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Corning Cell Culture Media, Sera, Reagents, and Single-Use Technology ­Product Selection Guide

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Corning Cell Culture Media, Sera, Reagents, and Single-Use Technology ­Product Selection Guide Corning Cell Culture Media, Sera, Reagents, and Single-use Technology Product Selection Guide Ordering Products Direct from Corning For our U.S. customers who currently have Corning accounts, you can order direct through our Customer Service group or online: t: 800.492.1110, prompt 2 f: 978.442.2476 [email protected] www.corning.com/lifesciences Hours of Operation: Monday to Friday, 8:00 a.m. to 6:00 p.m. (Eastern Standard Time) Customers outside of the U.S., please contact your local Corning distributor, www.corning.com/lifesciences. Phone/Fax Orders For each order, customers should provide the Corning product number, product description, and desired quantity. You should also include your billing and shipping address and your Corning account number. Online Orders In order to purchase Corning products online, please visit the Corning Life Sciences website at www.corning. com/lifesciences. Click on “Order Now, Login” and complete the online registration form. Customers using credit cards may immediately place orders. Full Service Direct accounts with account specific contract pric- ing will need to establish a direct account with Corning Customer Service before online transactions can be made. You can complete the online registration form or contact Corning Customer Service directly at 1.800.492.1110 in order to establish a direct account with Corning. Ordering Products Through our Distribution Partners Customers can purchase Corning products from any one of our more than 50 authorized distributors. See our complete listing of Corning distributors online at www.corning.com/lifesciences. Our distribu- tion partners can offer our customers a variety of value added services from local inventory and service, to managed services, and preferred programs. Please contact your distributor of choice for more details. Pricing Prices shown on the Corning Life Sciences website (in our online www.corning.com/lifesciences catalog) reflect our current suggested U.S. list price. For customer specific pricing information, please contact Corning Customer Service or your authorized Corning Distributor. Product Return Policy To return product, contact your local Customer Service Representative. The order and lot number details are required. Please have this information available to obtain a Return Authorization Number. This Return Authorization Number must be referenced on the outside of the shipping carton. Returns without an approved Return Authorization Number will be refused and returned at the customer’s expense. Media, Sera, Reagents, and Single-use Technology Classical Media .................................................3 Buffered Salt Solutions ........................................ 11 Serum-free/Specialty Media and Solutions ..................... 15 Islet Solutions ................................................ 19 Animal Sera .................................................. 21 Cell Culture Reagents ......................................... 23 Microbiology and Molecular Biology ........................... 31 High Quality Water . 35 Single-use Technology ........................................ 37 Formulations ................................................. 51 Index . 73 1 MEDIA, SERA, REAGENTS, SINGLE-USE TECHNOLOGY Table of Contents Classical Media 3 Cell Culture Reagents 23 DMEM (Dulbecco’s Modification of Eagle’s Medium) 4 Amino Acids and Vitamins 24 DMEM/Ham’s F-12 50/50 Mix 5 Antibiotics and Antimycotics 25 F-12K Nutrient Mixture (Kaighn’s Modification) 5 Buffers 27 Ham's F-10 Medium 5 Enzymatic Cell Dissociation Agents 27 Ham's F-12 Medium 5 Non-enzymatic Cell Dissociation Agents 28 Improved MEM (Richter's Modification) 6 Growth Supplements 28 IMDM (Iscove's Modification of DMEM) 6 Hybridoma Reagents 29 Leibovitz's L-15 (Modification) 6 Insect Reagents 29 MCDB 131 6 Miscellaneous Reagents 30 McCoy's 5A (Iwaketa and Grace Modification) 6 Microbiology and Molecular Biology 31 Medium 199 (Modification) 7 MEM (Minimum Essential Medium) 7 Miller’s LB Broth 32 CMRL 1066 7 S.O.C. Medium 32 MEM (Minimum Essential Medium) Alpha Medium 8 Terrific Broth 32 RPMI 1640 8 Ampicillin Sodium Salt 32 Grace’s Insect Basal Medium (Vaughn Modification) 9 Carbenicillin Disodium Salt 32 Hink’s TNM-FH Medium (Modification) 9 Kanamycin Sulfate 32 Neomycin Sulfate 32 Buffered Salt Solutions 11 Streptomycin Sulfate 32 DPBS (Dulbecco’s Phosphate Buffered Saline) 12 Tetracycline Hydrochloride 33 PBS (Phosphate Buffered Saline) 12 X-Gal 33 HBSS (Hanks’ Balanced Salt Solution) 13 IPTG (Isopropylthiogalactoside) 33 MOPS Buffer 33 Serum-free/Specialty Media and Solutions 15 PBS (Phosphate Buffered Saline) 33 SF Medium 16 SSC Buffer 33 PF Medium 16 SSPE Buffer 33 Corning® transfectagro™ Reduced-serum Medium 16 TAE Buffer 33 LSM (Lymphocyte Separation Medium) 16 TBE Buffer 33 TSB (Tryptic Soy Broth) 16 TE Buffer 34 Hepatocyte Maintenance Medium 17 Tris Buffered Saline 34 Corning hybrigro™ SF Medium 17 Tris-HCI Buffers 34 Corning insectagro® DS2 17 EDTA 34 Corning insectagro Sf9 17 SDS (Sodium Dodecyl Sulfate) 34 Islet Solutions 19 Sodium Acetate 34 Sodium Chloride 34 Islet Solutions and Reagents 20 Cell Separation and Gradient Solutions 20 High Quality Water 35 Porcine Solutions 20 Cell Culture Grade Water 36 Preservation Solutions 20 Molecular Biology Grade Water 36 Animal Sera 21 Reagent Grade Water 36 Fetal Bovine Serum 22 Single-use Technology 37 Bovine Calf Serum, Iron-fortified 22 Collection Containers 39 Donor Horse Serum, U.S. Sourced 22 Cell Expansion Bags 41 Cryopreservation Bags 42 Rocker Cell Culture Bags 43 Tubing Sets 45 Single-use Bags for Corning® HYPERStack® Vessels 46 Tank Liners 47 Film Types 48 Formulations 51 2 Visit www.corning.com/lifesciences/media for additional product and technical information. MEDIA, SERA, REAGENTS, SINGLE-USE TECHNOLOGY Classical Media Growth Promotion and Cytotoxicity Corning® classical media products undergo a variety of tests to determine functionality and toxicity using growth-promotion analysis. Product performance is measured by harvest-to-plant ratios or fold increase according to predetermined specifications. Physiochemical Properties Liquid and powder classical media products are tested to determine pH and osmolality. Classical media powders are also tested for residual moisture. The pH and osmolality tests are performed according to specific procedures in which all equipment is calibrated using standards traceable to the National Institute of Standards and Technology. Biological Tests Quality release testing of Corning liquid classical media products assures low levels of endotoxin, mycoplasma, and an absence of contamination. Endotoxin: Classical media products are tested for bacterial endotoxin using the Limulus Amoebocyte Lysate (LAL) chromogenic assay. For release, each lot of standard liquid classical media must have an endotoxin level of <0.25 EU/mL. Classical media powder products must have an endotoxin level of <0.25 EU/mL when reconstituted to formula weight with low endotoxin water. Sterility: All Corning liquid classical media products are tested for sterility. Mycoplasma: Liquid classical media products are tested for mycoplasma using a large-volume method. For more specific information on claims, visit www.corning.com/lifesciences/media. 3 MEDIA, SERA, REAGENTS, SINGLE-USE TECHNOLOGY DMEM (Dulbecco’s Modification of Eagle’s Medium) Powder Contains 10-013 10-014 10-017 10-027 10-101 10-102 15-013 15-017 15-018 17-204 17-205 17-207 50-003 50-013 90-013 90-113 L-glutamine n n n n n n Sodium pyruvate n n n n n n n n Phenol red n n n n n n n n n n n n n L-cystine/L-methionine n n n n n n n n n n n n n n n Phosphate n n n n n n n n n n n n n n HEPES n n High glucose n n n n n n n n n n n n n Sodium bicarbonate n n n n n n n n n n n n Corning® glutagro™ n n Pyridoxine HCl n n n n Liquid Cat. No. Description Storage Shelf Life Size Qty/Pk 10-013-CV [+] 4.5 g/L Glucose, L-glutamine, sodium pyruvate 2°C to 8°C 18 m 500 mL 6 10-013-CM [+] 4.5 g/L Glucose, L-glutamine, sodium pyruvate 2°C to 8°C 18 m 1L 6 10-013-LX [+] 4.5 g/L Glucose, L-glutamine, sodium pyruvate 2°C to 8°C 18 m 10L 1 10-013-LB [+] 4.5 g/L Glucose, L-glutamine, sodium pyruvate 2°C to 8°C 18 m 20L 1 10-014-CV [+] 1.0 g/L Glucose, sodium pyruvate, L-glutamine 2°C to 8°C 18 m 500 mL 6 10-014-CM [+] 1.0 g/L Glucose, sodium pyruvate, L-glutamine 2°C to 8°C 18 m 1L 6 10-017-CV [+] 4.5 g/L Glucose, L-glutamine; [-] sodium pyruvate 2°C to 8°C 18 m 500 mL 6 10-017-CM [+] 4.5 g/L Glucose, L-glutamine; [-] sodium pyruvate 2°C to 8°C 18 m 1L 6 10-027-CV [+] 25 mM HEPES, 4.5 g/L glucose, L-glutamine; [-] sodium pyruvate 2°C to 8°C 18 m 500 mL 6 10-101-CV [+] Corning glutagro™ supplement, 4.5 g/L glucose, sodium pyruvate, phenol red 2°C to 8°C 12 m 500 mL 6 10-102-CV [+] Corning glutagro supplement, 4.5 g/L glucose, phenol red; [-] sodium pyruvate 2°C to 8°C 12 m 500 mL 6 15-013-CV [+] 4.5 g/L Glucose, sodium pyruvate; [-] L-glutamine 2°C to 8°C 18 m 500 mL 6 15-013-CM [+] 4.5 g/L Glucose, sodium pyruvate; [-] L-glutamine 2°C to 8°C 18 m 1L 6 15-013-LX [+] 4.5 g/L Glucose, sodium pyruvate; [-] L-glutamine 2°C to 8°C 18 m 10L 1 15-013-LB [+] 4.5 g/L Glucose, sodium pyruvate; [-] L-glutamine 2°C to 8°C 18 m 20L 1 15-017-CV [+] 4.5 g/L Glucose; [-] L-glutamine, sodium pyruvate 2°C to 8°C 18 m 500 mL 6 15-017-CM [+] 4.5 g/L Glucose; [-] L-glutamine, sodium pyruvate 2°C to 8°C 18 m 1L 6 15-018-CV [+] 4.5 g/L Glucose, sodium pyruvate, 25 mM HEPES; [-] L-glutamine 2°C to 8°C 18 m 500 mL 6 15-018-CM [+] 4.5 g/L Glucose, sodium pyruvate, 25 mM HEPES; [-] L-glutamine 2°C to 8°C 18 m 1L 6 17-204-CI [+] 4.5 g/L Glucose, sodium pyruvate; [-] L-glutamine, L-methionine, L-cystine 2°C to 8°C 18 m 100 mL 6 17-205-CV [+] 4.5 g/L Glucose, sodium pyruvate; [-] L-glutamine, phenol red 2°C to 8°C 18 m 100 mL 6 17-207-CV [-] Glucose, L-glutamine, sodium pyruvate 2°C to 8°C 18 m 500 mL 6 Powder Cat.
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