Antibodies for Cancer Immunology Immunotherapy Research

Immune Checkpoint Blockade STING Pathway Investigate co-stimulatory and co-inhibitory Interrogate the STING Pathway by Western blot, IHC, or Flow. molecules with high-quality, multi-application STING (Stimulator of Interferon Genes) is a detector of intracel- validated antibodies. Co-inhibitory and co-stimulatory mole- lular viral molecules and double stranded DNA. Activation of cules play a critical role in T cell activation and STING triggers phosphorylation of downstream NAK/TBK1 and tumor cell recognition and killing. Along with MHC/TCR IRF3 to activate immunity and a type I interferon response. engagement, co-signaling molecules direct the outcome of T The ability of STING agonists to activate a potent anti-tumor cell activation. In the context of cancer, tumor cells exploit the immune response has driven increased interest in the path- upregulation of co-inhibitory molecules to way for cancer immunotherapy. promote their own survival and avoid immune recognition.

T cell APC dsDNA OX40 OX40L CD27 CD27L Costimulatory CD137 CD137L cGAS Type I IFNs CD28 CD80/CD86 +ATP +GTP TCR MHC cGAMP p65 p60 CTLA-4 CD80/CD86 NF-kB PD1 PD-L1/PD-L2 STING CoinhibitoryCD80 PD-L1 P P IRF3 TBK1 IRF3 IRF3 TIM3 GAL9

VISTA/PD-1H Antibody PD-L1 Antibody IRF3 Antibody (2G3) STING Antibody NBP1-88967 MAB1561 NBP1-47812 NBP2-24683

IHC: VISTA staining of IHC: PD-L1 staining IHC: IRF3 staining of IHC: STING staining of human tonsil. of human colon cancer. adenocarcinoma of colon tissue. human breast tumor. (2 Publications) (12 Publications)

LAG-3 Antibody PD-1 Antibody NAK/TBK1 Antibody RelA/NFkB p65 Antibody NBP1-97657 AF1086 NB100-56705 NB100-2176

Flow: LAG-3 FITC staining IHC: PD-1 staining of IHC: TBK1 on human IHC: RelA staining of human in resting and PHA activated human lymph node. testis. DLBCL showing nuclear expression lymphocytes. in the tumor cells. (9 Publications) (10 Publications) (20 Publications) (19 Publications)

Learn more | novusbio.com Purinergic Signaling Myeloid Cell Biology and The Quantify ATP levels and Probe Purinergic Signaling. Tumor Microenvironment Similar to inhibitory members of the B7 family, adenosine Understand the tumor microenvironment and signaling dampens anti-tumor immunity. The purine myeloid cell biology with Novus antibodies. nucleotide, ATP, is converted by extracellular receptors Suppressive myeloid cells in the tumor microenvironment to adenosine. This molecule signals through G-protein Mouse Human inhibit anti-tumor immunity. By secreting suppressive and + coupled+ receptors, including the A2A receptor, to mediate CD11b Cd11b angiogenic molecules, tumor-associated macrophages and Gr-Ly1/Ly-6G- immunosuppresiveHLA-Dr- responses. It has been demonstrated myeloid-derived suppressor cells promote tumor growth Ly-6C+ thatCD14 adenosine+ receptor blockade enhances anti-tumor - and survival. Understanding myeloid cell biology is key to immunity.Lin Because of its potential to regulate immunity, - developing improved immunotherapies. adenosineCD66b signaling is considered next generation immune CD15- checkpoint blockade.

Mouse Human CD11b+ Cd11b+ + - - Pannexin-1 MonocyticGranulocytic Gr-1/Ly-6G HLA Dr ATP - - Ly-6C CD14 Mouse monocytic: Mouse granulocytic: - + high lo Lin P2X CD11b+ Gr-1/Ly-6Glo Ly-6Chigh CD11b Gr-1/Ly-6G Ly-6C CD66b+ 2 Human monocytic: Human granulocytic: CD15+ CD39 P2Y - + + – Lin–CD11b+ CD14– CD15+ ADP Lin CD11b CD14 CD15 + + – CD73 AR CD33+ CD66b– HLA-DR– CD33 CD66b HLA-DR ATP

NT 2 3 AMP AMP Adenosine Adenosine Stimulatory vs. Suppressive Myeloid Cells

A1 receptor A2A receptor A2B receptor A3 receptor Stimulatory Suppressive IL-12hi IL-10lo IL-12lo IL-10hi Gsor Gq Gs G or G G or G i q i o ROS TGF-B RNI Arginase

Adenosine A2a CD39 Antibody CD11b Antibody HLA-DR Antibody Receptor Antibody NBP1-90071 NB110-40766 NB100-77855 NB300-597

ICC/IF: Adenosine A2a ICC/IF: CD39 antibody Flow: Detection of CD11 Flow: HLA-DR expression receptor antibody staining in staining in human aortic b/c in fixed Hela cells. in BDCM cells. the cytoplasm of U251 cells. valve endothelial cells. (1 Publication) (4 Publications) (16 Publications) (26 Publications)

CD73 Antibody HIF-1 alpha Antibody CD68 Antibody iNOS Antibody NBP1-85740 NB100-105 NB100-683 NB300-605

IHC: CD73 staining of WB; HIF-1 alpha analysis IHC staining of CD68 in WB: iNOS staining in human endometrium. of COS-7 nuclear extracts. human spleen. stimulated astrocytes. (5 Publications) (666 Publications) (33 Publications) (20 Publications)

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