D127219 Anti-IL1RL2 antibody

Cat. No. D127219 Package 25 μl/100 μl/200 μl Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Product overview

Description A n ti-IL1RL2 rabbit polyclonal antibody Applications ELISA, WB, IHC Immunogen Fusion of human IL1RL2 Reactivity Human Content 1 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification

Target information

Symbol IL1RL2 Full name 1 receptor like 2 Synonyms IL-36R; IL1RRP2; IL-1Rrp2; IL1R-rp2 Swissprot Q9HB29

Target Background

The protein encoded by this is a member of the interleukin 1 receptor family. An experiment with transient gene expression demonstrated that this receptor was incapable of binding to interleukin 1 alpha and interleukin 1 beta with high affinity. This gene and four other interleukin 1 receptor family , including interleukin 1 receptor, type I (IL1R1), interleukin 1 receptor, type II (IL1R2), interleukin 1 receptor-like 1 (IL1RL1), and receptor 1 (IL18R1), form a gene cluster in a region mapped to 2q12. [provided by RefSeq, Jul 2008] Sangon Biotech

Applications

Immunohistochemistry Predicted cell location: Cytoplasm Predicted cell location: Cytoplasm Positive control: Human prostate cancer Positive control: Human ovarian cancer Recommended dilution: 100-300 Recommended dilution: 100-300

The image on the left is immunohistochemistry of paraffin-embedded The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using D127219(IL1RL2 Antibody) at Human ovarian cancer tissue using D127219(IL1RL2 Antibody) at dilution 1/130, on the right is treated with fusion protein. (Original dilution 1/130, on the right is treated with fusion protein. (Original magnification: ×200) magnification: ×200)

Western blotting Predicted band size:65 kDa Positive control:K562 cell lysate Recommended dilution: 500-2000

Gel: 8%SDS-PAGE Lysate: 40 μg Lane: K562 cell lysate Primary antibody: D127219(IL1RL2 Antibody) at dilution 1/1000 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 20 seconds

ELISA Sangon Biotech Recommended dilution: 5000-10000