EP) Study and Radiofrequency Ablation (RFA
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Electrophysiology-Appnote.Pdf
Application Note: Electrophysiology Electrophysiology Introduction Electrophysiology is a field of research that deals with the electrical properties of cells and biological tissues. In some cases, it is used to test for nervous or cardiac diseases and abnormalities. In many research applications probes are used to measure the electrical activity of individual cells, tissues, and whole specimens. Measuring the activity of cells at various membrane potentials can give valuable information about ion transport mechanisms and cellular communication. Varying ionic strength and membrane potential on cell populations can be used to study contractile movements of muscle cells, and diseases affecting the normal propagation of impulses. Using various stimulation techniques along with a selection of quality equipment and setup design can give rise to a wealth of applications in electrophysiology. Electrophysiology Principle Researchers and clinicians use electrophysiology when studying the electrical properties of neural and muscle tissue. In the clinical laboratory, electroencephalograms are routinely performed as a test for neural disorders like epilepsy, brain tumor, stroke, encephalitis, and others by measuring the electrical activity of the brain through external electrodes. In the research laboratory, electrophysiology methods are used to measure the ion-channel activity of cell membranes in various electrical environments. Here, an extremely thin micropipette is used to make intimate contact with the cell membrane to study membrane potential. Neurons and other cell types derive their electrical properties from their lipid bilayer and the ion concentrations inside and outside of the cell. The ion concentration differences create a membrane potential difference on either side of the membrane. The flow of ions across the membrane generates a current that can be measured using Ohm’s law, where the change in voltage (V) is related to the current (I) and membrane resistance (R). -
Supraventricular Tachycardia: from Fetus to Adult
Supraventricular Tachycardia: From Fetus to Adult Mohamed Hamdan, MD Learning Objectives Define type of SVT by age Describe clinical approach Describe prenatal and postnatal management of SVT 2 SVT Across the Ages Narrow-complex Wide complex: VT Tachycardia HR >160: fetuses HR >220: infants HR >180: children HR >120: adults Above AV junction Types of SVT Re-entrant Automatic 1. Sudden onset and 1. Warms-up and cools offset down 2. Accessory pathway 2. Sensitive to 3. Terminates with catecholamines adenosine or 3. Does not respond to cardioversion either Most common: AVRT Most common: EAT Mechanism of SVT Re-entrant Automatic 90% <1% 10% AVN dependant AVN independant AVRT Atrial flutter AET AVNRT Atrial fib. CAR PJRT SNRT JET Mahaim Tachycardia IART 5 Re-entrant Automatic Fetal SVT Definition HR > 160 not associated with contraction or periodic decelerations Epidemiology Incidence ~1/1000 of all pregnancies Fetal arrhythmia: 1/3 of referrals to fetal echo lab CHD in 3% Copel et al., Am J Obstet Gynecol 2000 7 Clinical Approach to Fetal SVT 1. Define type SVT vs Flutter vs Fibrillation 2. Define absolute HR 3. Presence of CHD 4. Presence of hydrops 8 1. Type of SVT Best: 16-32 weeks Methods Phonocardiography Fetal echo Magnetocardiography (MCG) Fetal scalp electrode Fetal QRS Maternal QRS 9 Assessment of Rhythm by Fetal Echo Doppler SVC Aorta PAC Normal 10 Assessment of Rhythm by Fetal Echo M-Mode RV RA Atrium Rate~ 500 Ventricle Rate~240 11 2. Determine HR Atrial Rate Variability A:V Ventricular (bpm) Conduction Rate Normal 120-160 >10 bpm 1:1 120-160 Sinus 180-220 5-15 bpm 1:1 180-220 tachycardia SVT >220 No 1:1 >220 Atrial flutter 300-500 No 2-5:1 60-200 Atrial 300-500 Yes, due to 2-5:1 60-200 fibrillation variable AV conduction Bergmans et al., Obstet Gynecol Surv 1985 12 3. -
Brain Slice Preparation in Electrophysiology
Brain Slice Preparation In structural integrity, unlike cell cultures or tissue homogenates. Electrophysiology Some of the limitations of these preparations are: 1) lack of certain inputs and outputs normally existing in the Avital Schurr, Ph.D. intact brain; 2) certain portions of the sliced tissue, Department of Anesthesiology, especially the top and bottom surfaces of the slice, are University of Louisville School of Medicine, damaged by the slicing action itself; 3) the life span of a Louisville, Kentucky 40292 brain slice is limited and the tissue gets "older" at a much faster rate than the whole animal; 4) the effects of Avital Schurr is currently an Associate Professor in the decapitation ischemia on the viability of the slice are not Department of Anesthesiology at the University of well understood; 5) Since blood-borne factors may be Louisville. He received his Ph.D. in 1977 from Ben- missing from the artificial bathing medium of the brain Gurion University in Beer Sheva, Israel. From 1977 slice, they cannot benefit the preparation and thus the through 1981, he held two postdoctoral positions, one optimal composition of the bathing solution is not yet at the Baylor College of Medicine and the second at established. the University of Texas Medical School at Houston. Brain slice preparations are becoming PREPARATION OF SLICES increasingly popular among neurobiologists for the In general, rodents are the animals of choice for the study of the mammalian central nervous system preparation of brain slices. Of those, the rat and the guinea pig are the most used. After decapitation, the (CNS) in general and synaptic phenomena in brain is removed rapidly from the skull and rinsed with particular. -
Thursday Poster Assignment
Corresponding Author Paper Title Board Assignment Number ‐ Thursday Classification of Typical Developing and Autism Spectrum Disorder Using Connectivity A.R., Jac Fredo Matrix and Support Vector Machine 111 Abbaszadeh, Behrooz Probabilistic Prediction of Epileptic Seizures Using SVM 220 Abe, Takuto Surrogate Modeling for Neuroprotective Focal Brain Cooling Device 110 Comprehensive Comparison of 2D vs. 3D Resource Usage in Large Volumetric Medical Agris, Jacob Image Segmentation 109 On Smartphone Sensability of Bi‐Phasic User Intoxication Levels from Diverse Walk Agu, Emmanuel Types in Standardized Field Sobriety Tests 327 The Effect of Perceived Sound Quality of Speech in Noisy Speech Perception by Akbarzadeh, Sara Normal Hearing and Hearing Impaired Listeners 370 Towards the Development of an Optrode Biopotential Sensor: Characterization Using Al Abed, Amr in Vitro Cardiac Tissue Recordings 108 Spatially Filtered Low‐Density EMG and Time‐Domain Descriptors Improves Hand Al‐Jumaily, Adel Movement Recognition 419 Optimizing Stimulation Strategies for Retinal Electrical Stimulation: A Modelling Study Alqahtani, Abdulrahman 287 An Automatic Navigation and Pressure Monitoring for Guided Insertion Procedure Alsunaydih, Fahad Nasser 326 A Statistical Determination of the Energy Delivered to Muscular Tissue in Amador, Alejandro Electrostimulation Protocols 149 Analysis of Muscle Fatigue During Exercise and Exercise Combined with Electrostimulation 151 Signal‐To‐Noise Ratio Determination in a Hybrid System of Electrostimulation and Electromiography 150 Continuous Prediction of Cognitive State Using a Marked‐Point Process Modeling Amidi, Yalda Framework 286 Antônio Freire Teixeira, Marcos Automatic Counting of Erythrocytes Using Image Processing 153 Feature Extraction from Radiographic Images for Bone Age Identification 152 Arantes, Ana Paula Bittar Britto Towards the Improvement of Algorithms Used in Robot‐Assisted Therapies 107 Arce‐Diego, José L. -
Transcatheter Ablation for the Treatment of Supraventricular Tachycardia in Adults
Medical Coverage Policy Effective Date ............................................. 4/15/2021 Next Review Date ....................................... 4/15/2022 Coverage Policy Number .................................. 0529 Transcatheter Ablation for the Treatment of Supraventricular Tachycardia in Adults Table of Contents Related Coverage Resources Overview .............................................................. 1 Cardiac Electrophysiological (EP) Studies Coverage Policy ................................................... 1 Nonpharmacological Treatments for Atrial Fibrillation General Background ............................................ 2 Medicare Coverage Determinations .................. 12 Coding/Billing Information .................................. 12 References ........................................................ 13 INSTRUCTIONS FOR USE The following Coverage Policy applies to health benefit plans administered by Cigna Companies. Certain Cigna Companies and/or lines of business only provide utilization review services to clients and do not make coverage determinations. References to standard benefit plan language and coverage determinations do not apply to those clients. Coverage Policies are intended to provide guidance in interpreting certain standard benefit plans administered by Cigna Companies. Please note, the terms of a customer’s particular benefit plan document [Group Service Agreement, Evidence of Coverage, Certificate of Coverage, Summary Plan Description (SPD) or similar plan document] may differ significantly -
Radiofrequency Ablation: Technique and Clinical Applications
Diagn Interv Radiol 2012; 18:508–516 INTERVENTIONAL RADIOLOGY © Turkish Society of Radiology 2012 REVIEW Radiofrequency ablation: technique and clinical applications Servet Tatlı, Ümit Tapan, Paul R. Morrison, Stuart G. Silverman ABSTRACT n last decade, there has been a rapid advancement in the utiliza- Radiofrequency ablation is the most commonly used percu- tion of percutaneous, image-guided tumor ablation methods. taneous ablation technique and well-documented in the lit- erature on focal therapies. It has become the image-guided I Radiofrequency (RF) ablation has become the method of choice be- ablation method of choice because of its efficacy, safety, and cause of its safety and efficacy. Image-guided RF ablation is minimally ease of use. Radiofrequency ablation has shown promise in treating selected solid tumors, particularly those involving the invasive and usually appropriate for inoperable patients with other co- liver, kidneys, lungs, and the musculoskeletal system. It is a morbidities. It requires a minimal hospital stay or can be performed on minimally invasive technique often used in inoperable patients an outpatient basis. It preserves more normal organ tissue and is less with other comorbidities. Radiofrequency ablation requires a minimal hospital stay or can be performed on an outpatient expensive than surgery (1–3). The procedures are generally performed by basis. The aim of this article is to review radiofrequency abla- using 14–21 G, partially insulated electrodes that are placed under guid- tion techniques and their clinical applications. ance (computed tomography [CT], magnetic resonance imaging [MRI], Key words: • radiofrequency catheter ablation • tumor or ultrasonography [US]) into the tumor to be ablated. -
Electrophysiology Read-Out Tools for Brain-On-Chip Biotechnology
micromachines Review Electrophysiology Read-Out Tools for Brain-on-Chip Biotechnology Csaba Forro 1,2,†, Davide Caron 3,† , Gian Nicola Angotzi 4,†, Vincenzo Gallo 3, Luca Berdondini 4 , Francesca Santoro 1 , Gemma Palazzolo 3,* and Gabriella Panuccio 3,* 1 Tissue Electronics, Fondazione Istituto Italiano di Tecnologia, Largo Barsanti e Matteucci, 53-80125 Naples, Italy; [email protected] (C.F.); [email protected] (F.S.) 2 Department of Chemistry, Stanford University, Stanford, CA 94305, USA 3 Enhanced Regenerative Medicine, Fondazione Istituto Italiano di Tecnologia, Via Morego, 30-16163 Genova, Italy; [email protected] (D.C.); [email protected] (V.G.) 4 Microtechnology for Neuroelectronics, Fondazione Istituto Italiano di Tecnologia, Via Morego, 30-16163 Genova, Italy; [email protected] (G.N.A.); [email protected] (L.B.) * Correspondence: [email protected] (G.P.); [email protected] (G.P.); Tel.: +39-010-2896-884 (G.P.); +39-010-2896-493 (G.P.) † These authors contributed equally to this paper. Abstract: Brain-on-Chip (BoC) biotechnology is emerging as a promising tool for biomedical and pharmaceutical research applied to the neurosciences. At the convergence between lab-on-chip and cell biology, BoC couples in vitro three-dimensional brain-like systems to an engineered microfluidics platform designed to provide an in vivo-like extrinsic microenvironment with the aim of replicating tissue- or organ-level physiological functions. BoC therefore offers the advantage of an in vitro repro- duction of brain structures that is more faithful to the native correlate than what is obtained with conventional cell culture techniques. -
Electrophysiology Study
Electrophysiology (EP) Study Highly trained specialists perform EP studies in a specially designed EP lab outfitted with advanced technology and equipment. Why an EP study? The Value of an EP Study While electrocardiograms (ECGs An electrophysiology, or EP, study or EKGs) are important tests of the provides information that is key to heart’s electrical system, they diagnosing and treating arrhythmias. provide only a brief snapshot of Although it is more invasive than an the heart’s electrical activity. electrocardiogram (ECG) or echocar - Arrhythmias can be unpredictable diogram, and involves provoking and intermittent, which makes it arrhythmias, the test produces data unlikely that an electrocardiogram that makes it possible to : will capture the underlying electri - Normally, electricity flows through - cal pathway problem. Even tests • Diagnose the source of arrhythmia out the heart in a regular, meas - that stretch over longer time periods , symptoms such as Holter monitoring, may not ured pattern. This electrical system • Evaluate the effectiveness of capture an event. brings about coordinated heart certain medications in controlling muscle contractions. A problem During an EP study, a specially the heart rhythm disorder anywhere along the electrical trained cardiac specialist may pro - • Predict the risk of a future cardiac pathway causes an arrhythmia, voke arrhythmia events and collect event, such as Sudden Cardiac or heart rhythm disturbance. By data about the flow of electricity Death accurately diagnosing the precise during actual events. As a result, cause of an arrhythmia, it is possi - • Assess the need for an implantable EP studies can diagnose the ble to select the best possible device (a pacemaker or ICD) or cause and precise location of the treatment. -
Artificial Pneumothorax Improves Radiofrequency Ablation Of
Zuo et al. BMC Cancer (2021) 21:505 https://doi.org/10.1186/s12885-021-08223-7 RESEARCH ARTICLE Open Access Artificial pneumothorax improves radiofrequency ablation of pulmonary metastases of hepatocellular carcinoma close to mediastinum Taiyang Zuo1,2†, Wenli Lin1†, Fengyong Liu1,2 and Jinshun Xu1,2,3* Abstract Background: To investigate the feasibility, safety and efficacy of percutaneous radiofrequency ablation (RFA) of pulmonary metastases from hepatocellular carcinoma (HCC) contiguous with the mediastinum using the artificial pneumothorax technique. Method: A total of 40 lesions in 32 patients with pulmonary metastases from HCC contiguous with the mediastinum accepted RFA treatment from August 2014 to May 2018 via the artificial pneumothorax technique. After ablation, clinical outcomes were followed up by contrast enhanced CT. Technical success, local tumor progression (LTP), intrapulmonary distant recurrence (IDR), and adverse events were evaluated. Overall survival (OS) and local tumor progression free survival (LTPFS) were recorded for each patient. Results: The tumor size was 1.4 ± 0.6 cm in diameter. RFA procedures were all successfully performed without intra- ablative complications. Technical success was noted in 100% of the patients. Five cases of LTP and 8 cases of IDR occurred following the secondary RFA for treatment. Slight pain was reported in all patients. No major complications were observed. The 1, 2, and 3-year LTPFS rates were 90.6, 81.2, and 71.8%, and the 1, 2, and 3-year OS rates were 100, 100 and 87.5%, respectively. Conclusion: Artificial pneumothorax adjuvant RFA is a feasible, safe, and efficient method for treatment of pulmonary metastases from HCC contiguous with the mediastinum. -
Molecular Mechanisms of Mechanoperception in Plants Gabriele B
Washington University in St. Louis Washington University Open Scholarship Biology Faculty Publications & Presentations Biology 8-2013 A force of nature: molecular mechanisms of mechanoperception in plants Gabriele B. Monshausen Pennsylvania State University - Main Campus Elizabeth S. Haswell Washington University in St Louis, [email protected] Follow this and additional works at: https://openscholarship.wustl.edu/bio_facpubs Part of the Biology Commons, Biophysics Commons, Cell Biology Commons, and the Plant Sciences Commons Recommended Citation Monshausen, Gabriele B. and Haswell, Elizabeth S., "A force of nature: molecular mechanisms of mechanoperception in plants" (2013). Biology Faculty Publications & Presentations. 38. https://openscholarship.wustl.edu/bio_facpubs/38 This Article is brought to you for free and open access by the Biology at Washington University Open Scholarship. It has been accepted for inclusion in Biology Faculty Publications & Presentations by an authorized administrator of Washington University Open Scholarship. For more information, please contact [email protected]. A Force of Nature: Molecular Mechanisms of Mechanoperception in Plants Elizabeth S. Haswell1 and Gabriele B. Monshausen2 1Department of Biology, Washington University in St. Louis, St. Louis, MO 63130, USA 2Biology Department, Pennsylvania State University, University Park, Pa 16802, USA To whom correspondence should be addressed: [email protected]; [email protected] Abstract The ability to sense and respond to a wide variety of mechanical stimuli—gravity, touch, osmotic pressure, or the resistance of the cell wall—is a critical feature of every plant cell, whether or not it is specialized for mechanotransduction. Mechanoperceptive events are an essential part of plant life, required for normal growth and development at the cell, tissue and whole-plant level and for the proper response to an array of biotic and abiotic stresses. -
A Single-Neuron: Current Trends and Future Prospects
cells Review A Single-Neuron: Current Trends and Future Prospects Pallavi Gupta 1, Nandhini Balasubramaniam 1, Hwan-You Chang 2, Fan-Gang Tseng 3 and Tuhin Subhra Santra 1,* 1 Department of Engineering Design, Indian Institute of Technology Madras, Tamil Nadu 600036, India; [email protected] (P.G.); [email protected] (N.B.) 2 Department of Medical Science, National Tsing Hua University, Hsinchu 30013, Taiwan; [email protected] 3 Department of Engineering and System Science, National Tsing Hua University, Hsinchu 30013, Taiwan; [email protected] * Correspondence: [email protected] or [email protected]; Tel.: +91-044-2257-4747 Received: 29 April 2020; Accepted: 19 June 2020; Published: 23 June 2020 Abstract: The brain is an intricate network with complex organizational principles facilitating a concerted communication between single-neurons, distinct neuron populations, and remote brain areas. The communication, technically referred to as connectivity, between single-neurons, is the center of many investigations aimed at elucidating pathophysiology, anatomical differences, and structural and functional features. In comparison with bulk analysis, single-neuron analysis can provide precise information about neurons or even sub-neuron level electrophysiology, anatomical differences, pathophysiology, structural and functional features, in addition to their communications with other neurons, and can promote essential information to understand the brain and its activity. This review highlights various single-neuron models and their behaviors, followed by different analysis methods. Again, to elucidate cellular dynamics in terms of electrophysiology at the single-neuron level, we emphasize in detail the role of single-neuron mapping and electrophysiological recording. We also elaborate on the recent development of single-neuron isolation, manipulation, and therapeutic progress using advanced micro/nanofluidic devices, as well as microinjection, electroporation, microelectrode array, optical transfection, optogenetic techniques. -
Nitric Oxide Resets Kisspeptin-Excited Gnrh Neurons Via PIP2 Replenishment
Nitric oxide resets kisspeptin-excited GnRH neurons via PIP2 replenishment Stephanie Constantina, Daniel Reynoldsa, Andrew Oha, Katherine Pizanoa, and Susan Wraya,1 aCellular and Developmental Neurobiology Section, National Institute of Neurological Disorders and Stroke (NINDS), NIH, Bethesda, MD 20892 Edited by Solomon H. Snyder, Johns Hopkins University School of Medicine, Baltimore, MD, and approved November 23, 2020 (received for review June 15, 2020) 2+ Fertility relies upon pulsatile release of gonadotropin-releasing rate (20). Under normal conditions, [Ca ]i oscillations are driven hormone (GnRH) that drives pulsatile luteinizing hormone secre- by bursts of action potentials (AP) (21, 22). Yet, AP are not 2+ tion. Kisspeptin (KP) neurons in the arcuate nucleus are at the necessary for the KP-evoked [Ca ]i response to occur, as it is center of the GnRH pulse generation and the steroid feedback driven by multiple effectors including transient receptor potential- control of GnRH secretion. However, KP evokes a long-lasting re- canonical channels (TRPC), voltage-gated calcium channels sponse in GnRH neurons that is hard to reconcile with periodic (VGCC), and inositol 1,4,5-trisphosphate receptors (InsP3R) (15, GnRH activity required to drive GnRH pulses. Using calcium imag- 16, 19, 23). Thus, the versatility of Kiss1r signaling pathway un- ing, we show that 1) the tetrodotoxin-insensitive calcium response derlies the functionality of KP projections along GnRH neuron evoked by KP relies upon the ongoing activity of canonical tran- processes (24), with KP locally applied on nerve terminals also 2+ sient receptor potential channels maintaining voltage-gated evoking a long-lasting increase in [Ca ]i (16).