OVGP1 Antibody Cat. No.: 4763
OVGP1 Antibody
Immunofluorescence of OVGP1 in 293 cells with OVGP1 Immunocytochemistry of OVGP1 in 293 cells with OVGP1L antibody at 2.5 μg/mL. antibody at 2.5 μg/mL.
Immunofluorescence of OVGP1 in Hek293 cells with OVGP1 antibody at 20 μg/mL.
Green: OVGP1 Antibody (4763) Blue: DAPI staining
Specifications
HOST SPECIES: Rabbit
September 27, 2021 1 https://www.prosci-inc.com/ovgp1-antibody-4763.html SPECIES REACTIVITY: Human, Mouse, Rat
Predicted species reactivity based on immunogen sequence: Sheep: (94%), Bovine: (94%), HOMOLOGY: Pig: (79%)
OVGP1 antibody was raised against an 18 amino acid synthetic peptide from near the amino terminus of human OVGP1. IMMUNOGEN:
The immunogen is located within amino acids 60 - 110 of OVGP1.
TESTED APPLICATIONS: ELISA, ICC, IF, WB
OVGP1 antibody can be used for the detection of OVGP1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL. APPLICATIONS: Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
POSITIVE CONTROL: 1) Cat. No. 1309 - Human Placenta Tissue Lysate
2) Cat. No. 17-010 - HEK293 Cell Slide
Predicted: 75 kDa PREDICTED MOLECULAR WEIGHT: Observed: Due to extensive post-translational modification, OVGP1 migrates at ~140 kDa in SDS-PAGE.
Properties
PURIFICATION: OVGP1 Antibody is affinity chromatography purified via peptide column.
CLONALITY: Polyclonal
ISOTYPE: IgG
CONJUGATE: Unconjugated
PHYSICAL STATE: Liquid
BUFFER: OVGP1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION: 1 mg/mL
OVGP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one STORAGE CONDITIONS: year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
OFFICIAL SYMBOL: OVGP1
OVGP1 Antibody: EGP, OGP, MUC9, CHIT5, Oviduct-specific glycoprotein, Estrogen- ALTERNATE NAMES: dependent oviduct protein
ACCESSION NO.: NP_002548
September 27, 2021 2 https://www.prosci-inc.com/ovgp1-antibody-4763.html PROTEIN GI NO.: 58386720
GENE ID: 5016
USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background and References
OVGP1 Antibody: Oviductins belong to a family of glycoproteins that have been suggested to play several roles during the early processes of reproduction. OVGP1 is a large carbohydrate-rich, epithelial glycoprotein with numerous O-glycosylation sites within BACKGROUND: threonine, serine, and proline-rich tandem repeats. The protein is secreted from non- ciliated oviductal epithelial cells and associates with ovulated oocytes, blastomeres, and spermatozoan acrosomal regions. At least two isoforms of OVGP are known to exist.
1) Buhi WC. Characterization and biological roles of oviduct-specific, oestrogen-dependent REFERENCES: glycoprotein. Reproduction123:355-62.
2) Arias EB, Verhage HG and Jaffe RC. Complementary deoxyribonucleic acid cloning and molecular characterization of an estrogen-dependent human oviductal glycoprotein. Biol. Reprod.1994; 51:685-94.
ANTIBODIES FOR RESEARCH USE ONLY.
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September 27, 2021 3 https://www.prosci-inc.com/ovgp1-antibody-4763.html