DOCSLIB.ORG

An Epitope‐Imprinted Biointerface with Dynamic Bioactivity For

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
An Epitope‐Imprinted Biointerface with Dynamic Bioactivity For An Epitope-Imprinted Biointerface with Dynamic Bioactivity for Modulating Cell-Biomaterial Interactions Pan, G., Shinde, S., Yeung, S. Y., Jakštaitė, M., Li, Q., Wingren, A. G., & Sellergren, B. (2017). An Epitope- Imprinted Biointerface with Dynamic Bioactivity for Modulating Cell-Biomaterial Interactions. Angewandte Chemie International Edition, 56(50), 15959-15963. https://doi.org/10.1002/anie.201708635 Published in: Angewandte Chemie International Edition Document Version: Publisher's PDF, also known as Version of record Queen's University Belfast - Research Portal: Link to publication record in Queen's University Belfast Research Portal Publisher rights © 2020 The Authors. This is an open access article published under a Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited. General rights Copyright for the publications made accessible via the Queen's University Belfast Research Portal is retained by the author(s) and / or other copyright owners and it is a condition of accessing these publications that users recognise and abide by the legal requirements associated with these rights. Take down policy The Research Portal is Queen's institutional repository that provides access to Queen's research output. Every effort has been made to ensure that content in the Research Portal does not infringe any person's rights, or applicable UK laws. If you discover content in the Research Portal that you believe breaches copyright or violates any law, please contact [email protected]. Download date:05. Oct. 2021 Angewandte Communications Chemie International Edition:DOI:10.1002/anie.201708635 Dynamic Biointerfaces Very Important Paper German Edition:DOI:10.1002/ange.201708635 An Epitope-Imprinted Biointerface with DynamicBioactivity for Modulating Cell–Biomaterial Interactions Guoqing Pan,* Sudhirkumar Shinde,Sing YeeYeung,Migle˙ Jaksˇtaite˙,Qianjin Li, Anette Gjçrloff Wingren, and Bçrje Sellergren* Abstract: In this study,anepitope-imprinting strategy was great promise in drug targeting and isolation methods for employed for the dynamic displayofbioactive ligands on therapeutics and diagnostics.[3] amaterial interface.Animprinted surface was initially Current methods to control reversible ligand presentation designed to exhibit specific affinity towardsashort peptide on biomaterial interfaces mainly rely on surface functional- (i.e., the epitope). This surface was subsequently used to ization with reversible linkers (e.g.,noncovalent or reversible anchor an epitope-tagged cell-adhesive peptide ligand (RGD: covalent interactions) to which the bioactive ligand is Arg-Gly-Asp). Owing to reversible epitope-binding affinity, tethered. Forexample,bymeans of host–guest interactions,[4] ligand presentation and thereby cell adhesion could be reversible covalent interactions,[5] molecular assembly,[6] or controlled. As compared to current strategies for the fabrica- other multiple noncovalent interactions,[7] the integrin-tar- tion of dynamic biointerfaces,for example,through reversible geted cell-adhesive peptide RGD (Arg-Gly-Asp)[8] could be covalent or host–guest interactions,such amolecularly tunable dynamically and reversibly immobilized on biointerfaces to dynamic system based on asurface-imprinting process may regulate cell-adhesion behavior. These approaches towards unlock new applications in in situ cell biology,diagnostics,and the simulation of reversible ligand presentation in abiological regenerative medicine. system have greatly promoted the development of dynamic biointerfaces and anew generation of artificial ECM materi- Cellular processes are crucially dependent on dynamic als.Todate,only afew reversible linkage chemistries have receptor–ligand interactions occurring at the interface been exploited. In view of the vast number of natural between the cell membrane and the extracellular matrix receptor–ligand pairs in biology,further effort is warrented. (ECM).[1] Changes in these interactions as aconsequence of Linkages mimicking the exquisite complementarity exhibited ECM remodeling give rise to specific cell-signaling and by natural ligand–receptor pairs are particularly attractive,[9] intracellular cascades.These processes are central to physi- but current strategies in this direction still leave significant ology and pathological processes,such as tissue self-repair room for improvement. and tumorigenesis.[1b,c] As mimics of such dynamic interac- To attain molecularly tunable reversibility in analogy with tions,artificial matrices with the reversible display of natural receptor–ligand interactions we turned to molecular bioactive ligands have attracted much attention. Surfaces imprinting and molecularly imprinted polymers (MIPs).[10] capable of modulating cell–biomaterial interactions are Therecognition sites in MIPs,generally created by atem- commonly exploited for in situ cell-biology experimentation plate-imprinting process,are spatially complementary to the and in tissue engineering.[1c,2] Furthermore,adynamic bio- shape and functionality of the template molecules.[10a–c] Thus, interface with reversibly immobilized ligands has also shown molecular recognition in MIPs occurs by a“lock and key” mechanism similar to natural receptor–ligand interactions.In [*] Prof. Dr.G.Pan, Dr.S.Shinde, S. Y. Yeung, M. Jaksˇtaite˙,Dr. Q. Li, contrast to the scarce number of dynamic covalent or host– Prof. Dr.A.G.Wingren, Prof. Dr.B.Sellergren guest-based linkages,[11] imprinting can be used to tailor Department of Biomedical Sciences reversible affinity towards various ligands,including small Faculty of Health and Society,Malmç University molecules,peptides,and proteins.[12] It has been shown that SE 205 06 Malmç (Sweden) MIPs now can challenge and even exceed the performance of E-mail:[email protected] [email protected] antibodies in affinity related applications both in vitro and in [12a,13] Prof. Dr.G.Pan vivo. To date,however,few attempts have been made to Institute for Advanced Materials use molecular imprinting for the reversible introducation of School of Materials Science and Engineering, Jiangsu University surface bioactivity.[14] Zhenjiang, Jiangsu, 212013 (China) Recently,wedesigned an RGD-peptide-imprintedsub- E-mail:[email protected] strate to assist fast cell-sheet harvesting.[12c] Given that we Supportinginformation and the ORCID identification number(s) for then used bioactive ligands as templates per se in that system, the author(s) of this article can be found under: template binding would produce two counteracting effects: https://doi.org/10.1002/anie.201708635. 1) it would concentrate the bioactive molecules near the 2017 The Authors. Published by Wiley-VCH Verlag GmbH & Co. interface,and 2) tightly bound bioactive molecules would be KGaA. This is an open access article under the terms of the Creative unaccessible for receptor binding. Commons Attribution-NonCommercial-NoDerivs License, which permitsuse and distribution in any medium, provided the original To address the latter problem, we employed in this study [15] work is properly cited, the use is non-commercial and no modifica- an epitope-imprinting strategy for introducing cell-adhe- tions or adaptations are made. sive RGD in afully exposed form. Theprinciple is based on Angew.Chem. Int.Ed. 2017, 56,15959 –15963 2017 The Authors. Published by Wiley-VCH Verlag GmbH &Co. KGaA, Weinheim 15959 Angewandte Communications Chemie the use of ashort peptide (the epitope) as template during the (DDDGGDDDSSSSSRGDS) consisting of the epitope tag imprinting process and subsequently use of this epitope as an (DDDGGDDD) at the N-terminus,ahydrophilic spacer RGD tag for anchoring the RGD sequence to the substrate (SSSSS) in the middle,and an integrin-targeted peptide surface.Inthis design, the epitope peptide could act as (RGDS) at the C-terminal end, was designed for subsequent areversible anchor of RGD peptide,thus leaving the latter peptide rebinding and cell-recognition experiments.Before exposed for interaction with cell-surface integrin receptors. microcontact imprinting,the epitope template was covalently Moreover,the addition of an appropriate amount of epitope immobilized on acover glass at the C-terminal end (see peptide to the system would induce gradual release of the Figure S1 in the Supporting Information). Theimprinting was bound RGD-based peptides through competitive molecular subsequently performed between the cover glass with the exchange;that is,the bioactivity at the material interface can immobilized epitope and amethacrylate-modified quartz be dynamically controlled. substrate (10 mm in diameter). Thepolymerization reaction To obtain high epitope affinity in the resultant MIPs for was photochemically initiated by aUVlamp after dripping stable RGD anchoring,wefocused on charged peptide 3 mLofaphosphate buffer solution (PBS,0.02m,pH7.4) sequences capable of engaging in multiple ionic interactions containing 1m 2-hydroxyethyl acrylamide (hydrophilic with afunctional monomer.Exploitation of the amidine– backbone monomer), 0.01m 4-acrylamidophenyl(amino)- carboxylate interaction seemed particularly worthwhile given methaniminium chloride (benzamidine-bearing monomer; its high stability in competitive solvents and previous success see Figure S2),[13d] and 0.05m methylenebisacrylamide in molecular imprinting,[16] for example,the use of benzami- (cross-linker) onto the cover glass.Bypolymerization
Load more

Recommended publications
  • University of Birmingham the Increasing Dynamic, Functional
    University of Birmingham The increasing dynamic, functional complexity of bio-interface materials Gomes, Bárbara; Simões, Bárbara Sofia; Mendes, Paula DOI: 10.1038/s41570-018-0120 License: None: All rights reserved Document Version Peer reviewed version Citation for published version (Harvard): Gomes, B, Simões, BS & Mendes, P 2018, 'The increasing dynamic, functional complexity of bio-interface materials', Nature Reviews Chemistry, vol. 2, no. 3, 0120. https://doi.org/10.1038/s41570-018-0120 Link to publication on Research at Birmingham portal Publisher Rights Statement: Checked for eligibility 02/11/2018 Published in Nature Reviews Chemistry https://doi.org/10.1038/s41570-018-0120 General rights Unless a licence is specified above, all rights (including copyright and moral rights) in this document are retained by the authors and/or the copyright holders. The express permission of the copyright holder must be obtained for any use of this material other than for purposes permitted by law. •Users may freely distribute the URL that is used to identify this publication. •Users may download and/or print one copy of the publication from the University of Birmingham research portal for the purpose of private study or non-commercial research. •User may use extracts from the document in line with the concept of ‘fair dealing’ under the Copyright, Designs and Patents Act 1988 (?) •Users may not further distribute the material nor use it for the purposes of commercial gain. Where a licence is displayed above, please note the terms and conditions of the licence govern your use of this document. When citing, please reference the published version.
    [Show full text]
  • Nanoparticles and Their Biomedical Applications
    Review Volume 11, Issue 1, 2021, 8431 - 8445 https://doi.org/10.33263/BRIAC111.84318445 Nanoparticles and their Biomedical Applications Oluwafemi Adeleke Ojo 1,2,* , Israel Idowu Olayide 2 , Maureen Chidinma Akalabu 2, Basiru Olaitan Ajiboye 2 , Adebola Busola Ojo 3 , Babatunji Emmanuel Oyinloye 2,4 , Murugan Ramalingam 5 1 Department of Biochemistry, Landmark University, Omu-Aran, Nigeria 2 Department of Biochemistry, Afe Babalola University, Ado-Ekiti, Nigeria 3 Department of Biochemistry, Ekiti State University, Ado-Ekiti, Nigeria 4 Biotechnology and Structural Biology (BSB) Group, Department of Biochemistry and Microbiology, University of Zululand, KwaDlangezwa 3886, South Africa 5 Biomaterials and stem cell engineering lab, Centre for Biomaterials, Cellular and Molecular Theranostics, School of Mechanical Engineering, Vellore Institute of Technology (VIT) (Deemed to be University), Vellore 632014, India * Correspondence: [email protected]; Scopus Author ID 56415952100 Received: 30.06.2020; Revised: 24.07.2020; Accepted: 26.07.2020; Published: 28.07.2020 Abstract: Over the years, due to the remarkable functional properties, the nanoparticles have been widely used and being tested in the treatment and diagnosis of diseases such as cancer, diabetes, etc. The green synthesis of these nanoparticles can be achieved by physical, chemical, and biological methods. Nanoparticles biosynthesis is put forth to be advantageous over chemical and physical methods because it is non or minimally toxic, environmentally friendly, and cost-effective. A green biosynthesis is an approach that connects nanotechnology with plants, microorganisms, waste materials, and biomolecules. The biological methods help to eliminate destructive processing situations, via letting the synthesis at biological pH, room temperature, and simultaneously, affordable price.
    [Show full text]
  • Laser Scribing Fabrication of Graphitic Carbon Biosensors for Label-Free Detection of Interleukin-6
    nanomaterials Article Laser Scribing Fabrication of Graphitic Carbon Biosensors for Label-Free Detection of Interleukin-6 Pei Shee Tan 1,2,† , Eoghan Vaughan 3,† , Jahidul Islam 3, Niall Burke 1, Daniela Iacopino 3,* and Joanna B. Tierney 1,2 1 Shannon Applied Biotechnology Centre, Munster Technological University, Tralee, V92KA43 Kerry, Ireland; [email protected] (P.S.T.); [email protected] (N.B.); [email protected] (J.B.T.) 2 Department of Biological and Pharmaceutical Sciences, Munster Technological University, Tralee, V92KA43 Kerry, Ireland 3 Tyndall National Institute, University College Cork, Dyke Parade, T12R5CP Cork, Ireland; [email protected] (E.V.); [email protected] (J.I.) * Correspondence: [email protected] † These authors contributed equally. Abstract: Interleukin-6 (IL-6) is an important immuno-modulating cytokine playing a pivotal role in inflammatory processes in disease induction and progression. As IL-6 serves as an important indicator of disease state, it is of paramount importance to develop low cost, fast and sensitive improved methods of detection. Here we present an electrochemical immunosensor platform based on the use of highly porous graphitic carbon electrodes fabricated by direct laser writing of commercial polyimide tapes and chemically modified with capture IL-6 antibodies. The unique porous and 3D morphology, as well as the high density of edge planes of the graphitic carbon electrodes, resulted Citation: Tan, P.S.; Vaughan, E.; in a fast heterogeneous electron transfer (HET) rate, k0 = 0.13 cm/s. The resulting immunosensor Islam, J.; Burke, N.; Iacopino, D.; showed a linear response to log of concentration in the working range of 10 to 500 pg/mL, and Tierney, J.B.
    [Show full text]
  • Cellular Shellization: Surface Engineering Gives Cells an Exterior Review Essays Ben Wang1)Y, Peng Liu1)Y and Ruikang Tang1)2)
    Prospects & Overviews Cellular shellization: Surface engineering gives cells an exterior Review essays Ben Wang1)y, Peng Liu1)y and Ruikang Tang1)2)Ã Unlike eggs and diatoms, most single cells in nature do Introduction not have structured shells to provide extensive protec- tion. It is a challenge to artificially confer shell structures Progress in biological science and materials engineering has begun to blur the boundary between living and non-living on living cells to improve their inherent properties and systems. Synthetic biologists can now engineer complex, arti- functions. We discuss four different types of cellular ficial biological systems to help understand natural biological shellizations: man-made hydrogels, sol-gels, polyelectro- phenomena and use in a variety of biomedical applications [1]. lytes, and mineral shells. We also explore potential appli- Advances in directed evolution and membrane biophysics cations, such as cell storage, protection, delivery, and make the synthesis of simple living cells an imaginable therapy. We suggest that shellization could provide goal [2]. Cellular life is the basic unit of a living organism and another means to regulate and functionalize cells. defines the presence of a stable information reservoir con- Specifically, the integration of living cells and non-living nected to the external world by a well-defined boundary [3]. functional shells may be developed as a novel strategy The cell membrane separates the interior of a cell from the to create ‘‘super’’ or intelligent cells. Unlike biological outside environment and must meet specific requirements approaches, this material-based bio-interface regulation such as semi-permeability to permit communication and molecular transport across the border [4].
    [Show full text]
  • Roadmap on Semiconductor–Cell Biointerfaces
    Physical Biology TOPICAL REVIEW Related content - Progress towards biocompatible Roadmap on semiconductor–cell biointerfaces intracortical microelectrodes for neural interfacing applications Mehdi Jorfi, John L Skousen, Christoph To cite this article: Bozhi Tian et al 2018 Phys. Biol. 15 031002 Weder et al. - Roadmap on neurophotonics Yong Ku Cho, Guoan Zheng, George J Augustine et al. View the article online for updates and enhancements. - Implantable optoelectronic probes for in vivo optogenetics Ege Iseri and Duygu Kuzum Recent citations - Advanced nanostructures for cell membrane poration Apresio K Fajrial and Xiaoyun Ding This content was downloaded from IP address 140.247.113.92 on 11/07/2019 at 17:52 IOP Physical Biology Phys. Biol. 15 (2018) 031002 https://doi.org/10.1088/1478-3975/aa9f34 Phys. Biol. 15 TOPICAL REVIEW 2018 Roadmap on semiconductor–cell biointerfaces 2018 IOP Publishing Ltd RECEIVED © 10 August 2017 Bozhi Tian1 , Shuai Xu2,3, John A Rogers4,5, Stefano Cestellos-Blanco5, Peidong Yang5,6,7,8, REVISED 9 9 10 10 11 11 2 November 2017 João L Carvalho-de-Souza , Francisco Bezanilla , Jia Liu , Zhenan Bao , Martin Hjort , Yuhong Cao , PBHIAT 11 12 13 14 15 16 ACCEPTED FOR PUBLICATION Nicholas Melosh , Guglielmo Lanzani , Fabio Benfenati , Giulia Galli , Francois Gygi , Rylan Kautz , 5 December 2017 Alon A Gorodetsky16,17 , Samuel S Kim18, Timothy K Lu18, Polina Anikeeva19, Michal Cifra20 , 031002 PUBLISHED Ondrej Krivosudský20, Daniel Havelka20 and Yuanwen Jiang1 9 March 2018 1 Department of Chemistry, University of Chicago,
    [Show full text]
  • 1. Introduction
    Chapter 13 STRUCTURAL ORGANISATION AND BIOMIMESIS OF NATURE’S POLYMER COMPOSITES Parvez Alam∗ Centre for Functional Materials, Abo Akademi University, Turku, Finland Abstract In nature, there are many examples of complex hierarchical material designs where weak constituent polymers assemble into composites with exceptional mechanical properties. These structures are attracting considerable attention in the scientific and engineering communities as sources of inspiration for polymer composite design. Nat- ural polymers or, biopolymers, may include proteins, polypeptides and polysaccha- rides. These biopolymers are the essential building blocks of hierarchical polymer composites in nature. This chapter elucidates a variety of different polymer composite structures from nature; how they are organised, and the specialised designs that pro- vide for superior functionality. Recurring concepts in biological material structures will also be explained and hence, the chapter will draw upon the elements of structural biology in context to biomimetic and bioinspired composites science. PACS 82.35.Pq, 87.15.rp, 87.85.jf, 87.15.-v, 81.05.Qk Keywords: Biological structures and biomimesis, biopolymers, biomimetics, mechanics, composites, biostructures AMS Subject Classification: 74R10, 74C10, 74L15 1. Introduction 1.1. Bioinspiration A holistic approach to determining the structure-property relationships of biological materials, most recently termed biomateriomics (Cranford and Buehler 2012), is a neces- ∗E-mail address: parvez.alam@abo.fi (Corresponding Author). Parvez Alam, Adjunct Professor of Com- posite Materials and Biostructures, Centre for Functional Materials, Abo Akademi University, 20500, Turku, Finland. Book: New Developments in Polymer Composites Research; Ed. Laske, S. ISBN: 978-1-62948-343-6 − AUTHOR’S PRE-PRINT 2013 326 Parvez Alam sary precursor to biomimetic and bioinspired materials science.
    [Show full text]
  • 20695837102122127.Pdf
    Volume 10, Issue 2, 2020, 5122 - 5127 ISSN 2069-5837 Biointerface Research in Applied Chemistry www.BiointerfaceResearch.com https://doi.org/10.33263/BRIAC102.122127 Review Article Open Access Journal Received: 04.12.2019 / Revised: 20.01.2020 / Accepted: 23.01.2020 / Published on-line: 28.01.2020 Stimuli-responsive supramolecules for bone tissue engineering 1 1 1, * Akanksha Sharma , Pramod Kumar Sharma , Rishabha Malviya 1Department of Pharmacy, School of Medical and Allied Sciences, Galgotias University, Plot No. 02, Sector 17-A, Greater Noida, Gautam Buddha Nagar, Uttar Pradesh, India *corresponding author e-mail address:rishabhamalviya19@@gmail.com | Scopus ID 36542724200 ABSTRACT In the recent scenario Stimuli responsive supramolecules are used for bone tissue engineering. Stimuli responsive Supramolecules are responding towards the desired stimuli. This has a property to change their dynamics and undergo impulsive and continual assembly or disassembly processes under specific conditions. These supramolecules respond towards chemical and physical stimuli which include: pH, temperature, light, ionic strength, magnetic and electric field sensitive. Stimuli responsive supramolecules are used to various preparations such as hydrogels, scaffolds, hydrogel scaffolds, 3D bioprinting, 4D bioprinting, nanogels and microgels used for the bone tissue repair and regenerative medicine. Manuscript deals with various approaches used to prepare stimuli responsive supramolecules for bone engineering applications. Keywords: Stimuli; engineering; supramolecules; hydrogels; scffolds; hydrogel scaffolds; bone tissue engineering. 1. INTRODUCTION Recently the stimuli-responsive supramolecular assemblies physical or reversible gel and covalently cross-linked gels received attention because their structures can be modified by [6].Tissue engineering is the application of chemical, biological applying a stimulus. They can be reverting back towards their and engineering principles towards the repair, regeneration or original structures by applying another stimulus.
    [Show full text]
  • Engineering the Biointerface for Enhanced Bioelectrode
    1 ENGINEERING THE BIOINTERFACE FOR ENHANCED 2 BIOELECTRODE AND BIOSENSOR PERFORMANCE 3 BY BUDDY D. RATNER 4 5 THE MODERATOR: I'D AGAIN LIKE TO WELCOME 6 YOU TO THE SECOND OF THE SERIES OF TUTORIAL SESSIONS 7 FOR THE NATIONAL ACADEMIES KECK FUTURES INITIATIVE ON 8 SMART PROSTHETICS. IT'S MY PLEASURE TO INTRODUCE OUR 9 SECOND SPEAKER, DR. BUDDY RATNER. HE'S THE DIRECTOR OF 10 THE UNIVERSITY OF WASHINGTON ENGINEERED BIOMATERIALS 11 AND PROFESSOR OF BIOENGINEERING AND CHEMICAL 12 ENGINEERING AT THE UNIVERSITY OF WASHINGTON. HE'LL BE 13 SPEAKING ABOUT ENGINEERING THE BIOINTERFACE FOR 14 ENHANCED BIOELECTRODE AND BIOSENSOR PERFORMANCE. 15 DR. RATNER: GOOD MORNING. PLEASURE TO BE 16 ABLE TO PRESENT SOME PERSPECTIVES, I THINK, FROM COMING 17 IN THE DIRECTION, PERHAPS, THE WAY A BIOENGINEER MIGHT 18 THINK ABOUT THIS INTERFACE. 19 AND LET'S SEE. SO WHAT WE SOMETIMES REFER TO 20 AS THE BIOINTERFACE, IF THIS GREEN DEVICE IN THE CENTER 21 OF THE SLIDE IS SOME EITHER SENSOR OR ELECTRODE AND IT 22 EXISTS IN SOME BIOLOGICAL ENVIRONMENT, I'VE DRAWN IT IN 23 AN AQUEOUS, MAYBE AN OCEANIC ENVIRONMENT, BUT IT COULD 24 JUST AS WELL BE IN A BRAIN OR IN A SOFT TISSUE. AT THE 25 INTERFACE THERE'S SOME VERY INTERESTING EVENTS THAT 1 1 TAKE PLACE. 2 AND THIS SLIDE, IT'S A RATHER LENGTHY SLIDE, 3 BUT IT COVERS, I THINK, THE MAJORITY OF THE WAYS THIS 4 INTERESTING ENVIRONMENT, THE BIOENVIRONMENT, CAN IMPACT 5 SYNTHETIC MATERIALS WE PUT INTO THE BODY. I'M NOT 6 GOING TO ACTUALLY READ EVERY ONE OF THEM.
    [Show full text]
  • University of Groningen the Effect of Wettability and Stiffness on Stem Cell
    University of Groningen The effect of wettability and stiffness on stem cell behavior at bioinerfaces Kühn, Philipp Till IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from it. Please check the document version below. Document Version Publisher's PDF, also known as Version of record Publication date: 2016 Link to publication in University of Groningen/UMCG research database Citation for published version (APA): Kühn, P. T. (2016). The effect of wettability and stiffness on stem cell behavior at bioinerfaces. Rijksuniversiteit Groningen. Copyright Other than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons). Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum. Download date: 24-09-2021 CHAPTER ONE GENERAL INTRODUCTION 1 GENERAL INTRODUCTION 1.1. BIOINTERFACES Continuous improvement of quality of life is one of the essential features of modern society. With increasing average age, our society faces a growing number of health related necessary improvements of this life quality. For that reason, a vast amount of research is conducted to find new biomaterials with improved performance preferably at low costs.
    [Show full text]
  • Special Issue on Nano-Biointerface for Biosensing
    applied sciences Editorial Special Issue on Nano-Biointerface for Biosensing Cristina Satriano Laboratory of Hybrid NanobioInterfaces (NHIL), Department of Chemical Sciences, University of Catania, viale Andrea Doria 6, 95125 Catania, Italy; [email protected]; Tel.: +39-095-738-5136 Received: 17 October 2019; Accepted: 21 October 2019; Published: 24 October 2019 1. Introduction Point-of-care nanobiosensors have tremendous potential to revolutionize the future of personalized nanomedicine, especially for the simultaneous diagnosis and therapy, which takes the name of theranostics. Nanoscale-sized biosensors offer several advantages compared to traditional biosensors, including the enhanced loading of desired biomolecules due to their larger surface area; the multifaceted properties of nanostructured materials, thus the capability for simultaneous measurements of multiple biomarkers; and the increased sensitivity for the detection of a minute biomolecule concentration. With the rapid growth of nanotechnology, an increased interest has focused on the development of engineered nanomaterials suitable for a triggered interaction at the biological interface, for the development of novel sensing devices targeted to analytes ranging from small molecules to proteins to oligonucleotides. In general, functionalized nanomaterials, owing to their unique and tunable electrochemical and optical properties, are very advantageous as immobilization platforms, catalytic tools, and optical and electroactive labels to enhance biosensing characteristics, such as sensitivity, stability, and selectivity [1]. Additionally, the incorporation of multiple nanomaterials into composite films can take advantage of the superior and potentially synergistic properties of each component. Different detection methods, including those based on fluorescence or electrochemistry or surface plasmon resonance or acoustic evanescent waves, have advanced through using new nanomaterials prepared in a wide range of different structures.
    [Show full text]
  • S41598-021-82648-9.Pdf
    www.nature.com/scientificreports OPEN Self‑assembling thermostable chimeras as new platform for arsenic biosensing Rosanna Puopolo1, Ilaria Sorrentino2, Giovanni Gallo1, Alessandra Piscitelli3, Paola Giardina3, Alan Le Gof2* & Gabriella Fiorentino1* The correct immobilization and orientation of enzymes on nanosurfaces is a crucial step either for the realization of biosensors, as well as to guarantee the efcacy of the developed biomaterials. In this work we produced two versions of a chimeric protein, namely ArsC‑Vmh2 and Vmh2‑ ArsC, which combined the self‑assembling properties of Vmh2, a hydrophobin from Pleurotus ostreatus, with that of TtArsC, a thermophilic arsenate reductase from Thermus thermophilus; both chimeras were heterologously expressed in Escherichia coli and purifed from inclusion bodies. They were characterized for their enzymatic capability to reduce As(V) into As(III), as well as for their immobilization properties on polystyrene and gold in comparison to the native TtArsC. The chimeric proteins immobilized on polystyrene can be reused up to three times and stored for 15 days with 50% of activity loss. Immobilization on gold electrodes showed that both chimeras follow a classic Langmuir isotherm model towards As(III) recognition, with an association constant (KAsIII) between As(III) and the immobilized enzyme, equal to 650 (± 100) L mol−1 for ArsC‑Vmh2 and to 1200 (± 300) L mol−1 for Vmh2‑ArsC. The results demonstrate that gold‑immobilized ArsC‑Vmh2 and Vmh2‑ ArsC can be exploited as electrochemical biosensors to detect As(III). Arsenic (As) is a toxic metalloid widespread in soil, water and air, harmful to humans and the environment 1,2; due to its toxicity, one biotechnological challenge is centred on the development of biosensors to monitor its concentration in the environment3.
    [Show full text]
  • Nucleic Acids Analysis SCIENCE CHINA
    SCIENCE CHINA Chemistry •INVITED REVIEWS• February 2021 Vol.64 No.2: 171–203 https://doi.org/10.1007/s11426-020-9864-7 Nucleic acids analysis Yongxi Zhao1†, Xiaolei Zuo2†, Qian Li3†, Feng Chen1, Yan-Ru Chen4, Jinqi Deng5, Da Han2, Changlong Hao6, Fujian Huang7, Yanyi Huang8, Guoliang Ke9, Hua Kuang6, Fan Li2, Jiang Li10,11, Min Li2, Na Li12, Zhenyu Lin13, Dingbin Liu14, Juewen Liu15, Libing Liu16, Xiaoguo Liu3, Chunhua Lu13, Fang Luo13, Xiuhai Mao2, Jiashu Sun5, Bo Tang12, Fei Wang3, Jianbin Wang17, Lihua Wang10,11, Shu Wang15, Lingling Wu2, Zai-Sheng Wu4, Fan Xia7, Chuanlai Xu6, Yang Yang2, Bi-Feng Yuan18, Quan Yuan9, Chao Zhang2, Zhi Zhu19, Chaoyong Yang2,19*, Xiao-Bing Zhang9*, Huanghao Yang13*, Weihong Tan2,9* & Chunhai Fan2,3* 1Institute of Analytical Chemistry and Instrument for Life Science, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, China; 2Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China; 3School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules, Institute of Translational Medicine, Shanghai Jiao Tong University, Shanghai 200240, China; 4Cancer Metastasis Alert and Prevention Center, Fujian Provincial Key Laboratory of Cancer Metastasis Chemoprevention and Chemotherapy, State Key Laboratory of Photocatalysis on Energy and Environment,
    [Show full text]