A Novel Urinary Proteomics Classifier for Non-Invasive Evaluation
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Structure-Function Relationship of Human Neutrophil Collagenase
Proc. Natl. Acad. Sci. USA Vol. 90, pp. 2569-2573, April 1993 Biochemistry Structure-function relationship of human neutrophil collagenase: Identification of regions responsible for substrate specificity and general proteinase activity (matrix metalloproteinase/stromelysin/extracellular macromolecule) TOMOHIKO HIROSE*, CHRISTY PATTERSON*, TAYEBEH POURMOTABBEDt, CARLO L. MAINARDIt, AND KAREN A. HASTY*t Departments of *Anatomy and Neurobiology, and of tMedicine, University of Tennessee, Memphis, TN 38163 Communicated by Jerome Gross, December 16, 1992 (received for review September 10, 1992) ABSTRACT The family of matrix metalloproteinases is a identity and 70% chemical similarity with human neutrophil family ofclosely related enzymes that play an inportant role in collagenase (MMP-8; NC). Preliminary data from other lab- physiological and pathological processes of matrix degrada- oratories have pointed toward the COOH-terminal domain as tion. The most distinctive characteristic of interstitial collage- a major determinant in substrate specificity. Windsor et al. nases (fibroblast and neutrophil collagenases) is their ability to (16) have demonstrated that the cysteine residue immediately cleave interstitial coilagens at a single peptide bond; however, adjacent to the COOH terminus was essential for colla- the precise region of the enzyme responsible for this substrate genolytic activity of fibroblast collagenase (FC). Recently, specificity remains to be defined. To address this question, we Murphy et al. (17) have suggested that both COOH- and generated truncated mutants of neutrophil collagenase with NH2-terminal domains ofthe active enzyme contribute to the various deletions in the COOH-terminal domain and chimeric substrate specificity of collagenase and proposed a possible molecules between neutrophil collagenase and stromelysin and role of the COOH-terminal domain in substrate binding. -
Collagenase and Elastase Activities in Human and Murine Cancer Cells and Their Modulation by Dimethylformamide
University of Rhode Island DigitalCommons@URI Open Access Master's Theses 1983 COLLAGENASE AND ELASTASE ACTIVITIES IN HUMAN AND MURINE CANCER CELLS AND THEIR MODULATION BY DIMETHYLFORMAMIDE David Ray Olsen University of Rhode Island Follow this and additional works at: https://digitalcommons.uri.edu/theses Recommended Citation Olsen, David Ray, "COLLAGENASE AND ELASTASE ACTIVITIES IN HUMAN AND MURINE CANCER CELLS AND THEIR MODULATION BY DIMETHYLFORMAMIDE" (1983). Open Access Master's Theses. Paper 213. https://digitalcommons.uri.edu/theses/213 This Thesis is brought to you for free and open access by DigitalCommons@URI. It has been accepted for inclusion in Open Access Master's Theses by an authorized administrator of DigitalCommons@URI. For more information, please contact [email protected]. COLLAGENASE AND ELASTASE ACTIVITIES IN HUMAN AND MURINE CANCER CELLS AND THEIR MODULATION BY DIMETHYLFORMAMIDE BY DAVID RAY OLSEN A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE IN PHARMACOLOGY AND TOXICOLOGY UNIVERSITY OF RHODE ISLAND 1983 MASTER OF SCIENCE THESIS OF DAVID RAY OLSEN APPROVED: Thesis Committee / / Major Professor / • l / .r Dean of the Graduate School UNIVERSITY OF RHODE ISLAND 1983 ABSTRACT Olsen, David R.; M.S., University of Rhode Island, 1983. Collagenase and Elastase Activities in Human and Murine Cancer Cells and Their Modulation by Dimethyl formamide. Major Professor: Dr. Clinton O. Chichester. The transformation from carcinoma in situ to in vasive carcinoma occurs when tumor cells traverse extra cellular matracies allowing them to move into paren chymal tissues. Tumor invasion may be aided by the secretion of collagen and elastin degrading proteases from tumor and tumor-associated cells. -
Balloon Assisted Technique in Peripheral Interventions: a Use- Ful Tool
Pozzi Mucelli F, et al., J Angiol Vasc Surg 2019, 4: 022 DOI: 10.24966/AVS-7397/100022 HSOA Journal of Angiology & Vascular Surgery Case Report technique is rarely reported [2-5]. We describe some original applica- Balloon Assisted Technique in tions of BAT of our experience. Peripheral Interventions: A Cases Description Giant aneurysm of pancreatic-duodenal artery. Female, 65 yrs Useful Tool Abdominal CT exam identified a 5 cm aneurysm of pancreatic-du- Pozzi Mucelli Fabio, Pozzi Mucelli Roberta Antea* , Sacconi odenal artery probably due to occlusion of the origin of the celiac Francesca, Braini Massimiliano, Belgrano Manuel Gianvalerio trunk caused by Dunbar Syndrome. Follow-up CT demonstrated a and Cova Maria Assunta mild enlargement of the aneurysm and for this reason an endovascular Department of Radiology, Azienda Sanitaria Universitaria Integrata di treatment was proposed. Coiling of the aneurysm was rejected due to Trieste, Trieste, Italy the risk of occlusion of the parent artery feeding the gastroduodenal, hepatic and splenic artery and we considered to exclude the aneurysm with a flow-diverter stent. Procedure was done in local anesthesia Abstract using brachial approach. After Superior Mesenteric Artery (SMA) catheterization a 6F guiding catheter (Envoy Cardinal Health) was Balloon Assisted Technique (BAT) consists in the use of a balloon advanced at the origin of the pancreatic-duodenal artery, close to the catheter advanced in a parallel fashion to main catheter in the same aneurysm (Figure 1a). A .014 guidewire (V14 Boston) was advanced artery to overcome problems during endovascular interventions. We describe three different cases of BAT. The first case is an example distally to the aneurysm, but with a large “loop” inside it (Figure 1b). -
MINIREVIEW Complex Role of Matrix Metalloproteinases in Angiogen- Esis
Cell Research (1998), 8, 171-177 MINIREVIEW Complex role of matrix metalloproteinases in angiogen- esis SANG QING XIANG AMY* Biochemistry Division, Department of Chemistry, Florida State University, Tallahassee, Florida 32306-4390, USA ABSTRACT Matrix metalloproteinases (MMPs) and tissue in- hibitors of metalloproteinases (TIMPs) play a significant role in regulating angiogenesis, the process of new blood vessel formation. Interstitial collagenase (MMP-1), 72 kDa gelatinase A/type IV collagenase (MMP-2), and 92 kDa gelatinase B/type IV collagenase (MMP-9) dissolve ex- tracellular matrix (ECM) and may initiate and promote angiogenesis. TIMP-1, TIMP-2, TIMP-3, and possibly, TIMP-4 inhibit neovascularization. A new paradigm is emerging that matrilysin (MMP-7), MMP-9, and metal- loelastase (MMP-12) may block angiogenesis by convert- ing plasminogen to angiostatin, which is one of the most potent angiogenesis antagonists. MMPs and TIMPs play a complex role in regulating angiogenesis. An understanding of the biochemical and cellular pathways and mechanisms of angiogenesis will provide important information to al- low the control of angiogenesis, e.g. the stimulation of angiogenesis for coronary collateral circulation formation; while the inhibition for treating arthritis and cancer. Key word s: Collagenases, tissue inhibitors of metallo- proteinases, neovascularization, plasmino- gen angiostatin converting enzymes, ex- tracellular matrix. * Corresponding author: Professor Qing Xiang Amy Sang, Department of Chemistry, 203 Dittmer Laboratory of Chemistry Building, Florida State University, Tallahassee, Florida 32306-4390, USA Phone: (850) 644-8683 Fax: (850) 644-8281 E-mail: [email protected]. 171 MMPs in angiogenesis Significance of matrix metalloproteinases in angiogenesis Matrix metalloproteinases (MMPs) are a family of highly homologous zinc en- dopeptidases that cleave peptide bonds of the extracellular matrix (ECM) proteins, such as collagens, laminins, elastin, and fibronectin[1, 2, 3]. -
GLOSSARY of MEDICAL and ANATOMICAL TERMS
GLOSSARY of MEDICAL and ANATOMICAL TERMS Abbreviations: • A. Arabic • abb. = abbreviation • c. circa = about • F. French • adj. adjective • G. Greek • Ge. German • cf. compare • L. Latin • dim. = diminutive • OF. Old French • ( ) plural form in brackets A-band abb. of anisotropic band G. anisos = unequal + tropos = turning; meaning having not equal properties in every direction; transverse bands in living skeletal muscle which rotate the plane of polarised light, cf. I-band. Abbé, Ernst. 1840-1905. German physicist; mathematical analysis of optics as a basis for constructing better microscopes; devised oil immersion lens; Abbé condenser. absorption L. absorbere = to suck up. acervulus L. = sand, gritty; brain sand (cf. psammoma body). acetylcholine an ester of choline found in many tissue, synapses & neuromuscular junctions, where it is a neural transmitter. acetylcholinesterase enzyme at motor end-plate responsible for rapid destruction of acetylcholine, a neurotransmitter. acidophilic adj. L. acidus = sour + G. philein = to love; affinity for an acidic dye, such as eosin staining cytoplasmic proteins. acinus (-i) L. = a juicy berry, a grape; applied to small, rounded terminal secretory units of compound exocrine glands that have a small lumen (adj. acinar). acrosome G. akron = extremity + soma = body; head of spermatozoon. actin polymer protein filament found in the intracellular cytoskeleton, particularly in the thin (I-) bands of striated muscle. adenohypophysis G. ade = an acorn + hypophyses = an undergrowth; anterior lobe of hypophysis (cf. pituitary). adenoid G. " + -oeides = in form of; in the form of a gland, glandular; the pharyngeal tonsil. adipocyte L. adeps = fat (of an animal) + G. kytos = a container; cells responsible for storage and metabolism of lipids, found in white fat and brown fat. -
William B. Schwartz Division of Nephrology 2020 Annual Report
William B. Schwartz Division of Nephrology 2020 Annual Report We dedicate this year’s report to EVERY member of the Division for their tireless work advocating for and providing the best care to our patients, supporting each other, and continuing to move the field of nephrology forward. Table of Contents Message From The Chief ........................................................................................................................ 3 Division Family Tree .............................................................................................................................. 5 Division’s COVID-19 Pandemic Experience ............................................................................................. 6 A Special Thank You............................................................................................................................. 10 Diversity, Equity, and Inclusion ......................................................................................................... 11 Medical Volunteer Trip—Kathy Quinn, RN to Uganda ...................................................................... 13 2020 New Fellows and 2020-2021 Fellow Class .................................................................................. 14 Administration ................................................................................................................................... 15 Division Overview ............................................................................................................................... -
Tumor Cell-Derived Collagenase-Stimulatory Factor Increases Expression of Interstitial Collagenase, Stromelysin, and 72-Kda Gelatinase1
[CANCER RESEARCH 53. 3154-3158. July 1. 1993] Tumor Cell-derived Collagenase-stimulatory Factor Increases Expression of Interstitial Collagenase, Stromelysin, and 72-kDa Gelatinase1 Hiroaki Kataoka,2 Rosana DeCastro, Stanley Zucker, and Chitra Biswas3 Department of Anatomy and Cellular Biology. Tufts University School of Medicine, Boston, Massachusetts 021II ¡H. K., R. D., C. B.¡, and Departments of Research and Medicine, Veterans Affairs Medical Center. Northpon, New York 11768 ¡S.ZJ ABSTRACT MATERIALS AND METHODS The tumor cell-derived collagenase-stimulatory factor (TCSF) was pre Cells and Culture Conditions. The LX-1 human lung carcinoma cells viously purified from human lung carcinoma cells (S. M. Ellis, K. Na- were originally isolated from a tumor grown in the nude mouse by Mason beshima, and C. Biswas, Cancer Res., 49: 3385-3391, 1989). This protein Research Institute, Worcester, MA, and maintained in this laboratory (4). The is present on the surface of several types of human tumor cells in vitro and human fetal lung fibroblast cell line, HFL, and the colon fibroblast cell line, in vivo and stimulates production of interstitial collagenase in human CCD-18, were obtained from the American Type Culture Collection, Bethesda, fibroblasts. In this study it is shown that TCSF stimulates expression in MD; the HF line was isolated from human skin in this laboratory (4). These cell human fibroblasts of niKN Vfor Stromelysin 1 and 72-kDa gelatinase/type lines were maintained in Dulbecco's modified Eagle's medium containing 10% IV collagenase, as well as for interstitial collagenase. Measurement of fetal bovine serum and antibiotics. -
Russian Society of Angiologists and Vascular Surgeons Invites You to Take Part in the XXXVI International Conference on June 18-20, 2020 in Korston Hotel in Kazan
Dear colleagues! Organizing Committee of the Russian Society of Angiologists and Vascular Surgeons invites you to take part in the XXXVI International Conference on June 18-20, 2020 in Korston Hotel in Kazan. The conference will cover all developments in angiology, vascular and endovascular surgery, as well as phlebology. Our conference encompasses a wide range of topics, as usual. The program will include round tables, video broadcasting, interactive educational programs, trainings and workshops on various branches of vascular surgery. Objectives of the conference: • getting to know world trends in vascular surgery, • finding more about new technologies, including endovascular ones, • introducing new methods for diagnostics of vascular diseases, • exploring long-term results of open and endovascular interventions, • exploring potential complications and errors of various surgical techniques, • evaluating short-term and long-term results of reconstructions, and • wide exchange of experience in vascular procedures. Conference provider: Russian society of angiologists and vascular surgeons Conference agenda: I. Surgical treatment for brachiocephalic arteries: • emergency and scheduled carotid procedures in patients with cerebrovascular accidents and transient ischemic attacks, vertebrobasilar insufficiency of various nature, and approach to concomitant interventions. II. Surgery of aorta: surgical treatment for aneurysms and dissections of thoracic aorta (emergency and scheduled interventions), • emergency and scheduled treatment for abdominal aorta aneurysms, • inflammatory diseases of aorta and aortic branches, • aortic dysplasia and new approaches to treatment, • hybrid aortic repair. III. Long-term results and comparison of surgical methods for various locations. IV. Surgical treatment for aorto-ileac area and visceral aortic branches. Selecting the treatment option. V. Surgical treatment for lower limb arteries. VI. -
Inflammation and Extracellular Matrix Degradation Mediated by Activated Transcription Factors Nuclear Factor-Κb and Activator P
American Journal of Pathology, Vol. 166, No. 6, June 2005 Copyright © American Society for Investigative Pathology Matrix Pathobiology Inflammation and Extracellular Matrix Degradation Mediated by Activated Transcription Factors Nuclear Factor-B and Activator Protein-1 in Inflammatory Acne Lesions in Vivo Sewon Kang, Soyun Cho, Jin Ho Chung, Despite its common occurrence, the pathogenesis of Craig Hammerberg, Gary J. Fisher, and acne is not fully understood. Excessive shedding of ep- John J. Voorhees ithelial cells from the walls of follicles combined with increased amounts of sebum produced by associated From the Department of Dermatology, University of Michigan Medical School, Ann Arbor, Michigan sebaceous glands are two important factors that contrib- ute to follicular obstruction.4,5 This obstruction leads to the formation of microcomedos, which are believed to be the precursor lesions of acne.5 These microcomedos may evolve into clinically visible comedos (blackheads Acne is the most common skin disease, causing sig- and whiteheads) or inflammatory lesions (papules, pus- nificant psychosocial problems for those afflicted. tules, or cysts). Propionibacterium acnes (P. acnes) are Currently available agents for acne treatment, such as gram-positive, anaerobic diphtheroids that are part of the oral antibiotics and isotretinoin (Accutane), have lim- 6 normal skin flora. In the generation of inflammatory le- ited use. Thus, development of novel agents to treat sions, proliferation of P. acnes in the obstructed follicles is this disease is needed. However, the pathophysiology believed to be critical.6,7 This is based on in vitro data that of acne inflammation is poorly understood. Before P. acnes stimulate monocytes to produce proinflamma- new therapeutic strategies can be devised, knowledge tory mediators and chemotactic factors,7,8 and time- regarding molecular mechanisms of acne inflamma- tested clinical observations that antibiotics that inhibit P. -
Physical Journal Volume 79 October 2000 2138–2149
CORE Metadata, citation and similar papers at core.ac.uk Provided by Elsevier - Publisher Connector 2138 Biophysical Journal Volume 79 October 2000 2138–2149 pH- and Temperature-Dependence of Functional Modulation in Metalloproteinases. A Comparison between Neutrophil Collagenase and Gelatinases A and B Giovanni Francesco Fasciglione,* Stefano Marini,* Silvana D’Alessio,† Vincenzo Politi,† and Massimo Coletta* *Department of Experimental Medicine and Biochemical Sciences, University of Roma Tor Vergata, I-00133 Roma, and †PoliFarma, I-00155 Roma, Italy ABSTRACT Metalloproteases are metalloenzymes secreted in the extracellular fluid and involved in inflammatory patholo- gies or events, such as extracellular degradation. A Zn2ϩ metal, present in the active site, is involved in the catalytic mechanism, and it is generally coordinated with histidyl and/or cysteinyl residues of the protein moiety. In this study we have investigated the effect of both pH (between pH 4.8 and 9.0) and temperature (between 15°C and 37°C) on the enzymatic functional properties of the neutrophil interstitial collagenase (MMP-8), gelatinases A (MMP-2) and B (MMP-9), using the same Ϸ synthetic substrate, namely MCA-Pro-Leu-Gly Leu-DPA-Ala-Arg-NH2. A global analysis of the observed proton-linked behavior for kcat/Km, kcat, and Km indicates that in order to have a fully consistent description of the enzymatic action of these metalloproteases we have to imply at least three protonating groups, with differing features for the three enzymes investi- gated, which are involved in the modulation of substrate interaction and catalysis by the enzyme. This is the first investigation of this type on recombinant collagenases and gelatinases of human origin. -
Peptides and Peptidomimetics As Inhibitors of Enzymes Involved in Fibrillar Collagen Degradation
materials Review Peptides and Peptidomimetics as Inhibitors of Enzymes Involved in Fibrillar Collagen Degradation Patrycja Ledwo ´n 1,2 , Anna Maria Papini 3 , Paolo Rovero 2,* and Rafal Latajka 1,* 1 Department of Bioorganic Chemistry, Faculty of Chemistry, Wroclaw University of Science and Technology, 50-370 Wroclaw, Poland; [email protected] 2 Interdepartmental Research Unit of Peptide and Protein Chemistry and Biology, Department of Neurosciences, Psychology, Drug Research and Child Health-Section of Pharmaceutical Sciences and Nutraceutics, University of Florence, 50019 Sesto Fiorentino, Firenze, Italy 3 Interdepartmental Research Unit of Peptide and Protein Chemistry and Biology, Department of Chemistry “Ugo Schiff”, University of Florence, 50019 Sesto Fiorentino, Firenze, Italy; annamaria.papini@unifi.it * Correspondence: paolo.rovero@unifi.it (P.R.); [email protected] (R.L.) Abstract: Collagen fibres degradation is a complex process involving a variety of enzymes. Fibrillar collagens, namely type I, II, and III, are the most widely spread collagens in human body, e.g., they are responsible for tissue fibrillar structure and skin elasticity. Nevertheless, the hyperactivity of fibrotic process and collagen accumulation results with joints, bone, heart, lungs, kidneys or liver fibroses. Per contra, dysfunctional collagen turnover and its increased degradation leads to wound healing disruption, skin photoaging, and loss of firmness and elasticity. In this review we described the main enzymes participating in collagen degradation pathway, paying particular attention to enzymes degrading fibrillar collagen. Therefore, collagenases (MMP-1, -8, and -13), elastases, and cathepsins, together with their peptide and peptidomimetic inhibitors, are reviewed. This information, related Citation: Ledwo´n,P.; Papini, A.M.; to the design and synthesis of new inhibitors based on peptide structure, can be relevant for future Rovero, P.; Latajka, R. -
Matrix Metalloproteinase-13 Predominates Over Matrix Metalloproteinase-8 As the Functional Interstitial Collagenase in Mouse Atheromata
Matrix Metalloproteinase-13 Predominates Over Matrix Metalloproteinase-8 as the Functional Interstitial Collagenase in Mouse Atheromata The Harvard community has made this article openly available. Please share how this access benefits you. Your story matters Citation Quillard, T., H. A. Araujo, G. Franck, Y. Tesmenitsky, and P. Libby. 2014. “Matrix Metalloproteinase-13 Predominates Over Matrix Metalloproteinase-8 as the Functional Interstitial Collagenase in Mouse Atheromata.” Arteriosclerosis, Thrombosis, and Vascular Biology 34 (6) (April 10): 1179–1186. doi:10.1161/ atvbaha.114.303326. Published Version doi:10.1161/ATVBAHA.114.303326 Citable link http://nrs.harvard.edu/urn-3:HUL.InstRepos:32605691 Terms of Use This article was downloaded from Harvard University’s DASH repository, and is made available under the terms and conditions applicable to Other Posted Material, as set forth at http:// nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of- use#LAA NIH Public Access Author Manuscript Arterioscler Thromb Vasc Biol. Author manuscript; available in PMC 2015 June 01. NIH-PA Author ManuscriptPublished NIH-PA Author Manuscript in final edited NIH-PA Author Manuscript form as: Arterioscler Thromb Vasc Biol. 2014 June ; 34(6): 1179–1186. doi:10.1161/ATVBAHA.114.303326. MMP-13 predominates over MMP-8 as the functional interstitial collagenase in mouse atheromata Thibaut Quillard, Ph.D., Haniel Alves Araújo, Gregory Franck, Ph.D, Yevgenia Tesmenitsky, and Peter Libby, M.D. Division of Cardiovascular Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA Abstract Objective—Substantial evidence implicates interstitial collagenases of the MMP family in plaque rupture and fatal thrombosis.