DOCSLIB.ORG

Understanding Biological Factors Associated with Pelvic Organ Prolapse in Late Gestation Sows

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Understanding Biological Factors Associated with Pelvic Organ Prolapse in Late Gestation Sows Iowa State University Capstones, Theses and Graduate Theses and Dissertations Dissertations 2021 Understanding biological factors associated with pelvic organ prolapse in late gestation sows Zoe E. Kiefer Iowa State University Follow this and additional works at: https://lib.dr.iastate.edu/etd Recommended Citation Kiefer, Zoe E., "Understanding biological factors associated with pelvic organ prolapse in late gestation sows" (2021). Graduate Theses and Dissertations. 18526. https://lib.dr.iastate.edu/etd/18526 This Thesis is brought to you for free and open access by the Iowa State University Capstones, Theses and Dissertations at Iowa State University Digital Repository. It has been accepted for inclusion in Graduate Theses and Dissertations by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. Understanding biological factors associated with pelvic organ prolapse in late gestation sows by Zoë Elizabeth Kiefer A thesis submitted to the graduate faculty in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE Major: Animal Physiology (Reproductive Physiology) Program of Study Committee: Jason W. Ross, Major Professor Aileen F. Keating Stephan Schmitz-Esser The student author, whose presentation of the scholarship herein was approved by the program of study committee, is solely responsible for the content of this thesis. The Graduate College will ensure this thesis is globally accessible and will not permit alterations after a degree is conferred. Iowa State University Ames, Iowa 2021 Copyright © Zoë Elizabeth Kiefer, 2021. All rights reserved. ii DEDICATION I dedicate this thesis to everyone who has encouraged and supported me throughout this journey. To my family, friends, colleagues and mentors, thank you for helping me become the scientist I am today. To the Iowa State University Department of Animal Science thanks for a great experience and excellent education. LOYAL ● FOREVER ● TRUE iii TABLE OF CONTENTS Page LIST OF FIGURES .........................................................................................................................v LIST OF TABLES ......................................................................................................................... vi NOMENCLATURE ..................................................................................................................... vii ACKNOWLEDGMENTS ............................................................................................................. ix ABSTRACT .....................................................................................................................................x CHAPTER 1. INTRODUCTION ....................................................................................................1 References ................................................................................................................................. 2 CHAPTER 2. LITERATURE REVIEW .........................................................................................4 Sow Reproduction ..................................................................................................................... 4 Onset of Puberty ................................................................................................................... 4 Estrous Cycle ........................................................................................................................ 5 Gilt Development ................................................................................................................. 6 Sow Mortality in the U.S. Swine Industry ................................................................................. 7 Reproductive Tract Dysfunction Overview ............................................................................... 7 Reproductive Disease in Humans......................................................................................... 8 Reproductive Disease in Cattle ............................................................................................ 9 Physiology of Pelvic Organ Prolapse ................................................................................. 10 Vaginal Microbiota Overview ................................................................................................. 11 Vaginal Microbiota of Humans .......................................................................................... 12 Vaginal Microbiota of Cattle .............................................................................................. 14 Vaginal Microbiota of Swine ............................................................................................. 15 Role of the Vaginal Microbiota in the Immune System ..................................................... 16 Immune and Inflammatory Response ...................................................................................... 17 Steroid Hormone Changes During Late Gestation .................................................................. 20 Steroidogenesis ................................................................................................................... 20 Steroid Hormone Effects on the Immune Response .......................................................... 21 Steroid Hormones and their Potential Influence on the Microbiota ................................... 22 Summary .................................................................................................................................. 23 References ............................................................................................................................... 24 CHAPTER 3. VAGINAL MICROBIOME AND SERUM METABOLITE DIFFERENCES IN LATE GESTATION COMMERCIAL SOWS AT RISK FOR PELVIC ORGAN PROLAPSE .33 Abstract .................................................................................................................................... 33 Introduction ............................................................................................................................. 34 Results ..................................................................................................................................... 35 Discussion ................................................................................................................................ 38 Materials and Methods ............................................................................................................ 43 iv Statistical analyses ................................................................................................................... 47 Data availability ....................................................................................................................... 49 Acknowledgements ................................................................................................................. 49 Conflict of Interest ................................................................................................................... 50 Authors contributions .............................................................................................................. 50 References ............................................................................................................................... 50 CHAPTER 4. VAGINAL MICROBIOTA VARIATION DURING LATE GESTATION COMMERCIAL SOWS A RISK FOR PELVIC ORGAN PROLAPSE ......................................63 Abstract .................................................................................................................................... 63 Introduction ............................................................................................................................. 64 Materials and Methods ............................................................................................................ 66 Results ..................................................................................................................................... 71 Discussion ................................................................................................................................ 75 Acknowledgements ................................................................................................................. 81 Conflict of Interest ................................................................................................................... 81 Authors contributions .............................................................................................................. 81 References ............................................................................................................................... 82 CHAPTER 5. CIRCULATING BIOMARKERS ASSOCIATED WITH PELVIC ORGAN PROLASPE RISK IN LATE GESATION SOWS ........................................................................98 Abstract .................................................................................................................................... 98 Introduction ............................................................................................................................. 99 Materials and Methods .......................................................................................................... 101 Results ................................................................................................................................... 104 Discussion
Load more

Recommended publications
  • A New Emergent Risk Factor for Endometrial Cancer
    Journal of Personalized Medicine Review Gut and Endometrial Microbiome Dysbiosis: A New Emergent Risk Factor for Endometrial Cancer Soukaina Boutriq 1,2,3, Alicia González-González 1,2 , Isaac Plaza-Andrades 1,2, Aurora Laborda-Illanes 1,2,3, Lidia Sánchez-Alcoholado 1,2,3, Jesús Peralta-Linero 1,2, María Emilia Domínguez-Recio 1, María José Bermejo-Pérez 1, Rocío Lavado-Valenzuela 2,*, Emilio Alba 1,4,* and María Isabel Queipo-Ortuño 1,2,4 1 Unidad de Gestión Clínica Intercentros de Oncología Médica, Hospitales Universitarios Regional y Virgen de la Victoria, Instituto de Investigación Biomédica de Málaga (IBIMA)-CIMES-UMA, 29010 Málaga, Spain; [email protected] (S.B.); [email protected] (A.G.-G.); [email protected] (I.P.-A.); [email protected] (A.L.-I.); [email protected] (L.S.-A.); [email protected] (J.P.-L.); [email protected] (M.E.D.-R.); [email protected] (M.J.B.-P.); [email protected] (M.I.Q.-O.) 2 Instituto de Investigación Biomédica de Málaga (IBIMA), Campus de Teatinos s/n, 29071 Málaga, Spain 3 Facultad de Medicina, Universidad de Málaga, 29071 Málaga, Spain 4 Centro de Investigación Biomédica en Red de Cáncer (Ciberonc CB16/12/00481), 28029 Madrid, Spain * Correspondence: [email protected] (R.L.-V.); [email protected] (E.A.) Abstract: Endometrial cancer is one of the most common gynaecological malignancies worldwide. Histologically, two types of endometrial cancer with morphological and molecular differences and Citation: Boutriq, S.; also therapeutic implications have been identified. Type I endometrial cancer has an endometrioid González-González, A.; morphology and is estrogen-dependent, while Type II appears with non-endometrioid differentiation Plaza-Andrades, I.; Laborda-Illanes, and follows an estrogen-unrelated pathway.
    [Show full text]
  • Ecology and Epidemiology of Campylobacter Jejuni in Broiler Chickens
    Ecology and Epidemiology of Campylobacter jejuni in Broiler Chickens A DISSERTATION SUBMITTED TO THE FACULTY OF THE UNIVERSITY OF MINNESOTA BY Hae Jin Hwang IN PARTIALL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY Dr. Randall Singer, Dr. George Maldonado June 2019 © Hae Jin Hwang, 2019 Acknowledgements I would like to sincerely thank my advisor, Dr. Randall Singer, for his intellectual guidance and support, great patience, and mentorship, which made this dissertation possible. I would also like to thank Dr. George Maldonado for his continuous encouragement and support. I would further like to thank my thesis committee, Dr. Richard Isaacson and Dr. Timothy Church, for their guidance throughout my doctoral training. I thank all my friends and colleagues I met over the course of my studies. I am especially indebted to my friends, Dr. Kristy Lee, Dr. Irene Bueno Padilla, Dr. Elise Lamont, Madhumathi Thiruvengadam, Dr. Kaushi Kanankege and Dr. Sylvia Wanzala, for their support and friendship. Heartfelt gratitude goes to my family, for always believing in me, encouraging me and helping me get through the difficult and stressful times during my studies. Lastly, I thank Sven and Bami for being the best writing companions I could ever ask for. i Abstract Campylobacteriosis, predominantly caused by Campylobacter jejuni, is a common, yet serious foodborne illness. With consumption and handling of poultry products as the most important risk factor of campylobacteriosis, reducing Campylobacter contamination in poultry products is considered the best public health intervention to reduce the burden and costs associated with campylobacteriosis. To this end, there is a need to improve our understanding of epidemiology and ecology of Campylobacter jejuni in poultry.
    [Show full text]
  • 1 Aix-Marseille Universite Faculte De Médecine De
    AIX-MARSEILLE UNIVERSITE FACULTE DE MÉDECINE DE MARSEILLE ECOLE DOCTORALE DES SCIENCES DE LA VIE ET DE LA SANTE T H È S E Présentée et publiquement soutenue devant LA FACULTÉ DE MÉDECINE DE MARSEILLE Le 21 Novembre 2019 Par Mr DIALLO Ousmane Oumou Né le 02 Février 1988 à Dalaba SURVEILLANCE DE LA RESISTANCE AUX ANTIBIOTIQUES DANS LA REGION PROVENCE ALPES COTE D’AZUR A PARTIR DES SYSTEMES DE SURVEILLANCE (MARSS et PACASURVE) Pour obtenir le grade de Doctorat d’Aix-Marseille Universités Spécialité Pathologie Humaine : Maladies infectieuses Membres du jury de Thèse Pr. Laurence Camoin Président du jury Pr. Jean Philippe Lavigne Rapporteur Pr. Max Maurin Rapporteur Pr. Jean-Marc Rolain Directeur de thèse MEPHI, Aix Marseille Université, IHU Méditerranée Infection, AP-HM, Marseille Faculté de Médecine, Marseille. 1 1 AVANT PROPOS Le format de présentation de cette thèse correspond à une recommandation de la spécialité Maladies Infectieuses et Microbiologie, à l’intérieur du Master des Sciences de la Vie et de la Santé qui dépend de l’École Doctorale des Sciences de la Vie de Marseille. Le candidat est amené à respecter des règles qui lui sont imposées et qui comportent un format de thèse utilisé dans le Nord de l’Europe et qui permet un meilleur rangement que les thèses traditionnelles. Par ailleurs, la partie introduction et bibliographie est remplacée par une revue envoyée dans un journal afin de permettre une évaluation extérieure de la qualité de la revue et de permettre à l’étudiant de commencer le plus tôt possible une bibliographie exhaustive sur le domaine de cette thèse.
    [Show full text]
  • A Taxonomic Note on the Genus Lactobacillus
    Taxonomic Description template 1 A taxonomic note on the genus Lactobacillus: 2 Description of 23 novel genera, emended description 3 of the genus Lactobacillus Beijerinck 1901, and union 4 of Lactobacillaceae and Leuconostocaceae 5 Jinshui Zheng1, $, Stijn Wittouck2, $, Elisa Salvetti3, $, Charles M.A.P. Franz4, Hugh M.B. Harris5, Paola 6 Mattarelli6, Paul W. O’Toole5, Bruno Pot7, Peter Vandamme8, Jens Walter9, 10, Koichi Watanabe11, 12, 7 Sander Wuyts2, Giovanna E. Felis3, #*, Michael G. Gänzle9, 13#*, Sarah Lebeer2 # 8 '© [Jinshui Zheng, Stijn Wittouck, Elisa Salvetti, Charles M.A.P. Franz, Hugh M.B. Harris, Paola 9 Mattarelli, Paul W. O’Toole, Bruno Pot, Peter Vandamme, Jens Walter, Koichi Watanabe, Sander 10 Wuyts, Giovanna E. Felis, Michael G. Gänzle, Sarah Lebeer]. 11 The definitive peer reviewed, edited version of this article is published in International Journal of 12 Systematic and Evolutionary Microbiology, https://doi.org/10.1099/ijsem.0.004107 13 1Huazhong Agricultural University, State Key Laboratory of Agricultural Microbiology, Hubei Key 14 Laboratory of Agricultural Bioinformatics, Wuhan, Hubei, P.R. China. 15 2Research Group Environmental Ecology and Applied Microbiology, Department of Bioscience 16 Engineering, University of Antwerp, Antwerp, Belgium 17 3 Dept. of Biotechnology, University of Verona, Verona, Italy 18 4 Max Rubner‐Institut, Department of Microbiology and Biotechnology, Kiel, Germany 19 5 School of Microbiology & APC Microbiome Ireland, University College Cork, Co. Cork, Ireland 20 6 University of Bologna, Dept. of Agricultural and Food Sciences, Bologna, Italy 21 7 Research Group of Industrial Microbiology and Food Biotechnology (IMDO), Vrije Universiteit 22 Brussel, Brussels, Belgium 23 8 Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Ghent, 24 Belgium 25 9 Department of Agricultural, Food & Nutritional Science, University of Alberta, Edmonton, Canada 26 10 Department of Biological Sciences, University of Alberta, Edmonton, Canada 27 11 National Taiwan University, Dept.
    [Show full text]
  • Comparative Genomics of the Genus Porphyromonas Identifies Adaptations for Heme Synthesis Within the Prevalent Canine Oral Species Porphyromonas Cangingivalis
    GBE Comparative Genomics of the Genus Porphyromonas Identifies Adaptations for Heme Synthesis within the Prevalent Canine Oral Species Porphyromonas cangingivalis Ciaran O’Flynn1,*, Oliver Deusch1, Aaron E. Darling2, Jonathan A. Eisen3,4,5, Corrin Wallis1,IanJ.Davis1,and Stephen J. Harris1 1 The WALTHAM Centre for Pet Nutrition, Waltham-on-the-Wolds, United Kingdom Downloaded from 2The ithree Institute, University of Technology Sydney, Ultimo, New South Wales, Australia 3Department of Evolution and Ecology, University of California, Davis 4Department of Medical Microbiology and Immunology, University of California, Davis 5UC Davis Genome Center, University of California, Davis http://gbe.oxfordjournals.org/ *Corresponding author: E-mail: ciaran.ofl[email protected]. Accepted: November 6, 2015 Abstract Porphyromonads play an important role in human periodontal disease and recently have been shown to be highly prevalent in canine mouths. Porphyromonas cangingivalis is the most prevalent canine oral bacterial species in both plaque from healthy gingiva and at University of Technology, Sydney on January 17, 2016 plaque from dogs with early periodontitis. The ability of P. cangingivalis to flourish in the different environmental conditions char- acterized by these two states suggests a degree of metabolic flexibility. To characterize the genes responsible for this, the genomes of 32 isolates (including 18 newly sequenced and assembled) from 18 Porphyromonad species from dogs, humans, and other mammals were compared. Phylogenetic trees inferred using core genes largely matched previous findings; however, comparative genomic analysis identified several genes and pathways relating to heme synthesis that were present in P. cangingivalis but not in other Porphyromonads. Porphyromonas cangingivalis has a complete protoporphyrin IX synthesis pathway potentially allowing it to syn- thesize its own heme unlike pathogenic Porphyromonads such as Porphyromonas gingivalis that acquire heme predominantly from blood.
    [Show full text]
  • Metatranscriptomic Analysis Reveals Active Bacterial Communities in Diabetic Foot Infections
    fmicb-11-01688 July 20, 2020 Time: 12:22 # 1 ORIGINAL RESEARCH published: 22 July 2020 doi: 10.3389/fmicb.2020.01688 Metatranscriptomic Analysis Reveals Active Bacterial Communities in Diabetic Foot Infections Fatemah Sadeghpour Heravi1, Martha Zakrzewski2, Karen Vickery1, Matthew Malone3,4,5 and Honghua Hu1* 1 Surgical Infection Research Group, Faculty of Medicine and Health Sciences, Macquarie University, Sydney, NSW, Australia, 2 QIMR Berghofer Medical Research Institute, Brisbane, QLD, Australia, 3 Infectious Diseases and Microbiology, School of Medicine, Western Sydney University, Sydney, NSW, Australia, 4 Liverpool Hospital, South Western Sydney LHD, Sydney, NSW, Australia, 5 Liverpool Diabetes Collaborative Research Unit, Ingham Institute for Applied Medical Research, Sydney, NSW, Australia Despite the extended view of the composition of diabetic foot infections (DFIs), little is known about which transcriptionally active bacterial communities are pertinent to infection, and if any differences are associated with increased infection severity. We applied a RNA sequencing approach to analyze the composition, function, and pathogenicity of the active bacterial communities in DFIs. Taxonomic profiling of bacterial transcripts revealed the presence of 14 bacterial phyla in DFIs. The abundance Edited by: Qi Zhao, of the Spiroplasma, Vibrio, and Mycoplasma were significantly different in different Liaoning University, China infection severities (P < 0.05). Mild and severe stages of infections were dominated Reviewed by: by Staphylococcus aureus and Porphyromonas asaccharolytica, respectively. A total of Patrick R. M. Harnarayan, The University of the West Indies 132 metabolic pathways were identified of which ribosome and thiamin being among the at St. Augustine, Trinidad and Tobago most highly transcribed pathways. Moreover, a total of 131 antibiotic resistance genes, Maria José Saavedra, primarily involved in the multidrug efflux pumps/exporters, were identified.
    [Show full text]
  • Extensive Microbial Diversity Within the Chicken Gut Microbiome Revealed by Metagenomics and Culture
    Extensive microbial diversity within the chicken gut microbiome revealed by metagenomics and culture Rachel Gilroy1, Anuradha Ravi1, Maria Getino2, Isabella Pursley2, Daniel L. Horton2, Nabil-Fareed Alikhan1, Dave Baker1, Karim Gharbi3, Neil Hall3,4, Mick Watson5, Evelien M. Adriaenssens1, Ebenezer Foster-Nyarko1, Sheikh Jarju6, Arss Secka7, Martin Antonio6, Aharon Oren8, Roy R. Chaudhuri9, Roberto La Ragione2, Falk Hildebrand1,3 and Mark J. Pallen1,2,4 1 Quadram Institute Bioscience, Norwich, UK 2 School of Veterinary Medicine, University of Surrey, Guildford, UK 3 Earlham Institute, Norwich Research Park, Norwich, UK 4 University of East Anglia, Norwich, UK 5 Roslin Institute, University of Edinburgh, Edinburgh, UK 6 Medical Research Council Unit The Gambia at the London School of Hygiene and Tropical Medicine, Atlantic Boulevard, Banjul, The Gambia 7 West Africa Livestock Innovation Centre, Banjul, The Gambia 8 Department of Plant and Environmental Sciences, The Alexander Silberman Institute of Life Sciences, Edmond J. Safra Campus, Hebrew University of Jerusalem, Jerusalem, Israel 9 Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, UK ABSTRACT Background: The chicken is the most abundant food animal in the world. However, despite its importance, the chicken gut microbiome remains largely undefined. Here, we exploit culture-independent and culture-dependent approaches to reveal extensive taxonomic diversity within this complex microbial community. Results: We performed metagenomic sequencing of fifty chicken faecal samples from Submitted 4 December 2020 two breeds and analysed these, alongside all (n = 582) relevant publicly available Accepted 22 January 2021 chicken metagenomes, to cluster over 20 million non-redundant genes and to Published 6 April 2021 construct over 5,500 metagenome-assembled bacterial genomes.
    [Show full text]
  • Postmenopause As a Key Factor in the Composition of the Endometrial Cancer Microbiome (Ecbiome) Dana M
    www.nature.com/scientificreports OPEN Postmenopause as a key factor in the composition of the Endometrial Cancer Microbiome (ECbiome) Dana M. Walsh 1,2,8, Alexis N. Hokenstad3,8, Jun Chen1,4, Jaeyun Sung1,2,5,6, Gregory D. Jenkins4, Nicholas Chia1,2, Heidi Nelson1,2, Andrea Mariani7* & Marina R. S. Walther-Antonio1,2,3* Incidence rates for endometrial cancer (EC) are rising, particularly in postmenopausal and obese women. Previously, we showed that the uterine and vaginal microbiome distinguishes patients with EC from those without. Here, we sought to examine the impact of patient factors (such as menopause status, body mass index, and vaginal pH) in the microbiome in the absence of EC and how these might contribute to the microbiome signature in EC. We fnd that each factor independently alters the microbiome and identifed postmenopausal status as the main driver of a polymicrobial network associated with EC (ECbiome). We identifed Porphyromas somerae presence as the most predictive microbial marker of EC and we confrm this using targeted qPCR, which could be of use in detecting EC in high-risk, asymptomatic women. Given the established pathogenic behavior of P. somerae and accompanying network in tissue infections and ulcers, future investigation into their role in EC is warranted. Endometrial cancer (EC) is the most common gynecological malignancy in the United States and the fourth most common cancer among women1,2. In addition, EC incidence rates are on the rise in the western world, suggesting that alterations in environmental factors such as diet, lifestyle, and the microbiome may be important drivers in EC etiology3,4.
    [Show full text]
  • Demonstrating the Potential of Abiotic Stress-Tolerant Jeotgalicoccus Huakuii NBRI 13E for Plant Growth Promotion and Salt Stress Amelioration
    Annals of Microbiology (2019) 69:419–434 https://doi.org/10.1007/s13213-018-1428-x ORIGINAL ARTICLE Demonstrating the potential of abiotic stress-tolerant Jeotgalicoccus huakuii NBRI 13E for plant growth promotion and salt stress amelioration Sankalp Misra1,2 & Vijay Kant Dixit 1 & Shashank Kumar Mishra1,2 & Puneet Singh Chauhan1,2 Received: 10 September 2018 /Accepted: 20 December 2018 /Published online: 2 January 2019 # Università degli studi di Milano 2019 Abstract The present study aimed to demonstrate the potential of abiotic stress-tolerant Jeotgalicoccus huakuii NBRI 13E for plant growth promotion and salt stress amelioration. NBRI 13E was characterized for abiotic stress tolerance and plant growth-promoting (PGP) attributes under normal and salt stress conditions. Phylogenetic comparison of NBRI 13E was carried out with known species of the same genera based on 16S rRNA gene. Plant growth promotion and rhizosphere colonization studies were determined under greenhouse conditions using maize, tomato, and okra. Field experiment was also performed to assess the ability of NBRI 13E inoculation for improving growth and yield of maize crop in alkaline soil. NBRI 13E demonstrated abiotic stress tolerance and different PGP attributes under in vitro conditions. Phylogenetic and differential physiological analysis revealed considerable differences in NBRI 13E as compared with the reported species for Jeotgalicoccus genus. NBRI 13E colonizes in the rhizosphere of the tested crops, enhances plant growth, and ameliorates salt stress in a greenhouse experiment. Modulation in defense enzymes, chlorophyll, proline, and soluble sugar content in NBRI 13E-inoculated plants leads to mitigate the deleterious effect of salt stress. Furthermore, field evaluation of NBRI 13E inoculation using maize was carried out with recommended 50 and 100% chemical fertilizer controls, which resulted in significant enhancement of all vegetative parameters and total yield as compared to respective controls.
    [Show full text]
  • Intraspecies Comparative Genomics of Rickettsia
    AIX ͲMARSEILLE UNIVERSITÉ FACULTÉ DE MÉDECINE DE MARSEILLE ÉCOLE DOCTORALE DES SCIENCES DE LA VIE ET DE LA SANTÉ T H È S E Présentée et publiquement soutenue devant LA FACULTÉ DE MÉDECINE DE MARSEILLE Le 13 décembre 2013 Par M. Erwin SENTAUSA Né le 16 décembre 1979 àMalang, Indonésie INTRASPECIES COMPARATIVE GENOMICS OF RICKETTSIA Pour obtenir le grade de DOCTORAT d’AIX ͲMARSEILLE UNIVERSITÉ SPÉCIALITÉ :PATHOLOGIE HUMAINE Ͳ MALADIES INFECTIEUSES Membres du Jury de la Thèse : Dr. Patricia RENESTO Rapporteur Pr. Max MAURIN Rapporteur Dr. Florence FENOLLAR Membre du Jury Pr. Pierre ͲEdouard FOURNIER Directeur de thèse Unité de Recherche sur les Maladies Infectieuses et Tropicales Émergentes UM63, CNRS 7278, IRD 198, Inserm 1095 Avant Propos Le format de présentation de cette thèse correspond à une recommandation de la spécialité Maladies Infectieuses et Microbiologie, à l’intérieur du Master de Sciences de la Vie et de la Santé qui dépend de l’Ecole Doctorale des Sciences de la Vie de Marseille. Le candidat est amené àrespecter des règles qui lui sont imposées et qui comportent un format de thèse utilisé dans le Nord de l’Europe permettant un meilleur rangement que les thèses traditionnelles. Par ailleurs, la partie introduction et bibliographie est remplacée par une revue envoyée dans un journal afin de permettre une évaluation extérieure de la qualité de la revue et de permettre àl’étudiant de le commencer le plus tôt possible une bibliographie exhaustive sur le domaine de cette thèse. Par ailleurs, la thèse est présentée sur article publié, accepté ou soumis associé d’un bref commentaire donnant le sens général du travail.
    [Show full text]
  • Data of Read Analyses for All 20 Fecal Samples of the Egyptian Mongoose
    Supplementary Table S1 – Data of read analyses for all 20 fecal samples of the Egyptian mongoose Number of Good's No-target Chimeric reads ID at ID Total reads Low-quality amplicons Min length Average length Max length Valid reads coverage of amplicons amplicons the species library (%) level 383 2083 33 0 281 1302 1407.0 1442 1769 1722 99.72 466 2373 50 1 212 1310 1409.2 1478 2110 1882 99.53 467 1856 53 3 187 1308 1404.2 1453 1613 1555 99.19 516 2397 36 0 147 1316 1412.2 1476 2214 2161 99.10 460 2657 297 0 246 1302 1416.4 1485 2114 1169 98.77 463 2023 34 0 189 1339 1411.4 1561 1800 1677 99.44 471 2290 41 0 359 1325 1430.1 1490 1890 1833 97.57 502 2565 31 0 227 1315 1411.4 1481 2307 2240 99.31 509 2664 62 0 325 1316 1414.5 1463 2277 2073 99.56 674 2130 34 0 197 1311 1436.3 1463 1899 1095 99.21 396 2246 38 0 106 1332 1407.0 1462 2102 1953 99.05 399 2317 45 1 47 1323 1420.0 1465 2224 2120 98.65 462 2349 47 0 394 1312 1417.5 1478 1908 1794 99.27 501 2246 22 0 253 1328 1442.9 1491 1971 1949 99.04 519 2062 51 0 297 1323 1414.5 1534 1714 1632 99.71 636 2402 35 0 100 1313 1409.7 1478 2267 2206 99.07 388 2454 78 1 78 1326 1406.6 1464 2297 1929 99.26 504 2312 29 0 284 1335 1409.3 1446 1999 1945 99.60 505 2702 45 0 48 1331 1415.2 1475 2609 2497 99.46 508 2380 30 1 210 1329 1436.5 1478 2139 2133 99.02 1 Supplementary Table S2 – PERMANOVA test results of the microbial community of Egyptian mongoose comparison between female and male and between non-adult and adult.
    [Show full text]
  • ( 12 ) United States Patent
    US009956282B2 (12 ) United States Patent ( 10 ) Patent No. : US 9 ,956 , 282 B2 Cook et al. (45 ) Date of Patent: May 1 , 2018 ( 54 ) BACTERIAL COMPOSITIONS AND (58 ) Field of Classification Search METHODS OF USE THEREOF FOR None TREATMENT OF IMMUNE SYSTEM See application file for complete search history . DISORDERS ( 56 ) References Cited (71 ) Applicant : Seres Therapeutics , Inc. , Cambridge , U . S . PATENT DOCUMENTS MA (US ) 3 ,009 , 864 A 11 / 1961 Gordon - Aldterton et al . 3 , 228 , 838 A 1 / 1966 Rinfret (72 ) Inventors : David N . Cook , Brooklyn , NY (US ) ; 3 ,608 ,030 A 11/ 1971 Grant David Arthur Berry , Brookline, MA 4 ,077 , 227 A 3 / 1978 Larson 4 ,205 , 132 A 5 / 1980 Sandine (US ) ; Geoffrey von Maltzahn , Boston , 4 ,655 , 047 A 4 / 1987 Temple MA (US ) ; Matthew R . Henn , 4 ,689 ,226 A 8 / 1987 Nurmi Somerville , MA (US ) ; Han Zhang , 4 ,839 , 281 A 6 / 1989 Gorbach et al. Oakton , VA (US ); Brian Goodman , 5 , 196 , 205 A 3 / 1993 Borody 5 , 425 , 951 A 6 / 1995 Goodrich Boston , MA (US ) 5 ,436 , 002 A 7 / 1995 Payne 5 ,443 , 826 A 8 / 1995 Borody ( 73 ) Assignee : Seres Therapeutics , Inc. , Cambridge , 5 ,599 ,795 A 2 / 1997 McCann 5 . 648 , 206 A 7 / 1997 Goodrich MA (US ) 5 , 951 , 977 A 9 / 1999 Nisbet et al. 5 , 965 , 128 A 10 / 1999 Doyle et al. ( * ) Notice : Subject to any disclaimer , the term of this 6 ,589 , 771 B1 7 /2003 Marshall patent is extended or adjusted under 35 6 , 645 , 530 B1 . 11 /2003 Borody U .
    [Show full text]