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Us 2018 / 0237793 A1 US 20180237793A1 ( 19) United States (12 ) Patent Application Publication ( 10) Pub . No. : US 2018 /0237793 A1 Aasen et al. (43 ) Pub . Date : Aug. 23, 2018 ( 54 ) TRANSGENIC PLANTS WITH ENHANCED Tyamagondlu V . Venkatesh , St . Louis , AGRONOMIC TRAITS MO (US ) ; Huai Wang, Chesterfield , MO (US ) ; Jingrui Wu , Johnston , IA (71 ) Applicant: Monsanto Technology LLC , St. Louis , (US ) ; Xiaoyun Wu, Chesterfield , MO MO (US ) (US ) ; Xiaofeng Sean Yang , Chesterfield , MO (US ) ; Qin Zeng , ( 72 ) Inventors : Eric D . Aasen , Woodland , CA (US ) ; Maryland Heights , MO (US ) ; Jianmin Mark Scott Abad , Webster Groves , Zhao , St . Louis , MO (US ) MO (US ) ; Edwards Allen , O ' Fallon , MO (US ) ; Thandoni Rao Ambika , (73 ) Assignee: Monsanto Technology LLC , St. Louis , Karnataka (IN ) ; Veena S . Anil , MO (US ) Bangalore ( IN ) ; Alice Clara Augustine , (21 ) Appl. No .: 15 /827 , 886 Karnataka ( IN ) ; Stephanie L . Back , St. Louis , MO (US ) ; Pranesh Badami, (22 ) Filed : Nov . 30 , 2017 Bangalore (IN ) ; Amarjit Basra , Chesterfield , MO (US ) ; Kraig L . Related U . S . Application Data Brustad , Saunderstown , RI (US ) ; (63 ) Continuation of application No . 14 /632 , 018 , filed on Molian Deng, Grover , MO (US ) ; Todd Feb . 26 , 2015 , now abandoned , which is a continu Dezwaan , Apex , NC (US ) ; Charles ation of application No. 12 /998 ,243 , filed on Sep . 16 , Dietrich , Chesterfield , MO (US ) ; 2011 , now abandoned , filed as application No . PCT/ Stephen M . Duff , St. Louis , MO (US ) ; US2009/ 058908 on Sep . 30 , 2009 . Karen K . Gabbert , St. Louis , MO ( 60 ) Provisional application No . 61/ 226 , 953 , filed on Jul . ( US ) ; Steve He, St. Louis , MO (US ) ; 20 , 2009 , provisional application No . 61 / 182 ,785 , Bill L . Hendrix , West Sacramento , CA (US ) ; Hongwu Jia , Creve Coeur , MO filed on Jun . 1 , 2009 , provisional application No . (US ) ; Vidya Kammaradi Ramanath 61 / 164 ,664 , filed on Mar . 30 , 2009 , provisional ap Bhat, Karnataka ( IN ) ; Balasulojini plication No. 61/ 155 , 950 , filed on Feb . 27 , 2009 , Karunanandaa , Creve Coeur, MO provisional application No . 61/ 148 ,438 , filed on Jan . ( US ) ; Kevin R . Kosola , Wildwood , MO 30 , 2009 , provisional application No . 61 / 139 , 164 , (US ) ; Kempagangaiah filed on Dec . 19 , 2008 , provisional application No . Krishnamurthy , Karnataka ( IN ) ; 61 / 101 ,722 , filed on Oct. 1 , 2008 . Ganesh Kumar , Chesterfield , MO Publication Classification (US ) ; Saritha V . Kuriakose , Kerela ( IN ) ; Shantala Lakkanna, Bangalore (51 ) Int. CI. ( IN ) ; Mark E . Leibman , St. Louis , MO C12N 15/ 82 ( 2006 .01 ) ( US ) ; Linda Lutfiyya, St. Louis , MO 2 ) U . S . CI. (US ) ; Savitha Madappa , Karnataka CPC . .. C12N 15 / 8251 ( 2013 .01 ) ; C12N 15 /8274 ( IN ) ; Sivalinganna Manjunath , ( 2013 .01 ) ; C12N 15 /8273 ( 2013 .01 ) ; YO2A Chesterfield , MO (US ) ; Robert J . 40 / 146 (2018 .01 ) ; C12N 15 /8261 ( 2013 .01 ) ; Meister , St. Peters, MO (US ) ; Bellur C12N 15 /8247 ( 2013 .01 ) ; C12N 15 /8275 Narasimha Prasad , Bangalore ( IN ) ; (2013 .01 ) Sasha Preuss , Webster Groves, MO (57 ) ABSTRACT (US ) ; Qungang Qi, Chesterfield , MO (US ) ; Dhanalakshmi Ramachandra , This invention provides transgenic plant cells with recom Bangalore ( IN ) ; Aniruddha binant DNA for expression of proteins that are useful for Raychaudhuri, Wildwood , MO (US ); imparting enhanced agronomic trait ( s ) to transgenic crop S . Sangeetha , Karnataka ( IN ) ; Scott plants . This invention also provides transgenic plants and Saracco , St. Louis , MO (US ) ; Chitresh progeny seed comprising the transgenic plant cells where the Sharma, Himachal Pradesh (IN ) ; Vijay plants are selected for having an enhanced trait selected K . Sharma, Wildwood , MO ( US ) ; from the group of traits consisting of enhanced water use Monnanda S . Rajani, St. Louis , MO efficiency , enhanced cold tolerance , increased yield , (US ) ; Padmini Sudarshana , Bangalore enhanced nitrogen use efficiency , enhanced seed protein and ( IN ) ; Rebecca L . Thompson -Mize , St. enhanced seed oil . Also disclosed are methods for manu Charles , MO (US ) ; Srikanth facturing transgenic seed and plants with enhanced trait . Venkatachalayya , Karnataka (IN ) ; Specification includes a Sequence Listing . US 2018 /0237793 A1 Aug . 23 , 2018 TRANSGENIC PLANTS WITH ENHANCED 366 - 372 , 374 - 383 , 389 - 392 , 394 , 396 , 401 - 403 , 405 -412 , AGRONOMIC TRAITS 414 , 417 -424 , 427 -453 , 455 -473 , 475 , 488 -501 , 503 - 517 , 519 - 531, 533 - 540 , 542 - 543 , and 546 -614 or that are CROSS REFERENCE TO RELATED homologous to a sequence selected from the group consist APPLICATIONS ing of SEQ ID NOs: 308 , 310 , 312 - 315 , 317 - 323 , 325 - 343 , [ 0001] This application claims benefit under 35 USC § 345 , 347 -349 , 352 - 354 , 356 , 358 -359 , 366 - 372 , 374 -383 , 119 ( e ) of U . S . provisional application Ser . No. 61/ 101 , 722 , 389 - 392 , 394 , 396 , 401 - 403 , 405 - 412 , 414 , 417 - 424 , 427 filed Oct. 1 , 2008 , 61 / 139 , 164 , filed Dec . 19 , 2008 , 61/ 148 , 453 , 455 -473 , 475 , 488 -501 , 503 -517 , 519 -531 , 533 - 540 , 438 , filed Jan . 30 , 2009 , 61/ 155 ,950 , filed Feb . 27 , 2009 , 542 - 543 , and 546 -614 . 61/ 164 ,664 , filed Mar . 30 , 2009, 61 / 182 , 785 , filed Jun . 1 , [0006 ] In other aspects of the invention the recombinant 2009 and 61 / 226 , 953 , filed Jul. 20 , 2009, each of which is DNA constructs provide for suppression of a native protein . incorporated herein by reference in its entirety . In those other aspects of the invention the recombinant DNA constructs are characterized as being constructed with sense INCORPORATION OF SEQUENCE LISTING oriented and anti - sense - oriented polynucleotides , e . g . poly nucleotides derived from genes having SEQ ID NOs: 2 , 4 , [0002 ] The sequence listing file named “ 56202 _ B _ seq _ 9 , 17 , 37 , 39 , 43 - 44 , 48 , 50 , 53 - 58 , 66 , 77 - 81, 86 , 88 , 90 - 93 , listing . txt" , which is 138 , 581, 829 bytes (measured in MS 97 , 106 , 108 - 109 , 118 - 119 , 147 , 167 , 169 - 180 , 195 , 211, WINDOWS ) which was electronically filed on a CD - ROM 225 , 234 , or 237 -238 or homologous genes. When the which was created on Sep . 28 , 2009 is incorporated herein recombinant DNA construct is expressed in corn plants , the by reference . endogenous protein is a corn protein with an amino acid sequence of SEQ ID NO :316 , 344 , 346 , 350 - 351 , 355 , 357 , FIELD OF THE INVENTION 360 - 365 , 384 - 388 , 393 , 397 -400 , 404, 413 , 415 -416 , 425 [0003 ] Disclosed herein are recombinant DNA useful for 426 , 474 , 476 - 487 , 502 , 532 , 541 , or 544 - 545 or the corn providing enhanced traits to transgenic plants , seeds, pollen , homolog of SEQ ID NOs: 309 , 311 , 324 , 373 , 395 , 454 , or plant cells and plant nuclei of such transgenic plants , meth 518 ; when the recombinant DNA construct is expressed in ods of making and using such recombinant DNA , plants , soybean plants , the endogenous protein is a soybean protein seeds , pollen , plant cells and plant nuclei. Also disclosed are with an amino acid sequence of SEQ ID NO : 309 , 324 , 373 , methods of producing hybrid corn seed comprising such 395 , 518 or is a soybean homolog of SEQ ID NOs: 311 , 316 , recombinant DNA . 344 , 346 , 350 -351 , 355 , 357, 360 -365 , 384 - 388 , 393 , 397 [ 0004 ] All genetic resources disclosed herein were 400 , 404 , 413 , 415 - 416 , 425 - 426 , 454 , 474, 476 - 487 , 502 , directly obtained from sources that are currently common to 532 , 541 , or 544 - 545 ; and when the recombinant DNA the United States ; the ancestral sources of each specific construct is expressed in a plant other than a corn or a genetic material is unknown . soybean plant, the endogenous protein is the other plant' s endogenous protein that has an amino acid sequence SUMMARY OF THE INVENTION homologous to SEQ ID NO : 309 , 311 , 316 , 324 , 344 , 346 , [0005 ] An aspect of this invention provides recombinant 350 - 351 , 355 , 357 , 360 - 365 , 373 , 384 - 388 , 393 , 395 , 397 DNA constructs comprising polynucleotides characterized 400 , 404 , 413 , 415 -416 , 425 -426 , 454 , 474 , 476 - 487 , 502 , by an encoded protein having amino acids representing a 518 , 532 , 541, or 544 - 545 . protein family domain module as described in Table 10 . 100071 In practical aspects of this invention the recombi Another aspect of this invention provides recombinant DNA nant DNA constructs of the invention are stably integrated constructs comprising polynucleotides characterized by an into the chromosome of a plant cell nucleus. encoded protein with an amino acid sequence that is at least [ 0008 ] This invention also provides transgenic plant cells 9 ) % identical to a corresponding consensus sequence comprising the stably integrated recombinant DNA con defined in table 8 . Yet another aspect of this invention structs of the invention , transgenic plants and seeds com provides recombinant DNA constructs comprising poly prising a plurality of such transgenic plant cells and trans nucleotides characterized by reference to SEQ ID NO : 1 - 307 genic pollen of such plants. Such transgenic plants are and the cognate proteins with amino acid sequences having selected from a population of transgenic plants regenerated reference to SEQ ID NO : 308 -614 . The recombinant DNA from plant cells transformed with recombinant DNA con constructs are useful for providing enhanced traits when structs by screening transgenic plants for an enhanced trait stably integrated
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