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Journal of Antimicrobial Agents., 1998, 10, 67–75

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Journal of Antimicrobial Agents., 1998, 10, 67–75 ISSN 1314-9091 1 PP R O C E E D I N G S OF THE FOURTH WORKSHOP ON EXPERIMENTAL MODELS AND METHODS IN BIOMEDICAL RESEARCH May 27 – 29, 2013 Institute of Experimental Morphology, Pathology and Anthropology with Museum at the Bulgarian Academy of Sciences Edited by: Dimitar Kadiysky and Radostina Alexandrova Supported by: National Science Fund, Ministry of Education and Science, Bulgaria – Grant DTK 02 70/2009; European Social Fund and Republic of Bulgaria, Operational Programme “Human Resources Development” 2007-2013 Framework, Grant № BG051PO001-3.3.06-0048 from 04.10.2012; Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences 2 THE FORTH WORKSHOP “EXPERIMENTAL MODELS AND METHODS IN BIOMEDICAL RESEARCH” IS ORGANIZED BY THE INSTITUTE OF EXPERIMENTAL MORPHOLOGY, PATHOLOGY AND ANTHROPOLOGY WITH MUSEUM (IEMPAM) UNDER THE AUSPICES OF THE BULGARIAN ACADEMY OF SCIENCES ORGANIZING COMMITTEE CHAIRPERSON: SECRETARY: NINA ATANASSOVA (IEMPAM – BAS) RADOSTINA ALEXANDROVA (IEMPAM – BAS) MEMBERS: DIMITAR KADIYSKY (IEMPAM – BAS) MARIN ALEXANDROV (IEMPAM – BAS) IVAN IVANOV (IEMPAM – BAS) CO-ORGANIZERS RENI KALFIN (INSTITUTE OF NEUROBIOLOGY, BULGARIAN ACADEMY OF SCIENCES) GEORGE MILOSHEV (INSTITUTE OF MOLECULAR BIOLOGY, BULGARIAN ACADEMY OF SCIENCES) MARIA NIKOLOVA (NATIONAL CENTRE OF INFECTIOUS AND PARASITIC DISEASES, SOFIA) KONSTANTA TIMCHEVA (NATIONAL SPECIALIZED HOSPITAL FOR ACTIVE TREATMENT IN ONCOLOGY, SOFIA) MARIELA ODJAKOVA (FACULTY OF BIOLOGY, SOFIA UNIVERSITY “KLIMENT OHRIDSKI”) TSVETANKA MARINOVA (FACULTY OFMEDICINE, SOFIA UNIVERSITY “KLIMENT OHRIDSKI”) ANNA TOLEKOVA (MEDICAL FACULTY, TRAKIA UNIVERSITY, STARA ZAGORA) BORYANA RUSEVA (MEDICAL UNIVERSITY, PLEVEN) 3 YOUNG SCIENTISTS COMMITTEE DELKA SALKOVA (IEMPAM – BAS) IVAN ILIEV (IEMPAM - BAS) VASIL ATANASOV (FACULTY OF CHEMISTRY AND PHARMACY, UNIVERSITY OF SOFIA “ST. KLIMENT OHRIDSKI”; MILITARY MEDICAL ACADEMY - SOFIA)) MILENA GEORGIEVA (INSTITUTE OF MOLECULAR BIOLOGY - BAS) DRAGOMIR DARDANOV (FACULTY OF MEDICINE, SOFIA UNIVERSITY “KLIMENT OHRIDSKI”, 5 CITY HOSPITAL - SOFIA) IVA GAVRILOVA (NATIONAL SPECIALIZED HOSPITAL FOR ACTIVE TREATMENT IN ONCOLOGY, SOFIA) TANIA ZHIVKOVA (IEMPAM – BAS) BOYKA ANDONOVA-LILOVA (IEMPAM – BAS) LORA DYAKOVA (INSTITUTE OF NEUROBIOLOGY – BAS) IVELIN VLADOV (IEMPAM – BAS) ABEDULKADIR ABUDALLEH (IEMPAM – BAS, FACULTY OF BIOLOGY, SOFIA UNIVERSITY “KLIMENT OHRIDSKI”) TIM VLADIMIROV (FACULTY OF MEDICINE, SOFIA UNIVERSITY “KLIMENT OHRIDSKI”) INTERNATIONAL ADVISORY BOARD VLADIMIR KULCHITSKY (INSTITUTE OF PHYSIOLOGY, NATIONAL ACADEMY OF SCIENCES – BELARUS) OTILIA COSTISOR (INSTITUTE OF CHEMISTRY, ROMANIAN ACADEMY, TIMISOARA, ROMANIA) LUMINITA PATRON (INSTITUTE OF PHYSICAL CHEMISTRY, ROMANIAN ACADEMY, BUCHAREST, ROMANIA) GEORGETA MARIA SIMU (FACULTY OF PHARMACY, UNIVERSITY OF MEDICINE AND PHARMACY, TIMISOARA, ROMANIA) MILAN CIZ (INSTITUTE OF BIOPHYSICS, ACADEMY OF SCIENCES OF THE CZECH REPUBLIC) ANTONIN LOJEK (INSTITUTE OF BIOPHYSICS, ACADEMY OF SCIENCES OF THE CZECH REPUBLIC) DARINA LAZAROVA (THE COMMONWEALTH MEDICAL COLLEGE, SCRANTON, PA, USA) MICHAEL BORDONARO (THE COMMONWEALTH MEDICAL COLLEGE, SCRANTON, PA, USA) 4 P R O G R A M M E Monday, 27 May 2013 9.00-9.45 - Registration 9.45 – Opening Ceremony Session A. Chairpersons: Assoc. Prof. Evelina Shikova, MD, PhD Assoc. Prof. Russy Russev, DVM, PhD Secretary: Lora Dyakova 10.00-10.20 AO1. THE RETROVIRUSES THAT CHANGED THE WORLD OF SCIENCE Radostina Alexandrova 10.20 – 10.40 AO2. AURORA PROJECT: A EUROPEAN NETWORK ON CERVICAL CANCER SURVEILLANCE AND CONTROL IN THE NEW MEMBER STATES E. Shikova I. Ivanov, G. Ganchev, D. Mircheva 10.40 – 10.50 AO3. HPV DNA TESTING FOR IDENTIFICATION OF HPV INFECTION E. Shikova and Z. Ivanova 10.50 – 11.10 Coffee Break 11.10-11.30 AO4. PAPILLOMAVIRUS-LIKE PARTICLES FOR HPV VACCINES DEVELOPMENT E. Shikova, Z. Ivanova 5 11.30 – 11.50 AO5 BLV INFECTED RATS AND RABBITS AS A MODEL OF HUMAN LYMPHOCYTIC LEUKAEMIA Petar Dimitrov, Katerina Todorova, Rositca Milcheva, Evgeny Gabev and Russy Russev 11.50 – 12.10 AO6. PLANT SUBSTANCES ARE PROMISING INHIBITORS OF HERPES VIRUS INFECTIONS Neli Milenova Vilhelmova-Ilieva 12.10 – 12.20 AO7. BIOLOGICAL PROPERTIES OF ELLAGITANNINS IMPORTANT FOR HUMAN HEALTH Neli Milenova Vilhelmova-Ilieva 12.20 – 12.30 POSTER SESSION A AP1. ВИРУСИ - ЛЕКАРСТВО ЗА МОЗЪЧНИ ТУМОРИ Тим Владимиров Владимиров 12.30 – 12.35 AP2. БАКТЕРИОФАГИТЕ – АНТИБИОТИЦИ НА БЪДЕЩЕТО Яна Славейкова 12.30 – 13.30 LUNCH TIME 13.30 – 14.00 ПРЕДСТАВЯНЕ НА НОВИТЕ ПРОГРАМИ И СТИПЕНДИИ НА НЕМСКАТА ФОНДАЦИЯ "АЛЕКСАНДЪР ФОН ХУМБОЛДТ" проф. Илза Пъжева 6 Session B. Chairpersons: Prof. Reneta Toshkova, MD, PhD Assoc. Prof. Svetlozara Petkova, PhD Secretary: Assist. Prof. Petar Dimitrov, DVM, PhD 14.10 – 14.30 BO1. INVESTIGATIONS ON SOME HEMATOLOGICAL PARAMETERS IN SHEEP EXPERIMENTALLY INFECTED WITH HAEMONCHUS CONTORTUS V. Nanev, I. Vladov, D. Hrusanov, M. Gabrashanska 14.30-14.40 BO2. MODEL OF EIMERIA TENELLA INFECTION AFTER BASIC COPPER SALT SUPPLEMENTATION M. Anisssimova, I.Vladov, V. Koinaski, M. Gabrashanska, V. Ermakov, S. Tyutikov 14.40 – 14.50 BO3. COMPARISON OF ALLOZYME ANALYSIS OF SIX TRICHINELLA ISOLATES BY PAAE Valeria Dilcheva, Svetlozara Petkova, Ivan Iliev 14.50 – 15.10 BO4. SERUM SIALIC ACID LEVELS IN TRICHINELLA SPIRALIS INFECTED RATS D. Ivanov, M. Gabrashanska, R. Gavazova, S. Petkova 15.10 – 15.30 Coffee Brfeak 15.30 – 15.50 BO5. THE NURSE CELL – TRICHINELLA SPIRALIS COMPLEX AS AN EXPERIMENTAL MODEL FOR INVESTIGATIONS ON THE GLYCOSYLATION IN SKELETAL MUSCLE TISSUE R. Milcheva, B. Izrael-Vlková, D. Ivanov, S. Petkova, P. Celec, P. Janega, R. Gavazova, R. Russev, P. Babál 7 15.50 – 16.10 BO6. EFFECT OF BIOLOGICALLY ACTIVE SUBSTANCES ISOLATED UNDER FASCIOLOSIS ON LYMPHOCYTE CELL CULTURES Tsocheva-Gaytandzhieva, N., E. Nikolova, D. Salkova 16.10 – 16.20 BO7. EFFECT OF THERMOLABILE BIOLOGICALLY ACTIVE SUBSTANCES UNDER FASCIOLOSIS ON TUMOR CELL CULTURE N. Tsocheva-Gaytandzhieva, R. Toshkova, A. Filchev, D. Salkova 16.20 – 16.40 BO8. NOSEMOSIS – THE PESTS OF THE 21th CENTURY IN EUROPE Delka Salkova Salkova 16.40 - 17.00 POSTER PRESENTATION - SESSION B BP1. THE DARK SIDE OF TOXOPLASMA GONDII Deyana Jekova ВР2. ENTAMOEBA HISTOLYTICA Кристина Антонова Генкова ВР3. КУЧЕТО - ПРЯТЕЛ ИЛИ ВРАГ НА ЧОВЕКА. КУЧЕШКА ТЕНИЯ Кристин Ботева ВР4. ECHINOCOCCUS GRANULOSUS Мартин Григоров, Ради Фетиани, Тамара Саад ВР5. ВНИМАНИЕ: ТЕНИИ! Венета Димитрова , Ива Политова, Стефка Георгиева ВР6. SCHISTOSOMA Мартин Григоров, Тамаара Саад, Ради Фетияни 8 Tuesday, 28 May 2013 Session C. Chairpersons: Prof. Elena Nikolova, PhD, DSc Assoc. Anton Kril, DVM, PhD Secretary: Tanya Zhivkova 9.45 – 10.05 CO1. EFFECTS OF LEAD AND SALINOMYCIN ON SOME ORGAN WEIGHT/BODY WEIGHT INDICES IN FEMALE MICE, SUBJECTED TO SUBACUTE LEAD INTOXICATION Juliana Ivanova, Yordanka Gluhcheva, Silvia Stoykova, Vasil Atanasov, Sonja Arpadjan 10.05 – 10.15 CO2. CHANGES IN MICE SPERM COUNT AFTER SUBACUTE INTOXICATION WITH LEAD AND SALINOMYCIN DETOXICATION - PRELIMINARY DATA Ekaterina Pavlova, Donika Dimova, Juliana Ivanova, Yordanka Gluhcheva, Nina Atanassova 10.15 – 10.35 CO3. IN OVO STUDY ON THE GENOTOXIC AND CARCINOGENIC POTENTIAL OF ANTHRACENE-DRIVED SCHIFF BASES AND ANTHRACENE-CONTAINING AMINOPHOSPHONATES A. Kril, A. Georgieva, I. Iliev, I. Tsacheva, E. Vodenicharova, E. Tashev, T. Tosheva, I. Kraicheva, K. Troev 10.35 – 10.55 CO4. CRITERIA FOR MECHANISM-BASED ENZYME INACTIVATION Tzveta Stoyanova, Viliana Todorova, Stanislav Yanev 10.55 – 11.05 CO5. ACYLPEPTIDE HYDROLASE AS A BIOMARKER OF EXPOSURE TO ORGANOPHOSPHATES Vassil Komsalov, Stanislav Yanev, Bozhidarka Pandova, Viliana Todorova 9 11.05 – 11.15 INCREASED TOXICITY OF NEWLY SYNTHESIZED PEPTIDOMIMETICS BY DIMETHYL SULPHOXIDE AS SOLVENT IN RODENTS Klisurov R., E. Encheva, L. Tancheva, M. Novoselski, D. Tsekova, M. Genadieva, Sv. Stoeva 11.15-11.20 POSTER PRESENTATION - SESSION C CP1. СИНТЕТИЧНА БИОЛОГИЯ: ПРИЛОЖЕНИЕ В МЕДИЦИНАТА Мария Минчева и Вивиан Петрова 11.20 – 11.40 Coffee Break Session D. Chairpersons: Prof. Margarita Gabrashanska, DVM, PhD Assoc. Prof. Diana Rabadjieva, PhD Secretary: Abedulkadir Mahdi Abudalleh 11.40 – 11.50 DO1. BIOCHEMICAL STUDIES IN RATS WITH EXPERIMENTAL DEFECTS FILLED WITH DIFFERENT HYDROXYAPATITE SUBSTITUTES M. Gabrashanska. R. Ilieva, M. Alexandrov, K.Sezanova, V. Nanev, I. Vladov 11.50 – 12.10 DO2. MURINE BONE MAROOW CELLS AND CULTURES FROM BONE EXPLANTS IN THE EVALUATION OF BIOCOMPATIBILITY OF CALCIUM PHOSPHATE MATERIALS FOR BONE IMPLANTS Radostina Alexandrova, Tanya Zhivkova, Boyka Andonova-Lilova, Lora Dyakova, Gergana Taleva, Diana Rabadjieva, Stefka Tepavitcharova 12.10-12.30 DO3. DEVELOPMENT OF NOVEL IN VITRO-CULTIVATION TECHNIQUES FOR DERIVATION OF CORNEAL EPITHELIAL EXPLANTS Ilina Valkova, Iskra Sainova,Ani Georgieva, Anatoli Neronov, Marin Bratanov, Elena Nikolova 10 12.30 – 13.30 Lunch Time 13.30 – 13.50 DO4. ПРОУЧВАНЕ ВЪРХУ ВЛИЯНИЕТО НА КРИОКОНСЕРВАЦИЯТА, ПО ОТНОШЕНИЕ ПОДВИЖНОСТТА И ДНК ФРАГМЕНТАЦИОННИЯ ИНДЕКС НА СПЕРМАТОЗОИДИТЕ, ПРИ СВЕЖА И ОБРАБОТЕНА СЕМЕННА ПРОБА Л. Илиева, Н. Айвазова, В. Рилчева, Д. Мартинов, Е. Конова 13.50- 14.10 DO5. ASSESSMENT OF FROZEN-THAWED HUMAN SPERM USING AN ANNEXIN V BINDING AND DNA INTEGRITY TEST BY FLOW CYTOMETRY V. Rilcheva, N. Ayvazova, L. Ilieva, Tz. Lukanov, E. Konova 14.10 – 14.20 POSTER PRESENTATION - SESSION D DP1. СТЕРИЛИТЕТЪТ ПРИ МЪЖЕТЕ – ПОГЛЕД ОТ ВСИЧКИ СТРАНИ Радостина Димитрова, Милица Симов DP2. IN VITRO MATURATION OF HUMAN OOCYTES Galina Nenkova DP3. МЕТОДИ ЗА ОПЛОЖДАНЕ IN VITRO Даниела Гулева, Александра Борисова, Николай Димитров Session
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