Common Outbreak Organisms
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Human Metapneumovirus Pcr
Lab Dept: Microbiolgy/Virology Test Name: HUMAN METAPNEUMOVIRUS PCR General Information Lab Order Codes: HMPV Synonyms: hMPV PCR; Metapneumovirus PCR; Respiratory viruses, human Metapneumovirus (hMPV) PCR only CPT Codes: 87798 – Amplified probe technique, each organism Test Includes: Detection of human metapneumovirus in patients exhibiting symptoms of acute upper and /or lower respiratory tract infections by RT-PCR (Reverse Transcription Polymerase Chain Reaction. Logistics Lab Testing Sections: Sendout Outs – Microbiology/Virology Phone Number: MIN Lab: 612-813-5866 STP Lab: 651-220-6655 Referred to: Mayo Medical Laboratories (FHMPV) and forwarded to Focus Diagnostics (49200) Test Availability: Specimens accepted daily, 24 hours Turnaround Time: 1 – 5 days Special Instructions: Requisition must state specific site of specimen and date/time of collection. Specimen Specimen Type: Bronchoalveolar lavage (BAL) specimens; nasopharyngeal aspirates; NP swabs (V-C-M medium green cap or equivalent UTM) in 3 mL M4 media. Container: Sterile screw cap container; swab transport media; viral transport media Volume: 0.7 mL nasal aspirates or BAL; NP swabs Collection: Nasal Aspiration 1. Prepare suction set up on low to medium suction. 2. Wash hands. 3. Put on protective barriers (e.g., gloves, gown, mask). 4. Place child supine and obtain assistant to hold child during procedure. 5. Attach luki tube to suction tubing and #6 French suction catheter. 6. Insert catheter into nostril and pharynx without applying suction. 7. Apply suction as catheter is withdrawn. 8. If necessary, suction 0.5 – 1 mL of normal saline through catheter in order to clear the catheter and increase the amount of specimen in the luki tube. -
Unilateral Hyperhidrosis Associated with Underlying Intrathoracic Neoplasia
Thorax: first published as 10.1136/thx.41.10.814 on 1 October 1986. Downloaded from Thorax 1986;41:814-815 Unilateral hyperhidrosis associated with underlying intrathoracic neoplasia D C LINDSAY, J G FREEMAN, C 0 RECORD From the Department ofMedicine, Royal Victoria Infirmary, and University ofNewcastle upon Tyne Intrathoracic neoplasia is notable for the many ways in wall. There were metastatic plaques in the right hemithorax which it may present. We would like to report two cases and abdominal cavity but no evidence of metastases in the demonstrating a rare association between unilateral local- brain or the spinal cord. ised hyperhidrosis of the thoracic cage and underlying intra- thoracic neoplasm. Discussion Case reports The association of intrathoracic malignancy with sym- pathetic neurological complications, especially Homer's CASE 1 syndrome, is well recognised, particularly in the case of A 67 year old retired shotblaster complained ofa 3 kg weight tumours occurring at the thoracic inlet. Unilateral hyper- loss, mild dyspnoea, chest pain localised to the right costal hidrosis is an unusual phenomenon which has been reported margin, and profuse sweating localised to an area below the sporadically in association with various conditions, includ- right scapula. He smoked 15 cigarettes per day. Examination ing intracranial malignancy, encephalitis, syringomyelia, confirmed a right sided localised band of sweating at the trauma, neuritis, cervical rib, osteoma of the dorsal spine, level ofT6-9 posteriorly. Apart from minimal winging ofthe and chickenpox; in several cases no obvious underlying right scapula and some wasting of the right suprascapular cause has been evident. muscles no abnormal neurological signs were detected. -
Unraveling the Complexity of Chronic Pain and Fatigue
UNRAVELING THE COMPLEXITY OF CHRONIC PAIN AND FATIGUE LUCINDA BATEMAN, MD & BRAYDEN YELLMAN, MD © UNIVERSITY OF UTAH HEALTH SESSION #3 Effective use of evidence-based clinical diagnostic criteria and symptom management approaches to improve patient outcomes © UNIVERSITY OF UTAH HEALTH THE RATIONALE FOR USING EVIDENCE-BASED CLINICAL DIAGNOSTIC CRITERIA • Widespread pain amplification disorders – 1990 ACR fibromyalgia – 2016 ACR fibromyalgia criteria • Orthostatic Intolerance Disorders – POTS, NMH, OH, CAN, NOH… • ME/CFS 2015 IOM/NAM criteria © UNIVERSITY OF UTAH HEALTH PAIN AMPLIFICATION DISORDERS EX: FIBROMYALGIA ACR 1990 Chronic (>3 months) Widespread Pain (pain in 4 quadrants of body & spine) and Tenderness (>11/18 tender points) PAIN= stiffness, achiness, sharp shooting pains…tingling and numbness…light and sound sensitivity…in muscles, joints, bowel, bladder, pelvis, chest, head… FATIGUE, COGNITIVE and SLEEP disturbances are described in Wolfe et al but were not required for dx. Wolfe F, et al. The American College of Rheumatology 1990 criteria for the classification of fibromyalgia: report of the Multicenter Criteria Committee. Arthritis Rheum 1990;33:160–72 © UNIVERSITY OF UTAH HEALTH FIBROMYALGIA 1990 ACR CRITERIA Pain in four quadrants and the spine © UNIVERSITY OF UTAH HEALTH FIBROMYALGIA 2016 ACR CRITERIA 2016 Revisions to the 2010/2011 fibromyalgia diagnostic criteria, Seminars in Arthritis and Rheumatism. Volume 46, Issue 3. www.semarthritisrheumatism.com/article/S0049-0172(16)30208-6 © UNIVERSITY OF UTAH HEALTH FM IS OFTEN FOUND COMORBID WITH OTHER CONDITIONS Examples of the prevalence of fibromyalgia by 1990 criteria among various groups: General population 2% Women 4% Healthy Men 0.1% IM & Rheum clinics 15% IBS 13% Hemodialysis 6% Type 2 diabetes 15-23% Prevalence of fibromyalgia and co-morbid bipolar disorder: A systematic review and meta-analysis. -
The Influence of Temperature and Rainfall in the Human Viral Infections a Influência Da Temperatura E Índice Pluviométrico Nas Infecções Virais Humanas
Volume 1, Número 1, 2019 The influence of temperature and rainfall in the human viral infections A influência da temperatura e índice pluviométrico nas infecções virais humanas VIDAL, L. R. R.1*; PEREIra, L. A.1; DEBUR, M. C.2; RABONI, S. M.1,3; NOGUEIra, M. B.1,4; CavaLLI B.1; ALMEIDA, S. M.1,5 1 Virology Laboratory, Hospital de Clínicas da Universidade Federal do Paraná; 2 Molecular Biology Laboratory, Laboratório Central do Estado, Secretaria de Saúde do Estado do Paraná, 3 Departamento de Saúde Comunitária and Programa de Pós Graduação em Medicina Interna Infectious Diseases Department, Universidade Federal do Paraná; 4 Departamento de Análises Clínicas and Programa de Pós Graduação em Tocoginecologia, Universidade Federal do Paraná; *Corresponding Author: Luine Rosele Renaud Vidal Virology Laboratory, Setor de Ciências da Saúde, UFPR, Rua Padre Camargo, 280. Bairro Alto da Glória, CEP: 80.060-240 Phone number: +55 41 3360 7974 | E-mail: [email protected] DOI: https://doi.org/10.29327/226760.1.1-2 Recebido em 11/01/2019; Aceito em 15/01/19 Abstract It is well described by many authors the occurrence of viruses outbreaks that occurs annually at the same time. Epidemiological studies could generate important information about seasonality and its relation to outbreaks, population fluctuations, geographical variations, seasonal environmental. The purpose of this study was to show the influence of temperature and rainfall on the main viral diseases responsible for high rates of morbidity and mortality. Data were collected from samples performed at the Virology Laboratory of the Hospital de Clínicas da Universidade Federal do Paraná (HC-UFPR), which is a university tertiary hospital, in the period of 2003 to 2013. -
Measles Diagnostic Tool
Measles Prodrome and Clinical evolution E Fever (mild to moderate) E Cough E Coryza E Conjunctivitis E Fever spikes as high as 105ºF Koplik’s spots Koplik’s Spots E E Viral enanthem of measles Rash E Erythematous, maculopapular rash which begins on typically starting 1-2 days before the face (often at hairline and behind ears) then spreads to neck/ the rash. Appearance is similar to “grains of salt on a wet background” upper trunk and then to lower trunk and extremities. Evolution and may become less visible as the of rash 1-3 days. Palms and soles rarely involved. maculopapular rash develops. Rash INCUBATION PERIOD Fever, STARTS on face (hairline & cough/coryza/conjunctivitis behind ears), spreads to trunk, Average 8-12 days from exposure to onset (sensitivity to light) and then to thighs/ feet of prodrome symptoms 0 (average interval between exposure to onset rash 14 day [range 7-21 days]) -4 -3 -2 -1 1234 NOT INFECTIOUS higher fever (103°-104°) during this period rash fades in same sequence it appears INFECTIOUS 4 days before rash and 4 days after rash Not Measles Rubella Varicella cervical lymphadenopathy. Highly variable but (Aka German Measles) (Aka Chickenpox) Rash E often maculopapular with Clinical manifestations E Clinical manifestations E Generally mild illness with low- Mild prodrome of fever and malaise multiforme-like lesions and grade fever, malaise, and lymph- may occur one to two days before may resemble scarlet fever. adenopathy (commonly post- rash. Possible low-grade fever. Rash often associated with painful edema hands and feet. auricular and sub-occipital). -
E517.Full.Pdf
Life-Threatening Human Metapneumovirus Pneumonia Requiring Extracorporeal Membrane Oxygenation in a Preterm Infant Rolando Ulloa-Gutierrez, MD*; Peter Skippen, FRCPC‡; Anne Synnes, MDCM, MHSc§; Michael Seear, MD‡; Nathalie Bastien, PhD; Yan Li, PhD; and John C Forbes, MBChB* ABSTRACT. We present the first report in the literature the previous 12 hours, he had developed poor feeding, lethargy, of a child with human metapneumovirus pneumonia increasing cough, respiratory distress, and cyanosis. No history of who required extracorporeal membrane oxygenation for fever was documented. The patient’s father and 2 young siblings survival. This was a 3-month-old premature boy from each had experienced an uncomplicated, brief, nonfebrile, upper respiratory tract infection in the previous 3 weeks. British Columbia, Canada, who developed severe respi- This infant was born at 27 weeks of gestation (through cesarean ratory failure, experienced failure of high-frequency os- section, because of preterm labor), with a birth weight of 1200 g cillatory mechanical ventilation, and required extracor- and Apgar scores of 1 and 8 at 1 and 5 minutes, respectively. He poreal membrane oxygenation support for 10 days. This developed hyaline membrane disease and had a persistently case illustrates the importance of including this newly patent ductus arteriosus, which was treated with indomethacin. discovered pathogen among the causes of childhood Mechanical ventilation was required for 3 weeks. The patient was pneumonia. Pediatrics 2004;114:e517–e519. URL: www. discharged from the hospital at 2 months of age. Palivizumab was pediatrics.org/cgi/doi/10.1542/peds.2004-0345; human administered at 1 and 2 months of age, and the patient was metapneumovirus, viral pneumonia, prematurity, respira- scheduled to receive a third dose when he became ill. -
Human Metapneumovirus Circulation in the United States, 2008 to 2014 Amber K
Human Metapneumovirus Circulation in the United States, 2008 to 2014 Amber K. Haynes, MPH, a Ashley L. Fowlkes, MPH, b Eileen Schneider, MD,a Jeffry D. Mutuc, MPH,a Gregory L. Armstrong, MD, c Susan I. Gerber, MDa BACKGROUND: Human metapneumovirus (HMPV) infection causes respiratory illness, including abstract bronchiolitis and pneumonia. However, national HMPV seasonality, as it compares with respiratory syncytial virus (RSV) and influenza seasonality patterns, has not been well described. METHODS: Hospital and clinical laboratories reported weekly aggregates of specimens tested and positive detections for HMPV, RSV, and influenza to the National Respiratory and Enteric Virus Surveillance System from 2008 to 2014. A season was defined as consecutive weeks with ≥3% positivity for HMPV and ≥10% positivity for RSV and influenza during a surveillance year (June through July). For each virus, the season, onset, offset, duration, peak, and 6-season medians were calculated. RESULTS: Among consistently reporting laboratories, 33 583 (3.6%) specimens were positive for HMPV, 281 581 (15.3%) for RSV, and 401 342 (18.2%) for influenza. Annually, 6 distinct HMPV seasons occurred from 2008 to 2014, with onsets ranging from November to February and offsets from April to July. Based on the 6-season medians, RSV, influenza, and HMPV onsets occurred sequentially and season durations were similar at 21 to 22 weeks. HMPV demonstrated a unique biennial pattern of early and late seasonal onsets. RSV seasons (onset, offset, peak) were most consistent and occurred before HMPV seasons. There were no consistent patterns between HMPV and influenza circulations. CONCLUSIONS: HMPV circulation begins in winter and lasts until spring and demonstrates distinct seasons each year, with the onset beginning after that of RSV. -
Metapneumovirus
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Erasmus University Digital Repository Metapneumovirus determinants of host range and replication Miranda de Graaf ISBN: 978-90-9023746-6 The research described in this thesis was conducted at the Department of Virology of Erasmus MC, Rotterdam, The Netherlands, with financial support from the framework five grant “Hammocs” from the European Union and MedImmune Vaccines, USA. Printing of this thesis was financially supported by: Vironovative B.V., Viroclinics B.V., Greiner Bio-One. Cover art by Rosanne van der Meer and Miranda de Graaf Cartoons by Dirk-Jan de Graaf (p 61 and 143) Layout design by Aukje van Meeteren Printed by PrintPartners Ipskamp B.V Metapneumovirus determinants of host range and replication Metapneumovirus determinanten van gastheerspecificiteit en replicatie Proefschrift ter verkrijging van de graad van doctor aan de Erasmus Universiteit Rotterdam op gezag van de rector magnificus Prof.dr. S.W.J. Lamberts en volgens besluit van het College voor Promoties. De openbare verdediging zal plaatsvinden op donderdag 15 januari 2009 om 13.30 uur door: Miranda de Graaf geboren te Bergen (N.H.) Promotiecommissie Promotoren: Prof.dr. R.A.M. Fouchier Prof.dr. A.D.M.E. Osterhaus Overige leden: Prof.dr. A. van Belkum Prof.dr. M.P.G. Koopmans Prof.dr. B.K. Rima CONTENTS Page Chapter 1. General Introduction 1 Chapter 2. Recovery of human metapneumovirus genetic lineages A and B from 17 cloned cDNA Journal of Virology, 2004 Chapter 3. An improved plaque reduction virus neutralization assay for human 33 metapneumovirus Journal of Virological Methods, 2007 Chapter 4. -
Human Metapneumovirus Infection in Adults As the Differential Diagnosis of COVID-19
Turk J Intensive Care DOI: 10.4274/tybd.galenos.2020.62207 CASE REPORT / OLGU SUNUMU Lerzan Doğan, Human Metapneumovirus Infection in Adults as the Canan Akıncı, Zeynep Tuğçe Sarıkaya, Differential Diagnosis of COVID-19 Hande Simten Demirel Kaya, Rehile Zengin, COVID-19 Ayırıcı Tanısı Olarak Yetişkinlerde İnsan Orkhan Mammadov, Aylin İlksoz, Metapnömovirüs Enfeksiyonu İlkay Kısa Özdemir, Meltem Yonca Eren, Nazire Afsar, Sesin Kocagöz, ABSTRACT Human metapneumovirus (HMPV) is a respiratory tract virus identified 18 years prior İbrahim Özkan Akıncı to SARS-CoV-2. Both viruses cause acute respiratory failure characterised by a rapid onset of widespread inflammation in the lungs with clinical symptoms similar to those reported for other viral respiratory lung infections. HMPV, more generally known as childhood viral infection, causes Received/Geliş Tarihi : 13.11.2020 mild and self-limiting infections in the majority of adults, but clinical courses can be complicated Accepted/Kabul Tarihi : 07.12.2020 in risky groups and associated morbidity and mortality are considerable. Moreover, adults are not regularly screened for HMPV and the prevalence of adult HMPV infections in Turkey is unknown, with previous reports in the paediatric population. This should always be kept in mind during the COVID-19 pandemic, particularly when neurological complications are added to respiratory findings. In our study, two adult cases of HMPV pneumonia and encephalitis have been recorded. Lerzan Doğan, Zeynep Tuğçe Sarıkaya, Hande Simten Keywords: Acute respiratory infections, neurological involvement, wide respiratory screening Demirel Kaya, Orkhan Mammadov, Aylin İlksoz, İlkay Kısa Özdemir, İbrahim Özkan Akıncı ÖZ İnsan metapnömovirus (HMPV), SARS-CoV-2’den 18 yıl önce tanımlanan yeni solunum yolu Acıbadem Altunizade Hospital, Clinic of General virüsüdür. -
A Report of Two Cases of Human Metapneumovirus Infection in Pregnancy Involving Superimposed Bacterial Pneumonia and Severe Respiratory Illness
Case Report J Clin Gynecol Obstet. 2019;8(4):107-110 A Report of Two Cases of Human Metapneumovirus Infection in Pregnancy Involving Superimposed Bacterial Pneumonia and Severe Respiratory Illness Jordan P. Emonta, c, Kathleen S. Chunga, Dwight J. Rousea, b Abstract tion (URI) [2]. In a literature search on PubMed of “human metapneu- Human metapneumovirus (HMPV) is a cause of mild to severe res- movirus AND pregnant” and “human metapneumovirus AND piratory viral infection. There are few descriptions of infection with pregnancy”, we identified two case reports of severe HMPV HMPV in pregnancy. We present two cases of HMPV infection occur- infection in pregnant women in the USA, and one descrip- ring in pregnancy, including a case of superimposed bacterial pneu- tive report of 25 pregnant women infected with mild HMPV monia in a pregnant woman after HMPV infection. In the first case, infection in rural Nepal. In a case by Haas et al (2012), a a 40-year-old woman at 29 weeks of gestation developed an asthma 24-year-old woman at 30 weeks of gestation developed res- exacerbation in association with a positive respiratory pathogen panel piratory failure requiring intensive care unit (ICU) admission (RPP) for HMPV infection. She was admitted to the intensive care secondary to HMPV pneumonia [3]. The case by Fuchs et al unit (ICU) for progressive respiratory failure. In the second case, a (2017) describes an 18-year-old patient at 36 weeks of gesta- 36-year-old woman at 31 weeks of gestation developed respiratory tion admitted to an intensive care unit (ICU) for acute respira- distress in association with a positive RPP for HMPV. -
Botox® Reconstitution and Dilution Procedures
BOTOX® RECONSTITUTION AND DILUTION PROCEDURES Indication Chronic Migraine BOTOX® (onabotulinumtoxinA)for injection is indicated for the prophylaxis of headaches in adult patients with chronic migraine (≥ 15 days per month with headache lasting 4 hours a day or longer). Important Limitations Safety and effectiveness have not been established for the prophylaxis of episodic migraine (14 headache days or fewer per month) in 7 placebo-controlled studies. IMPORTANT SAFETY INFORMATION, INCLUDING BOXED WARNING WARNING: DISTANT SPREAD OF TOXIN EFFECT Postmarketing reports indicate that the effects of BOTOX® and all botulinum toxin products may spread from the area of injection to produce symptoms consistent with botulinum toxin effects. These may include asthenia, generalized muscle weakness, diplopia, ptosis, dysphagia, dysphonia, dysarthria, urinary incontinence, and breathing difficulties. These symptoms have been reported hours to weeks after injection. Swallowing and breathing difficulties can be life threatening, and there have been reports of death. The risk of symptoms is probably greatest in children treated for spasticity, but symptoms can also occur in adults treated for spasticity and other conditions, particularly in those patients who have an underlying condition that would predispose them to these symptoms. In unapproved uses, including spasticity in children, and in approved indications, cases of spread of effect have been reported at doses comparable to those used to treat cervical dystonia and upper limb spasticity and at lower -
Ah-Choo! Is It a Cold, Hay Fever, Sinusitis Or the Flu?
Upper Respiratory Tract Infection Comparison Chart Ah-choo! Is it a Cold, Hay Fever, Sinusitis or the Flu? Name Symptoms Causes Onset & duration of Prevention Treatment symptoms Common Cold • No fever More than 200 different viruses, • Usually comes on gradually Frequent hand washing • Bed rest & plenty of fluids (“Head Cold”) • No aches and pains including: • Most common Sept-April • Acetaminophen or aspirin for • Still have an appetite • Rhinovirus (nose virus) • Adults will get 2-4 colds/year headache/ fever • Sore throat • RSV (respiratory syncytial • Children will get up to 12 • See a physician if cold lasts • Runny, stuffy nose virus) colds/year more than 10 days • Sneezing • Corona viruses • Colds are a powerful asthma • Most prominent symptoms trigger are in the nose • Lasts about one week Flu • Sudden fever, chills Viruses, spread by: • Usually comes on suddenly • Highly recommended to get Most people recover without (Influenza) • Aching muscles and joints • Sneezed or coughed droplets • Lasts about a week a flu shot to prevent the flu, treatment • Headache into the air from an infected especially if history of • Severe malaise person asthma, recurrent ear • Dry cough & lack of appetite • Cold & dry weather, as people infections, and sinusitis. • Blocked and/or runny nose spend more time close together • Best time for flu shot is Oct - • Your ” whole body” feels sick indoors. Nov Allergic Rhinitis • No fever Exposure to irritants/triggers: • Weeks, months or all year. Avoid triggers; for example: • Antihistamines (“Hay Fever”) • Congestion • Dust mites • Symptoms last as long as • Remove carpeting to reduce • Intranasal steroids • Runny or stuffy nose (clear, • Animal dander you are exposed to the dust mites and mould • Immunotherapy may help white thin mucus) • Pollen allergen.