DOCSLIB.ORG

Sp. Nov., but Not Mycobacterium

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Sp. Nov., but Not Mycobacterium Washington University School of Medicine Digital Commons@Becker Open Access Publications 2016 Mycobacterium arupense, Mycobacterium heraklionense, and a newly proposed species, “Mycobacterium virginiense” sp. nov., but not Mycobacterium nonchromogenicum, as species of the Mycobacterium terrae complex causing tenosynovitis and osteomyelitis Ravikiran Vasireddy University of Texas Health Center at Tyler Sruthi Vasireddy University of Texas Health Center at Tyler Barbara A. Brown-Ellliott University of Texas Health Center at Tyler Nancy L. Wengenack Mayo Clinic Uzoamaka A. Eke Washington University School of Medicine in St. Louis Recommended Citation Vasireddy, Ravikiran; Vasireddy, Sruthi; Brown-Ellliott, Barbara A.; Wengenack, Nancy L.; Eke, Uzoamaka A.; Benwill, Jeana L.; Turenne, Christine; and Wallace, Richard J. Jr., ,"Mycobacterium arupense, Mycobacterium heraklionense, and a newly proposed species, “Mycobacterium virginiense” sp. nov., but not Mycobacterium nonchromogenicum, as species of the Mycobacterium terrae complex causing tenosynovitis and osteomyelitis." Journal of Clinical Microbiology.54,5. 1340-1351. (2016). https://digitalcommons.wustl.edu/open_access_pubs/4955 This Open Access Publication is brought to you for free and open access by Digital Commons@Becker. It has been accepted for inclusion in Open Access Publications by an authorized administrator of Digital Commons@Becker. For more information, please contact engeszer@wustl.edu. See next page for additional authors Follow this and additional works at: https://digitalcommons.wustl.edu/open_access_pubs Authors Ravikiran Vasireddy, Sruthi Vasireddy, Barbara A. Brown-Ellliott, Nancy L. Wengenack, Uzoamaka A. Eke, Jeana L. Benwill, Christine Turenne, and Richard J. Wallace Jr. This open access publication is available at Digital Commons@Becker: https://digitalcommons.wustl.edu/open_access_pubs/4955 crossmark Mycobacterium arupense, Mycobacterium heraklionense, and a Newly Proposed Species, “Mycobacterium virginiense” sp. nov., but Not Mycobacterium nonchromogenicum, as Species of the Mycobacterium terrae Complex Causing Tenosynovitis and Osteomyelitis Downloaded from Ravikiran Vasireddy,a Sruthi Vasireddy,a Barbara A. Brown-Elliott,a Nancy L. Wengenack,b Uzoamaka A. Eke,c* Jeana L. Benwill,a Christine Turenne,d* Richard J. Wallace, Jr.a Mycobacteria/Nocardia Research Laboratory, Department of Microbiology, The University of Texas Health Science Center at Tyler, Tyler, Texas, USAa; Mayo Clinic, Rochester, Minnesota, USAb; Division of Infectious Disease, Washington University in St. Louis School of Medicine, St. Louis, Missouri, USAc; Saskatchewan Disease Control Laboratory, Regina, Saskatchewan, Canadad Mycobacterium terrae complex has been recognized as a cause of tenosynovitis, with M. terrae and Mycobacterium nonchromo- genicum reported as the primary etiologic pathogens. The molecular taxonomy of the M. terrae complex causing tenosynovitis http://jcm.asm.org/ has not been established despite approximately 50 previously reported cases. We evaluated 26 isolates of the M. terrae complex associated with tenosynovitis or osteomyelitis recovered between 1984 and 2014 from 13 states, including 5 isolates reported in 1991 as M. nonchromogenicum by nonmolecular methods. The isolates belonged to three validated species, one new proposed species, and two novel related strains. The majority of isolates (20/26, or 77%) belonged to two recently described species: Myco- bacterium arupense (10 isolates, or 38%) and Mycobacterium heraklionense (10 isolates, or 38%). Three isolates (12%) had 100% sequence identity to each other by 16S rRNA and 99.3 to 100% identity by rpoB gene region V sequencing and represent a previ- ously undescribed species within the M. terrae complex. There were no isolates of M. terrae or M. nonchromogenicum, including among the five isolates reported in 1991. The 26 isolates were susceptible to clarithromycin (100%), rifabutin (100%), ethambu- on May 31, 2016 by Washington University in St. Louis tol (92%), and sulfamethoxazole or trimethoprim-sulfamethoxazole (70%). The current study suggests that M. arupense, M. heraklionense, and a newly proposed species (“M. virginiense” sp. nov.; proposed type strain MO-233 [DSM 100883, CIP 110918]) within the M. terrae complex are the major causes of tenosynovitis and osteomyelitis in the United States, with little change over 20 years. Species identification within this complex requires sequencing methods. ycobacterium terrae complex (MTC) was first characterized ture sequence for members of the MTC compared to other slowly Min 1981 by the International Working Group in Mycobacte- growing mycobacteria (37, 38). He also characterized several new rial Taxonomy (IWGMT). The initial MTC consisted of two non- species in the complex, including Mycobacterium heraklionense chromogenic slowly growing species: M. terrae and Mycobacte- and Mycobacterium engbaekii (1). rium nonchromogenicum (1, 2). Phenotypic separation within the The greater availability of DNA sequencing (59) has resulted in group was often difficult, and molecular methods were not avail- a “boom” of new species of MTC, beginning with Mycobacterium able, making establishment of species pathogenicity uncertain (3). hiberniae (39). An additional eight new species have been validly The complex is recognized as an environmental contaminant published since 2006: Mycobacterium arupense (28), Mycobacte- of sputum and a cause of tenosynovitis and osteomyelitis primar- rium kumamotonense (36), Mycobacterium heraklionense (1), My- ily of the fingers and wrist (3–35). Whether one or more members cobacterium senuense (40), Mycobacterium minnesotense (41), My- of the complex are true respiratory pathogens has not been estab- lished (1, 22). The first published case report of tenosynovitis caused by the MTC was by Hirata and Tomiyama in 1976 (4). There have been Received 28 January 2016 Returned for modification 16 February 2016 Accepted 1 March 2016 approximately 34 additional case reports published since then, Accepted manuscript posted online 9 March 2016 identified using nonmolecular methods (3–25), with 14 cases Citation Vasireddy R, Vasireddy S, Brown-Elliott BA, Wengenack NL, Eke UA, identified using molecular methods (26–28, 30–36)(Tables 1 and Benwill JL, Turenne C, Wallace RJ, Jr. 2016. Mycobacterium arupense, 2). With the exception of four isolates of M. arupense, including Mycobacterium heraklionense, and a newly proposed species, “Mycobacterium the original description of M. arupense (28), details of the methods virginiense” sp. nov., but not Mycobacterium nonchromogenicum, as species of the and/or explicitly stating a 100% 16S rRNA gene sequence identity Mycobacterium terrae complex causing tenosynovitis and osteomyelitis. J Clin Microbiol 54:1340–1351. doi:10.1128/JCM.00198-16. to recognized species for the remaining cases with molecular iden- Editor: G. A. Land tifications have been absent (Table 2). Address correspondence to Ravikiran Vasireddy, ravikiran.vasireddy@uthct.edu. An excellent history and species update of the M. terrae com- * Present address: Uzoamake A. Eke, Infectious Diseases Consultants of Detroit, plex based on multigene sequencing targets was published by Tor- Southfield, Michigan, USA; Christine Turenne, St. Boniface Hospital, Diagnostic toli et al. (1). He noted that the presence of a two nucleotide Services Manitoba, Winnipeg, MB, Canada. insertion in helix 18 of the 16S rRNA gene (bp ϳ430 to 500; Copyright © 2016, American Society for Microbiology. All Rights Reserved. hypervariable region B or region V3) provided a consistent signa- 1340 jcm.asm.org Journal of Clinical Microbiology May 2016 Volume 54 Number 5 Mycobacterium terrae Species Causing Tenosynovitis TABLE 1 Characteristics and species designations of 35 previously reported cases of tenosynovitis due to the M. terrae complex without molecular species identificationa Local Granulomatous AFB Location/yr of No. Age/sex Site of tenosynovitis Diagnosis or risk factor(s)s steroid Surgery inflammation smear Species Reference publication 1 65/M Right foot (ankle) Farmer; NA ϩ NA NA NA M. nonchromogenicum 4 Japan/1976 2 20/M Left forefinger, wrist NA ϩϩ ϩ ϩM. terrae 5 Texas/1978 3 23/M Left index finger Puncture from fish fin ϩϩ ϩ ϪM. terrae 6 NA/1979 4 57/M Knee RA ϩ NA NA ? M. terrae 7 NA/1981 5 66/F Right index finger Puncture from blackberry Ϫϩ ϩ ϩM. terrae 8 NA/1981 thorn Downloaded from 6 60/M Left hand, forearm Puncture from wooden Ϫϩ ϩ ϪM. terrae 9 NA/1983 splinter 7 47/F Right 4th and 5th Puncture from straight pin ϩϩ ϩ ϪM. terrae 10 NA/1983 fingers, palm, thumb 8 72/M left forearm, right 5th Puncture wound Ϫϩ ϩ ϪM. terrae 11 France/1984 finger, right thumb 9 75/M Left index finger Arteritis ϩϩ ϩ ϪM. terrae 12 NA/1985 10 NA Knee NA ϩ NA NA NA M. terrae 13 France/1987 11 54/M Right 3rd finger, PIP Puncture from fish fin ϩϩ ϩ ϩM. terrae 14 NA/1988 http://jcm.asm.org/ joint 12 55/M Right 3rd finger, Puncture from fish fin ϪϪ NA Ϫ M. terrae 14 NA/1988 MCP joint 13 41/F Hand, wrist Renal transplant ϩ NA NA NA M. terrae 15 NA/1988 14 58/F Right, 3rd finger Nurse ϩϩ ϩ ϪM. terrae 16 Sweden/1989 15 28/F Right, 2nd finger Lab technician Ϫϩ Ϫ ϪM. terrae 16 Sweden/1989 16 71/F Right, 2nd finger Retired ϩϩ Ϫ ϪM. terrae 16 Sweden/1989 17 47/F Right 3rd finger Cook ϩϩ ϩ ϪM. terrae 16 Sweden/1989 18 31/F Right, 2nd finger Office worker ϩϩ ϩ ϪM. terrae 16 Sweden/1989 19 38/F Right, 3rd finger Lab technician ϩϩ ϩ ϪM. terrae 16 Sweden/1989 on May 31, 2016 by Washington University in St. Louis 20 48/M 3rd finger NA ? ϩ ??M. terrae 17 NA/1989 21 63/F Wrist Gardener NA NA NA NA M. terrae 18 NA/1990 22 58/F Right index finger Gardening, cook (case 1) Ϫϩ ϩ ϪM. nonchromogenicum 3 Maine/1991 23 60/M Left wrist Meat cutter (case 2) Ϫϩ ϩ ϪM. nonchromogenicum 3 Illinois/1991 24 59/M Left wrist Maintenance work (case 3) Ϫϩ ϩ ϪM. nonchromogenicum 3 Florida/1991 25 62/F Right middle finger NA (case 4) Ϫϩ ϩ ϪM. nonchromogenicum 3 Texas/1991 26 40/M Left hand, thumb Dermatomyositis on ϩϩ ϩ ϪM. nonchromogenicum 3 Florida/1991 steroids (case 5) 27 40/F Right 5th finger Fishing, gardening, prior Ϫϩ ϩ NA M.
Load more

Recommended publications
  • Mycobacterium Arupense Among the Isolates of Non-Tuberculous Mycobacteria from Human, Animal and Environmental Samples
    Veterinarni Medicina, 55, 2010 (8): 369–376 Original Paper Mycobacterium arupense among the isolates of non-tuberculous mycobacteria from human, animal and environmental samples M. Slany1, J. Svobodova2, A. Ettlova3, I. Slana1, V. Mrlik1, I. Pavlik1 1Veterinary Research Institute, Brno, Czech Republic 2Regional Institute of Public Health, Brno, Czech Republic 3BioPlus, s.r.o., Brno, Czech Republic ABSTRACT: Mycobacterium arupense is a non-tuberculous, potentially pathogenic species rarely isolated from humans. The aim of the study was to ascertain the spectrum of non-tuberculous mycobacteria within 271 sequenced mycobacterial isolates not belonging to M. tuberculosis and M. avium complexes. Isolates were collected between 2004 and 2009 in the Czech Republic and were examined within the framework of ecological studies carried out in animal populations infected with mycobacteria. A total of thirty-three mycobacterial species were identified. This report describes the isolation of M. arupense from the sputum of three human patients and seven different animal and environmental samples collected in the last six years in the Czech Republic: one isolate from leftover refrigerated organic dog food, two isolates from urine and clay collected from an okapi (Okapia johnstoni) and antelope bongo (Tragelaphus eurycerus) enclosure in a zoological garden, one isolate from the soil in an eagle’s nest (Haliaeetus albicilla) band two isolates from two common vole (Microtus arvalis) livers from one cattle farm. All isolates were identified by biochemical tests, morphology and 16S rDNA sequencing. Also, retrospective screening for M. arupense occurrence within the collected isolates is presented. Keywords: 16S rDNA sequencing; non-tuberculous mycobacteria; ecology Non-tuberculous mycobacteria (NTM) are ubiq- According to the commonly used Runyon clas- uitous in the environment and are responsible for sification scheme, NTM are categorized by growth several diseases in humans and/or animals known rate and pigmentation.
    [Show full text]
  • Brief Communication
    BRIEF COMMUNICATION http://dx.doi.org/10.1590/S1678-9946201860006 Nontuberculous mycobacteria in milk from positive cows in the intradermal comparative cervical tuberculin test: implications for human tuberculosis infections Carmen Alicia Daza Bolaños1, Marília Masello Junqueira Franco1, Antonio Francisco Souza Filho2, Cássia Yumi Ikuta2, Edith Mariela Burbano-Rosero3, José Soares Ferreira Neto2, Marcos Bryan Heinemann2, Rodrigo Garcia Motta4, Carolina Lechinski de Paula1, Amanda Bonalume Cordeiro de Morais1, Simony Trevizan Guerra1, Ana Carolina Alves1, Fernando José Paganini Listoni1, Márcio Garcia Ribeiro1 ABSTRACT Although the tuberculin test represents the main in vivo diagnostic method used in the control and eradication of bovine tuberculosis, few studies have focused on the identification of mycobacteria in the milk from cows positive to the tuberculin test. The aim of this study was to identify Mycobacterium species in milk samples from cows positive to the comparative intradermal test. Milk samples from 142 cows positive to the comparative intradermal test carried out in 4,766 animals were aseptically collected, cultivated on Lowenstein-Jensen and Stonebrink media and incubated for up to 90 days. Colonies compatible with mycobacteria were stained by Ziehl-Neelsen to detect acid-fast bacilli, while to confirm the Mycobacterium genus, conventional PCR was performed. Fourteen mycobacterial strains were isolated from 12 cows (8.4%). The hsp65 gene sequencing identified M. engbaekii (n=5), M. arupense 1Universidade Estadual Paulista,
    [Show full text]
  • View of Results 1SGN the Overall Results of the Two Methods Are Summarized in M
    BMC Microbiology BioMed Central Research article Open Access Reliable identification of mycobacterial species by PCR-restriction enzyme analysis (PRA)-hsp65 in a reference laboratory and elaboration of a sequence-based extended algorithm of PRA-hsp65 patterns Erica Chimara1, Lucilaine Ferrazoli1, Suely Yoko Misuka Ueky1, Maria Conceição Martins1, Alan Mitchel Durham2, Robert D Arbeit3 and Sylvia Cardoso Leão*4 Address: 1Instituto Adolfo Lutz, São Paulo, Brazil, 2Instituto de Matemática e Estatística, Universidade de São Paulo, São Paulo, Brazil, 3Tufts University School of Medicine, Division of Geographic Medicine and Infectious Diseases, Boston, Massachusetts, USA and 4Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, São Paulo, Brazil Email: Erica Chimara - echimara@ial.sp.gov.br; Lucilaine Ferrazoli - lferrazoli@ial.sp.gov.br; Suely Yoko Misuka Ueky - satie@osite.com.br; Maria Conceição Martins - mcmartin@ial.sp.gov.br; Alan Mitchel Durham - alan@ime.usp.br; Robert D Arbeit - rda@ahkx.com; Sylvia Cardoso Leão* - sylvia.leao@unifesp.br * Corresponding author Published: 20 March 2008 Received: 17 October 2007 Accepted: 20 March 2008 BMC Microbiology 2008, 8:48 doi:10.1186/1471-2180-8-48 This article is available from: http://www.biomedcentral.com/1471-2180/8/48 © 2008 Chimara et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Identification of nontuberculous mycobacteria (NTM) based on phenotypic tests is time-consuming, labor-intensive, expensive and often provides erroneous or inconclusive results.
    [Show full text]
  • Frequency and Clinical Implications of the Isolation of Rare Nontuberculous Mycobacteria
    Kim et al. BMC Infectious Diseases (2015) 15:9 DOI 10.1186/s12879-014-0741-7 RESEARCH ARTICLE Open Access Frequency and clinical implications of the isolation of rare nontuberculous mycobacteria Junghyun Kim1, Moon-Woo Seong2, Eui-Chong Kim2, Sung Koo Han1 and Jae-Joon Yim1* Abstract Background: To date, more than 125 species of nontuberculous mycobacteria (NTM) have been identified. In this study, we investigated the frequency and clinical implication of the rarely isolated NTM from respiratory specimens. Methods: Patients with NTM isolated from their respiratory specimens between July 1, 2010 and June 31, 2012 were screened for inclusion. Rare NTM were defined as those NTM not falling within the group of eight NTM species commonly identified at our institution: Mycobacterium avium, M. intracellulare, M. abscessus, M. massiliense, M. fortuitum, M. kansasii, M. gordonae, and M. peregrinum. Clinical, radiographic and microbiological data from patients with rare NTM were reviewed and analyzed. Results: During the study period, 73 rare NTM were isolated from the respiratory specimens of 68 patients. Among these, M. conceptionense was the most common (nine patients, 12.3%). The median age of the 68 patients with rare NTM was 68 years, while 39 of the patients were male. Rare NTM were isolated only once in majority of patient (64 patients, 94.1%). Among the four patients from whom rare NTM were isolated two or more times, only two showed radiographic aggravation caused by rare NTM during the follow-up period. Conclusions: Most of the rarely identified NTM species were isolated from respiratory specimens only once per patient, without concomitant clinical aggravation.
    [Show full text]
  • Non-Tuberculous Mycobacteria: Epidemiological Pattern in a Reference Laboratory and Risk Factors Associated with Pulmonary Disease
    Epidemiol. Infect. (2017), 145, 515–522. © Cambridge University Press 2016 doi:10.1017/S0950268816002521 Non-tuberculous mycobacteria: epidemiological pattern in a reference laboratory and risk factors associated with pulmonary disease J. MENCARINI1,C.CRESCI2, M. T. SIMONETTI3,C.TRUPPA1, G. CAMICIOTTOLI2,4,M.L.FRILLI4,P.G.ROGASI5,S.VELOCI1, 2,4 3,6,7 1,5 M. PISTOLESI ,G.M.ROSSOLINI ,A.BARTOLONI AND F. BARTALESI5* 1 Infectious Diseases Unit, Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy 2 Pneumology Unit, Careggi Hospital, Florence, Italy 3 Tuscany Regional Reference Centre for Mycobacteria, Microbiology and Virology Unit, Careggi Hospital, Florence, Italy 4 Section of Respiratory Medicine, Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy 5 Unit of Infectious and Tropical Diseases, Careggi Hospital, Florence, Italy 6 Section of Microbiology, Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy 7 Department of Medical Biotechnologies, University of Siena, Siena, Italy Received 8 April 2016; Final revision 7 October 2016; Accepted 9 October 2016; first published online 2 November 2016 SUMMARY The diseases caused by non-tuberculous mycobacteria (NTM), in both AIDS and non-AIDS populations, are increasingly recognized worldwide. Although the American Thoracic Society published the guidelines for diagnosis of NTM pulmonary disease (NTM-PD), the diagnosis is still difficult. In the first part of the study, we collected data on NTM isolates in the Mycobacteriology Laboratory of Careggi Hospital (Florence, Italy) and analysed the epidemiological data of NTM isolates. Then, to analyse the risk factors associated to NTM-PD, we studied the presence of ATS/IDSA criteria for NTM-PD in patients who had at least one positive respiratory sample for NTM and were admitted to the Infectious Disease Unit and the Section of Respiratory Medicine.
    [Show full text]
  • The Impact of Chlorine and Chloramine on the Detection and Quantification of Legionella Pneumophila and Mycobacterium Spp
    The impact of chlorine and chloramine on the detection and quantification of Legionella pneumophila and Mycobacterium spp. Maura J. Donohue Ph.D. Office of Research and Development Center of Environmental Response and Emergency Response (CESER): Water Infrastructure Division (WID) Small Systems Webinar January 28, 2020 Disclaimer: The views expressed in this presentation are those of the author and do not necessarily reflect the views or policies of the U.S. Environmental Protection Agency. A Tale of Two Bacterium… Legionellaceae Mycobacteriaceae • Legionella (Genus) • Mycobacterium (Genus) • Gram negative bacteria • Nontuberculous Mycobacterium (NTM) (Gammaproteobacteria) • M. avium-intracellulare complex (MAC) • Flagella rod (2-20 µm) • Slow grower (3 to 10 days) • Gram positive bacteria • Majority of species will grow in free-living • Rod shape(1-10 µm) amoebae • Non-motile, spore-forming, aerobic • Aerobic, L-cysteine and iron salts are required • Rapid to Slow grower (1 week to 8 weeks) for in vitro growth, pH: 6.8 to 7, T: 25 to 43 °C • ~156 species • ~65 species • Some species capable of causing disease • Pathogenic or potentially pathogenic for human 3 NTM from Environmental Microorganism to Opportunistic Opponent Genus 156 Species Disease NTM =Nontuberculous Mycobacteria MAC = M. avium Complex Mycobacterium Mycobacterium duvalii Mycobacterium litorale Mycobacterium pulveris Clinically Relevant Species Mycobacterium abscessus Mycobacterium elephantis Mycobacterium llatzerense. Mycobacterium pyrenivorans, Mycobacterium africanum Mycobacterium europaeum Mycobacterium madagascariense Mycobacterium rhodesiae Mycobacterium agri Mycobacterium fallax Mycobacterium mageritense, Mycobacterium riyadhense Mycobacterium aichiense Mycobacterium farcinogenes Mycobacterium malmoense Mycobacterium rufum M. avium, M. intracellulare, Mycobacterium algericum Mycobacterium flavescens Mycobacterium mantenii Mycobacterium rutilum Mycobacterium alsense Mycobacterium florentinum. Mycobacterium marinum Mycobacterium salmoniphilum ( M. fortuitum, M.
    [Show full text]
  • Isolation of Non-Tuberculous Mycobacteria
    Kankya et al. BMC Public Health 2011, 11:320 http://www.biomedcentral.com/1471-2458/11/320 RESEARCHARTICLE Open Access Isolation of non-tuberculous mycobacteria from pastoral ecosystems of Uganda: Public Health significance Clovice Kankya1,2*, Adrian Muwonge2, Berit Djønne3, Musso Munyeme2,4, John Opuda-Asibo1, Eystein Skjerve2, James Oloya1, Vigdis Edvardsen3 and Tone B Johansen3 Abstract Background: The importance of non-tuberculous mycobacteria (NTM) infections in humans and animals in sub- Saharan Africa at the human-environment-livestock-wildlife interface has recently received increased attention. NTM are environmental opportunistic pathogens of humans and animals. Recent studies in pastoral ecosystems of Uganda detected NTM in humans with cervical lymphadenitis and cattle with lesions compatible with bovine tuberculosis. However, little is known about the source of these mycobacteria in Uganda. The aim of this study was to isolate and identify NTM in the environment of pastoral communities in Uganda, as well as assess the potential risk factors and the public health significance of NTM in these ecosystems. Method: A total of 310 samples (soil, water and faecal from cattle and pigs) were examined for mycobacteria. Isolates were identified by the INNO-Lipa test and by 16S rDNA sequencing. Additionally, a questionnaire survey involving 231 pastoralists was conducted during sample collection. Data were analysed using descriptive statistics followed by a multivariable logistic regression analysis. Results: Forty-eight isolates of NTM were detected; 25.3% of soil samples, 11.8% of water and 9.1% from animal faecal samples contained mycobacteria. Soils around water sources were the most contaminated with NTM (29.8%).
    [Show full text]
  • Mycobacterium Mephinesia
    www.nature.com/scientificreports OPEN “Mycobacterium mephinesia”, a Mycobacterium terrae complex species of clinical interest isolated Received: 28 November 2018 Accepted: 15 July 2019 in French Polynesia Published: xx xx xxxx Jamal Saad1, Michael Phelippeau2, May Khoder1, Marc Lévy3, Didier Musso4 & Michel Drancourt1 A 59-year-old tobacco smoker male with chronic bronchitis living in Taravao, French Polynesia, Pacifc, presented with a two-year growing nodule in the middle lobe of the right lung. A guided bronchoalveolar lavage inoculated onto Löwenstein-Jensen medium yielded colonies of a rapidly- growing non-chromogenic mycobacterium designed as isolate P7213. The isolate could not be identifed using routine matrix-assisted laser desorption ionization-time of fight-mass spectrometry and phenotypic and probe-hybridization techniques and yielded 100% and 97% sequence similarity with the respective 16S rRNA and rpoB gene sequences of Mycobacterium virginiense in the Mycobacterium terrae complex. Electron microscopy showed a 1.15 µm long and 0.38 µm large bacillus which was in vitro susceptible to rifampicin, rifabutin, ethambutol, isoniazid, doxycycline and kanamycin. Its 4,511,948- bp draft genome exhibited a 67.6% G + C content with 4,153 coding-protein genes and 87 predicted RNA genes. Genome sequence-derived DNA-DNA hybridization, OrthoANI and pangenome analysis confrmed isolate P7213 was representative of a new species in the M. terrae complex. We named this species “Mycobacterium mephinesia”. Te International Working Group on Mycobacterial Taxonomy delineated the Mycobacterium terrae complex in 19981. Te M. terrae complex initially consisted of two species Mycobacterium terrae and Mycobacterium non- chromogenicum1,2. M. nonchromogenicum had been described in 1965 by Tsukamura3, while M.
    [Show full text]
  • Utilization of Molecular and Conventional Methods for the Identification of Nontuberculous Mycobacteria Isolated from Different Water Sources
    [Downloaded free from http://www.ijmyco.org on Monday, June 4, 2018, IP: 200.41.178.226] Original Article Utilization of Molecular and Conventional Methods for the Identification of Nontuberculous Mycobacteria Isolated from Different Water Sources Claudia Andrea Tortone1, Martín José Zumárraga2, Andrea Karina Gioffré2, Delia Susana Oriani1 1Laboratory of Mycobacteria, Faculty of Veterinary Sciences, National University of La Pampa, General Pico, La Pampa, 2Biotechnology Institute, National Institute of Agricultural Technology (INTA), Hurlingham, Buenos Aires, Argentina Abstract Background: The environment is the nontuberculous mycobacteria (NTM) reservoir, opportunistic pathogens of great diversity and ubiquity, which is observed in the constant description of new species capable of causing infection. Since its introduction, molecular methods are essential for species identification. Most comparative studies between molecular and conventional methods, have used isolated strains from clinical samples. Methods: The aim of this study was to evaluate the usefulness of molecular methods, especially the hsp65‑PRA (PCR‑Restriction Enzyme Analysis), and biochemical tests in the identification of NTM recovered from water of different origins, using the sequencing of 16S rRNA and hsp65 genes as assessment methods of the previous ones. Species identification was performed for all 56 NTM isolates what were recovered from 32 (42.1%) positive water samples, using conventional phenotypic methods, hsp65‑PRA, partial sequencing of 16S rRNA and sequencing
    [Show full text]
  • Characterization of Photochromogenic Mycobacterium Spp. from Chesapeake Bay Striped Bass Morone Saxatilis D
    Old Dominion University ODU Digital Commons Biological Sciences Faculty Publications Biological Sciences 2011 Characterization of Photochromogenic Mycobacterium spp. from Chesapeake Bay Striped Bass Morone Saxatilis D. T. Gauthier Old Dominion University, dgauthie@odu.edu A. M. Helenthal M. W. Rhodes W. K. Vogelbein H. I. Kator Follow this and additional works at: https://digitalcommons.odu.edu/biology_fac_pubs Part of the Aquaculture and Fisheries Commons, and the Bacteriology Commons Repository Citation Gauthier, D. T.; Helenthal, A. M.; Rhodes, M. W.; Vogelbein, W. K.; and Kator, H. I., "Characterization of Photochromogenic Mycobacterium spp. from Chesapeake Bay Striped Bass Morone Saxatilis" (2011). Biological Sciences Faculty Publications. 170. https://digitalcommons.odu.edu/biology_fac_pubs/170 Original Publication Citation Gauthier, D. T., Helenthal, A. M., Rhodes, M. W., Vogelbein, W. K., & Kator, H. I. (2011). Characterization of photochromogenic Mycobacterium spp. from Chesapeake Bay striped bass Morone saxatilis. Diseases of Aquatic Organisms, 95(2), 113-124. doi:10.3354/ dao02350 This Article is brought to you for free and open access by the Biological Sciences at ODU Digital Commons. It has been accepted for inclusion in Biological Sciences Faculty Publications by an authorized administrator of ODU Digital Commons. For more information, please contact digitalcommons@odu.edu. Vol. 95: 113–124, 2011 DISEASES OF AQUATIC ORGANISMS Published June 16 doi: 10.3354/dao02350 Dis Aquat Org Characterization of photochromogenic Mycobacterium spp. from Chesapeake Bay striped bass Morone saxatilis D. T. Gauthier1,*, A. M. Helenthal1, M. W. Rhodes2, W. K. Vogelbein2, H. I. Kator2 1Department of Biological Sciences, Old Dominion University, Norfolk, Virginia 23529, USA 2Virginia Institute of Marine Science, The College of William and Mary, Department of Environmental and Aquatic Animal Health, Gloucester Point, Virginia 23062, USA ABSTRACT: A large diversity of Mycobacterium spp.
    [Show full text]
  • A Novel Non-Tuberculous Mycobacterium Species Revealed by Multiple Gene Sequence Characterization
    Mycobacterium malmesburyense sp. nov: A novel non-tuberculous Mycobacterium species revealed by multiple gene sequence characterization *Nomakorinte Gcebe1, 2, Victor Rutten 2, 3, Nicolaas Gey van Pittius 4 , Brendon Naicker 5, Anita Michel2 1Tuberculosis Laboratory, Agricultural Research Council - Onderstepoort Veterinary Institute, Onderstepoort, South Africa; 2Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa; 3Division of Immunology, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands; 4Centre of Excellence for Biomedical Tuberculosis Research, Division of Molecular Biology and Human Genetics, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Stellenbosch University, Tygerberg, South Africa; 5Polymers and Composites, Materials Science and Manufacturing, Council for Scientific and Industrial Research, Brummeria, South Africa *Author for correspondence- Email: gceben@arc.agric.za , Tel: +2712 5299138 The Genbank accession numbers for the 16S rRNA, hsp65, rpoB and sodA genes of WCM 7299T are KJ873241; KJ873243; KJ873245 and KJ873247 respectively. 1 ABSTRACT Non-tuberculous mycobacteria (NTM) are ubiquitous in the environment and an increasing number of NTM species have been isolated and characterized from both humans and animals, highlighting the zoonotic potential of these bacteria. Host exposure to NTM may impact on cross-reactive immune responsiveness which may affect diagnosis of bovine tuberculosis and may also play a role in the variability of the efficacy of Mycobacterium bovis BCG vaccination against tuberculosis. In this study we characterized 10 NTM isolates originating from water, soil, nasal swabs of cattle and African buffalo as well as bovine tissue samples. These isolates were previously identified during an NTM survey and were all found, using 16S rDNA sequence analysis to be closely-related to Mycobacterium moriokaense.
    [Show full text]
  • Extra-Pulmonary Nontuberculous Mycobacterial Infections: 16 Year Retrospective Analysis at an Academic Institution in Cincinnati, Ohio
    Extra-pulmonary Nontuberculous Mycobacterial Infections: 16 year retrospective analysis at an academic institution in Cincinnati, Ohio. A thesis submitted to the Graduate School of the University of Cincinnati in partial fulfillment of the requirements for the degree of Master of Science in Clinical & Translational Research In the Department of Environmental Health Division of Epidemiology of the College of Medicine July, 2017 by Kiran Afshan MBBS, University of Karachi, Pakistan, August, 2006 Committee Chair: Erin Haynes, DrPH Abstract Title: Extra-pulmonary Nontuberculous Mycobacterial Infections: 16 year retrospective analysis at an academic institution in Cincinnati, Ohio. Authors: Afshan K, Smulian AG, Jandarov RA, Haglund L. Background: Nontuberculous mycobacterial infections (NTM), once considered a rare cause of human disease, are now increasingly recognized in clinical practice. We have sought to identify clinical and demographic characteristics of the extra-pulmonary NTM infections presenting at our institution during 2000-2015. Methods: Records of patient with culture proven extra-pulmonary NTM infections were reviewed. Demographic information, clinical and microbiologic characteristics and treatment outcomes were captured. Results: 58 cases of extra-pulmonary NTM infections identified were classified into cutaneous 9 (15.52%), soft tissue 38 (65.52%), osteo-articular 9 (15.52%) and disseminated infections 2 (3.45%). 52% of the cases were male. Median age at diagnosis was 52years. All cases were diagnosed based on culture positivity.
    [Show full text]