Evaluating Recovery Potential of the Northern White Rhinoceros from Cryopreserved Somatic Cells
Downloaded from genome.cshlp.org on September 26, 2021 - Published by Cold Spring Harbor Laboratory Press Research Evaluating recovery potential of the northern white rhinoceros from cryopreserved somatic cells Tate Tunstall,1 Richard Kock,2 Jiri Vahala,3 Mark Diekhans,4 Ian Fiddes,4 Joel Armstrong,4 Benedict Paten,4 Oliver A. Ryder,1,5 and Cynthia C. Steiner1,5 1San Diego Zoo Institute for Conservation Research, Escondido, California 92027, USA; 2Royal Veterinary College, University of London, London NW1 0TU, United Kingdom; 3Dvur Krlov Zoo, Dvr Krlov nad Labem 544 01, Czech Republic; 4Jack Baskin School of Engineering, University California Santa Cruz, Santa Cruz, California 95064, USA The critically endangered northern white rhinoceros is believed to be extinct in the wild, with the recent death of the last male leaving only two remaining individuals in captivity. Its extinction would appear inevitable, but the development of advanced cell and reproductive technologies such as cloning by nuclear transfer and the artificial production of gametes via stem cells differentiation offer a second chance for its survival. In this work, we analyzed genome-wide levels of genetic diversity, inbreeding, population history, and demography of the white rhinoceros sequenced from cryopreserved somatic cells, with the goal of informing how genetically valuable individuals could be used in future efforts toward the genetic res- cue of the northern white rhinoceros. We present the first sequenced genomes of the northern white rhinoceros, which show relatively high levels of heterozygosity and an average genetic divergence of 0.1% compared with the southern sub- species. The two white rhinoceros subspecies appear to be closely related, with low genetic admixture and a divergent time <80,000 yr ago.
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