The Veracode ADME Core Panel and the Beadxpress® Reader Will Help You Assess the Genetics Behind Drug Absorption, Distribution, Metabolism, and Excretion (ADME)

We’ve got you covered.

The VeraCode® ADME Core Panel. CYP2D6*8 CYP2C9*8 CYP2C9*5 Potential life- saving drug? Or a blind alley?

That’s what you need to find out during pharmacogenetic studies.

Relying on the VeraCode ADME Core Panel and the BeadXpress® Reader will help you assess the genetics behind drug absorption, distribution, metabolism, and excretion (ADME). Investigating associations to drug response, dosing, and safety.

Helping you introduce drugs to market sooner. Bringing personalized medicine closer to reality.

Quickly. Efficiently. Precisely. Quality counts.

Not only does the VeraCode ADME Core Panel provide more than 95% of key ADME Core content, it also delivers the highest quality data. More than 99.5% concordance. More than 99.5% reproducibility.

Plus, Illumina assay chemistry precisely targets critical ADME content, addressing homologous regions. The information you get will be more precise. The decisions you make more informed.

Precisely addressing homologous regions

97% 100% 100% 100% 97% 2C18 100% 2C19 100% 2C9 100% 2C8 2C18 2C19 2C9 2C8

SNP SNP Region-specific primer Region-sEpexcteifnicd epdri mpreorduct Extended product Genotyping Assay Assay oligos Genotyping Assay Assay oligos

CYP2C9*2 (R144C) ) CYP2C9*2 (R144C) ) The CYP450 gene family includes 3 3 a tetrad of closely related genes: CYP2C18, CYP2C19, CYP2C9 and 2 2 CYP2C8. Using an upfront targeting

Norm R approach, a specific gene of interest

Norm R 1 1 can be pulled out from the genome, G/G

G/G G/Tavoiding other homologous regions, 0 G/T T/T 0 23 7 1 and can be analyzed precisely with T/T 23 7 1 Selected 0 0.20 0.40 0.60 0.80 Selected1 excellent discrimination. 0 0.20 0.40 0.60Norm Theta0.80 1 Norm Theta More of what’s important.

Make your metabolism studies more efficient. Get drugs to market faster. Improve human health.

The VeraCode ADME Core Panel offers a better path to metabolism studies. The most complete coverage of critical content. Highest quality data. Simplest, fastest workflow. More core.

ADME Core Genes (34 genes)

ABCB1 CYP2C19 DPYD SLC22A1 TPMT

ABCC2 CYP2C8 GSTM1 SLC22A2 UGT1A1

ABCG2 CYP2C9 GSTP1 SLC22A6 UGT2B15

CYP1A1 CYP2D6 GSTT1 SLCO1B1 UGT2B17

CYP1A2 CYP2E1 NAT1 SLCO1B3 UGT2B7

CYP2A6 CYP3A4 NAT2 SLCO2B1 VKORC1

CYP2B6 CYP3A5 SLC15A2 SULT1A1

The essential genes that populate the VeraCode ADME Core Panel include several with well-known associations to model drugs. For example, CYP2D6 has been associated with the metabolism of certain antidepressants, antipsychotics, cancer drugs like Tamoxifen and many others, CYP2C19 has been associated with the metabolism of the anti-platelet Plavix, and CYP2C9 and VKORC1 are most well known for their association to warfarin sensitivity.

For a detailed VeraCode ADME Core SNP list, please contact your account manager or visit www.illumina.com/veracodeADME. Speed matters.

One day. Why wait longer when you can analyze key ADME content in under 8 hours? The simple workflow, with built- in sample tracking and internal controls, accelerates your studies. The easy-to-use software interface manages a run from setup through data analysis and translation. It even provides useful reports.

Workflow

A B C A B C

AnnealingAnnealing AnnealingMix Mix Mix Universal PCR TargetingTargeting MixTargeting Mix Mix OligoOligo Oligo

Single Plate Transfer

Extension and Automatic Analysis Targeting Reaction Hybridization Scan Ligation Reaction and Translation

Pre-PCR (2 hr 15 min) Post-PCR (5 hr 15 min)

Simple workflow.

An initial extension with biotinylated primers specifically targets regions of interest. Products are captured onto paramagnetic particles for allele-specific primer extension and ligation followed by universal PCR. A single plate transfer hybridizes amplified material to VeraCode Beads for scanning on the BeadXpress Reader. Thirty-four genes are assayed within 8 hours with less than 2.5 hours of technical hands-on time. Quickly. Efficiently. Precisely.

Let the coverage begin. Learn more at www.illumina.com/veracodeADME Learn more at www.illumina.com/veracodeADME

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FOR RESEARCH USE ONLY

© 2010 Illumina, Inc. All rights reserved. Illumina, illuminaDx, Solexa, Making Sense Out of Life, Oligator, Sentrix, GoldenGate, GoldenGate Indexing, DASL, BeadArray, Array of Arrays, Infinium, BeadXpress, VeraCode, IntelliHyb, iSelect, CSPro, GenomeStudio, Genetic Energy, HiSeq, and HiScan are registered trademarks or trademarks of Illumina, Inc. All other brands and names contained herein are the property of their respective owners. Pub No. 182-2010-001 Current as of 19 March 2010