DOCSLIB.ORG

Applications of Chemometrics in Studying the Influences of Genetic

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Applications of Chemometrics in Studying the Influences of Genetic APPLICATIONS OF CHEMOMETRICS IN STUDYING THE INFLUENCES OF GENETIC VARIATION AND PROCESSING ON THE CHEMICAL COMPOSITION OF FOODS A THESIS SUBMITTED TO THE FACULTY OF UNIVERSITY OF MINNESOTA BY WESLEY MOSHER IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE Name of Adviser: Chi Chen August 2020 © Wesley Mosher 2020 ACKNOWLEDGEMENTS As I reflect on the last couple of years, I must first acknowledge an overwhelming sense of gratitude towards my advisor, Dr. Chi Chen. As I have had the opportunity to work under his guidance, his passion for scientific inquiry has been evident each day. The standard of quality he has instilled throughout the time of my studies and this thesis research will serve me well in my future career. This thesis work would not be what it is without his insights, patience, and encouragement. I would also like to thank my lab members. It is the environment they create each and every day that has made my experience at the University of Minnesota forever memorable. Coming to the lab each day feels like spending time with family, which is a testament to the quality of friendships that have been so easily built among each of us. To Qingqing Mao, Yuyin Zhou, Yiwei Ma, Yue Guo, and Jieyao Yuan–thank you. Also, to Dana Yao–thank you for always being willing to share your technical expertise and experience. It is your support that makes the Chen Lab an incredibly unique experience. Finally, to my parents–thank you for the support of your company, encouragement, and more during this time. I would not be where I am without your continual support. i ABSTRACT Genetic variation is an essential internal factor while processing is a common external factor that determines the chemical composition of food. Characterization of chemical changes from genetic variation and processing is a challenging but also important task in monitoring the organoleptic, nutritional, and safety properties of food. This thesis study examined the efficacy of liquid chromatography-mass spectrometry (LC-MS)-based chemometric analysis in defining the chemical features associated with genetic variation and processing through two case studies on wild rice and mesquite flour, respectively. Wild rice (Zizania spp.), an ancient grain revered by Native Americans, has a more desirable nutritional profile in comparison with Asian rice. However, the differences between the lipidome of wild rice and Asian rice has not been well examined. In this study, the LC-MS-based untargeted profiling was performed on the lipid extracts from wild rice, white rice, and brown rice. Triacylglycerols (TAG) rich in essential fatty acids α-linolenic acid (ALA) and linoleic acid (LA) and the steradienes from phytosterol dehydration emerged as the primary features that separate wild rice from Asian rice. The phytosterol content of wild rice was further analyzed through the quantification of γ-oryzanol and stigmasterol, illustrating the enrichment of these phytosterols in wild rice. The presence of steradienes in wild rice is attributed to its unique processing after harvesting. Chemical influences of processing were investigated by conducting the LC-MS-based chemometric analysis of the mesquite flour (Prosopis spp.) treated with intense-pulsed light (IPL), a novel non-thermal disinfection processing that is compatible with powdered food. Targeted analysis of acetic acid and propionic acid, two flavor compounds, showed that the influences of IPL on the concentration of ii these two short-chain fatty acids were comparable to the effects of γ-irradiation, another non-thermal disinfection method. The untargeted analysis showed that IPL increased 9- hydroyoctadecadienoic acid (HODE), a lipid oxidation product, as well as α- ketoisovaleric acid, a degradation product of amino acids. Overall, LC-MS-based chemometric analysis proved to be a powerful tool in assessing the alterations in food due to genetic variation and processing. iii TABLE OF CONTENTS ACKNOWLEDGEMENTS ................................................................................................. i ABSTRACT ........................................................................................................................ ii TABLE OF CONTENTS ................................................................................................... iv LIST OF TABLES ............................................................................................................. vi LIST OF FIGURES .......................................................................................................... vii CHAPTER 1: LITERATURE REVIEW ............................................................................ 1 1.1 INFLUENCES OF GENETIC VARIATION ON THE CHEMICAL COMPOSITION OF FOODS: CASE STUDY ON WILD RICE .................................. 2 1.1.1 Botanic classification of wild rice................................................................. 2 1.1.2 History and cultural implications of wild rice research ................................ 3 1.1.3 Chemical composition of wild rice ............................................................... 4 1.2 INFLUENCES OF PROCESSING ON CHEMICAL COMPOSITION OF FOODS: CASE STUDY ON INTENSE PULSED LIGHT ......................................... 11 1.2.1 IPL and its disinfection application on foodborne microbes ...................... 11 1.2.2 Effects of non-ionizing treatments on chemicals in foods .......................... 14 1.3 PRINCIPLES AND PRACTICE OF CHEMOMETRICS ..................................... 15 1.3.1 Introduction of chemometrics platform ........................................................... 15 1.3.2 Applications of chemometrics in food and nutritional analysis....................... 16 CHAPTER 2: CHEMOMETRIC ANALYSIS OF WILD RICE ..................................... 28 2.1 INTRODUCTION .................................................................................................. 29 2.2 METHODS AND MATERIALS ............................................................................ 30 2.2.1 Rice samples .................................................................................................... 30 2.2.2 Chemicals ......................................................................................................... 31 2.2.3 Sample preparation .......................................................................................... 31 2.2.4 LC-MS analysis ............................................................................................... 31 2.2.5 Multivariate data analysis ................................................................................ 32 2.3 RESULTS AND DISCUSSION ............................................................................. 33 2.3.1 Triacylglycerols in rice. ................................................................................... 33 2.3.2 Phytosterols in wild rice................................................................................... 36 2.4 CONCLUSION ....................................................................................................... 39 iv CHAPTER 3: CHEMOMETRIC ANALSYIS OF INTENSE PULSED LIGHT- ELICITED CHEMICAL CHANGES IN MESQUITE FLOUR ...................................... 47 3.1 INTRODUCTION .................................................................................................. 48 3.2 MATERIALS AND METHODS ............................................................................ 50 3.1.1 Samples and chemicals .................................................................................... 50 3.1.2 Sample preparation .......................................................................................... 51 3.1.3 LC-MS Analysis .............................................................................................. 52 3.1.4 Multivariate modeling and data visualization .................................................. 52 3.3 RESULTS AND DISCUSSION ............................................................................. 53 3.2.1 Targeted analysis ............................................................................................. 53 3.2.2 Untargeted chemometric profiling ................................................................... 53 3.4 CONCLUSION ....................................................................................................... 55 REFERENCES ................................................................................................................. 59 v LIST OF TABLES Table 1.1. Macronutrient composition of wild rice, white rice, and brown rice...............24 Table 1.2. Micronutrient composition of wild rice, white rice, and brown rice...............25 Table 2.1. Composition of identified TAGs in rice...........................................................40 Table 2.2. Identities of detected steradienes in wild rice..................................................41 Table 3.1. Chemical markers separating IPL and γ-irradiation treatments from control................................................................................................................................55 vi LIST OF FIGURES Figure 1.1. Phylogenetic classification of selected staple gains.......................................26 Figure 1.2. LC-MS-based chemometric analysis workflow.............................................27 Figure 2.1. Representative two-dimensional chromatograms of white (A), brown (B), and wild rice (C). Representative fragmentation pattern of triglycerides
Load more

Recommended publications
  • [Zizania Latifolia (Griseb.) Turcz. Ex Stapf] (Poaceae) in Manipur
    Indian Journal of Traditional Knowledge Vol.11 (1), January 2012, pp. 66-69 The ethnobotany and nutritional values of wild rice [Zizania latifolia (Griseb.) Turcz. ex Stapf] (Poaceae) in Manipur Alka Jain1, H Birkumar Singh1* & PR Bhattacharyya2 1North-East Institute of Science & Technology (CSIR), Substation, Lamphelpat 795 004, Imphal, Manipur, India 2North-East Institute of Science & Technology (CSIR), Jorhat 785 006, Assam, India *E-mail: [email protected] Received 30.07.10; revised 29.01.11 Wild rice [Zizania latifolia (Griseb.) Turcz ex stapf.)], locally called ‘Kambong’ or ‘Ishing-kambong’ (in Manipuri) is an aquatic/wetland plant of Manipur and is used for various purposes by the ethnic people ranging from food to fodder, thatch roofing, firewood substitute, house wall plastering material, etc. The infected gall forming culm (infected with the fungus Ustilago esculenta P. Henn.) is highly eaten by the local people of Manipur and regarded as delicacy and fetching Rs 28-55 per kg and is a source of livelihood for a section of rural populace. The galls are nutritious with protein (8.13%), carbohydrate (13.82%) and with other micro and macro-nutrients. The edible part of the plant is also having good antioxidant properties like total phenolics (84.1 mg GAE/g dry weight of plant), total flavonoides (7mg QE/g dry wt of plant) and antiradical power value of 10.4. Presence of toxic arsenic (0.09 ppm) in wild rice is much below the permissible limit and hence quite safe for consumption. The tender shoot of this plant is fondly eaten by Cervus eldi eldi McClelland, a critically endangered deer species of the world and also the ‘state animal’ of Manipur.
    [Show full text]
  • ARF) in Zizania Latifolia and Its Role in Swelling Stem Formation After Ustilago Esculenta Infection
    Genome-Wide Identication Analysis of The Auxin Response Factor Gene Family (ARF) in Zizania Latifolia and its Role in Swelling Stem Formation After Ustilago Esculenta Infection Jie Li Anhui Agricultural University Jinfeng Hou Anhui Agricultural University Zhiyuan Lu Anhui Agricultural University Yang Yang Anhui Agricultural University Hao Wang Anhui Agricultural University Ying Wu Anhui Agricultural University Lingyun Yuan Anhui Agricultural University Guohu Chen Anhui Agricultural University Chenggang Wang Anhui Agricultural University Defang Gan Anhui Agricultural University Shidong Zhu ( [email protected] ) Anhui Agricultural University https://orcid.org/0000-0001-6046-9060 Research article Keywords: ZlARF, Z. latifolia, Phylogenetic analysis, auxin-activated signalling pathway, Swelling stem formation, Transcriptomic analysis, U. esculenta infection Posted Date: January 22nd, 2021 DOI: https://doi.org/10.21203/rs.3.rs-151191/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Page 1/18 Abstract Background: Auxin signalling plays a crucial role in plant growth and development. Although the auxin response factor (ARF) gene family has been studied in some plant species, its structural features, molecular evolution, and expression prole in Zizania latifolia (Z. latifolia) are still not clear. Results: Our study identied 33 putative YSL genes from the whole Z. latifolia genome. Furthermore, a comprehensive overview of the ZlARFs was undertaken, including phylogenetic relationship, gene structures, conserved domains, synteny, Ka/Ks, motifs, and subcellular locations of the gene product. Synteny analyses and the calculation of Ka/Ks values suggested that all 57 orthologous/paralogous gene pairs between Z. latifolia and Z. latifolia, Z. latifolia and Oryza sativa have experienced strong purifying selection.
    [Show full text]
  • Dictionary of Cultivated Plants and Their Regions of Diversity Second Edition Revised Of: A.C
    Dictionary of cultivated plants and their regions of diversity Second edition revised of: A.C. Zeven and P.M. Zhukovsky, 1975, Dictionary of cultivated plants and their centres of diversity 'N -'\:K 1~ Li Dictionary of cultivated plants and their regions of diversity Excluding most ornamentals, forest trees and lower plants A.C. Zeven andJ.M.J, de Wet K pudoc Centre for Agricultural Publishing and Documentation Wageningen - 1982 ~T—^/-/- /+<>?- •/ CIP-GEGEVENS Zeven, A.C. Dictionary ofcultivate d plants andthei rregion so f diversity: excluding mostornamentals ,fores t treesan d lowerplant s/ A.C .Zeve n andJ.M.J ,d eWet .- Wageninge n : Pudoc. -11 1 Herz,uitg . van:Dictionar y of cultivatedplant s andthei r centreso fdiversit y /A.C .Zeve n andP.M . Zhukovsky, 1975.- Me t index,lit .opg . ISBN 90-220-0785-5 SISO63 2UD C63 3 Trefw.:plantenteelt . ISBN 90-220-0785-5 ©Centre forAgricultura l Publishing and Documentation, Wageningen,1982 . Nopar t of thisboo k mayb e reproduced andpublishe d in any form,b y print, photoprint,microfil m or any othermean swithou t written permission from thepublisher . Contents Preface 7 History of thewor k 8 Origins of agriculture anddomesticatio n ofplant s Cradles of agriculture and regions of diversity 21 1 Chinese-Japanese Region 32 2 Indochinese-IndonesianRegio n 48 3 Australian Region 65 4 Hindustani Region 70 5 Central AsianRegio n 81 6 NearEaster n Region 87 7 Mediterranean Region 103 8 African Region 121 9 European-Siberian Region 148 10 South American Region 164 11 CentralAmerica n andMexica n Region 185 12 NorthAmerica n Region 199 Specieswithou t an identified region 207 References 209 Indexo fbotanica l names 228 Preface The aimo f thiswor k ist ogiv e thereade r quick reference toth e regionso f diversity ofcultivate d plants.Fo r important crops,region so fdiversit y of related wild species areals opresented .Wil d species areofte nusefu l sources of genes to improve thevalu eo fcrops .
    [Show full text]
  • A Global Perspective on the Origins of Agriculture: the Importance of Unconscious Selection
    A global perspective on the origins of agriculture: the importance of unconscious selection Thomas Kluyver Department of Animal and Plant Sciences A thesis submitted for the degree of Doctor of Philosophy July 2013 1 Acknowledgements My primary supervisor, Colin Osborne, has provided advice, encouragement and inspiration throughout my PhD. My supervisors in the Department of Archaeology, Glynis Jones and Mike Charles, have patiently helped me to get to grips with a field which I had never studied before this project. Mark Rees’ advice about statistics has also been invaluable. I am grateful to Irene Johnson, for her eminently practical help with growing all kinds of plants, and to Emily Mockford and Chris Bennett, for painstakingly dissecting beet seed capsules to weigh individual seeds. Katherine Haynes and Rebecca Crabtree weighed seed of modern garden vegetables for chapter 3, and that chapter also could not have been written without people and organisations around the world who shared their data with me, including Benoît Pujol (Laboratoire Évolution et Diversité Biologique, France), the Botanical Information Section at RBG Kew, the USDA National Genetic Resources Program, the International Potato Centre (CIP) in Peru, and EMBRAPA in Brazil. Over the last few years, I have enjoyed a warm, friendly and intellectually stimulating environment in Sheffield. It has been a pleasure to work with the people in Colin Osborne’s lab group, as well as the many others who I have got to know. My PhD research was funded by a university studentship from the University of Sheffield, for which I am very thankful. Last but not least, my thanks to my girlfriend and my family, for their support both during my PhD and in the years of education which prepared me to undertake it.
    [Show full text]
  • Partial Purification, Identification, and Quantitation of Antioxidants From
    molecules Article Partial Purification, Identification, and Quantitation of Antioxidants from Wild Rice (Zizania latifolia) Mei-Jun Chu 1, Xin-Min Liu 1, Ning Yan 1, Feng-Zhong Wang 2, Yong-Mei Du 1,* and Zhong-Feng Zhang 1,* 1 Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, China; [email protected] (M.-J.C.); [email protected] (X.-M.L.); [email protected] (N.Y.) 2 Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China; [email protected] * Correspondence: [email protected] (Y.-M.D.); [email protected] (Z.-F.Z.); Tel.: +86-532-88702239 (Z.-F.Z.) Received: 15 September 2018; Accepted: 23 October 2018; Published: 26 October 2018 Abstract: To provide further insights into the potential health-promoting antioxidants from wild rice (Zizania latifolia), which is an abundant but underutilized whole grain resource in East Asia, a partial purification based on D101 macroporous resin was carried out for the purification and enrichment of the antioxidants from the bioactive ethanol extracts of wild rice. On that basis, 34 phenolic compounds in the antioxidant fractions were identified by a high-performance liquid chromatography-linear ion trap quadrupole-Orbitrap-mass spectrometry (HPLC-LTQ-Orbitrap-MSn). The results suggested that phenolic acids could be enriched in the 10% ethanol-eluted fraction whereas flavonoids (including procyanidins and flavonoid glycosides) could be enriched in 20–30% ethanol-eluted fractions. A quantitative analysis determined by the multiple reaction monitoring mode of the ultra-performance liquid chromatography-triple quadrupole-tandem mass spectrometry (UPLC-QqQ-MS/MS) revealed a high content of procyanidins in wild rice.
    [Show full text]
  • Manchurian Wild Rice ( Zizania Latifolia)
    Seventeenth Australasian Weeds Conference Manchurian wild rice (Zizania latifolia) biomass allocation and implications for control Paul D. Champion and Deborah E. Hofstra National Institute of Water and Atmospheric Research (NIWA), PO Box 11-115, Hamilton 3251, New Zealand Corresponding author: [email protected] Summary Manchurian wild rice (Zizania latifolia) the South Head of the Kaipara, Helensville, Laingholm is a giant invasive grass that forms dense monospecific and Mangere), Waikato (Waihou, Piako and Awaiti stands of vegetation on the margins of water bodies, Rivers on the Hauraki Plains and Horohoro and Lake also invading flood-prone pasture and cropping land. Te Koutu near Cambridge) and Wellington (Waikanae) A national eradication programme has been initiated (Champion and Hofstra 2008). for this species. MAF Biosecurity New Zealand has instigated a Biomass for component parts of the plant was National Interest Pest Response (NIPR) Programme on measured in pot and field trials. In the field Man- this species, with the initial aim of eradicating all sites churian wild rice produced a total biomass of 109.9 outside of the Northern Wairoa River, with national tonnes dry weight haí1, with ~80% of this biomass eradication the long-term goal (Anon. 2009). below ground (mostly rhizomes). Of the shoot bio- Manchurian wild rice is notoriously difficult to mass, 41.4% is dead and in pot trials this percentage control using herbicides, with glyphosate, or a com- was higher. The dead biomass was predominantly bination of dalapon and amitrole, the most commonly comprised of shoot bases with large amounts of aeren- used to date (P.
    [Show full text]
  • Literature Review of Potential Health Benefits of Wild Rice and Wild Rice
    Potential Health Benefits of Wild Rice and Wild Rice Products: Literature Review July 2012 By: Daniel D. Gallaher, Ph.D. (Principal Contact) Professor Department of Food Science and Nutrition University of Minnesota 612.624.0746, [email protected] 1334 Eckles Ave. St. Paul, MN 55108 Mirko Bunzel, Ph.D. Professor Chair of the Department of Food Chemistry and Phytochemistry Karlsruhe Institute of Technology – KIT Faculty of Chemistry and Biosciences, Institute of Applied Biosciences +49 721 608 42936, [email protected] TABLE OF CONTENTS INTRODUCTION ................................................................................................................................................................... 4 PHYTOCHEMICALS IN WILD RICE INCLUDING A LIST OF PHYTOCHEMICALS WITH POTENTIAL HEALTH BENEFITS ................. 4 DEFINITION OF PHYTOCHEMICALS .................................................................................................................................................... 4 PRIMARY METABOLITES FROM WILD RICE AFFECTING THE NUTRITIONAL QUALITY OF WILD RICE .................................................................... 5 Lipids .................................................................................................................................................................................. 5 Lipid content ..................................................................................................................................................................................... 5 Fatty
    [Show full text]
  • Ultrastructure and Phylogeny of Ustilago Coicis*
    336 Zhang et al. / J Zhejiang Univ-Sci B (Biomed & Biotechnol) 2013 14(4):336-345 Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology) ISSN 1673-1581 (Print); ISSN 1862-1783 (Online) www.zju.edu.cn/jzus; www.springerlink.com E-mail: [email protected] Ultrastructure and phylogeny of Ustilago coicis* Jing-ze ZHANG†1, Pei-gang GUAN1, Gang TAO2,3, Mohammad Reza OJAGHIAN1, Kevin David HYDE4 (1Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China) (2Guizhou Institute of Plant Protection, Guiyang 550006, China) (3Key Laboratory of Systematic Mycology and Lichenology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China) (4Institutue of Excellence in Fungal Research, School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand) †E-mail: [email protected] Received Sept. 6, 2012; Revision accepted Nov. 9, 2012; Crosschecked Mar. 15, 2013 Abstract: Ustilago coicis causes serious smut on Coix lacryma-jobi in Dayang Town, Jinyun County, Zhejiang Province of China. In this paper, ultrastructural assessments on fungus-host interactions and teliospore development are presented, and molecular phylogenetic analyses have been done to elucidate the phylogenetic placement of the taxon. Hyphal growth within infected tissues was both intracellular and intercellular and on the surface of fungus-host interaction, and the fungal cell wall and the invaginated host plasma membrane were separated by a sheath com- prising two distinct layers between the fungal cell wall and the invaginated host plasma membrane. Ornamentation development of teliospore walls was unique as they appeared to be originated from the exosporium. In addition, in- ternal transcribed spacer (ITS) and large subunit (LSU) sequence data showed that U.
    [Show full text]
  • On Insulin Resistance in Rats Fed with a High-Fat/Cholesterol Diet
    Nutrients 2013, 5, 552-564; doi:10.3390/nu5020552 OPEN ACCESS nutrients ISSN 2072-6643 www.mdpi.com/journal/nutrients Article Effects of Dietary Carbohydrate Replaced with Wild Rice (Zizania latifolia (Griseb) Turcz) on Insulin Resistance in Rats Fed with a High-Fat/Cholesterol Diet Shufen Han 1,2, Hong Zhang 1, Liqiang Qin 2 and Chengkai Zhai 1,* 1 Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, 87 Dingjiaqiao Road, Nanjing 210009, China; E-Mails: [email protected] (S.H.); [email protected] (H.Z.) 2 Department of Nutrition and Food Hygiene, School of Public Health, Medical College of Soochow University, 199 Renai Road, Suzhou 215123, China; E-Mail: [email protected] * Author to whom correspondence should be addressed; E-Mail: [email protected]; Tel./Fax: +86-258-327-2560. Received: 24 December 2012; in revised form: 25 January 2013 / Accepted: 6 February 2013 / Published: 15 February 2013 Abstract: Wild rice (WR) is a very nutritious grain that has been used to treat diabetes in Chinese medicinal practice. City diet (CD) is based on the diet consumed by Asian area residents in modern society, which is rich in saturated fats, cholesterol and carbohydrates. The present study was aimed at evaluating the effects of replacing white rice and processed wheat starch of CD with WR as the chief source of dietary carbohydrates on insulin resistance in rats fed with a high-fat/cholesterol diet. Except the rats of the low-fat (LF) diet group, the rats of the other three groups, including to high-fat/cholesterol (HFC) diet, CD and WR diet, were fed with high-fat/cholesterol diets for eight weeks.
    [Show full text]
  • Optimizing Ultrasound-Assisted Deep Eutectic Solvent Extraction of Bioactive Compounds from Chinese Wild Rice
    molecules Article Optimizing Ultrasound-Assisted Deep Eutectic Solvent Extraction of Bioactive Compounds from Chinese Wild Rice 1,2, 1, 1 1,2 1, 1,3, Jia Zeng y, Yuqing Dou y, Ning Yan , Na Li , Huaibao Zhang * and Jia-Neng Tan * 1 Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China 2 Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China 3 Key Laboratory of Oilseeds Processing, Ministry of Agriculture, Wuhan 430062, China * Correspondence: [email protected] (H.Z.); [email protected] (J.-N.T.); Tel.: +86-532-8870-2052 (H.Z.); Fax: +86-532-8870-2239 (H.Z.) These authors contributed equally to this work. y Received: 1 July 2019; Accepted: 24 July 2019; Published: 26 July 2019 Abstract: In this study, deep eutectic solvents (DESs) were used for the ultrasound-assisted extraction (UAE) of valuable bioactive compounds from Chinese wild rice (Zizania spp.). To this end, 7 different choline chloride (CC)-based DESs were tested as green extraction solvents. Choline chloride/1,4- butanediol (DES-2) exhibited the best extraction efficiency in terms of parameters such as the total flavonoid content (TFC), total phenolic content (TPC), and free radical scavenging capacity + (DPPH• and ABTS• ). Subsequently, the UAE procedure using 76.6% DES-2 was also optimized: An extraction temperature of 51.2 ◦C and a solid–liquid ratio of 37.0 mg/mL were considered optimal by a Box–Behnken experiment. The optimized extraction procedure proved efficient for the extraction of 9 phenolic and 3 flavonoid compounds from Chinese wild rice as determined by quantification based on ultra-performance liquid chromatography–triple quadrupole tandem mass spectrometry (UPLC-QqQ-MS).
    [Show full text]
  • Chilo Suppressalis
    Chilo suppressalis Scientific name Chilo suppressalis Walker Synonyms Jartheza simplex, Chilo oryzae, Chilo simplex, and Crambus suppressalis Common names Asiatic rice borer, striped rice stem borer, striped rice stalk borer, rice stem borer, rice chilo, purple-lined borer, rice borer, sugarcane moth borer, pale-headed striped borer, and rice stalk borer. Type of pest Moth Taxonomic position Class: Insecta, Order: Lepidoptera, Family: Crambidae Reason for Inclusion in Manual CAPS Target: AHP Prioritized Pest List – 2009 & 2010 Figure 1. Chilo suppresalis egg masses. Image Pest Description courtesy of International Eggs: Eggs (Fig. 1) are fish scale-like, about 0.9 x 0.5 Rice Research Institute mm, turning from translucent-white to dark-yellow as Archive. www.bugwood.org they mature. They are laid in flat, overlapping rows containing up to 70 eggs. Eggs of other Chilo spp. are quite similar and cannot be easily distinguished (UDSA, 1988). Larvae: First-instar larvae are grayish-white with a black head capsule and are about 1.5 mm long (CABI, 2007). The head capsule of later instars becomes lighter in color, changing to brown. Last instar larvae (Fig. 2) are 20-26 mm long, taper slightly toward each end, and are dirty- white, with five longitudinal purple to brown stripes running down the dorsal surface of the body (Hill, 1983). Pupae: Pupae are reddish-brown, 11-13 mm Figure 2. Chilo suppresalis larva. long, 2.5 mm wide (Hill, 1983) and have two Image courtesy of Probodelt, SL. ribbed crests on the pronotal margins and two short horns on the head. The cremaster (the terminal spine of the abdomen) bears several small spines (Hattori and Siwi, 1986).
    [Show full text]
  • Wild Rice Gathering Wild Rice: Ancient Legacies Online Exhibits
    National Agricultural Library United States Department of Agriculture Wild Rice Gathering Wild Rice: Ancient Legacies Online Exhibits “Gathering Wild Rice: Digital Collections for the Classroom.” Accessed July 21, 2020. https://dcc.newberry.org/items/gathering_wild_rice. Includes plates from Seth Eastman. From The American Aboriginal Portfolio, by Mary H. Eastman. c. 1853. Three Native American women (Chippewa?) in a birch bark canoe gather seeds from wild rice. They use paddles to beat out the seeds. Artist) Seth Eastman, American 1808-1875 Digital Public Library of America. “Ancient Legacies.” Accessed July 21, 2020. https://dp.la/exhibitions/history-of-survivance/ancient- legacies/gathering-wild-rice. Gathering wild rice, Detroit Lake, Minnesota “Anishinaabe people have been harvesting manoomin (wild rice) since time 1 | Page National Agricultural Library United States Department of Agriculture immemorial. They relate that manoomin was one of the reasons they came to Minnesota; …. This will be the land where food grows upon the waters.” Ricing continues to this day, though this staple food of woodland Minnesota ” National Gallery of Art. “Gathering Wild Rice – Winnebago”, 1861/1869. Accessed August 6, 2020. https://www.nga.gov/collection/art-object-page.50446.html George Catlin (artist) American 1796-1872 Wild Rice – Selected Bibliography Barton, Barbara J. Manoomin: The Story of Wild Rice in Michigan. East Lansing: Michigan State University Press, 2018. “Cawildrice.Com - Cawildrice Resources and Information.” Accessed July 18, 2020. http://www.cawildrice.com/. Huff, Ned L. Zizania; Wild Rice. Accessed July 18, 2020. http://umedia.lib.umn.edu/item/p16022coll174:2387. 2 | Page National Agricultural Library United States Department of Agriculture Internet Archive.
    [Show full text]