1 CURRICULUM VITAE the Johns Hopkins University School Of

CURRICULUM VITAE The Johns Hopkins University School of Medicine

Hiroshi Ashikaga, M.D., Ph.D. Date: September 30, 2018

DEMOGRAPHIC AND PERSONAL INFORMATION Current Appointments University 2018-present Associate Professor of Medicine, Division of Cardiology, Johns Hopkins University School of Medicine (https://www.hopkinsmedicine.org/profiles/results/directory/profile/2076821/hiroshi-ashikaga)

Hospital 2012-present Attending Physician, Cardiac Arrhythmia Service, Johns Hopkins Hospital

Other 2018-present Visiting Scholar, IHU Liryc, Electrophysiology and Heart Modeling Institute (L'institut de rythmologie et modélisation cardiaque), Université de Bordeaux, France (https://www.ihu-liryc.fr/)

Personal Data Cardiac Arrhythmia Service, Division of Cardiology, Department of Medicine Carnegie 568, 600 N. Wolfe Street Baltimore, Maryland 21287 Tel: 410-955-7534 Fax: 443-873-5019 Email: [email protected]

Education and Training Undergraduate 1992 Premedical course (no degree), University of Tokyo, College of Arts and Sciences, Tokyo, Japan

Doctoral/graduate 1996 M.D., University of Tokyo, Faculty of Medicine, Tokyo, Japan 2007 Ph.D., University of Tokyo, Graduate School of Medicine, Japan Thesis advisor: Ryozo Nagai, M.D. (Present position: President, Jichi Medical University, Japan)

Postdoctoral 1997 Intern in Medicine, University of Tokyo Hospital, Tokyo, Japan 2000 Resident in Medicine, Beth Israel Medical Center, New York, New York 2001 Chief Resident in Medicine, Beth Israel Medical Center, New York, New York 2004 Research Fellow in Bioengineering, Division of Cardiology, University of California, San Diego Primary Mentor: James W. Covell, M.D. (Present position: Professor Emeritus, University of California, San Diego) 2006 Research Fellow in Cardiovascular MRI, Laboratory of Cardiac Energetics, National Heart, Lung and Blood Institute (NHLBI), Bethesda, Maryland Primary Mentor: Elliot R. McVeigh, Ph.D. (Present position: Professor, University of California, San Diego) 2010 Clinical Fellow in Cardiovascular Disease, Johns Hopkins Hospital, Baltimore, Maryland 2012 Clinical Fellow in Clinical Cardiac Electrophysiology, Johns Hopkins Hospital, Baltimore, Maryland

Professional Experience 2000-2001 Instructor of Medicine, Albert Einstein College of Medicine, Bronx, New York 2007-2008 Attending Physician, Cardiovascular Center, HIMEDIC Imaging Center, Lake Yamanaka, Japan 2007 Lecturer, Fukuoka School of Health Sciences, Japan (part-time) 2012-2018 Assistant Professor of Medicine, Division of Cardiology, Johns Hopkins University School of Medicine, Baltimore, Maryland 2012-2017 Assistant Professor of Biomedical Engineering (secondary appointment), Department of Biomedical Engineering, Johns Hopkins University School of Medicine 1 2017-2018 Faculty, Malone Center for Engineering in Healthcare, Johns Hopkins University (https://malonecenter.jhu.edu/) 2018 Visiting Scholar, Kavli Institute of Theoretical Physics, University of California, Santa Barbara, California (https://www.kitp.ucsb.edu/) 2018-present Visiting Scholar, IHU Liryc, Electrophysiology and Heart Modeling Institute (L'institut de rythmologie et modélisation cardiaque), Université de Bordeaux, France (https://www.ihu-liryc.fr/) 2018-present Associate Professor of Medicine, Division of Cardiology, Johns Hopkins University School of Medicine, Baltimore, Maryland

PUBLICATIONS Original Research [OR] 1. Ashikaga H, Criscione JC, Omens JH, Covell JW, Ingels NB, Jr. Transmural left ventricular mechanics underlying torsional recoil during relaxation. Am J Physiol Heart Circ Physiol. 2004;286:H640-H647. 2. Ashikaga H, Omens JH, Ingels NB, Jr, Covell JW. Transmural mechanics at left ventricular epicardial pacing site. Am J Physiol Heart Circ Physiol. 2004;286:H2401-H2407. 3. Ashikaga H, Omens JH, Covell JW. Time-dependent remodeling of transmural architecture underlying abnormal ventricular geometry in chronic volume overload heart failure. Am J Physiol Heart Circ Physiol. 2004;287:H1994- H2002. 4. Harrington KB, Rodriguez F, Cheng A, Langer F, Ashikaga H, Daughters GT, Criscione JC, Ingels NB, Jr., Miller DC. Direct measurement of transmural laminar architecture in the anterolateral wall of the ovine left ventricle: new implications for wall thickening mechanics. Am J Physiol Heart Circ Physiol. 2005;288:H1324- H1330. 5. Ashikaga H, Covell JW, Omens JH. Diastolic dysfunction in volume overload hypertrophy is associated with abnormal shearing of myolaminar sheets. Am J Physiol Heart Circ Physiol. 2005;288:H2603-H2610. 6. Ashikaga H, Mickelsen SR, Ennis DB, Rodriguez I, Kellman P, Wen H, McVeigh ER. Electromechanical analysis of infarct border zone in chronic myocardial infarction. Am J Physiol Heart Circ Physiol. 2005;289:H1099-H1105. 7. Mickelsen SR, Ashikaga H, Raval A, DeSilva R, McVeigh ER, Kusumoto FA. Transvenous access to the pericardial space: an approach to epicardial lead implantation for cardiac resynchronization therapy. Pacing Clin Electrophysiol. 2005;28:1018-1024. 8. Moreau-Villéger V, Delingette H, Sermesant M, Ashikaga H, McVeigh ER, Ayache N. Building maps of local apparent conductivity of the epicardium with a 2-D electrophysiological model of the heart. IEEE Trans Biomed Eng. 2006;53:1457-1466. 9. Dickfeld T, Kato R, Zviman MM, Nazarian S, Dong J, Ashikaga H, Lardo AC, Berger RD, Calkins H, Halperin HR. Characterization of acute and subacute radiofrequencey ablation lesions with nonenhanced magnetic resonance imaging. Heart Rhythm. 2007;4:208-214. 10. Ashikaga H, Coppola BA, Hopenfeld B, Leifer ES, McVeigh ER, Omens JH. Transmural dispersion of myofiber mechanics: implications for electrical heterogeneity in vivo. J Am Coll Cardiol. 2007;49:909-916. 11. Hopenfeld B, Ashikaga H, McVeigh ER. Geodesic based registration of sensor data and anatomical surface image data. Ann Biomed Eng. 2007;35:1771-1781. 12. Ciaccio EJ, Ashikaga H, Kaba RA, Cervantes D, Hopenfeld B, Wit AL, Peters NS, McVeigh E, Garan H, Coromilas J. Model of reentrant ventricular tachycardia based on infarct border zone geometry predicts reentrant circuit features as determined by activation mapping. Heart Rhythm. 2007;4:1034-1045. 13. Ashikaga H, Sasano T, Dong J, Zviman MM, Evers R, Hopenfeld B, Castro V, Helm RH, Dickfeld T, Nazarian S, Donahue JK, Berger RD, Calkins H, Abraham MR, Marbán E, Lardo AC, McVeigh ER, Halperin HR. Magnetic resonance-based anatomical analysis of scar-related ventricular tachycardia: implications for catheter ablation. Circ Res. 2007;101:939-947. 14. Jöbsis PD, Ashikaga H, Wen H, Rothstein EC, Horvath KA, McVeigh ER, Balaban RS. Visceral pericardium: macromolecular structure and contribution to passive mechanical properties of the left ventricle. Am J Physiol Heart Circ Physiol. 2007;293:H3379-H3387. 15. Ashikaga H, Coppola BA, Yamazaki KG, Villarreal FJ, Omens JH, Covell JW. Changes in regional myocardial volume during the cardiac cycle: implications for transmural blood flow and cardiac structure. Am J Physiol Heart Circ Physiol. 2008;295:H610-H618.

2 16. Sasano T, Abraham MR, Chang KC, Ashikaga H, Mills KJ, Holt DP, Hilton J, Nekolla SG, Dong J, Lardo AC, Helperin H, Dannals RF, Marbán E, Bengel FM. Abnormal sympathetic innervation of viable myocardium and the substrate of ventricular tachycardia after myocardial infarction. J Am Coll Cardiol. 2008;51:2266-2275. 17. Nazarian S, Kolandaivelu A, Zviman MM, Meininger GR, Kato R, Susil RC, Roguin R, Dickfeld TL, Ashikaga H, Calkins H, Berger RD, Blumke DA, Lardo AC, Halperin HR. Feasibility of real-time magnetic resonance imaging for catheter guidance in electrophysiology studies. Circulation. 2008;118:223-229. 18. Ashikaga H, van der Spoel TIG, Coppola BA, Omens JH. Transmural myocardial mechanics during isovolumic contraction. JACC Cardiovasc Imaging. 2009;2:202-211. 19. Hopenfeld B, Ashikaga H. Origin of electrocardiographic U wave: Effects of M cells and dynamic gap junction coupling. Ann Biomed Eng. 2010;38:1060-1070. 20. Ashikaga H, Leclercq C, Wang J, Kass DA, McVeigh ER. Hemodynamic improvement in cardiac resynchronization does not require improvement in left ventricular rotation mechanics: three-dimensional tagged MRI analysis. Circ Cardiovasc Img. 2010;3:456-463. 21. Estner HL, Zviman MM, Herzka D, Miller F, Castro V, Nazarian S, Ashikaga H, Dori Y, Berger RD, Calkins H, Lardo AC, Halperin HR. The critical isthmus sites of ischemic ventricular tachycardia are in zones of tissue heterogeneity, visualized by magnetic resonance imaging. Heart Rhythm. 2011;8:1942-1949. 22. Ashikaga H, Omens JH. In vivo validation of longitudinal-circumferential area change ratio to estimate myofiber shortening in the heart. IEEE Trans Biomed Eng. 2012;59:1391-1397. 23. Ashikaga H, Arevalo H, Vadakkumpadan F, Blake RC, Bayer JD, Nazarian S, Zviman MM, Tandri H, Berger RD, Calkins H, Herzka DA, Trayanova NA, Halperin HR. Feasibility of image-based simulation to estimate ablation target in human ventricular arrhythmia. Heart Rhythm. 2013;10:1109-1116. 24. Yong Ji S, Dewire J, Barcelon B, Catanzaro J, Philips B, Nazarian S, Cheng A, Spragg DD, Tandri H, Bansal S, Ashikaga H, Rickard J, Kolandaivelu A, Sinha S, Marine JE, Calkins H, Berger RD. Phrenic nerve injury: an underrecognized and potentially preventable complication of pulmonary vein isolation using a wide area circumferential ablation approach. J Cardiovasc Electrophysiol. 2013;24:1086-1091. 25. Dewire J, Agarwal S, Khurram IK, Marine JE, Berger R, Spragg D, Ashikaga H, Rickard J, Nazarian S, Calkins H. Safety and efficacy of atrial fibrillation ablation in young patients. J Atr Fibrillation. 2013; 6: 915. 26. Khurram IM, Dewire J, Mager M, Maqbool F, Zimmerman SL, Zipunnikov V, Beinart R, Marine J, Spragg DD, Berger RD, Ashikaga H, Nazarian S, Calkins H. Relationship between left atrial appendage morphology and stroke in patients with atrial fibrillation. Heart Rhythm.2013; 10: 1843-1849. 27. Ciaccio EJ, Ashikaga H, Coromilas J, Hopenfeld B, Cervantes D, Wit AL, Peters NS, McVeigh ER, Garan H. Model of bipolar electrogram fractionation and conduction block associated with activation wavefront direction at infarct border zone lateral isthmus boundaries. Circ Arrhythm Electrophysiol. 2014;7:152-163. 28. Ashikaga H, Cammin J, Tang Q, Knudsen K, Inoue Y, Fishman EK, Taguchi K. Quantitative assessment of atrial regional function using motion estimation CT. J Comput Assist Tomogr. 2014;38:773-778. 29. Dewire J, Khurram IM, Pashakhanloo F, Spragg D, Marine JE, Berger RD, Ashikaga H, Rickard J, Zimmerman SL, Zipunnikov V, Calkins H, Nazarian S. The association of pre-existing left atrial fibrosis with clinical variables in patients referred for catheter ablation of atrial fibrillation. Clin Med Insights Cardiol 2014;8:25-30. 30. Ashikaga H, Estner HL, Herzka DA, McVeigh ER, Halperin HR. Quantitative assessment of single-image super-resolution in myocardial scar imaging. IEEE J Trans Eng Health Med. 2014;2:1-12. 31. Fukumoto K, Habibi M, Gucuk Ipek E, Khurram IM, Zimmerman SL, Zipunnikov V, Spragg DD, Ashikaga H, Rickard J, Marine JE, Berger RD, Calkins H, Nazarian S. Comparison of pre-existing versus ablation-induced late gadolinium enhancement on left atrial magnetic resonance imaging. Heart Rhythm. 2015;12:668-672. 32. Ling Z, McManigle J, Zipunnikov V, Pashakhanloo F, Khurram IM, Zimmerman SL, Philips B, Marine JE, Spragg DD, Ashikaga H, Calkins H, Nazarian S. The association of left atrial low-voltage regions on electroanatomic mapping with low attenuation regions on cardiac computed tomography perfusion imaging in patients with atrial fibrillation. Heart Rhythm. 2015;12:857-864. 33. Habibi M, Lima JA, Khurram IM, Zimmerman SL, Zipunnikov V, Fukumoto K, Spragg D, Ashikaga H, Rickard J, Marine JE, Calkins H, Nazarian S. Association of left atrial function and left atrial enhancement in patients with atrial fibrillation: cardiac magnetic resonance study. Circ Cardiovasc Imaging. 2015;8:e002769. 34. Ashikaga H, Aguilar-Rodríguez J, Gorsky S, Lusczek E, Marquitti FM, Thompson B, Wu D, Garland J. Modeling the heart as a communication system. J R Soc Interface. 2015;12:20141201. 35. Inoue YY, Al-Issa A, Habibi M, Fukumoto K, Khurram I, Venkatesh BA, Zimmerman S, Nazarian S, Calkins H, Lima JA, Ashikaga H. Quantitative tissue tracking CMR of left atrial deformation and the risk of stroke in patients with atrial fibrillation. J Am Heart Assoc. 2015;4:e001844. 3 36. Ciaccio EJ, Coromilas J, Ashikaga H, Cervantes DO, Wit AL, Peters NS, McVeigh ER, Garan H. Model of unidirectional block formation leading to reentrant ventricular tachycardia in the infarct border zone of postinfarction canine hearts. Comput Biol Med. 2015;62:254-263. 37. Khurram IM, Catanzaro JN, Zimmerman S, Zipunnikov V, Berger RD, Cheng A, Sinha S, Dewire J, Marine J, Spragg D, Ashikaga H, Halperin H, Calkins H, Nazarian S. MRI Evaluation of radiofrequency, cryothermal, and laser left atrial lesion formation in patients with atrial fibrillation. Pacing Clin Electrophysiol. 2015;38:1317-1324. 38. Deng D, Arevalo H, Pashakhanloo F, Prakosa A, Ashikaga H, McVeigh E, Halperin H, Trayanova N. Accuracy of prediction of infarct-related arrhythmic circuits from 2 image-based models reconstructed from low and high resolution MRI. Front Physiol. 2015;6:282. 39. Al-Issa A, Inoue Y, Cammin J, Tang Q, Nazarian S, Calkins H, Fishman EK, Taguchi K, Ashikaga H. Regional function analysis of left atrial appendage using motion estimation CT and risk of stroke in patients with atrial fibrillation. Eur Heart J Cardiovasc Imaging. 2016;17:788-796. 40. Chrispin J, Ipek EG, Zahid S, Prakosa A, Habibi M, Spragg DD, Marine JE, Ashikaga H, Rickard J, Trayanova NA, Zimmerman SL, Zipunnikov V, Berger RD, Calkins H, Nazarian S. Lack of regional association between atrial late gadolinium enhancement on cardiac magnetic resonance and atrial fibrillation rotors. Heart Rhythm. 2016;13:654-660. 41. Habibi M, Lima JA, Ipek EG, Zimmerman SL, Zipunnikov V, Spragg D, Ashikaga H, Rickard J, Marine JE, Berger RD, Calkins H, Nazarian S. The association of baseline left atrial structure and function measured with cardiac magnetic resonance and pulmonary vein isolation outcome in patients with drug refractory atrial fibrillation. Heart Rhythm. 2016;13:1037-1044. 42. Khurram IM, Habibi M, Gucuk Ipek E, Chrispin J, Yang E, Fukumoto K, Dewire J, Spragg DD, Marine JE, Berger RD, Ashikaga H, Rickard J, Zhang Y, Zipunnikov V, Zimmerman SL, Calkins H, Nazarian S. Left atrial LGE and arrhythmia recurrence following pulmonary vein isolation for paroxysmal and persistent AF. JACC Cardiovasc Imaging. 2016;9:142-148. 43. Fukumoto K, Habibi M, Ipek EG, Zahid S, Khurram IM, Zimmerman SL, Zipunnikov V, Spragg D, Ashikaga H, Trayanova N, Tomaselli GF, Rickard J, Marine JE, Berger RD, Calkins H, Nazarian S. Association of left atrial local conduction velocity with late gadolinium enhancement on cardiac magnetic resonance in patients with atrial fibrillation. Circ Arrhythm Electrophysiol. 2016;9:e002897 44. Otani T, Al-Issa A, Pourmorteza A, McVeigh ER, Wada S, Ashikaga H. A computational framework for personalized blood flow analysis in human left atrium. Ann Biomed Eng. 2016; 44: 3284-3294. 45. Pashakhanloo F, Herzka D, Ashikaga H, Mori S, Gai N, Bluemke D, Trayanova N, McVeigh E. Myofiber architecture of the human atria as revealed by sub-millimeter diffusion tensor imaging. Circ Arrhythm Electrophysiol 2016;9:e004133. 46. Khurram IM, Maqbool F, Berger RD, Marine JE, Spragg DD, Ashikaga H, Zipunnikov V, Kass DA, Calkins H, Nazarian S, Zimmerman SL. Association between left atrial stiffness index and atrial fibrillation recurrence in patients undergoing left atrial ablation. Circ Arrhythm Electrophysiol 2016;9:e003163. 47. Zghaib T, Ipek EG, Zahid S, Balouch MA, Misra S, Ashikaga H, Berger RD, Marine JE, Spragg DD, Zimmerman SL, Zipunnikov V, Trayanova N, Calkins H, Nazarian S. Association of left atrial epicardial adipose tissue with electrogram bipolar voltage and fractionation: Electrophysiologic substrates for atrial fibrillation. Heart Rhythm. 2016;13:2333-2339. 48. Ashikaga H, James RG. Hidden structures of information transport underlying spiral wave dynamics. Chaos. 2017;27:013106. 49. Huang D, Marine JE, Li JB, Zghaib T, Ipek EG, Sinha S, Spragg DD, Ashikaga H, Berger RD, Calkins H, Nazarian S. Association of rate-dependent conduction block between eccentric coronary sinus to left atrial connections with inducible atrial fibrillation and flutter. Circ Arrhythm Electrophysiol. 2017;10:e004637. 50. Chrispin J, Ipek EG, Habibi M, Yang E, Spragg D, Marine JE, Ashikaga H, Rickard J, Berger RD, Zimmerman SL, Calkins H, Nazarian S. Clinical predictors of cardiac magnetic resonance late gadolinium enhancement in patients with atrial fibrillation. Europace. 2017;19:371-377. 51. Inoue YY, Ambale-Venkatesh B, Mewton N, Volpe GJ, Ohyama Y, Sharma RK, Wu CO, Liu CY, Bluemke DA, Soliman EZ, Lima JAC, Ashikaga H. Electrocardiographic impact of myocardial diffuse fibrosis and scar: MESA (Multi-Ethnic Study of Atherosclerosis). Radiology. 2017;282:690-698. 52. Yang E, Ipek EG, Balouch M, Mints Y, Chrispin J, Marine JE, Berger RD, Ashikaga H, Rickard J, Calkins H, Nazarian S, Spragg DD. Factors impacting complication rates for catheter ablation of atrial fibrillation from 2003 to 2015. Europace 2017;9:241-249.

4 53. Balouch M, Ipek EG, Chrispin J, Bajwa RJ, Zghaib T, Berger RD, Ashikaga H, Calkins H, Nazarian S, Marine JE, Spragg DD. Trends in transesophageal echocardiography use, findings, and clinical outcomes in the era of minimally interrupted anticoagulation for atrial fibrillation ablation. JACC Clin Electrophysiol. 2017;3:329-336. 54. Balouch M, Gucuk Ipek E, Chrispin J, Bajwa RJ, Zghaib T, Berger RD, Ashikaga H, Nazarian S, Marine JE, Calkins H, Spragg DD. Impact of rotor temperospatial stability on acute and one-year atrial fibrillation ablation outcomes. Clin Cardiol. 2017;40:383-389. 55. Tao S, Ashikaga H, Ciuffo LA, Yoneyama K, Lima JAC, Frank TF, Weiss RG, Tomaselli GF, Wu KC. Impaired left atrial function predicts inappropriate shocks in primary prevention implantable cardioverter-defibrillator candidates. J Cardiovasc Electrophysiol. 2017;28:796-805. [PMCID: PMC5528170]. I am Corresponding Author of this paper. I conceived the idea, performed preliminary data analysis, hired and supervised a fellow to complete the data and statistical analysis, managed the project, critically revised the manuscript that the fellow wrote, satisfactorily addressed the reviewers’ concerns for publication. 56. Fung GSK, Ciuffo L, Ashikaga H, Taguchi K. Motion estimation for cardiac functional analysis using two low- dose x-ray computed tomography scans. Med Phys 2017;44:4677-4686. 57. Tao S, Way S, Garland J, Chrispin J, Ciuffo, LA, Balouch MA, Nazarian S, Spragg DD, Marine J, Berger RD, Calkins H, Ashikaga H. Ablation as targeted perturbation to rewire communication networks of persistent atrial fibrillation. PLoS One. 2017;12:e0179459. 58. Tao S, Ciuffo LA, Lima, JAC, Wu K, Ashikaga H. Quantifying left atrial structure and function using single- plane tissue-tracking cardiac magnetic resonance. Magn Reson Imaging. 2017;42:130-138. 59. Inoue YY, Soliman EZ, Yoneyama K, Ambale-Venkatesh B, Wu CO, Sparapani R, Bluemke DA, Lima JAC, Ashikaga H. Electrocardiographic strain pattern is associated with left ventricular concentric remodeling, scar, and mortality over 10 years: the Multi-Ethnic Study of Atherosclerosis (MESA). J Am Heart Assoc. 2017;6:e006624. 60. Kidoh M, Utsunomiya D, Funama Y, Ashikaga H, Nakaura T, Oda S, Yuki H, Hirata K, Iyama Y, Nagayama Y, Fukui T, Yamashita Y, Taguchi K. Vectors through a cross-sectional image (VCI): A visualization method for four-dimensional motion analysis for cardiac computed tomography. J Cardiovasc Comput Tomogr. 2017;11:468- 473. 61. Huang D, Li JB, Zghaib T, Gucuk Ipek E, Balouch M, Spragg DD, Ashikaga H, Tandri H, Sinha SK, Marine JE, Berger RD, Calkins H, Nazarian S. The extent of left atrial low-voltage areas included in pulmonary vein isolation is associated with freedom from recurrent atrial arrhythmia. Can J Cardiol. 2018;34:73-7. 62. Zghaib T, Ipek EG, Hansford R, Ashikaga H, Berger R, Marine JE, Spragg DD, Tandri H, Zimmerman S, Halperin H, Brancato S, Calkins H, Henrikson C, Nazarian S. Standard ablation versus MRI-guided ablation in the treatment of ventricular tachycardia. Circ Arrhythm Electrophysiol. 2018;11:e005973. 63. Zghaib T, Keramati A, Chrispin J, Huang D, Balouch MA, Ciuffo L, Berger RD, Marine JE, Ashikaga H, Calkins H, Nazarian S, Spragg DD. Multimodal examination of atrial fibrillation substrate: correlation of left atrial bipolar voltage using multi-electrode fast automated mapping, point-by-point mapping, and magnetic resonance image intensity ratio. JACC Clin Electrophysiol. 2018;4:59-68. 64. Aronis KN, Ashikaga H. Impact of number of co-existing rotors and inter-electrode distance on accuracy of rotor localization. J Electrocardiol. 2018;51:82-91. 65. Ashikaga H, Asgari-Targhi A. Locating order-disorder phase transition in a cardiac system. Sci Rep. 2018;8:1967. 66. Sivasambu B, Balouch MA, Zghaib T, Bajwa RJ, Chrispin J, Berger RD, Ashikaga H, Nazarian S, Marine JE, Calkins H, Spragg DD. Increased rates of atrial fibrillation recurrence following pulmonary vein isolation in overweight and obese patients. J Cardiovasc Electrophysiol. 2018;29:239-245. 67. Ciuffo LA, Inoue YY, Tao S, Ipek EG, Lima JAC, Nazarian S, Spragg DD, Marine J, Berger RD, Calkins H, Ashikaga H. Mechanical dyssynchrony of the left atrium during sinus rhythm is associated with stroke in patients with history of atrial fibrillation. Eur Heart J Cardiovasc Imaging. 2018;19:433-441. 68. Chrispin J, Misra S, Marine JE, Rickard J, Barth A, Kolandaivelu A, Ashikaga H, Tandri H, Spragg DD, Crosson J, Berger RD, Tomaselli G, Calkins H, Sinha SK. Current management and clinical outcomes for catheter ablation of atrioventricular nodal re-entrant tachycardia. Europace. 2018; 20:e51-e59. 69. Ashikaga H, Prieto-Castrillo F, Kawakatsu M, Dehghani N. Causal scale of rotors in a cardiac system. Front Phys. 2018;6:30. 70. Aronis AN, Berger RD, Calkins H, Chrispin J, Marine JE, Spragg DD, Tao S, Tandri T, Ashikaga H. Is human atrial fibrillation stochastic or deterministic? Insights from missing ordinal patterns and causal entropy-complexity plane analysis. Chaos. 2018;28:063130.

5 71. Inoue YY, Ipek EG, Khurram IM, Ciuffo L, Chrispin J, Zimmerman SL, Marine JE, Rickard J, MD; Spragg DD, Nazarian S, Kusano K, Lima JA, Berger RD, Calkins H, Ashikaga H. Relation of electrocardiographic left atrial abnormalities to risk of stroke in patients with atrial fibrillation. Am J Cardiol. 2018;122:242-247. 72. Ashikaga H, James RG. Inter-scale information flow as a surrogate for downward causation that maintains spiral waves. Chaos. 2018;28:075306. 73. Boyle PM, Hakim JB, Zahid S, Franceschi WH, Murphy MJ, Prakosa A, Aronis KN, Zghaib T, Balouch M, Ipek EG, Chrispin J, Berger RD, Ashikaga H, Marine JE, Calkins H, Nazarian S, Spragg DD, Trayanova NA. The fibrotic substrate in persistent atrial fibrillation patients: Comparison between predictions from computational modeling and measurements from focal impulse and rotor mapping. Front Physiol. 2018;9:1151 74. Ciuffo LA, Inoue YY, Tao S, Ipek EG, Zghaib T, Balouch M, Lima JAC, Nazarian S, Spragg DD, Marine JE, Berger RD, Calkins H, Ashikaga H. Intra-atrial dyssynchrony during sinus rhythm predicts recurrence after the first catheter ablation of atrial fibrillation. JACC Cardiovasc Imaging. In Press. 75. Prakosa A, Arevalo AJ, Deng D, Boyle PM, Nikolov PP, Ashikaga H, Blauer JJE, Ghafoori E, Park CJ, Blake RC, Han FT, MacLeod RS, Halperin HR, Callans DJ, Ranjan R, Chrispin J, Nazarian S, Trayanova NA. Personalized virtual-heart technology for guiding the ablation of infarct-related ventricular tachycardia. Nat Biomed Eng. In Press. 76. Wu KC, Zhang L, Haberlen SA, Ashikaga H, Brown TT, Budoff MJ, D’Souza G, Kingsley LA, Palella FJ, Margolick JB, Martinez-Maza O, Soliman EZ, Post WS. Predictors of electrocardiographic QT interval prolongation in men with human immunodeficiency virus. Heart. In Press.

Review Articles [RA] 1. Kawada S, Suzuki Y, Hinohara S, Koide S, Ono Y, Ashikaga H. Cancer screening with PET: advantages and limitations. Rinsho Byori. 2007;55:656-667. (Japanese) 2. Koide S, Ashikaga H, Suzuki Y, Kawada S. Screening of coronary artery disease using myocardial perfusion positron emission tomography in asymptomatic population. Vita – Medical Science Review. 2007;24:369-376. (Japanese) 3. Ashikaga H. Management of ventricular tachycardia. Resident Note. 2011;13:57-67. (Japanese) 4. Ashikaga H. Management of arrhythmia after acute coronary syndrome. Intensivist. 2013;5:101-110. (Japanese)

Case Reports [CR] 1. Nauffal V, Wu KC, Ashikaga H, Kirk GD, Post WS. HIV and ventricular arrhythmia susceptibility: insights from cardiac patch monitoring. J Clin Case Rep. 2017;7:11

Book Chapters, Monographs [BC] 1. Ashikaga H, Ben-Yehuda O, Chien KR. Biotechnology and cardiovascular medicine: Recombinant protein therapy. In Molecular Basis of Cardiovascular Disease, 2/e, – A Companion to Braunwald’s Heart Disease, Edited by Chien KR. Philadelphia, W.B. Saunders Company. 2004;Chapter 1:p 1-15. 2. Ashikaga H, Ben-Yehuda O, Chien KR. Coronary restenosis. In Molecular Basis of Cardiovascular Disease, 2/e, – A Companion to Braunwald’s Heart Disease, Edited by Chien KR. Philadelphia, W.B. Saunders Company. 2004;Chapter 25:p 455-469. 3. Ashikaga H, Chien KR. Blood coagulation and atherothrombosis. In Molecular Basis of Cardiovascular Disease, 2/e, – A Companion to Braunwald’s Heart Disease, Edited by Chien KR. Philadelphia, W.B. Saunders Company. 2004;Chapter 28:p 498-518. 4. Ashikaga H, Villarreal FJ. Prospects for gene therapy for the fibrosed heart: Targeting regulators of extracellular matrix turnover. In Interstitial Fibrosis In Heart Failure, Edited by Villarreal FJ. New York, Springer Science+Business Media, Inc. 2005;Chapter 17:p 343-354. 5. Ashikaga H, McVeigh ER. Imaging of myocardial mechanics. In Novel Techniques for Imaging the Heart: Cardiac MR and CT, Edited by Di Carli MF and Kwong RY. American Heart Association. Oxford, Blackwell Publishing. 2008;Chapter 21:p 328-335. 6. Ashikaga H. Myofiber orientation and left ventricular mechanics. In Diastology, Edited by Ohki T and Takenaka K. Tokyo, Japan. Bunkodo Publishing. 2010;Chapter 3:p 15-22. (Japanese) 7. Ashikaga H, Kolandaivelu A, Tandri H, Nazarian S, Halperin HR. CT and MR Imaging for electrophysiology. In Cardiac Electrophysiology: From Cell to Bedside, 6/e, Edited by Zipes DP and Jalife J. 2014;Chapter 61:p 595- 603.

6 8. Ashikaga H. Complication of Acute Coronary Syndrome: Tachyarrhythmia. Critical Care Cardiology. 2015;Chapter 15:p 54-60. (Japanese) 9. Ashikaga H. Complication of Acute Coronary Syndrome: Bradyarrhythmia. Critical Care Cardiology. 2015;Chapter 16:p 61-70. (Japanese)

Proceedings Reports [PR] 1. Suzuki Y, Fung GSK, Shen Z, Otake Y, Lee O, Ciuffo L, Ashikaga H, Sato Y, Taguchi K. Projection-based motion estimation for cardiac functional analysis with high temporal resolution: a proof-of-concept study with digital phantom experiment. Proc SPIE 10132, Medical Imaging 2017: Physics of Medical Imaging, 1013230;doi:10.1117/12.2254723 2. Kidoh M, Shen Z, Suzuki Y, Ciuffo L, Ashikaga H, Fung GSK, Otake Y, Zimmerman SL, Lima JAC, Higuchi T, Lee O, Sato Y, Becker LC, Fishman EK, Taguchi K. False dyssynchrony: problem with image-based cardiac functional analysis using x-ray computed tomography. Proc SPIE 10132, Medical Imaging 2017: Physics of Medical Imaging, 101321U;doi:10.1117/12.2250257

Editorials [ED] 1. Ashikaga H, Marine JE. Prevention of atrial fibrillation: Another good reason to recommend statins for women? Heart. 2009;95:693-694. 2. Ashikaga H, Blumenthal RS, Jones SR. Utility of ezetimibe. Am J Cardiol. 2009;103:1321-1322. 3. Aronis KN, BergerRD, Ashikaga H. Rotors: How do we know when they are real? Circ Arrhythm Electrophysiol. 2017;10:e005634.

Letters, Correspondence [LT] 1. Ashikaga H, Abreu R, Schneider R. Propranolol administration in a patient with thyroid storm. Ann Intern Med. 2000;132:681-682. 2. Ashikaga H, McVeigh ER, Omens JH. Reply: On the mechanisms of transmural dispersion of myocardial mechanics. J Am Coll Cardiol. 2007;50:650. 3. Zghaib T, Ipek EG, Hansford R, Ashikaga H, Berger RD, Marine JE, Spragg DD, Tandri H, Zimmerman SL, Halperin H, Brancato S, Calkins H, Henrikson C, Nazarian S. Response by Zghaib et al. to Letter Regarding Article, "Standard Ablation Versus Magnetic Resonance Imaging-Guided Ablation in the Treatment of Ventricular Tachycardia". Circ Arrhythm Electrophysiol. 2018;11:e006413.

Media Releases or Interviews [MR] 3/10/15 Media release. Approach That 'Digitizes' Crosstalk Among Heart Cells May Help Locate Epicenters of Dangerous Heart Rhythms. Johns Hopkins Medicine. http://www.hopkinsmedicine.org/news/media/releases/approach_that_digitizes_crosstalk_among_hear t_cells_may_help_locate_epicenters_of_dangerous_heart_rhythms 4/27/15 Media release. MRI May Help Gauge Stroke Risk in Those With Irregular Heartbeat. Johns Hopkins Medicine. US News. http://health.usnews.com/health-news/articles/2015/04/27/mri-may-help-gauge-stroke-risk-in-those- with-irregular-heartbeat 4/27/15 Interviewed by Reporter Wayne Forrest on AuntMinnie.com, “Motion-tracking MRI gives new insight into stroke risk.” http://www.auntminnie.com/index.aspx?sec=ser&sub=def&pag=dis&ItemID=110818 6/1/15 Interviewed by Program Manager Hilary Skolnik at Santa Fe Institute, “Alumni Featured Profile.” https://www.santafe.edu/engage/learn/alumni/hiroshi-ashikaga-fp 11/2/16 Media release. ‘Corkscrew’ Shape of Blood Flow in Heart’s Upper Chamber May Signal Lower Stroke Risk. Johns Hopkins Medicine. http://www.hopkinsmedicine.org/news/media/releases/corkscrew_shape_of_blood_flow_in_hearts_up per_chamber_may_signal_lower_stroke_risk 11/9/16 Media release. Video: This visualization of a human heart could help doctors predict stroke risk. Science. http://www.sciencemag.org/news/2016/11/video-visualization-human-heart-could-help-doctors- predict-stroke-risk 2/15/17 Interviewed by Contributing writer Margit B. Weisgal for Baltimore Sun – Prime Time Living, “An Issue To Take To Heart.” 7 http://marketplace.baltimoresun.com/baltimore-md-features/special-section/Prime-Time-Living/02-15- 2017/Page-4/Story/An-Issue-To-Take-To-Heart 7/17/17 Media release. Quantifying Effectiveness of Treatment for Irregular Heartbeat. Johns Hopkins Medicine. http://www.hopkinsmedicine.org/news/media/releases/quantifying_effectiveness_of_treatment_for_irr egular_heartbeat 2/12/18 Media release. Biomarker Predicts Success of Afib Treatment. Johns Hopkins Medicine. https://www.hopkinsmedicine.org/news/media/releases/biomarker_predicts_success_of_afib_treatmen t

Other Media [OM] 1. Google Scholar: https://scholar.google.com/citations?user=LxvdBmYAAAAJ&hl=en 2. Publications can be downloaded from the laboratory website: http://www.hiroshiashikaga.org/ 3. Video abstract: http://ieeexplore.ieee.org/xpl/articleDetails.jsp?reload=true&arnumber=6832680 4. Github Software repository: https://github.com/ashikagah 5. Ashikaga H, Aguilar-Rodríguez J, Gorsky S, Lusczek E, Marquitti FM, Thompson B, Wu D, Garland J. Modeling the heart as a communication system. arXiv preprint 1410.2169, 2014 https://arxiv.org/abs/1410.2169 6. Ashikaga H, James RG. Hidden structures of information transport underlying spiral wave dynamics. arXiv preprint 1608.06816, 2016. http://arxiv.org/abs/1608.06816 7. Ashikaga H, Asgari-Targhi A. Locating order-disorder phase transition in a cardiac system. arXiv preprint 1708.03990, 2017. https://arxiv.org/abs/1708.03990 8. Ashikaga H, James RG. Inter-scale information flow as a surrogate for downward causation that maintains spiral waves. arXiv preprint 1711.10126, 2017. https://arxiv.org/abs/1711.10126 9. Ashikaga H, Prieto-Castrillo F, Kawakatsu M, Dehghani N. Causal unit of rotors in a cardiac system. arXiv preprint 1712.02203, 2017. https://arxiv.org/abs/1712.02203 10. Aronis AN, Berger RD, Calkins H, Chrispin J, Marine JE, Spragg DD, Tao, S, Tandri T, Ashikaga H. Is human atrial fibrillation stochastic or deterministic? arXiv preprint 1802.01496, 2018. https://arxiv.org/abs/1802.01496 11. Ashikaga H, Aronis AN, Tao S, James RG. Causal scale shift associated with phase transition to human atrial fibrillation. arXiv preprint 1804.10828, 2018. http://arxiv.org/abs/1804.10828 12. Sohn D, Aronis AN, Ashikaga H. Scale-invariant structures of spiral waves. arXiv preprint 1806.10768, 2018. https://arxiv.org/abs/1806.10768 13. Ashikaga H. Speaker, invited lecture, “Causality, Information Flow and Fibrillation.” Integrative Cardiac Dynamics Workshop, Kavli Institute of Theoretical Physics, University of California, Santa Barbara, California. July 11, 2018. http://online.kitp.ucsb.edu/online/cardio18/ashikaga/

FUNDING EXTRAMURAL Funding Research Extramural Funding – Current 6/1/14 – 5/31/19 Improved Targeting and Assessment of Electrophysiology Intervention R01 HL 094610 NIH/NHLBI $4,769,655 PI: Halperin H Role: Co-Investigator: 5%

2/9/16 – 2/9/19 Randomized Evaluation of Atrial Fibrillation Treatment With Focal Impulse and Rotor Modulation Guided Procedures (REAFFIRM) ClinicalTrials.gov Identifier: NCT02274857 Abbott Electrophysiology $116,170 Role: PI: 1%

10/1/16 – 9/30/21 Repolarization Heterogeneity Imaging for Personalized Therapy of Heart Arrhythmia Transatlantic Networks of Excellence 16CVD02 Fondation Leducq 8 $5,442,531 PI: Haissaguerre, M (European PI) and Efimov, I (North American PI) Role: Member: 17%

9/7/17 – 8/31/19 Personalized Risk Prediction of Stroke and Silent Brain Infarction Using Computational Fluid Dynamics (NCT) R56 HL 138429 NIH/NHLBI $250,000 Role: PI: 25%

8/1/18 – 12/31/18 Information-Theoretic Approach to Ventricular Fibrillation Visiting Scholarship University of Bordeaux Initiative of Excellence (IdEX Bordeaux) Role: PI: 100%

Research Extramural Funding – Pending N/A

Research Extramural Funding – Previous 7/1/02 – 5/16/04 In vivo transcoronary rAAV double gene transfer of S16E mutant phospholamban and SERCA2a in cardiomyopathic hamster 0225001Y Postdoctoral Fellowship American Heart Association, Western States Affiliate $92,000 Role: PI: 80%

8/1/12 – 5/31/13 Time-Resolved Cardiac Computed Tomography with Patient Dose Reduction R01 HL 087918 NIH/NHLBI $1,250,000 PI: Taguchi K Role: Co-Investigator: 17%

7/1/13 – 6/30/17 Multi-Modality, Image-Guided Diagnosis and Intervention for Heart Rhythm Disorder Exhibit C-27 Philips Healthcare, Inc. $100,000 Role: PI: 5%

9/4/15 – 8/31/16 Motion-compensated contrast-enhanced cardiac CT for perfusion and angiography R56 HL 125680 NIH/NHLBI $291,524 PI: Taguchi K Role: Co-Investigator: 5%

1/1/16 – 12/31/16 Manipulating Communication Networks of Human Atrial Fibrillation Heart Research Program W.W. Smith Charitable Trust $90,909 Role: PI: 15%

4/1/17 – 9/6/17 Personalized Risk Prediction of Stroke and Silent Brain Infarction Using Computational Fluid Dynamics 9 CV185-582 Brystol-Myers Squibb $119,048 Role: PI: 20%

10/1/16 – 3/31/18 Multicenter AIDS Cohort Study (MACS) - Part B (Baltimore Center) U01 AI 035042 NIH/NIAID $6,932,896 PI: Margolick J Role: Co-Investigator: 10%

7/1/17 – 6/30/19 Randomized Evaluation of Redo Ablation Procedures of Atrial Fibrillation With Focal Impulse and Rotor Modulation Guided Procedures (REDO-FIRM) ClinicalTrials.gov Identifier: NCT02799043 Abbott Electrophysiology $93,136 Role: PI: 1%

Educational Extramural Funding – Not applicable Clinical Extramural Funding – Not applicable System Innovation or Quality Improvement Extramural Funding – Not applicable Other Extramural Funding – Not applicable

INTRAMURAL Funding Research Intramural Funding – Current – Not applicable Research Intramural Funding – Pending – Not applicable Research Intramural Funding – Previous 1/1/14 – 12/31/14 Personalized Stroke Risk Assessment Using Computational Fluid Dynamics Magic that Matters Fund for Cardiovascular Research Johns Hopkins Division of Cardiology and Cardiac Surgery $42,146 Role: PI: 15%

1/1/15 – 12/31/15 Personalized Stroke Risk Assessment Using Computational Fluid Dynamics Zegar Family Foundation Johns Hopkins Heart and Vascular Institute $50,000 Role: PI: 15%

7/1/15 – 6/30/16 Painless Therapy for Sudden Death Discovery Innovation Award Johns Hopkins University Institute of Clinical and Translational Research (ICTR) $50,000 Role: PI: 5%

Educational Intramural Funding – Not applicable Clinical Intramural Funding – Not applicable System Intramural or Quality Improvement Extramural Funding – Not applicable Other Intramural Funding – Not applicable

CLINICAL ACTIVITIES Clinical Focus

10 Dr. Ashikaga’s clinical focus is to improve the understanding of the mechanism of atrial and ventricular fibrillation to develop new and effective interventional strategies to prevent morbidity and mortality.

Certification 1996 Standard ECFMG Certificate (#0-522-486-0, valid indefinitely) 2003 Testamur, Special Competence in Perioperative Transesophageal Echocardiography (PTEeXAM), National Board of Echocardiography 2009 Cardiovascular CT, Level 2 Certification, Society of Cardiovascular Computed Tomography 2010 Nuclear Cardiology, Level 2 Certification

Medical, other state/government licensure 1996-present Japan (License #381284) 2001-2008 California (License #A74161) 2004-present Maryland (License #D61200)

Boards, other specialty certification 2000-present Diplomate, American Board of Internal Medicine (Internal Medicine, recertified 2010) 2010-present Diplomate, American Board of Internal Medicine (Cardiovascular Disease) 2012-present Diplomate, American Board of Internal Medicine (Clinical Cardiac Electrophysiology)

Clinical (Service) Responsibilities 2012-present Attending Physician, Cardiac Arrhythmia Service Specialty: Clinical Cardiac Electrophysiology 20-60% effort (outpatient/inpatient procedures) at Johns Hopkins Hospital, Baltimore, Maryland 20% effort (10% outpatient clinic + 10% outpatient/inpatient procedures) at Greater Baltimore Medical Center, Towson, Maryland

Clinical Productivity 2017 4,880 Work RVU (40% clinical effort)

Clinical Draw from outside local/regional area – Data not available Membership in or examiner for specialty board – Not applicable Clinical Program Building / Leadership – Not applicable Clinical Demonstration Activities to external audience, on or off campus – Not applicable Development of nationally/internationally recognized clinical standard of care – Not applicable

EDUCATIONAL ACTIVITIES Educational Focus Dr. Ashikaga’s educational focus is to train the next generation of scientists in interdisciplinary approaches to studying the heart at the intersection of applied mathematics, statistical physics and neuroscience.

Teaching Classroom instruction – Not applicable Clinical instruction JHMI/Regional 2012-present Attending physician, Clinical fellows in Cardiovascular Disease; Inpatient cardiology subspecialty consultation service. 8-10 weeks each year, Johns Hopkins SOM 2012-present Attending physician, Clinical fellows in Clinical Cardiac Electrophysiology; Electrophysiology procedure supervision. 20-30 weeks each year, Johns Hopkins SOM National Not applicable International Not applicable

CME instruction – Not applicable Workshops /seminars – Not applicable

Mentoring 11 Pre-doctoral Advisees /Mentees 7/04-6/05 Steven Mickelsen. M.D. candidate, University of New Mexico. Howard Hughes Medical Institute. Publications: Original Research [OR] 6, 7 Present position: Assistant Professor of Medicine, University of Iowa 7/10-7/16 Farhad Pashakhanloo. Ph.D. candidate, Johns Hopkins University (JHU) Department of Biomedical Engineering. Publications: Original Research [OR] 29, 32, 38, 45 Present position: Postdoctoral fellow, JHU Department of Biomedical Engineering 6/14-11/14 Tsubasa Okazaki. M.D. candidate, Tokyo Medical and Dental University, Japan. Present position: Resident physician, Tokyo Medical and Dental University affiliated hospital, Japan 3/15-9/15 Tomohiro Otani. Ph.D. candidate, Osaka University School of Engineering, Japan. Publications: Original Research [OR] 44 Present position: Assistant Professor, Osaka University School of Engineering, Japan 2/16-2/17 Ameneh Asgari-Targhi. Ph.D. candidate, University of Glasgow School of Mathematics and Statistics, UK. Publications: Original Research [OR] 65 Present position: Postdoctoral fellow, Brigham and Women’s Hospital, Boston, Massachusetts 1/17-12/17 Arun Chandra. M.D. candidate, JHU. 12/16-present Eric Sung. M.D.-Ph.D. candidate in Biomedical Engineering, JHU. 8/17-present Daniel Sohn. B.S. candidate in Computer Science, JHU. 9/18-present Eric Xie. M.D. candidate, JHU.

Post-doctoral Advisees /Mentees 7/13-6/15 Yuko Inoue, M.D., Ph.D. Postdoctoral fellow, JHU. Publications: Original Research [OR] 28, 35, 39, 51, 59, 67, 71 Award: 2014 Clinical Cardiology Minority Travel Grant, American Heart Association Present position: Attending physician, Department of Arrhythmia, National Cerebral and Cardiovascular Center, Osaka, Japan 4/14-3/15 Abdullah Alissa, M.D. Postdoctoral fellow, JHU. Publications: Original Research [OR] 35, 39, 44 Present position: Resident Physician, Department of Medicine, University of Maryland 2/16-6/18 Luisa A. Ciuffo, M.D. Postdoctoral fellow, JHU Publications: Original Research [OR] 55, 56, 57, 58, 63, 67, 71, 72; Proceedings Reports [PR] 1, 2 7/15-12/18 Susumu Tao, M.D., Ph.D. Postdoctoral fellow, JHU Publications: Original Research [OR] 55, 57, 58, 67, 70 Award: 2017-2018 Heart Rhythm Society Research Fellowship Award in Cardiac Pacing and Electrophysiology supported by an education grant from St. Jude Medical 7/17-present Konstantinos N. Arnois, M.D. Postdoctoral fellow, JHU Publications: Original Research [OR] 64, 70; Editorials [ED] 3; Other Media [OM] 10, 11, 12 Award: Travel Award, Dynamics Days (https://www.colorado.edu/amath/ddays2018). January 4-6, 2018. Denver, Colorado

Thesis committees – Not applicable Educational Program Building / Leadership – Not applicable Educational Demonstration Activities to external audience – Not applicable

RESEARCH ACTIVITIES Research Focus Dr. Ashikaga is a cardiac electrophysiologist with expertise in information theory for complex systems, mathematical modeling, medical imaging physics, and continuum mechanics. Dr. Ashikaga’s research specializes in interdisciplinary approaches to studying the heart at the intersection of applied mathematics, statistical physics and neuroscience. His laboratory focuses on elucidating fundamental mechanisms of fibrillation, and exploiting that knowledge to develop and evaluate novel therapeutic technologies. Dr. Ashikaga has a unique advantage for interdisciplinary research due to his ability to understand and speak the language of biological, quantitative and clinical sciences. His publication record demonstrates examples of successful interdisciplinary collaborations.

12 Research Program Building / Leadership 2014-present Director, Laboratory of Unconventional Electrophysiology. I am currently running two research programs in my multi-disciplinary research laboratory: 1) Artificial Intelligence for Sudden Cardiac Death The objective is to develop and validate artificial intelligence technologies to predict and prevent sudden cardiac death 2) Personalized Risk Prediction Using Advanced Imaging Technology The objective is to identify biomarkers using advanced imaging technology to predict patient-specific risk for irregular heart rhythm, stroke, cognitive impairment and sudden death

Research Demonstration Activities – Not applicable

Inventions, Patents, Copyrights 10/27/16 Ashikaga H, Otani T. Patient-specific Virtual Intervention Laboratory to Prevent Stroke. WO/2016/172206.

Technology Transfer Activities – Not applicable

SYSTEM INNOVATION AND QUALITY IMPROVEMENT ACTIVITIES – Not applicable

ORGANIZATIONAL ACTIVITIES Institutional Administrative Appointments 2015-2016 Ph.D. Candidate Selection Group, Computational Biology Section, Department of Biomedical Engineering, Johns Hopkins University School of Medicine

Editorial Activities Editorial Board appointments – Not applicable Journal peer review activities 2004-present Journal of the American College of Cardiology (Elite Reviewer in 2006) 2004-present American Journal of Physiology – Heart and Circulatory Physiology 2005-present Journal of Applied Physiology 2006-present Experimental Biology and Medicine 2007-present Hypertension 2008-present JACC Cardiovascular Imaging 2008-present American Journal of Cardiology 2008-present Circulation 2009-present IEEE Transactions of Medical Imaging 2009-present Internal Medicine 2010-present Circulation Research 2010-present Heart Rhythm 2011-present Circulation Cardiovascular Imaging 2014-present Journal of the American Heart Association 2014-present Journal of the American Medical Association 2014-present Journal of the Royal Society Interface 2015-present PLOS Computational Biology 2015-present PLOS ONE 2016-present JACC Clinical Electrophysiology 2017-present Magnetic Resonance in Medicine 2018-present Complexity 2018-present Scientific Reports 2018-present Journal of Cardiovascular Magnetic Resonance

Other peer review activities 2017-present Grant peer-reviewer, Medical Research Council (MRC), Swindon, United Kingdom 3/13/2017 Clinical Research Training Fellowship.. 13 2018-present Grant peer-reviewer, Engineering and Physical Sciences Research Council (EPSRC), Swindon, United Kingdom 5/1/2018 Overseas Travel Grants. 2018-present Grant peer-reviewer, American Heart Association (AHA) 5/15/2018 Transformational Project Award, Clinical-Population Sciences. Radiology and Imaging Clinical. Dallas, TX. 2018-present Grant peer-reviewer, National Institutes of Health (NIH) 7/26-27/2018 Special Emphasis Panel, RFA-HL-17-015, Bold New Bioengineering Methods and Approaches for Heart, Lung, Blood and Sleep Disorders and Diseases (R21). Washington, DC.

Professional Societies 2002-present Member, American Heart Association (AHA) 2018-present Fellow of the American Heart Association (FAHA) 2008-present Member, Heart Rhythm Society (HRS) 2012-present Fellow of the Heart Rhythm Society (FHRS) 2015-present Member, Society for Industrial and Applied Mathematics (SIAM) 2016-present Member, American Physical Society (APS)

Conference Organizer – Not applicable

Session Chair JHMI/Regional – Not applicable National 5/16 Session chair, Heart Rhythm Society Annual Scientific Sessions, San Francisco, California.

International – Not applicable

Consultantships – Not applicable

RECOGNITION Awards, Honors 1997 Resident Scholarship, Japan-North America Medical Exchange Foundation 2002 Postdoctoral Fellowship, American Heart Association 2003 Travel Award, American Federation for Medical Research 2003 Travel Award, American College of Cardiology 2006 Fellowship Award, Seiichi Shimomura/Astellas USA Foundation 2006 Finalist and Honorable mention, Young Investigator Award, Heart Rhythm Society 2006 Elite Reviewer, Journal of the American College of Cardiology 2010 Howard S. Silverman Award, for originality and creativity in medical research, Division of Cardiology, Johns Hopkins University School of Medicine 2011 Winner, Young Investigator Award, Asia-Pacific Heart Rhythm Society 2011 Finalist, Melvin Judkins Young Clinical Investigator Award, American Heart Association 2014 Fellow of the Heart Rhythm Society 2018 Fellow of the American Heart Association

Invited Talks JHMI/Regional 1/28/15 Speaker, Cardiology Grand Rounds, Johns Hopkins University School of Medicine, “New Approach to Atrial Fibrillation,” Baltimore, Maryland 5/23/18 Speaker, Cardiology Grand Rounds, Johns Hopkins University School of Medicine, “Fibrillation: What Cardiologists Can Learn from Neuroscientists,” Baltimore, Maryland

National: 12/10/03 Speaker, invited lecture, Division of Cardiovascular Medicine, Stanford University School of Medicine, “Transmural left ventricular mechanics underlying torsional recoil during relaxation,” Stanford, California 14 12/20/03 Speaker, invited lecture, Laboratory of Cardiac Energetics, National Heart, Lung and Blood Institute, National Institutes of Health, “Transmural left ventricular mechanics underlying torsional recoil during relaxation,“ Bethesda, Maryland 10/15/05 Speaker, Session in Cardiac Biomechanics and Mechanobiology, Annual Meeting, Biomedical Engineering Society, “Ventricular Sheet Structure and Mechanics in Chronic Volume Overload,” Baltimore, Maryland 11/7/11 Speaker, Cardiology Grand Rounds, Department of Internal Medicine, University of Michigan Medical School, “Role of Heteroheneous Zone in Ventricular Arrhythmogenesis,” Ann Arbor, Michigan 12/13/11 Speaker, invited lecture, Comprehensive Arrhythmia Research and Management Center (CARMA), University of Utah, “Role of Heteroheneous Zone in Ventricular Arrhythmogenesis,” Salt Lake City, Utah 1/15/12 Speaker, invited lecture, Cardiac Arrhythmia Center, University of California, Los Angeles, “Role of Heteroheneous Zone in Ventricular Arrhythmogenesis,” Los Angeles, California 11/8/11 Speaker, invited lecture, Sanofi-Aventis, “Role of Intravenous Amiodarone for Management of Ventricular Tachycardia/Fibrillation,” Orlando, Florida 10/16/14 Speaker, Dynamical Systems Seminar, Department of Applied Mathematics, University of Colorado, Boulder, “Information Theory of the Heart,” Boulder, Colorado 1/8/15 Speaker, invited lecture, Amgen, “New Approach to Atrial Fibrillation,” South San Francisco, California 10/13/16 Speaker, Complexity in Medicine and Healthcare Short Course at Santa Fe Institute, “Information Theory of the Heart”, Santa Fe, New Mexico 11/13/16 Speaker, invited lecture, Bristol-Myers Squibb, “Information Theory of the Heart,” New Orleans, Louisiana 7/11/18 Speaker, invited lecture, Integrative Cardiac Dynamics Workshop, Kavli Institute of Theoretical Physics, University of California, Santa Barbara, “Causality, Information Flow and Fibrillation,” Santa Barbara, California http://online.kitp.ucsb.edu/online/cardio18/ashikaga/

International: 2/18/06 Speaker, invited lecture, Laboratory of Computational Biomechanics, Graduate School of Frontier Sciences, University of Tokyo, “Ventricular Fiber/Sheet Structure and Cardiac Electromechanics,” Tokyo, Japan 8/14/06 Speaker, invited lecture, Department of Cardiovascular Medicine, Kurume University School of Medicine, “Clinical Applications of Cardiovascular CT/MRI,” Fukuoka, Japan 11/5/07 Speaker, invited lecture, Annual Yushima Arrhythmia Conference, “Role of Advanced Imaging in Clinical Cardiac Electrophysiology,” Tokyo, Japan 5/28/11 Speaker, invited lecture, 84th Annual Scientific Meeting, Japanese Society of Ultrasonics in Medicine, “Myofiber Orientation and Ventricular Mechanics,” and “Accuracy of Echocardiography-Based Quantification of Cardiac Mechanics,” Tokyo, Japan 9/8/13 Speaker, invited lecture, 12th Annual Surugadai International Symposium and Joint Usage/Research Program of the Medical Research Institute International Symposium, “Advanced Imaging Science for Cardiac Arrhythmia,” Tokyo, Japan 11/20/13 Speaker, invited lecture, Section of Cardiac Arrhythmia, Tokyo Medical and Dental University School of Medicine, “Advanced Imaging Science for Cardiac Arrhythmia,” Tokyo, Japan 11/25/13 Speaker, invited lecture, Division of Arrhythmia, National Cerebral and Cardiovascular Center, “Advanced Imaging Science for Cardiac Arrhythmia,” Osaka, Japan 5/11/14 Speaker, invited lecture, Department of Cardiovascular Research, Research Institute of Environmental Medicine (RIEM), Nagoya University, “Advanced Imaging Science for Cardiac Electrophysiology,” Nagoya, Japan 10/24/15 Speaker, invited lecture, Biomechanics Laboratory, Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University “Mathematical Approach to Heart Disease,” Osaka, Japan 10/27/15 Speaker, invited lecture, Imaging-based Computational Biomedicine Laboratory, Graduate School of Information Science, Nara Institute of Science and Technology (NAIST), “Mathematical Approach to Heart Disease,” Nara, Japan 9/15/17 Speaker, invited lecture, 10th Annual Scientific Sessions of the Asia-Pacific Heart Rhythm Society, “Data- Driven Modeling for Cardiac Fibrillation,” Yokohama, Japan 9/29/17 Speaker, invited lecture, Department of Mathematics, University of Exeter, “Complex Systems Approach to Atrial Fibrillation,” Exeter, United Kingdom

15 4/5/18 Speaker, Interfaces: Aquitaine scientific symposium for digital sciences, INRIA (Institut national de recherche en informatique et en automatique), “What’s Common Among Statistical Physics, Consciousness, Artificial Intelligence, and Atrial Fibrillation?” Bordeaux, France 5/3/18 Speaker, invited lecture, IHU Liryc, Electrophysiology and Heart Modeling Institute (L'institut de rythmologie et modélisation cardiaque), Université de Bordeaux, “Phase Transition, Renormalization, and Fibrillation,” Bordeaux, France 8/10/18 Speaker, invited lecture, Biomechanical Systems Study Group, Division of Bioengineering (A-TS 02-09), Japan Society of Mechanical Engineers, Osaka University School of Engineering, “Left Atrial Function as a Biomarker for Personalized Treatment,” Osaka, Japan 8/17/18 Speaker, invited lecture, Institute of Industrial Science, University of Tokyo Graduate School of Information Science and Technology, “Phase Transition and Sudden Death,” Tokyo, Japan

Visiting Professorships 1/18-present Visiting Scholar, IHU Liryc, Electrophysiology and Heart Modeling Institute (L'institut de rythmologie et modélisation cardiaque), Université de Bordeaux, France (https://www.ihu-liryc.fr/) 6/18-7/18 Visiting Scholar, Kavli Institute of Theoretical Physics, University of California, Santa Barbara, California (https://www.kitp.ucsb.edu/)

OTHER PROFESSIONAL ACCOMPLISHMENTS – Not applicable