PHENOL RED DEXTROSE BROTH Product Profile Product Number P9226 Appearance Pink colored, homogeneous, free flowing powder. Product Description Phenol Red Dextrose Broth is used for the Color and Clarity Red colored, clear solution determination of fermentation of dextrose in the without any precipitate. differentiation of microorganisms. The ability of an organism to ferment a specific carbohydrate in the Cultural Response Cultural characteristics are basal medium, results in the production of acid and observed after 18-24 hours at gas, which helps in the differentiation between the 35-37°C. genera and species of bacteria. Phenol Red Dextrose Broth is a complete medium with dextrose. Proteose peptone and beef extract provide nitrogenous nutrients Organisms (ATCC) Growth Acid Gas to the organisms. Phenol red is the pH indicator, which Citrobacter freundii luxuriant + + turns yellow at acidic pH. Sodium chloride maintains (8090) osmotic equilibrium. Gas formation is seen in Enterobacter aerogenes luxuriant + + Durham's tubes. (13048) Escherichia coli (25922) luxuriant + + Components Klebsiella pneumoniae luxuriant + + Item g/L (13883) Proteose Peptone 10.00 Proteus vulgaris (13315) luxuriant + + Beef Extract 1.00 Salmonella typhimurium luxuriant + + Sodium Chloride 5.00 (14028) Phenol Red 0.018 Salmonella typhi (6539) luxuriant + - Dextrose 5.00 Serratia marcescens luxuriant + + (8100) ° ± Final pH (at 25 C) 7.4 0.2 Shigella flexneri (12022) luxuriant + -

Precautions and Disclaimer References For laboratory use only. Not for drug, household or 1. American Type Culture Collection, Manassas, Va., other uses. U.S.A.

2. MacFaddin, J., 1985. Media for Isolation- Preparation Instructions Cultivation-Identification-Maintenance of Medical Suspend 21 grams of Phenol Red Dextrose Broth in Bacteria. Vol. 1. Williams and Wilkins. Baltimore, 1000 mls of distilled water. Heat if necessary to Maryland. dissolve the medium completely. Dispense into tubes 3. Finegold and Baron, 1986, Bailey and Scott’s containing.inverted Durham's tubes and sterilize by Diagnostic Microbiology, 7th ed., The C.V. Mosby ° autoclaving at 15 lbs. pressure (121 C) for 15 minutes. Co., St. Louis., U.S.A.

Storage Store the dehydrated medium at 24°C and the prepared medium at 2-8°C.

P9226 8/99

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