Biosafety Manual

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Biosafety Manual U 2014 N I V E R S Biosafety I T Manual Y O F F L O R Business Affairs I D Division of Environmental Health & Safety A BioSafety Manual University of Florida Business Affairs Environmental Health and Safety Box 112190 Gainesville, Florida 32611 (352) 392-1591 phone (352) 392-3647 fax Email: [email protected] http://www.ehs.ufl.edu/programs/bio/ 2014 1 Emergency Contacts General Police Emergency: University Police Department 392-1111 Fire Emergency: Gainesville Fire Rescue 911 Medical Emergency: Gainesville Fire Rescue 911 Gas leak: Gainesville Fire Rescue 911 Spills/Releases/Accidents: Notify the Principal Investigator, Lab Director/Supervisor and as appropriate for the following items: Business Hours After Hours Asbestos 392-3392 392-1111 Biological or Recombinant Materials 392-1591 392-1111 Select Agents 392-1591 392-1111 Chemicals (laboratory) 392-1591 392-1111 Mercury 392-8400 392-1111 Pesticides 392-1904 392-1111 Radioactive Materials: Campus 392-7359 392-1111 Radioactive Materials: Health Science Center 392-1589 392-1111 Needle Stick Hotline: 1-866-477-6824 (OUCH) Notify the Principal Investigator, or Lab Director / Supervisor and during working hours employees must call the UF Worker’s Compensation Office (UFWC) at (352) 392-4940 prior to seeking treatment for work related injuries that are not immediate medical emergencies. See chapter on exposures and incidents for additional information. Student Health Care Center Clinic, D2-49: (352) 294-5700 2 Table of Contents Authority & Responsibilities ............................................................................................. 6 Policy Statement ............................................................................................................. 6 Responsibilities ............................................................................................................... 7 Biosafety: Fundamentals and Definitions ........................................................................ 7 Biohazards ................................................................................................................................................. 8 Regulatory Authority .................................................................................................................................. 8 Biohazards Risk Assessment .......................................................................................... 9 Objective .................................................................................................................................................... 9 Risk Assessments ..................................................................................................................................... 9 Human Pathogens ................................................................................................................................... 10 Animal Pathogens ................................................................................................................................... 12 Plant Pathogens and Greenhouses ........................................................................................................ 14 Select Agents .......................................................................................................................................... 15 Recombinant and Synthetic Nucleic Acids .............................................................................................. 16 Biological Toxins ...................................................................................................................................... 18 Mammalian Cell Cultures ........................................................................................................................ 19 Human Blood and Other Potentially Infectious Material .......................................................................... 19 Clinical Laboratories ................................................................................................................................ 20 Viral Vectors ............................................................................................................................................ 21 Biosecurity ..................................................................................................................... 29 Control of Biohazards .................................................................................................... 32 Laboratory Practices ................................................................................................................................ 32 Engineering Controls ............................................................................................................................... 33 Personal Protective Equipment ............................................................................................................... 36 Administrative Controls ............................................................................................................................ 37 Decontamination and Sterilization of Biologicals ........................................................... 39 Disinfectants ............................................................................................................................................ 40 Biomedical and Biological Waste ............................................................................................................ 42 Animal Carcasses .................................................................................................................................... 44 Disposal of Biological Toxins ................................................................................................................... 45 Autoclave Testing and Use...................................................................................................................... 45 Equipment Decontamination ................................................................................................................... 46 Laboratory Decontamination ................................................................................................................... 47 Emergencies ................................................................................................................. 47 Personnel Responsibilities/Assignments During a Disaster .................................................................... 47 Emergency Evacuation Procedures ........................................................................................................ 48 Fire Safety ............................................................................................................................................... 49 Break-In/Security Breach ......................................................................................................................... 49 Workplace Violence ................................................................................................................................. 50 3 Hurricane Watch ...................................................................................................................................... 50 Tornadoes and Other Natural Disasters .................................................................................................. 50 Bomb Threats .......................................................................................................................................... 50 Natural/LP Gas Leak ............................................................................................................................... 51 Suspicious Package ................................................................................................................................ 51 Explosions ............................................................................................................................................... 51 Power Outage .......................................................................................................................................... 52 Exposures and Incidents ............................................................................................... 52 Exposures ................................................................................................................................................ 52 Incidents: Spills and Injuries .................................................................................................................... 54 Occupational Medicine Programs ................................................................................. 58 Bloodborne Pathogen (BBP) Program .................................................................................................... 58 Animal Contact Medical Monitoring Program .......................................................................................... 59 Respiratory Protection Program .............................................................................................................. 60 BioPath Medical Monitoring Program ...................................................................................................... 61 Vaccination Policy for Research Personnel ............................................................................................ 62 Packaging, Shipping and Transport of Biological Materials ........................................... 64 Permits .........................................................................................................................
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