Regional Status Pest Surveillance in the Context of ISPM No. 6

Total Page:16

File Type:pdf, Size:1020Kb

Regional Status Pest Surveillance in the Context of ISPM No. 6 RAP PUBLICATION 2012/09 Regional status of pest surveillance in the context of ISPM No. 6: International Standard for Phytosanitary Measures – Guidelines for Pest Surveillance Analysis of the responses to the Implementation Review and Support System (IRSS) questionnaire from APPPC countries viii RAP PUBLICATION 2012/09 Regional status of pest surveillance in the context of ISPM No. 6: International Standard for Phytosanitary Measures – Guidelines for Pest Surveillance Analysis of the responses to the Implementation Review and Support System (IRSS) questionnaire from APPPC countries FOOD AND AGRICULTURE ORGANIZATION OF THE UNITED NATIONS REGIONAL OFFICE FOR ASIA AND THE PACIFIC Bangkok, 2012 i The designation employed and the presentation of material in this information product do not imply the expression of any opinion whatsoever on the part of the Food and Agriculture Organization of the United Nations (FAO) concerning the legal or development status of any country, territory, city or area or of its authorities, or concerning the delimitation of its frontiers or boundaries. the mention of specific companies or products of manufacturers, whether or not these have been patented, does not imply that these have been endorsed or recommended by FAO in preference to others of a similar nature that are not mentioned. ISBN 978-92-5-107229-5 All rights reserved. Production and dissemination of material in this information product for educational or other non-commercial purposes are authorized without any prior written permission from the copyright holders provided the source is fully acknowledged. Reproduction of material in this information product for resale or other commercial purposes is prohibited without written permission of the copyright holders. Applications for such permission should be addressed to: Chief Electronic Publishing Policy and Support Branch Communication Division FAO Viale delle Terme di Caracalla, 00153 Rome, Italy or by e-mail to: [email protected] © FAO 2012 For a copy of this publication, please write to: Piao Yongfan FAO Regional Office for Asia and the Pacific Maliwan Mansion, 39 Phra Atit Road Bangkok 10200 THAILAND Tel: (+66) 2 697 4268 Fax: (+66) 2 697 4445 E-mail: [email protected] ii Foreword A fundamental activity of national plant protection organizations is to conduct pest surveillance. For the purpose of international trade, the results of pest surveillance provide the basis for pest listing, identification of pest status, pest categorization and the conduct of pest risk analyses. In 1997, the International Plant Protection Convention issued the International Standard for Phytosanitary Measures (ISPM) No. 6: Guidelines for surveillance to assist national organizations in the implementation of this critical activity. In 2011, it was decided to review these guidelines and to identify challenges faced by the contracting parties for implementation, as well as identify resources for resolving those challenges. At the request of the Standards Committee of the IPPC, the Implementation Review and Support System (IRSS) will catalogue the challenges faced and make recommendations to the review panel of ISPM No. 6 on ways to improve the standard. It will also gather examples of best practice globally so that appropriate training material and manuals might be prepared under the capacity development programme of the IPPC. For the purpose of this review, a questionnaire was developed and distributed among national plant protection organizations. I am pleased to report that a total of 17 countries from the Asia and the Pacific region responded to this call and completed the questionnaire, as well as listing their resources and best practices. The results from this survey were compiled and analyzed, and are presented in this publication, which reflects the current regional status of the surveillance. Best practices of the surveillance and main constraints, challenges as well as issues that exist in implementation of the surveillance were also discussed and updated at a workshop held in Chiang Rai, Thailand from 31 January to 3 February 2012. It is hoped that the experiences with ISPM No. 6 in the Asia and the Pacific region will not only facilitate the work of the ISPM No. 6 review panel, but that it will also foster greater collaboration and exchanges among the countries of the region. Hiroyuki Konuma Assistant Director-General and FAO Regional Representative for Asia and the Pacific Bangkok, February 2012 iii iv Table of contents Page Foreword by the Assistant Director-General and FAO Regional Representative for Asia and the Pacific .............................................................................................................................. iii List of acronyms............................................................................................................................. vii 1. Introduction ......................................................................................................................... 1 2. Questionnaire ....................................................................................................................... 3 A. Policy and legislative environment ............................................................................... 3 B. Organizational structure, competences and culture ....................................................... 7 C. Documented procedures ................................................................................................ 11 D. General surveillance ...................................................................................................... 13 E. Specific surveys............................................................................................................. 16 Pest diagnostics ............................................................................................................. 20 F. Resources ...................................................................................................................... 23 G. Open ended feedback .................................................................................................... 28 3. Technical resources.............................................................................................................. 30 Tools ...................................................................................................................................... 30 Training materials .................................................................................................................. 31 Presentations .......................................................................................................................... 32 Videos .................................................................................................................................... 32 E-learning .............................................................................................................................. 32 Manuals ................................................................................................................................. 32 Guidelines .............................................................................................................................. 32 Standard operating procedures .............................................................................................. 33 Advocacy materials ............................................................................................................... 33 Other ...................................................................................................................................... 33 Names and addresses of respondents .................................................................................... 45 4. Best practices ....................................................................................................................... 46 Surveys in relation to plant quarantine and international trade ............................................. 46 Surveys in relation to pest detection and management ......................................................... 46 5. Conclusions and recommendations.................................................................................... 92 Annex 1: Country responses to open questions .......................................................................... 96 Annex 2: Summary slides presented at the workshop ............................................................... 108 v vi List of acronyms ACIAR Australian Centre for International Agricultural Research ALPP Areas of low pest prevalence APPPC Asia Pacific Plant Protection Convention ASEAN Association of Southeast Asian Nations AWM Area wide management CABI Centre for Agriculture and Biosciences International CPPQ Crop Protection and Plant Quarantine Division (Malaysia) DAFF Department of Agriculture, Fisheries and Forestry DNA Designated National Authority DOA Department of Agriculture EPPO European and Mediterranean Plant Protection Organization ETL Economic threshold level EU European Union FAO Food and Agriculture Organization of the United Nations FFS Farmer Field School GAP Good agricultural practices GIS Geographic information system HRSS High risk site surveillance IPM Integrated pest management IPPC International Plant Protection Convention IRSS Implementation Review and Support System ISPM International Standard for Phytosanitary Measures MOA Ministry of Agriculture NATESC National Agro-Tech Extension Service
Recommended publications
  • Mixta Gen. Nov., a New Genus in the Erwiniaceae
    RESEARCH ARTICLE Palmer et al., Int J Syst Evol Microbiol 2018;68:1396–1407 DOI 10.1099/ijsem.0.002540 Mixta gen. nov., a new genus in the Erwiniaceae Marike Palmer,1,2 Emma T. Steenkamp,1,2 Martin P. A. Coetzee,2,3 Juanita R. Avontuur,1,2 Wai-Yin Chan,1,2,4 Elritha van Zyl,1,2 Jochen Blom5 and Stephanus N. Venter1,2,* Abstract The Erwiniaceae contain many species of agricultural and clinical importance. Although relationships among most of the genera in this family are relatively well resolved, the phylogenetic placement of several taxa remains ambiguous. In this study, we aimed to address these uncertainties by using a combination of phylogenetic and genomic approaches. Our multilocus sequence analysis and genome-based maximum-likelihood phylogenies revealed that the arsenate-reducing strain IMH and plant-associated strain ATCC 700886, both previously presumptively identified as members of Pantoea, represent novel species of Erwinia. Our data also showed that the taxonomy of Erwinia teleogrylli requires revision as it is clearly excluded from Erwinia and the other genera of the family. Most strikingly, however, five species of Pantoea formed a distinct clade within the Erwiniaceae, where it had a sister group relationship with the Pantoea + Tatumella clade. By making use of gene content comparisons, this new clade is further predicted to encode a range of characters that it shares with or distinguishes it from related genera. We thus propose recognition of this clade as a distinct genus and suggest the name Mixta in reference to the diverse habitats from which its species were obtained, including plants, humans and food products.
    [Show full text]
  • Carica Papaya L.) in Peninsular Malaysia
    Journal of Fundamental and Applied Sciences Research Article Special Issue ISSN 1112-9867 Available online at http://www.jfas.info FIRST REPORT OF CHRYSEOBACTERIUM INDOLOGENES AS CAUSAL AGENT FOR CROWN ROT OF PAPAYA (CARICA PAPAYA L.) IN PENINSULAR MALAYSIA B. N. M. Din1, J. Kadir1, M. S. Hailmi2,*, K. Sijam1, N. A. Badaluddin2 and Z. Suhaili2 1Plant Protection Department, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia 2School of Agriculture Science and Biotechnology, Faculty of Bioresources and Food Science, Universiti Sultan Zainal Abidin, Tembila Campus, 22200 Besut, Terengganu, Malaysia Published online: 08 August 2017 ABSTRACT Bacterial strains were isolated from papaya plants showing the crown rot symptoms in peninsular Malaysia. Greasy and water-soaked lesions were observed on petiole axis, young stems and buds of the plants. Bacteria were then identified using the Biolog system showed that the bacterium was Chryseobacterium indolegenes with a similarity (SIM) index of between 0.5 and 0.74 at 24 h of incubation followed by standard morphological and biochemical tests. The isolates were then confirmed by Polymerase Chain Reaction (PCR) and sequencing of the 16S rRNA gene and was successfully identified as C. indologenes with a 100% sequence similarity with reference strain (C. indolegenes strain LMG 8337; GenBank Acc. No: NR_042507.1). C indolegenes was consistently isolated from diseased papaya plants and the pathogenicity was confirmed by Koch’s postulate. Keywords: papaya crown rot; Chryseobacterium indologenes. ___________________________________________________________________________ Author Correspondence, e-mail: [email protected] doi: http://dx.doi.org/10.4314/jfas.v9i2s.51 1. INTRODUCTION Papaya (Carica papaya L.) is widely known as an aggressive plant and has the potential to spread quickly, semi-woody tropical herbs [1] and one of the major global fruit crops that is Journal of Fundamental and Applied Sciences is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
    [Show full text]
  • FINAL REPORT Groundwater Chemistry and Microbial Ecology Effects on Explosives Biodegradation
    FINAL REPORT Groundwater Chemistry and Microbial Ecology Effects on Explosives Biodegradation SERDP Project ER-1378 SEPTEMBER 2008 Dr. Mark E. Fuller Dr. Robert J. Steffan Shaw Environmental, Inc. This report was prepared under contract to the Department of Defense Strategic Environmental Research and Development Program (SERDP). The publication of this report does not indicate endorsement by the Department of Defense, nor should the contents be construed as reflecting the official policy or position of the Department of Defense. Reference herein to any specific commercial product, process, or service by trade name, trademark, manufacturer, or otherwise, does not necessarily constitute or imply its endorsement, recommendation, or favoring by the Department of Defense. Final Report Table of Contents List of Abbreviations ····················································································································ii List of Tables ·······························································································································iv List of Figures·····························································································································vii Acknowledgements·······················································································································x I. EXECUTIVE SUMMARY ·······································································································1 II. PROJECT OBJECTIVES·········································································································3
    [Show full text]
  • Aremu BR.Pdf (5.310Mb)
    PHYLOGENETIC ANALYSES OF SPECIES-SPECIFIC MACERGENS IN SOUTH AFRICAN EXPORTABLE VEGETABLES L NWu ~ L!J.f!B_ARV BY BUKOLA RHODA AREMU A Thesis Submitted in Fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY (BIOLOGY) LIBRARY r,JIJ\Fl!H:1 JG CAMPUS CALL NO.: 2019 -07- 1 5 ACC. "1O .: : i'.&ORTH!-W IEST UNIVERSITY DEPARTMENT OF BIOLOGICAL SCIENCES FACULTY OF SCIENCE, AGRICULTURE AND TECHNOLOGY, NORTH-WEST UNIVERSITY, MAFIKENG CAMPUS, SOUTH AFRICA Supervisor: Professor Olubukola 0. Babalola 2015 NORTH-WEST UNIVERSITY • I YUNIBESITI YA BOKONE -BOPHIRIMA NOORDWES-UNIVERS ITEIT It all starts here '" DECLARATION I, the undersigned, declare that this thesis submitted to the North-West University for the degree of Doctor of Philosophy in Biology in the Faculty of Science, Agriculture and Technology, School of Environmental and Health Sciences, and the work contained herein is my original work with exception of the citations and that this work has not been submitted at any other University in part or entirety for the award of any degree. Student AREMU, Bukola Rhoda S1gnature. ::..:::~ .AJA...... ... .. Date ..<:2--q/oL?(. .. ... .. .... ... .lb.. ... .... .... .... Supervisor BABALOLA, 0.0. (Professor) Date ... '+1/ O.tf I(&. ....... II NWU· ~ · lueRARY_ DEDICATION Thi s thesis is dedicated to six indispensabl e people of my li fe ; my lovely husband O luwole Samuel Aremu, three j ewels Favour, Mercy and Grace, my dearest daddy Amos A lade Amoa and late mummy Dorcas Mosunmola Amoa. Ill ACKNOWLEDGEMENTS I would like to express my gratitude to those who assisted me. First, my supervisor Prof. Olubukola Oluranti Babalola for her support, care, directions and timely advice to ensure that this project is brought to completion.
    [Show full text]
  • Download This PDF File
    Covenant Journal of Physical & Life Sciences (CJPL) Vol. 5 No. 1, June 2017 An Open Access Journal available online Covenant Journal of Physical & Life Sciences Vol. 5 No. 1, June. 2017 A Publication of Covenant University Editor-in-Chief: Prof. Louis Egwari [email protected] Managing Editor: Edwin O. Agbaike [email protected] Htt//journals.covenantuniversity.edu.ng/cjpl/ © 2017, Covenant University Journals All rights reserved. No part of this publication may be reproduced, stored in a retrieval system or transmitted in any form or by any meams, electronic, electrostatic, magnetic tape, mechanical, photocopying, recording or otherwise, without the prior written permission of the publisher. It is a condition of publication in this journal that manuscripts have not been published or submitted for publication and will not be submitted or published elsewhere. Upon the acceptance of articles to be published in this journal,the author(s) are required to transfer copyright of the article to the publisher. ISSN: Print 2354 – 3574 Electronics 2354 – 3485 Published by Covenant University Journals, Covenant University, Canaanland, Km 10, Idiroko Road, P.M.B. 1023, Ota, Ogun State, Nigeria Printed by Covenant University Press ii Articles Erwinia Rots and Presence of Pathogenic Bacteria in Symptomless Fruits and Vegetables Babalola, F. A. & Egwari, L. O. 1 Comparative Haematological and Serum Biochemical Evaluation of Rats Fed with Hydro-ethanolic Leaves Extracts of Moringa oleifera and Telfairia occidentalis Udo E.F, Olantunji O.I, Abayomi F.T. & IdowuAM,Abiodun A.O 17 Prevalence and Risk Factors Associated with Human Cytomegalovirus and Human Immunodeficiency Virus Coinfection in Pregnant Women in Ilorin, Nigeria Olatunji, M.
    [Show full text]
  • Evaluation of FISH for Blood Cultures Under Diagnostic Real-Life Conditions
    Original Research Paper Evaluation of FISH for Blood Cultures under Diagnostic Real-Life Conditions Annalena Reitz1, Sven Poppert2,3, Melanie Rieker4 and Hagen Frickmann5,6* 1University Hospital of the Goethe University, Frankfurt/Main, Germany 2Swiss Tropical and Public Health Institute, Basel, Switzerland 3Faculty of Medicine, University Basel, Basel, Switzerland 4MVZ Humangenetik Ulm, Ulm, Germany 5Department of Microbiology and Hospital Hygiene, Bundeswehr Hospital Hamburg, Hamburg, Germany 6Institute for Medical Microbiology, Virology and Hygiene, University Hospital Rostock, Rostock, Germany Received: 04 September 2018; accepted: 18 September 2018 Background: The study assessed a spectrum of previously published in-house fluorescence in-situ hybridization (FISH) probes in a combined approach regarding their diagnostic performance with incubated blood culture materials. Methods: Within a two-year interval, positive blood culture materials were assessed with Gram and FISH staining. Previously described and new FISH probes were combined to panels for Gram-positive cocci in grape-like clusters and in chains, as well as for Gram-negative rod-shaped bacteria. Covered pathogens comprised Staphylococcus spp., such as S. aureus, Micrococcus spp., Enterococcus spp., including E. faecium, E. faecalis, and E. gallinarum, Streptococcus spp., like S. pyogenes, S. agalactiae, and S. pneumoniae, Enterobacteriaceae, such as Escherichia coli, Klebsiella pneumoniae and Salmonella spp., Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Bacteroides spp. Results: A total of 955 blood culture materials were assessed with FISH. In 21 (2.2%) instances, FISH reaction led to non-interpretable results. With few exemptions, the tested FISH probes showed acceptable test characteristics even in the routine setting, with a sensitivity ranging from 28.6% (Bacteroides spp.) to 100% (6 probes) and a spec- ificity of >95% in all instances.
    [Show full text]
  • Carica Papaya)
    Inuence of native endophytic bacteria on the growth and bacterial crown rot tolerance of papaya (Carica papaya) Mark Paul Selda Rivarez ( [email protected] ) National Institute of Biology: Nacionalni institut za biologijo https://orcid.org/0000-0002-8544-5740 Elizabeth P. Parac Caraga State University Shajara Fatima M. Dimasingkil Mindanao State University Eka Mirnia Indonesian Agency for Agricultural Research and Development Pablito M. Magdalita ( [email protected] ) University of the Philippines Los Banos Research Article Keywords: papaya bacterial crown rot, Erwinia, Kosakonia, Sphingomonas, Bacillus, endophytic bacteria, autoclaved culture metabolites Posted Date: May 27th, 2021 DOI: https://doi.org/10.21203/rs.3.rs-559032/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Version of Record: A version of this preprint was published at European Journal of Plant Pathology on August 14th, 2021. See the published version at https://doi.org/10.1007/s10658-021-02345-1. Page 1/23 Abstract The native plant microbiome is composed of diverse communities that inuence its overall health, with some species known to promote plant growth and pathogen resistance. Here, we show the antibacterial and growth promoting activities of autoclaved culture metabolites (ACMs) from native endophytic bacteria (NEB) in a papaya cultivar that is tolerant to bacterial crown rot (BCR) caused by Erwinia mallotivora . Initially, bacterial colonization in recovering tissues of this cultivar was observed before onset of tissue regeneration or 'regrowth'. We further isolated and characterized these bacteria and were able to identify two culturable stem NEB under genera Kosakonia (EBW), related to Enterobacter , and Sphingomonas (EBY).
    [Show full text]
  • X2018;Nissabacter Archeti’
    AIX-MARSEILLE UNIVERSITE FACULTE DE MEDECINE-LA TIMONE ECOLE DOCTORALE DES SCIENCES DE LA VIE ET DE LA SANTE Présentée et soutenue le 24 Novembre 2017 Par En vue de l’obtention du grade de Docteur de l’Université Aix-Marseille Spécialité : Génomique et Bio-informatique REAL-TIME GENOMICS TO DECIPHER ATYPICAL BACTERIA IN CLINICAL MICROBIOLOGY COMPOSITION DU JURY Président du Jury Professeur Anthony Levasseur Examinateur Professeur Ruimy Raymond Rapporteur1 Professeur Marie Kempf Rapporteur2 Professeur Estelle Jumas-Bilak Directeur de Thèse Professeur Jean-Marc Rolain Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes URMITE CNSR-IRD UMR7278, IHU MEDITERRANEE INFECTION AIX-MARSEILLE UNIVERSITE FACULTE DE MEDECINE-LA TIMONE ECOLE DOCTORALE DES SCIENCES DE LA VIE ET DE LA SANTE Présentée et soutenue le 24 Novembre 2017 Par En vue de l’obtention du grade de Docteur de l’Université Aix-Marseille Spécialité : Génomique et Bio-informatique REAL-TIME GENOMICS TO DECIPHER ATYPICAL BACTERIA IN CLINICAL MICROBIOLOGY COMPOSITION DU JURY Président du Jury Professeur Anthony Levasseur Examinateur Professeur Ruimy Raymond Rapporteur1 Professeur Marie Kempf Rapporteur2 Professeur Estelle Jumas-Bilak Directeur de Thèse Professeur Jean-Marc Rolain Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes URMITE CNSR-IRD UMR7278, IHU MEDITERRANEE INFECTION 1 CONTENT Avant-propos Résumé /Abstract Introduction Chapter I: Review Articles I: Real-time genomics and the impact of bacterial genome recombination in clinical microbiology Kodjovi D. Mlaga, Seydina M. Diene, R. Ruimy, J-M Rolain. (Submitted in Genome Biology and Evolution) Chapter II: Comparative genomic applied in clinical microbiology Articles II: Using MALDI-TOF MS typing method to decipher outbreak: the case of Staphylococcus saprophyticus causing urinary tract infections (UTIs) in Marseille, France.
    [Show full text]
  • USAID Cooperative Agreement No: EPP‐A‐00‐0400016‐00
    USAID Cooperative Agreement No: EPP‐A‐00‐0400016‐00 Management Entity for IPM CRSP: Office of International Research, Education, & Development (OIRED) Virginia Tech, 526 Prices Fork Road, Blacksburg, VA 24060, www.oired.vt.edu/ipmcrsp/ Integrated Pest Management Collaborative Research Support Program FY 2009 Annual Report October 1, 2008 – September 30, 2009 Report Coordinators R. Muniappan Larry Vaughan Miriam Rich Annie Steed USAID Cooperative Agreement No: EPP-A-00-0400016-00 IPM CRSP Management Entity S.K. De Datta, Administrative PI, Director of OIRED, Associate Vice President for International Affairs, Virginia Tech R. Muniappan, Program Director Larry Vaughan, Associate Program Director Maria Elisa Christie, Women in Development Program Director Annie Steed, Research Associate Miriam Rich, Communications Coordinator Debbie Francis, Program Coordination Assistant IPM CRSP Program Advisory Board (PAB) Joseph Culin, Clemson University John Dooley, Virginia Tech Alma Hobbs, Virginia State University Bruce McPheron, Pennsylvania State University Bobby Moser, The Ohio State University Larry Olsen, Michigan State University (Chair) Robert Hedlund, USAID S. K. De Datta, Virginia Tech R. Muniappan, Virginia Tech Larry Vaughan, Virginia Tech IPM CRSP Technical Committee Jeffrey Alwang, Virginia Tech Kitty Cardwell, USDA, NIFA Mark Erbaugh, The Ohio State University Michael Hammig, Clemson University Samuel Kyamanywa, Makerere University, Uganda Karim Maredia, Michigan State University Wondi Mersie, Virginia State University Sally Miller, The Ohio State University Donald Mullins, Virginia Tech George Norton, Virginia Tech (Chair) Douglas Pfeiffer, Virginia Tech Edwin Rajotte, Pennsylvania State University Naidu Rayapati, Washington State University Stanley Woods, IFPRI Sue Tolin, Virginia Tech Yulu Xia, North Carolina State University Robert Hedlund, USAID S.K. De Datta, Virginia Tech R.
    [Show full text]
  • Erwinia Species Identification Using Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry
    Journal of Plant Pathology (2017), 99 (Special issue), 121-129 Edizioni ETS Pisa, 2017 121 ERWINIA SPECIES IDENTIFICATION USING MATRIX-ASSISTED LASER DESORPTION IONIZATION-TIME OF FLIGHT MASS SPECTROMETRY F. Rezzonico1,2, B. Duffy1,2, T.H.M. Smits1,2 and J.F. Pothier1,2 1 Environmental Genomics and System Biology Research Group, Institute of Natural Resource Sciences, Zürich University of Applied Sciences (ZHAW), Campus Grüental, 8820 Wädenswil, Switzerland 2 Agroscope Changins-Wädenswil ACW, Plant Protection Division, Schloss, 8820 Wädenswil, Switzerland SUMMARY INTRODUCTION Rapid and reliable identification of plant pathogenic Erwinia (Winslow et al., 1920) is a genus of the Entero- bacteria is critical for effective implementation of phytos- bacteriaceae family that was originally created to unite anitary measures. The genus Erwinia includes a number of all Gram-negative, nonsporulating, fermentative, peritri- economically important plant pathogens such as fire blight chously flagellated plant-pathogenic bacteria (Kwon et al., agent Erwinia amylovora or Asian pear pathogen Erwinia 1997). Since its inception, the genus has undergone several pyrifoliae, together with closely related plant epiphytes of taxonomical rearrangements following refined phenotypic unknown pathogenicity or even with a potential use for characterization or DNA sequence analysis, with many biological control like Erwinia tasmaniensis or Erwinia bill- species being transferred to other genera such as Pecto- ingiae, respectively. Current laboratory methods to achieve
    [Show full text]
  • Erwinia Oleae Sp. Nov., Isolated from Olive Knots Caused by Pseudomonas Savastanoi Pv
    IJSEM Papers in Press. Published December 24, 2010 as doi:10.1099/ijs.0.026336-0 1 Erwinia oleae sp. nov., isolated from olive knots caused by Pseudomonas savastanoi pv. 2 savastanoi 3 4 Chiaraluce Moretti*1, Taha Hosni*1, Katrien Vandemeulebroecke2, Carrie Brady3, Paul De Vos2, 5 Roberto Buonaurio1 and Ilse Cleenwerck2 6 7 * C. Moretti and T. Hosni contributed equally to this work 8 1 Dipartimento di Scienze Agrarie e Ambientali, University of Perugia, Perugia, Italy 9 2 BCCM/LMG Bacteria Collection, Ghent University, Ghent, Belgium 10 3 LM-UGent, Laboratory of Microbiology, Faculty of Sciences, Ghent University, Ghent, Belgium 11 12 13 Running title: Erwinia oleae sp. nov. from olive knots 14 15 16 17 Correspondence: Chiaraluce Moretti, [email protected] 18 19 20 21 The GenBank accession number for the 16S rRNA gene sequence of DAPP-PG 531T (= LMG 22 25322T = DSM 23398T) is GU810925. The accession numbers for the atpD, gyrB, infB and rpoB 23 gene sequences of DAPP-PG 531T, DAPP-PG 672 (= LMG 25321) and CECT 5264 (= LMG 24 25328) are GU991653-GU991656, HM439616-HM439619, HM439612-HM439615 respectively. 25 26 27 Summary 28 Three endophytic bacterial isolates were obtained in Italy from olive knots caused by Pseudomonas 29 savastanoi pv. savastanoi. Phenotypic tests in combination with 16S rRNA gene sequence analysis 30 indicated a phylogenetic position of these isolates in the genus Erwinia or Pantoea, and revealed 31 two other strains with highly similar 16S rRNA gene sequences (> 99 %), CECT 5262 and CECT 32 5264, obtained in Spain from olive knots. Rep-PCR DNA fingerprinting of the five strains from 33 olive knots with BOX, ERIC and REP primers revealed three groups of profiles that were highly 34 similar to each other.
    [Show full text]
  • Exploring the Genetic Basis for Host Specific Virulence and Pathogenicity in Erwinia Tracheiphila
    Iowa State University Capstones, Theses and Graduate Theses and Dissertations Dissertations 2020 Exploring the genetic basis for host specific virulence and pathogenicity in Erwinia tracheiphila Olakunle Olawole Iowa State University Follow this and additional works at: https://lib.dr.iastate.edu/etd Recommended Citation Olawole, Olakunle, "Exploring the genetic basis for host specific virulence and pathogenicity in Erwinia tracheiphila" (2020). Graduate Theses and Dissertations. 18577. https://lib.dr.iastate.edu/etd/18577 This Dissertation is brought to you for free and open access by the Iowa State University Capstones, Theses and Dissertations at Iowa State University Digital Repository. It has been accepted for inclusion in Graduate Theses and Dissertations by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. Exploring the genetic basis for host specific virulence and pathogenicity in Erwinia tracheiphila by Olakunle I. Olawole A dissertation submitted to the graduate faculty in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY Major: Plant Pathology Program of Study Committee: Gwyn A. Beattie, Major Professor Mark L. Gleason Steven A. Whitham Alison E. Robertson Kyaw J. Aung The student author, whose presentation of the scholarship herein was approved by the program of study committee, is solely responsible for the content of this thesis. The Graduate College will ensure this thesis is globally accessible and will not permit alterations after a degree is conferred. Iowa State University Ames, Iowa 2020 Copyright © Olakunle I. Olawole, 2020. All rights reserved. ii DEDICATION To God Almighty – my strength, shield and exceeding great reward; without You by my side, the journey wouldn’t have been this glorious.
    [Show full text]