Avian Influenza A/H5 Detection Kit for Taqman® Probe Assays

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Kit Part No. ASAY-ASY-0112 Avian Influenza A/H5 ® Detection Kit for TaqMan Probe Assays This kit and accompanying information is designed for the detection of avian influenza A/H5. This kit is for research use only and not for use in human diagnostic or epi- demiologic procedures. Refer to the Freeze-Dried Reagent Detection Kit Instruction Booklet for TaqMan Probes (ASAY-PRT-0130, Reverse Transcription PCR protocol for RNA) for protocols, reagent setup, results analysis, and additional information. Sample Specificity Isolated RNA This kit has demonstrated specific detection of avian influenza A/H5 versus a panel of 41 organisms, both related and unrelated (see back). Specificity was 100%. Software The instructions shown below are given for three R.A.P.I.D.® instrument models and software. Use the appropriate instructions for your system. System number can be found on the serial plate. Model 7200 Models 9200 and LT Select Influenza A Target 1 from the Select Influenza A Target 1 from the Batch Test menu on the software software wizard screen. If outcome of screen. If outcome of test is positive, test is positive, then run the Avian H5 then run the Avian H5 Target 1 test. Target 1 test. ASAY-PRT-0168-02 Avian Influenza A/H5 Detection Kit Validated Strains This validated Influenza A Target 1 assay shows no cross-reactivity against the following organisms and strains: Adenovirus, n=2 Escherichia coli Human metapneumovirus, n=2 Shigella dysenteriae Human rhinovirus, n=2 Salmonella enterica serovar Typhi Influenza B, n=2 Parainfluenza virus type 1, n=2 Parainfluenza virus type 2, n=2 Parainfluenza virus type 3, n=2 Respiratory syncytial virus, n=2 This validated Avian Influenza (H5) Target 1 assay shows no cross-reactivity against the following organisms and strains: H3N2 (human), n=7 Parainfluenza virus type 1 Bacillus anthracis H1N1 (human), n=6 Parainfluenza virus type 2 Burkholderia mallei H1N2 (human), n=1 Parainfluenza virus type 3 Vaccinia (orthopox) H6N? (avian), n=1 Influenza B Yersinia pestis H7N? (avian), n=1 Adenovirus Francisella tularensis H10N? (avian), n=5 Human metapneumovirus Coxiella burnetii N11N? (avian), n=1 Human rhinovirus Brucella melitensis Respiratory syncytial virus Rickettsia prowazekii Human DNA E.coli O157:H7 (EHEC) Salmonella enterica serovar Typhi Shigella dysenteriae This validated Avian Influenza (H5) Target 1 assay was tested against and recog- nizes the following strains: H5N1 (human), n=12 H5N1 (avian), n=12 H5N1 (feline), n=2 Notes: The Influenza A Target 1 assay was positive when tested against 10 influenza A isolates from humans. Specificity testing of Influenza A Target 1 with non-H5 hemagglutinin subtypes is cur- rently in progress. Contact ITI for further information. n = number of independent isolates tested Avian Influenza A/H5 Detection Kit.

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Human Metapneumovirus Pcr
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