High Pressure Processing of Sous-Vide Lobster (Homarus Americanus) Tails
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The University of Maine DigitalCommons@UMaine Electronic Theses and Dissertations Fogler Library Summer 8-21-2020 High Pressure Processing Of Sous-vide Lobster (Homarus Americanus) Tails Sami Ali Humaid University of Maine, [email protected] Follow this and additional works at: https://digitalcommons.library.umaine.edu/etd Part of the Food Processing Commons Recommended Citation Humaid, Sami Ali, "High Pressure Processing Of Sous-vide Lobster (Homarus Americanus) Tails" (2020). Electronic Theses and Dissertations. 3266. https://digitalcommons.library.umaine.edu/etd/3266 This Open-Access Thesis is brought to you for free and open access by DigitalCommons@UMaine. It has been accepted for inclusion in Electronic Theses and Dissertations by an authorized administrator of DigitalCommons@UMaine. For more information, please contact [email protected]. HIGH PRESSURE PROCESSING OF SOUS-VIDE LOBSTER (Homarus americanus) TAILS By Sami A. Humaid B.Sc. Hadhramout University, 2007 M.S. King Abdulaziz University, 2014 A DISSERTATION Submitted in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy (in Food and Nutrition Sciences) The Graduate School The University of Maine August 2020 Advisory committee: Denise I. Skonberg, Associate Professor of Food Science, Advisor Jason Bolton, Associate Extension Professor and Food Safety Specialist Bob Bayer, Professor of Animal and Veterinary Sciences Marry Ellen Camire, Professor of Food Science and Human Nutrition Jennifer Perry, Assistant Professor of Food Microbiology Balunkeswar Nayak, Associate Professor of Food Science ©2020 Sami Humaid All Rights Reserved ii HIGH PRESSURE PROCESSING OF SOUS-VIDE LOBSTER (Homarus americanus) TAILS By Sami A. Humaid Dissertation Advisor: Dr. Denise I. Skonberg An Abstract of the Dissertation Presented in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy (in Food and Nutrition Sciences) August 2020 Sous-vide and high pressure processing (HPP) are promising techniques in the development of high-quality seafood products. Lobsters are high-value seafood products that are highly susceptible to being overcooked using conventional methods, producing a tough and rubbery texture. Lobsters are usually sold either live or frozen due to their high perishability. The application of sous-vide cooking may provide evenly cooked lobster products with a succulent and juicy texture, while HPP may increase the shelf-life of lobster products without the use of additives. The objectives of this research were to: 1) evaluate the impact of three different sous-vide cooking conditions on physicochemical properties and consumer acceptability of lobster tails, 2) evaluate the effects of HPP application on physicochemical properties of vacuum-packaged raw and subsequently sous-vide cooked lobster tails, and on consumer acceptability of the sous-vide cooked lobster tails, 3) determine the effects of HPP on the refrigerated shelf-life of vacuum-packaged raw and subsequently sous-vide cooked lobster tails. In the first study, hand-shucked lobster tails were vacuum-packaged in boilable bags and sous-vide cooked to internal temperatures of 55, 60, and 65 °C for equivalent times values (208, 45, and 10 minutes, respectively) aimed to control the target foodborne pathogen, Listeria monocytogenes. Results revealed that sous-vide cooked lobster tails at all parameters were more tender than those conventionally cooked in boiling water. In addition, no significant differences were observed in lobster qualities among the sous-vide cooking parameters. In support of the physicochemical results for sous-vide cooked tails, hedonic testing confirmed that there were no significant differences in consumer acceptability response to the sous-vide cooking parameters. Therefore, the 65 °C for 10 minutes treatment was chosen for subsequent studies because it represents the most convenient cooking treatment. In the second study, hand-shucked raw lobster tails were high pressure processed at two moderate processing pressures (150 or 350 MPa) and two processing times (5 or 10 min), then half were subsequently sous-vide cooked at 65 °C to achieve a core temperature of 65 °C/10 minutes. Hardness of raw tails decreased in the 150 MPa/10 min samples, while the shear force to cut raw and sous-vide cooked samples increased in response to 350 MPa for 5 or 10 minutes. Although HPP induced significant textural changes, consumer acceptability of the HPP pretreated sous-vide cooked lobster tails was not influenced. The third study investigated the effects of 150 MPa or 350 MPa for 10 minutes on microbial, sensory, and physicochemical qualities of raw and subsequently sous-vide cooked (65 °C) lobster tails during 28 days of storage at 2 °C. Higher pressure (350 MPa) samples maintained acceptable quality throughout 28 days storage compared to the control and 150 MPa treatment, although a considerable histamine content was observed in raw lobsters which reached the hazard limit after 14 days in the 350 MPa treatment. Furthermore, HPP pretreatment did not contribute to additional shelf-life extension of the sous-vide cooked lobster tails. The results of these studies have important implications for the lobster industry and for consumers of value-added lobster products. These results suggest that HPP has the potential to increase the commercial availability of refrigerated raw lobster tails and to be applied in combination with sous-vide cooking to produce high-quality and consumer-acceptable ready- to-eat lobster products. DEDICATION I dedicate this manuscript with sincerest gratitude to my wife, Tayba Humaid. iii ACKNOWLEDGEMENTS I would like to express my deepest gratitude to my advisor Dr. Denise Skonberg for her constant guidance, assistance, motivation, enthusiasm, and patience throughout this study. Dr. Skonberg believed in me and gave me endless support. I am tremendously fortunate to have committee members, Dr. Bob Bayer, Dr. Mary Ellen Camire, Dr. Jason Bolton, Dr. Jennifer Perry, and Dr. Balu Nayak, for their invaluable assistance and suggestions on my research goals. I would also like to gratefully acknowledge the financial support of the Hadhramout Establishment for Human Development (HEHD) and the USDA for supporting the project. My thanks and appreciation also go to Dr. Brian Perkins for his assistance and training me on HPLC work, Kathy Davis-Dentici for her lab assistance in the microbiology lab and for training me on texture analyzer and colorimeter devices, Michael Murphy for training me on the pilot plant equipment, and Dr. Bill Halteman for statistics consultation. I would like also to acknowledge Maine Fair Trade Lobster for supplying lobster tails, and Avure Technologies (JBT Corporation) for the use of their HPP unit. I also deeply appreciate the efforts and collaborative attitude of all seafood lab members, Alison, Bouhee, Charlie, Dhriti, Holly, Juliana, Samuel, Sarah, and Teaka. My gratitude also extends to all of my friends who directly or indirectly helped me to complete this project. Finally, to my parents, wife, and my friends in Yemen for their love, prayers, sacrifices, patience, support, and enthusiasm during my academic journey. I could not be where I am today without all of you. iv TABLE OF CONTENTS DEDICATION…………………………………………………………………………...iii ACKNOWLEDGEMENTS…………….………………………………………………...iv LIST OF TABLES……………………………………………………………………… xii LIST OF FIGURES…………………………………………………………….…….... xiii Chapter 1. INTRODUCTION………………………………………………………….……….….1 1.1 Lobsters……………………………………………………………….…….….1 1.2. High pressure processing…………………………………….……….……….9 1.2.1. Effects of high pressure processing on muscle proteins……………11 1.2.2. Effects of high pressure processing on muscle texture…………….19 1.2.3. Effects of high pressure processing on muscle color………………20 1.2.4. Effects of high-pressure processing on microbial shelf-life……..…21 1.3. Sous-vide cooking…………………….………………….……..…….………23 1.3.1. Effects of the temperatures of sous-vide on muscle proteins…….…25 1.3.2. Effects of sous-vide temperatures on muscle texture………….……28 1.3.3. Effects of sous-vide temperatures on microbial shelf-life…...….….30 v 1.4. Research needs………………….……………………………………....…....34 1.5. Objectives.………………………………………………………………..….34 2. EFFECTS OF SOUS-VIDE COOKING ON PHYSICOCHEMICAL PROPERTIES AND CONSUMER ACCEPTABILITY OF LOBSTER (Homarus americanus) TAILS.…….………………………….…….…….…….36 2.1. Introduction …………….……………………………………………..…….36 2.2. Materials and Methods…...…………………………………………….……38 2.2.1. Experimental design….………………………………….………...39 2.2.2. Sample preparation.………………….……………………….…....42 2.2.3. Sous-vide cooking.….……………………………………….…….43 2.2.4. Boiling processing.…….……………………………….………….45 2.2.5. Weight loss……………………….……………………………….46 2.2.6. Moisture content….……………………………………………….46 2.2.7. Water holding capacity (WHC)...……………………...………….46 2.2.8. Salt soluble protein content….…….…………..………….………47 2.2.9. Color………………………...…………………………………….48 2.2.10. Texture.…………………………………….………………….…48 vi 2.2.11. Differential scanning calorimetry (DSC)…...………….….….…49 2.2.12. Sarcomere length (SL)………………….…….….…….….…….49 2.2.13. Sensory evaluation….…….………………….……………….…51 2.2.14. Statistical analysis.……….……….…….…….…...…….……....53 2.3. Results and Discussion………………………………………………….….53 2.3.1. Time-temperature profiles during sous-vide cooking...….……....53 2.3.2. Moisture content…………....…………………………....….……55 2.3.3. Weight loss…….……………………….…………….…….….…57 2.3.4. Water holding capacity (WHC).…….………….…….…….…….59 2.3.5. Salt