Root Canal Morphology of Permanent Teeth in a Malaysian Subpopulation
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Pan et al. BMC Oral Health (2019) 19:14 https://doi.org/10.1186/s12903-019-0710-z RESEARCHARTICLE Open Access Root canal morphology of permanent teeth in a Malaysian subpopulation using cone- beam computed tomography Julia Yen Yee Pan1, Abhishek Parolia2,5*, Siong Ren Chuah1, Shekhar Bhatia2, Sunil Mutalik3 and Allan Pau4 Abstract Background: To determine the root canal morphology of human permanent maxillary and mandibular teeth in a Malaysian subpopulation using cone-beam computed tomography (CBCT). Methods: A total of 208 CBCT images were examined retrospectively. Prevalence of an extra root/canal and internal morphology based on Vertucci’s classification were observed in human maxillary and mandibular permanent teeth. Variations in the external and internal morphology were compared in relation to gender and tooth side (left vs right) using Pearson Chi-square and Fisher’s exact tests with significance level set at p < 0.05. Results: In the maxillary arch, the prevalence of three canals were observed in 0.3% of first premolars and two canals in 46.5% of second premolars. Males displayed significantly higher prevalence of two canals in maxillary second premolars than females (p < 0.05). The prevalence of a second mesiobuccal canal in maxillary first and second molars were 36.3 and 8.5%, respectively. Males displayed significantly higher prevalence of a second mesiobuccal canal in maxillary second molars than females (p < 0.05). The prevalence of a second palatal canal in maxillary first and second molars were 0.9 and 0.6%, respectively. In the mandibular arch, the prevalence of two canals were observed in 5.1% of central incisors, 12.3% of lateral incisors, 6.1% of canines, 18.7% of first premolars and 0.5% of second premolars. The prevalence of a middle mesial canal, second distal canal and extra root (radix entomolaris) were detected in 1.9, 19.5 and 21.4% of mandibular first molars, respectively. The prevalence of a C-shaped canal was observed in 48.7% of mandibular second molars. Females displayed significantly higher prevalence of a C-shaped canal in the right mandibular second molars than males (p < 0.05). No other statistically significant differences in root anatomy and root canal morphology were observed in relation to gender and tooth side. Conclusions: Wide variations in the root canal morphology exist among Malaysians. CBCT is a clinically useful tool in the identification of external and internal morphological variations in the human teeth. Keywords: Cone-beam computed tomography, Malaysian, Morphology, Root canal Background a major endodontic challenge to clinicians. Therefore, The success of an endodontic treatment requires a thorough knowledge and understanding of their comprehensive knowledge of the root anatomy and root anatomy and variations is of utmost importance to avoid canal morphology along with its variations within the potential complications and undesirable failures during norm. In dental practice, the presence of anatomical endodontic procedures [1]. aberrations in the root and root canal system may pose Globally, there are numerous studies [2–7] reporting the internal and external morphological variations among different ethnicities. Only a few studies [8–11] * Correspondence: [email protected] had been conducted to evaluate the root canal morph- 2Division of Clinical Dentistry, School of Dentistry, International Medical University (IMU), Bukit Jalil, Kuala Lumpur, Malaysia ology in a Malaysian population. 5Bukit Jalil, Kuala Lumpur, Malaysia Full list of author information is available at the end of the article © The Author(s). 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. Pan et al. BMC Oral Health (2019) 19:14 Page 2 of 15 Abd Latib et al. [8] reported the prevalence of a sec- Inclusion and exclusion criteria ond mesiobuccal canal in maxillary first molars among Healthy, untreated, fully developed, permanent maxillary Malaysians was 18% for males and 10% for females. Choi and mandibular incisors, canines, first and second pre- et al. [9] compared the incidence of a second mesiobuc- molars and molars in individuals were included. Cases cal canal in maxillary first molars between Caucasians whereby anatomy was compromised by physiological or and Malaysians. In that study, 65.44% of Malaysians pathological processes and the original root anatomy were found to have two mesiobuccal canals [9]. Bhatia et was not clear, were excluded. al. [10] reported the prevalence of radix molars in ex- tracted first molars among Malaysians was 4.9% whereby Pilot study the prevalence of radix entomolaris (4.2%) was more Prior to the start of study, the researcher underwent train- common than radix paramolaris (0.7%). Besides that, an ing from trained oral radiologist and endodontists on how in vitro study by Nie et al. [11] reported that the occur- to operate the CBCT and identify root canal morphology rence of C-shaped canal in mandibular second molars from CBCT images, respectively. The pilot study was car- among Malaysians was 8 (3.3%) out of 241 cases or ried out using 20 CBCT images whereby all observations 88.8% of total number of mandibular molars. In that were performed independently by the researcher, oral study, C-shaped canal was found to be more common in radiologist and endodontists. CBCT images were studied the Chinese race in which it was proven to be statisti- using the same computer and screen under ambient room cally significant [11]. lighting conditions. Inter-observer reliability test was car- Various methodologies have been employed to evaluate ried out using Cohen’s Kappa test (< 0 less than chance the root and root canal morphology such as the use of agreement, 0.01–0.20 slight agreement, 0.21–0.40 fair clearing technique, cross-sectioning technique, analysis of agreement, 0.41–0.60 moderate agreement, 0.61–0.80 extracted teeth, conventional radiography and cone-beam substantial agreement, and 0.81–0.99 almost perfect computed tomography (CBCT). With the advent of agreement) as proposed by Viera & Garrett [13]. A final CBCT, many recent endodontic studies have been incorp- consensus was reached when the inter-rater agreement orating the three-dimensional approach as CBCT offers a kappa value was found to be within the substantial agree- wide array of advantages. CBCT permits modification of ment (0.61–0.80) or almost perfect agreement (0.81–099). the visual field, yields a high resolution and produces a Throughout the study, the calibration of the researcher minimal amount of radiation as compared to conventional was tested by repeating the observations twice on a ran- computed tomography [12]. Furthermore, majority of the dom sample of 50 CBCT images. CBCT equipment are ergonomically designed for safe use and optimal performance [12]. Radiographic technique and image analysis The present study therefore aimed to investigate the The images for the study were selected from the data- prevalence of morphological variations in the root anat- base of KaVo 3D eXam imaging system (Imaging Sci- omy and root canal morphology of human maxillary and ences International, Hatfield, PA, USA). The images mandibular permanent teeth in a Malaysian subpopula- were acquired from a standard protocol for patient posi- tion using CBCT and to correlate the findings with gen- tioning, exposure parameter setting (120 kVp, 5 mA, der and tooth side. 26.9 s) and image acquisition of 0.25 mm voxel size. The images were analysed on eXam Vision software version Methods 1.9.3.13 (KaVo Dental GmbH, Biberach, Germany). The Sample selection axial, coronal and sagittal planes, as well as custom The present study consisted of 208 CBCT images of max- planes of the selected images were magnified to 150% to illary and mandibular permanent teeth that had been visualise the cross section of root canal morphology. taken from patients who visited the Oral Health Centre at International Medical University (IMU) for diagnosis and Measurements preoperative assessment for implants, surgical extraction The variables evaluated were patient’s gender, age, ethnicity, and orthodontic treatment. Written informed consents tooth side, number of roots and root canal configuration. were obtained from patients whose images were used. The canal configurations were categorized into the follow- Based on the previous studies [8–11] on Malaysian popu- ing eight types based on Vertucci’s classification [14]: lation, an expected frequency of 15%, an acceptable mar- gin of error of 5% and a design effect set at 1 with 95% 1. Type I: A single canal extends from the pulp confidence interval were used as parameters for Epi Info chamber to the apex. StatCalc statistical software (version 7.2.0.1) to calculate 2. Type II: Two separate canals leave the pulp the total sample size. Epi Info is a trademark of the Cen- chamber and join short of the apex to form one ters for Disease Control and Prevention. canal. Pan et al. BMC Oral Health (2019) 19:14 Page 3 of 15 3. Type III: One canal leaves the pulp chamber and Maxillary teeth divides into two within the root, and then merge to The distribution of the root canal morphology of maxil- exit as one canal. lary teeth based on Vertucci’s classification are shown in 4. Type IV: Two separate and distinct canals extend Table 1. Based on Fig. 1(a-g), Type I, II, III, IV, V, VI and from the pulp chamber to the apex.