5% changes, modifications, and variations that fall within the by weight of the composition. spirit and broad scope of the appended claims 18. The method of claim 1, wherein the amount of the What is claimed is: Ampelopsis grossedentata extract is from about 0.001% to 1. A method of reducing inflammation in skin in need of about 10% by weight of the composition and the amount of reducing skin inflammation comprising applying to skin Albizia julibrissin extract is from about 0.001% to about exhibiting inflammation a topical composition comprising 10% by weight of the composition. an extract of Ampelopsis grossedentataa, an extract of 19. The method of claim 1, wherein the amount of the Albizia julibrissin and a topical carrier. Ampelopsis grossedentata extract is from about 0.1% to 2. The method of claim 1, wherein the extracts are polar about 5% by weight of the composition and the amount of extracts prepared using polar solvents selected from the Albizia julibrissin extract is from about 0.1% to about 5% by group of methanol, ethanol, isopropyl alcohol, n-butanol, weight of the composition. propylene glycol, water, and mixtures thereof. k k k k k