DOCSLIB.ORG

Minireview Genetic Transformation in Aspergilli

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Minireview Genetic Transformation in Aspergilli Indian Journal of Biochemistry & Biophysics Vol. 41, October 2004, pp. 205-215 Minireview Genetic transformation in Aspergilli: Tools of the trade V Lakshmi Prabha and N S Punekar* Biotechnology Group, School of Biosciences and Bioengineering, Indian Institute of Technology, Bombay, Mumbai 400 076 India Received 6 May 2004; revised 19 August 2004 DNA-mediated transformation is a powerful tool that allows the introduction of specific genetic changes in an organism. Transformation of Aspergilli, acclaimed for their wide use in the industry, has been possible for about two decades now. Several basic and applied problems related to fungal biology have been addressed using this technique. Nonetheless, new markers and strategies for transformation are still being developed for these filamentous fungi. Different methods and markers that are currently available for the transformation of Aspergilli are summarized here. The review also brings out the importance of these transformation systems in analyzing fungal gene function. Aspects of Aspergillus niger transformation are selectively emphasized. Keywords: Aspergilli, Aspergillus niger, transformation strategies, genetic markers, transformation vectors, gene function Introduction arduous, due to high melanin content of its cell wall; Aspergilli are of immense economic and medicinal and b) excessive clumping of spores occurs during value and a better understanding of their basic germination2. metabolic processes would enable the control and The first report of transformation of a filamentous treatment of pathogenic species and in the industrial fungus appeared in 1979 when DNA was introduced scenario help in rationalized strain improvement1. into protoplasts of Neurospora crassa3. Genetic transformation is an important tool that Transformation of A. nidulans that followed shortly allows one to address questions relating to afterward4 ushered in an era that has seen rapid metabolism and gene expression as well as the advancement in the understanding of these organisms. regulation of these processes. In Aspergilli that Although protoplast transformation remains one of reproduce predominantly through asexual mode it is the most commonly adopted protocols, several other of further value as it provides a means of introducing methods have been developed since then. They are specific genetic changes. i) Agrobacterium-mediated gene transfer; ii) In filamentous fungi, genetic transformation is electroporation of young germlings; iii) particle usually achieved by integration of the introduced bombardment; and iv) DNA uptake by lithium acetate DNA into the genome of the organism. Integration is treated cells. Salient features of these methods are mostly through heterologous recombination; enumerated below. A review of recent literature on homologous recombination in fungi occurs only at the different markers used/available to transform low frequencies. The lower transformation filamentous fungi is presented with an emphasis on efficiencies (in relation to yeast or E. coli) observed in Aspergilli. Although there are a number of patents in filamentous fungi may largely be ascribed to their this general field, this review is largely confined to tough cell wall. Although the problems encountered literature in the public domain barring few exceptions. in transforming Aspergilli are not unique, some of these species are more difficult to transform, than the Entry of incoming DNA others. For instance, in A. niger — a) protoplasting is Transformation of protoplasts ______________ The most routinely used protocol for the *Author for correspondence transformation of filamentous fungi involves the E-mail: [email protected] preparation of protoplasts followed by DNA uptake Tel: 91-22-2576 7775 mediated either by CaCl2/PEG treatment or more Fax: 91-22-2572 3480 recently by electroporation5,6. Protoplasts are prepared Abbreviations: 5-FOA, 5-fluoroorotic acid; GRAS, generally regarded as safe; OMP, orotidine-5’-monophosphate; PCR, from swollen conidia or from young mycelia by polymerase chain reaction; PEG, polyethylene glycol. enzymatic digestion of their cell walls. For 206 INDIAN J. BIOCHEM. BIOPHYS.,VOL. 41, OCTOBER 2004 transformation, protoplasts in osmotic medium interest (gene to be introduced into the fungus) and a containing CaCl2 are incubated with exogenous DNA fungal selectable marker are cloned between the and PEG (aids transformation by bringing the DNA direct repeats in an E. coli-compatible plasmid and and protoplasts together). Highest transformation introduced into Agrobacterium to generate the efficiencies are reported to coincide with germ tube T-DNA, while the necessary virulence functions are emergence in A. nidulans7. provided in trans from a Ti plasmid devoid of T-DNA15. Electroporation Agrobacterium-mediated DNA transfer in Transformation by electroporation involves filamentous fungi is possible with both conidia and 16,17 exposing cells to short duration high voltage electrical protoplasts . The transforming DNA usually pulses in the presence of exogenous DNA. The integrates randomly into the host genome. However, electrical pulses induce a reversible permeabilization this technique has also been used to mediate gene of the plasma membrane and transient pores are targeting by homologous recombination in 18 formed enabling the DNA to enter the cell8. A. awamori . Electroporation was initially used for the transformation of protoplasts as an alternative to the Other approaches 6 CaCl2/PEG method . It was later demonstrated that Transformation of lithium acetate treated cells, germinating conidia could also be transformed using routinely used for yeast and also demonstrated in this technique. Although the first reports of the N. crassa, has not been reported for Aspergilli. procedure included the use of a mild pretreatment However, direct transformation of lysine requiring with β-glucuronidase to weaken the cell wall9, mutants of Aspergillus ochraceus could be achieved transformation of swollen conidia without the need by subjecting the mutant conidia to starvation for pretreatment has been reported for A. niger10, conditions for 15 days and then treating them with A. nidulans11 and A. fumigatus12. DNA from the wild-type strain19. This method nevertheless is not commonly used. Biolistic transformation Particle bombardment as a technique for DNA Comparison of different methods delivery was developed in the late 1980's13. In this The methods described above have their own pros method, the DNA is first precipitated on small (1 μm) and cons. Transformation of protoplasts necessitates gold or tungsten particles, which are accelerated their isolation, which is a laborious procedure towards the target in partial vacuum. After particle requiring great care in experimentation. The penetration, the DNA dissociates from the particles competence achieved by the protoplasts varies vastly and moves to the nucleus, where it gets integrated into with the batch of mycolytic enzyme used. A 100-fold slump in transformation frequency on varying the the genome. A. nidulans is perhaps the only 20 Aspergillus species that has been transformed using batch of enzyme used has been reported . This this method14. This method is generally more useful method is also limited due to by the low and often for the transformation of fungi that are refractory to variable regeneration frequency of protoplasts. other methods. Nonetheless, protoplast transformation remains the most commonly adopted procedure. Electroporation is simpler and provides a good alternative to protoplast Agrobacterium-mediated transformation transformation; it, however, requires a specialized This method exploits the innate virulence apparatus and offers no advantage in terms of (conferred by the Ti plasmid) of Agrobacterium to improved transformation efficiency. Biolistic achieve transformation. The bacterium is co- transformation is expensive and again, requires cultivated with the host to be transformed in the elaborate apparatus. The method is, however, presence of virulence-inducing molecules like important in those fungi that are refractory to other acetosyringone. This leads to the mobilization of transformation protocols. T-DNA, a region of the Ti plasmid flanked by imperfect 24 bp direct repeats. The T-DNA Transformation mediated by Agrobacterium is the subsequently enters the host cell and gets integrated most recent and perhaps the simplest method of all. It into the genome. In the binary system, the gene of is often more effective than the conventional methods PRABHA & PUNEKAR: GENETIC TRANSFORMATION IN ASPERGILLI 207 of protoplast transformation. For instance, use of Nutritional markers Agrobacterium improved the transformation An appropriate mutant recipient strain must be frequency of A. awamori up to 600-fold as compared available to employ nutritional markers. Selection in with protoplast transformation16. A comparison of the case of nutritional markers is based on four different transformation methods has been complementation of a nutritional defect by 22 reported for Aspergillus giganteus. Agrobacterium- introduction of the wild type allele . The amdS gene mediated transformation with a 140-fold higher is the only exception and it confers the ability to transformation frequency than protoplast utilize acetamide as the sole carbon/nitrogen source in 23 transformation was adjudged the best21. The ease of a wild type background . Table 1 lists the nutritional the protocol coupled with the wide host range of selectable markers employed
Load more

Recommended publications
  • Gaseous Chlorine Dioxide
    Bacterial Endospores Mycobacteria Non-Enveloped Viruses Fungi Gram Negative Bacteria Gram Positive Bacteria Enveloped, Lipid Viruses Blakeslea trispora 28 E. coli O157:H7 G5303 1 Bordetella bronchiseptica 8 E. coli O157:H7 C7927 1 Brucella suis 30 Erwinia carotovora (soft rot) 21 Burkholderia mallei 36 Franscicella tularensis 30 Burkholderia pseudomallei 36 Fusarium sambucinum (dry rot) 21 Campylobacter jejuni 39 Fusarium solani var. coeruleum (dry rot) 21 Clostridium botulinum 32 Helicobacter pylori 8 Corynebacterium bovis 8 Helminthosporium solani (silver scurf) 21 Coxiella burneti (Q-fever) 35 Klebsiella pneumonia 3 E. coli ATCC 11229 3 Lactobacillus acidophilus NRRL B1910 1 E. coli ATCC 51739 1 Lactobacillus brevis 1 E. coli K12 1 Lactobacillus buchneri 1 E. coli O157:H7 13B88 1 Lactobacillus plantarum 5 E. coli O157:H7 204P 1 Legionella 38 E. coli O157:H7 ATCC 43895 1 Legionella pneumophila 42 E. coli O157:H7 EDL933 13 Leuconostoc citreum TPB85 1 The Ecosense Company (844) 437-6688 www.ecosensecompany.com Page 1 of 6 Leuconostoc mesenteroides 5 Yersinia pestis 30 Listeria innocua ATCC 33090 1 Yersinia ruckerii ATCC 29473 31 Listeria monocytogenes F4248 1 Listeria monocytogenes F5069 19 Adenovirus Type 40 6 Listeria monocytogenes LCDC-81-861 1 Calicivirus 42 Listeria monocytogenes LCDC-81-886 19 Canine Parvovirus 8 Listeria monocytogenes Scott A 1 Coronavirus 3 Methicillin-resistant Staphylococcus aureus 3 Feline Calici Virus 3 (MRSA) Foot and Mouth disease 8 Multiple Drug Resistant Salmonella 3 typhimurium (MDRS) Hantavirus 8 Mycobacterium bovis 8 Hepatitis A Virus 3 Mycobacterium fortuitum 42 Hepatitis B Virus 8 Pediococcus acidilactici PH3 1 Hepatitis C Virus 8 Pseudomonas aeruginosa 3 Human coronavirus 8 Pseudomonas aeruginosa 8 Human Immunodeficiency Virus 3 Salmonella 1 Human Rotavirus type 2 (HRV) 15 Salmonella spp.
    [Show full text]
  • The Antifungal Protein AFP from Aspergillus Giganteus Prevents Secondary Growth of Different Fusarium Species on Barley
    Appl Microbiol Biotechnol DOI 10.1007/s00253-010-2508-4 BIOTECHNOLOGICALLY RELEVANT ENZYMES AND PROTEINS The antifungal protein AFP from Aspergillus giganteus prevents secondary growth of different Fusarium species on barley Hassan Barakat & Anja Spielvogel & Mahmoud Hassan & Ahmed El-Desouky & Hamdy El-Mansy & Frank Rath & Vera Meyer & Ulf Stahl Received: 23 December 2009 /Revised: 9 February 2010 /Accepted: 10 February 2010 # Springer-Verlag 2010 Abstract Secondary growth is a common post-harvest Aspergillus giganteus. This protein specifically and at low problem when pre-infected crops are attacked by filamen- concentrations disturbs the integrity of fungal cell walls and tous fungi during storage or processing. Several antifungal plasma membranes but does not interfere with the viability approaches are thus pursued based on chemical, physical, of other pro- and eukaryotic systems. We thus studied in or bio-control treatments; however, many of these methods this work the applicability of AFP to efficiently prevent are inefficient, affect product quality, or cause severe side secondary growth of filamentous fungi on food stuff and effects on the environment. A protein that can potentially chose, as a case study, the malting process where naturally overcome these limitations is the antifungal protein AFP, an infested raw barley is often to be used as starting material. abundantly secreted peptide of the filamentous fungus Malting was performed under lab scale conditions as well as in a pilot plant, and AFP was applied at different steps Hassan Barakat and Anja Spielvogel equally contributed to this work. during the process. AFP appeared to be very efficient against the main fungal contaminants, mainly belonging to H.
    [Show full text]
  • The Potential Use of the Culture Filtrate of an Aspergillus Niger Strain in the Management of Fungal Diseases of Grapevine Egy A
    Original scientific paper DOI: /10.5513/JCEA01/21.4.2942 Journal of Central European Agriculture, 2020, 21(4), p.839-850 The potential use of the culture filtrate of an Aspergillus niger strain in the management of fungal diseases of grapevine Egy Aspergillus niger törzs fermentlevének lehetséges felhasználása a szőlő gombás betegségeinek megelőzésére Xénia PÁLFI1, Zoltán KARÁCSONY1 (✉), Anett CSIKÓS2, Ottó BENCSIK3, András SZEKERES3, Zsolt ZSÓFI4, Kálmán Zoltán VÁCZY1 1 Eszterházy Károly University, Food and Wine Research Institute, H-3300 Eger, Leányka utca 6., Hungary 2 University of Pannonia, Georgikon Faculty, Institute of Plant Protection, H-8360 Keszthely, Deák Ferenc utca 16., Hungary 3 University of Szeged, Faculty of Science and Informatics, Department of Microbiology, H-6726 Szeged, Közép fasor 52., Hungary 4 Eszterházy Károly University, Department of Viticulture and Oenology, H-3300 Eger, Leányka utca 6., Hungary ✉ Corresponding author: [email protected] Received: June 22, 2020; accepted: August 5, 2020 ABSTRACT The present study was aimed to examine the use of culture filtrates of defensin-producing fungal strains against the most important fungal and pseudofungal infections of grapevine as an environmentally sound alternative of chemical control. Twenty-seven isolates of five defensin-producing fungal species were tested for antifungal activity. Four isolates (one Aspergillus giganteus and three Aspergillus niger) decreased the viability of the test organism successfully. The isolate with the highest antifungal efficacy (Aspergillus niger SZMC2759) was subjected to detailed investigation. The culture filtrate of the strain was effective against epicuticular mycelia and conidia ofBotrytis cinerea, Guignardia bidwellii and Erysiphe necator, but not against subcuticular mycelia andPlasmopara viticola cells.
    [Show full text]
  • Genetic Engineering of Fungal Cells-Margo M
    BIOTECHNOLOGY- Vol III - Genetic Engineering of Fungal Cells-Margo M. Moore GENETIC ENGINEERING OF FUNGAL CELLS Margo M. Moore Department of Biological Sciences, Simon Fraser University, Burnaby, Canada Keywords: filamentous fungi, transformation, protoplasting, Agrobacterium, promoter, selectable marker, REMI, transposon, non-homologous end joining, homologous recombination Contents 1. Introduction 1.1. Industrial importance of fungi 1.2. Purpose and range of topics covered 2. Generation of transforming constructs 2.1. Autonomously-replicating plasmids 2.2. Promoters 2.2.1. Constitutive promoters 2.2.2. Inducible promoters 2.3. Selectable markers 2.3.1. Dominant selectable markers 2.3.2. Auxotropic/inducible markers 2.4. Gateway technology 2.5. Fusion PCR and Ligation PCR 3. Transformation methods 3.1. Protoplast formation and CaCl2/ PEG 3.2. Electroporation 3.3. Agrobacterium-mediated Ti plasmid 3.4. Biolistics 3.5. Homo- versus heterokaryotic selection 4. Gene disruption and gene replacement 4.1. Targetted gene disruption 4.1.1. Ectopic and homologous recombination 4.1.2. Strains deficient in non-homologous end joining (NHEJ) 4.1.3. AMT and homologous recombination 4.1.4. RNA interference 4.2. RandomUNESCO gene disruption – EOLSS 4.2.1. Restriction enzyme-mediated integration (REMI) 4.2.2. T-DNA taggingSAMPLE using Agrobacterium-mediated CHAPTERS transformation (AMT) 4.2.3. Transposon mutagenesis & TAGKO 5. Concluding statement Glossary Bibliography Biographical Sketch Summary Filamentous fungi have myriad industrial applications that benefit mankind while at the ©Encyclopedia of Life Support Systems (EOLSS) BIOTECHNOLOGY- Vol III - Genetic Engineering of Fungal Cells-Margo M. Moore same time, fungal diseases of plants cause significant economic losses.
    [Show full text]
  • Sniper Disinfectant Comparison.Xlsx
    Distributed by FULL CIRCLE ENTERPRISES, LLC 4334 E Washington Blvd Commerce CA 90023 N O N C O R R O S I V E S C OR R O S I V ES Tel: 877-572-4725 Buffered Name Brands Causes Skin Burns. Breathing Masks Required. Email: [email protected] Chlorine Dioxide Amazing what's Those that can be used on hard surfaces, The Disinfectant that "Mild enough to wash your hands in" still living Wipe Off REQUIRED. Can NOT be left on Surface "Kills like a 'corrosive' but mild No Clean Up Necessary AAFTER Treatment enough to wash your hands in" If it's important to kill the germs then why not More than kill them all? 300 MILLION & See our "WATCH THE 9's" Article some are very scary! Bacteria Acinetobacter Calcoaceticus Blakeslea trispora Bordetella bronchiseptica Brucella suis Burkholderia mallei Burkholderia cepacia Burkholderia pseudomallei Campylobacter jejuni Clostridium botulinum Clostridium difficile Corynebacterium bovis Corynebacterium diphtheriae Coxiella burneti (Q-fever) Enterococcus faecalius Enterobacter Aerogenes E. coli ATCC 11229 E. coli ATCC 51739 E. coli K12 E. coli O157:H7 13B88 E. coli O157:H7 204P E. coli O157:H7 ATCC 43895 E. coli O157:H7 EDL933 E. coli O157:H7 G5303 E. coli O157:H7 C7927 Erwinia carotovora (soft rot) Franscicella tularensis Fusarium sambucinum (dry rot) Fusarium solani var. coeruleum (dry rot) Helicobacter pylori Helminthosporium solani (silver scurf) Klebsiella pneumonia Klebsiella pneumonia NDM 1 Positive Klebsiella pneumonia Carbapenem Resistant Lactobacillus acidophilus NRRL B1910 Lactobacillus brevis Lactobacillus
    [Show full text]
  • Lists of Names in Aspergillus and Teleomorphs As Proposed by Pitt and Taylor, Mycologia, 106: 1051-1062, 2014 (Doi: 10.3852/14-0
    Lists of names in Aspergillus and teleomorphs as proposed by Pitt and Taylor, Mycologia, 106: 1051-1062, 2014 (doi: 10.3852/14-060), based on retypification of Aspergillus with A. niger as type species John I. Pitt and John W. Taylor, CSIRO Food and Nutrition, North Ryde, NSW 2113, Australia and Dept of Plant and Microbial Biology, University of California, Berkeley, CA 94720-3102, USA Preamble The lists below set out the nomenclature of Aspergillus and its teleomorphs as they would become on acceptance of a proposal published by Pitt and Taylor (2014) to change the type species of Aspergillus from A. glaucus to A. niger. The central points of the proposal by Pitt and Taylor (2014) are that retypification of Aspergillus on A. niger will make the classification of fungi with Aspergillus anamorphs: i) reflect the great phenotypic diversity in sexual morphology, physiology and ecology of the clades whose species have Aspergillus anamorphs; ii) respect the phylogenetic relationship of these clades to each other and to Penicillium; and iii) preserve the name Aspergillus for the clade that contains the greatest number of economically important species. Specifically, of the 11 teleomorph genera associated with Aspergillus anamorphs, the proposal of Pitt and Taylor (2014) maintains the three major teleomorph genera – Eurotium, Neosartorya and Emericella – together with Chaetosartorya, Hemicarpenteles, Sclerocleista and Warcupiella. Aspergillus is maintained for the important species used industrially and for manufacture of fermented foods, together with all species producing major mycotoxins. The teleomorph genera Fennellia, Petromyces, Neocarpenteles and Neopetromyces are synonymised with Aspergillus. The lists below are based on the List of “Names in Current Use” developed by Pitt and Samson (1993) and those listed in MycoBank (www.MycoBank.org), plus extensive scrutiny of papers publishing new species of Aspergillus and associated teleomorph genera as collected in Index of Fungi (1992-2104).
    [Show full text]
  • Identification and Characterization of an Antifungal Protein, Afafpr9, Produced by Marine-Derived Aspergillus Fumigatus R9 Qi Rao†, Wenbin Guo†, and Xinhua Chen*
    J. Microbiol. Biotechnol. (2015), 25(5), 620–628 http://dx.doi.org/10.4014/jmb.1409.09071 Research Article Review jmb Identification and Characterization of an Antifungal Protein, AfAFPR9, Produced by Marine-Derived Aspergillus fumigatus R9 Qi Rao†, Wenbin Guo†, and Xinhua Chen* Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, P.R. China Received: September 23, 2014 Revised: October 29, 2014 A fungal strain, R9, was isolated from the South Atlantic sediment sample and identified as Accepted: November 13, 2014 Aspergillus fumigatus. An antifungal protein, AfAFPR9, was purified from the culture supernatant of Aspergillus fumigatus R9. AfAFPR9 was identified to be restrictocin, which is a member of the ribosome-inactivating proteins (RIPs), by MALDI-TOF-TOF-MS. AfAFPR9 First published online displayed antifungal activity against plant pathogenic Fusarium oxysporum, Alternaria longipes, November 14, 2014 Colletotrichum gloeosporioides, Paecilomyces variotii, and Trichoderma viride at minimum *Corresponding author inhibitory concentrations of 0.6, 0.6, 1.2, 1.2, and 2.4 µg/disc, respectively. Moreover, AfAFPR9 Phone: +86-592-2195297; exhibited a certain extent of thermostability, and metal ion and denaturant tolerance. The Fax: +86-592-2085376; iodoacetamide assay showed that the disulfide bridge in AfAFP was indispensable for its E-mail: [email protected] R9 antifungal action. The cDNA encoding for AfAFPR9 was cloned from A. fumigatus R9 by RT- † These authors contributed PCR and heterologously expressed in E. coli. The recombinant AfAFP protein exhibited equally to this work. R9 obvious antifungal activity against C. gloeosporioides, T. viride, and A. longipes. These results reveal the antifungal properties of a RIP member (AfAFPR9) from marine-derived Aspergillus fumigatus and indicated its potential application in controlling plant pathogenic fungi.
    [Show full text]
  • XXI Fungal Genetics Conference Abstracts
    Fungal Genetics Reports Volume 48 Article 17 XXI Fungal Genetics Conference Abstracts Fungal Genetics Conference Follow this and additional works at: https://newprairiepress.org/fgr This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. Recommended Citation Fungal Genetics Conference. (2001) "XXI Fungal Genetics Conference Abstracts," Fungal Genetics Reports: Vol. 48, Article 17. https://doi.org/10.4148/1941-4765.1182 This Supplementary Material is brought to you for free and open access by New Prairie Press. It has been accepted for inclusion in Fungal Genetics Reports by an authorized administrator of New Prairie Press. For more information, please contact [email protected]. XXI Fungal Genetics Conference Abstracts Abstract XXI Fungal Genetics Conference Abstracts This supplementary material is available in Fungal Genetics Reports: https://newprairiepress.org/fgr/vol48/iss1/17 : XXI Fungal Genetics Conference Abstracts XXI Fungal Genetics Conference Abstracts Plenary sessions Cell Biology (1-87) Population and Evolutionary Biology (88-124) Genomics and Proteomics (125-179) Industrial Biology and Biotechnology (180-214) Host-Parasite Interactions (215-295) Gene Regulation (296-385) Developmental Biology (386-457) Biochemistry and Secondary Metabolism(458-492) Unclassified(493-502) Index to Abstracts Abstracts may be cited as "Fungal Genetics Newsletter 48S:abstract number" Plenary Abstracts COMPARATIVE AND FUNCTIONAL GENOMICS FUNGAL-HOST INTERACTIONS CELL BIOLOGY GENOME STRUCTURE AND MAINTENANCE COMPARATIVE AND FUNCTIONAL GENOMICS Genome reconstruction and gene expression for the rice blast fungus, Magnaporthe grisea. Ralph A. Dean. Fungal Genomics Laboratory, NC State University, Raleigh NC 27695 Rice blast disease, caused by Magnaporthe grisea, is one of the most devastating threats to food security worldwide.
    [Show full text]
  • Aspergillus Bibliography
    Fungal Genetics Reports Volume 50 Article 16 Aspergillus Bibliography John Clutterbuck University of Glasgow Follow this and additional works at: https://newprairiepress.org/fgr This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. Recommended Citation Clutterbuck, J. (2003) "Aspergillus Bibliography," Fungal Genetics Reports: Vol. 50, Article 16. https://doi.org/10.4148/1941-4765.1162 This Bibliography is brought to you for free and open access by New Prairie Press. It has been accepted for inclusion in Fungal Genetics Reports by an authorized administrator of New Prairie Press. For more information, please contact [email protected]. Aspergillus Bibliography Abstract This bibliography attempts to cover genetical and biochemical publications on Aspergillus nidulans and also includes selected references to related species and topics. Entries have been checked as far as possible, but please tell me of any errors and omissions. Authors are kindly requested to send a copy of each article to the FGSC for its reprint collection. This bibliography is available in Fungal Genetics Reports: https://newprairiepress.org/fgr/vol50/iss1/16 Clutterbuck: Aspergillus Bibliography Aspergillus Bibliography This bibliography attempts to cover genetical and biochemical publications on Aspergillus nidulans and also includes selected references to related species and topics. Entries have been checked as far as possible, but please tell me of any errors and omissions. Authors are kindly requested to send a copy of each article to the FGSC for its reprint collection. John Clutterbuck. Institute of Biomedical and Life Sciences, Anderson College,University of Glasgow, Glasgow G11 6NU, Scotland, UK. Email: [email protected] The FGN 50 Aspergillus Bibliography Keyword list The FGN 50 Aspergillus Bibliography Author list 1.
    [Show full text]
  • Taxonomic Revision of Aspergillus Section Clavati Based on Molecular, Morphological and Physiological Data
    available online at www.studiesinmycology.org STUDIE S IN MYCOLOGY 59: 89–106. 2007. doi:10.3114/sim.2007.59.11 Taxonomic revision of Aspergillus section Clavati based on molecular, morphological and physiological data J. Varga1,3, M. Due2, J.C. Frisvad2 and R.A. Samson1 1CBS Fungal Biodiversity Centre, Uppsalalaan 8, NL-3584 CT Utrecht, The Netherlands; 2BioCentrum-DTU, Building 221, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark; 3Department of Microbiology, Faculty of Science and Informatics, University of Szeged, H-6701 Szeged, P.O. Box 533, Hungary *Correspondence: János Varga, [email protected] Abstract: Aspergillus section Clavati has been revised using morphology, secondary metabolites, physiological characters and DNA sequences. Phylogenetic analysis of β-tubulin, ITS and calmodulin sequence data indicated that Aspergillus section Clavati includes 6 species, A. clavatus (synonyms: A. apicalis, A. pallidus), A. giganteus, A. rhizopodus, A. longivesica, Neocarpenteles acanthosporus and A. clavatonanicus. Neocarpenteles acanthosporus is the only known teleomorph of this section. The sister genera to Neocarpenteles are Neosartorya and Dichotomomyces based on sequence data. Species in Neosartorya and Neocarpenteles have anamorphs with green conidia and share the production of tryptoquivalins, while Dichotomomyces was found to be able to produce gliotoxin, which is also produced by some Neosartorya species, and tryptoquivalines and tryptoquivalones produced by members of both section Clavati and Fumigati. All species in section Clavati are alkalitolerant and acidotolerant and they all have clavate conidial heads. Many species are coprophilic and produce the effective antibiotic patulin. Members of section Clavati also produce antafumicin, tryptoquivalines, cytochalasins, sarcins, dehydrocarolic acid and kotanins (orlandin, desmethylkotanin and kotanin) in species specific combinations.
    [Show full text]
  • Pathogen-Induced Production of the Antifungal AFP Protein from Aspergillus Giganteus Confers Resistance to the Blast Fungus Magnaporthe Grisea in Transgenic Rice
    MPMI Vol. 18, No. 9, 2005, pp. 960–972. DOI: 10.1094/ MPMI -18-0960. © 2005 The American Phytopathological Society e-Xtra* Pathogen-Induced Production of the Antifungal AFP Protein from Aspergillus giganteus Confers Resistance to the Blast Fungus Magnaporthe grisea in Transgenic Rice Ana Beatriz Moreno,1 Gisela Peñas,2 Mar Rufat,1 Juan Manuel Bravo,1 Montserrat Estopà,2 Joaquima Messeguer,2 and Blanca San Segundo1 Consorcio Laboratorio CSIC-IRTA de Genética Molecular Vegetal: 1Departamento de Genética Molecular, Instituto de Biología Molecular de Barcelona, CSIC. Jordi Girona 18, 08034 Barcelona, Spain; 2Departamento de Genética Vegetal, IRTA Centro de Cabrils. Carretera de Cabrils s/n, Cabrils 08348, Barcelona, Spain Submitted 23 February 2005. Accepted 24 April 2005. Rice blast, caused by Magnaporthe grisea, is the most im- widespread distribution and destructiveness (Ou 1985). Breed- portant fungal disease of cultivated rice worldwide. We ing of durable resistance to this fungus is a difficult problem have developed a strategy for creating disease resistance to not only because of the high degree of pathogenic variability M. grisea whereby pathogen-induced expression of the afp of M. grisea but also because of the large number of fungal (antifungal protein) gene from Aspergillus giganteus occurs races encountered in the field population. Conventional control in transgenic rice plants. Here, we evaluated the activity of of rice blast depends on the use of chemically synthesized fun- the promoters from three maize pathogenesis-related (PR) gicides. However, the repeated use of such chemicals has several genes, ZmPR4, mpi, and PRms, in transgenic rice. Chimeric drawbacks, such as their lack of specificity, increased incidence gene fusions were prepared between the maize promoters of development of resistance upon prolonged application, and and the β-glucuronidase reporter gene (gus A).
    [Show full text]
  • Species-Specific Differences in the Susceptibility of Fungi Towards the Antifungal Protein AFP Depend on C3 Saturation of Glycos
    bioRxiv preprint doi: https://doi.org/10.1101/698456; this version posted July 11, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 1 Species-specific differences in the susceptibility of fungi 2 towards the antifungal protein AFP depend on C3 saturation of 3 glycosylceramides 4 5 Norman Paegea, Dirk Warneckeb, Simone Zäunerb, Silke Hagena, Ana 6 Rodriguesa, Birgit Baumanna, Melanie Thiessb, Sascha Junga# and Vera 7 Meyera# 8 9 aDepartment of Applied and Molecular Microbiology, Institute of Biotechnology, 10 Technische Universität Berlin, Berlin, Germany 11 bInstitute of Plant Science and Microbiology, University of Hamburg, Hamburg, 12 Germany 13 14 Running Head: AFP susceptibility depends on glycosylceramides 15 #Address correspondence to Sascha Jung, [email protected] and Vera 16 Meyer, [email protected] 17 18 Word counts: Abstract: 217, Importance: 81, Text: 5365 (Intro: 1221, MM: 19 1650, Results: 1608, Discussion: 886) 20 21 1 bioRxiv preprint doi: https://doi.org/10.1101/698456; this version posted July 11, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 22 23 24 Abstract 25 AFP is an antimicrobial peptide (AMP) produced by the filamentous fungus 26 Aspergillus giganteus and a very potent inhibitor of fungal growth without 27 affecting the viability of bacteria, plant or mammalian cells. It targets chitin 28 synthesis and causes plasma membrane permeabilization in many human and 29 plant pathogenic fungi, but its exact mode of action is not known.
    [Show full text]