micromachines Review Electrophysiology Read-Out Tools for Brain-on-Chip Biotechnology Csaba Forro 1,2,†, Davide Caron 3,† , Gian Nicola Angotzi 4,†, Vincenzo Gallo 3, Luca Berdondini 4 , Francesca Santoro 1 , Gemma Palazzolo 3,* and Gabriella Panuccio 3,* 1 Tissue Electronics, Fondazione Istituto Italiano di Tecnologia, Largo Barsanti e Matteucci, 53-80125 Naples, Italy;
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[email protected] (F.S.) 2 Department of Chemistry, Stanford University, Stanford, CA 94305, USA 3 Enhanced Regenerative Medicine, Fondazione Istituto Italiano di Tecnologia, Via Morego, 30-16163 Genova, Italy;
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[email protected] (V.G.) 4 Microtechnology for Neuroelectronics, Fondazione Istituto Italiano di Tecnologia, Via Morego, 30-16163 Genova, Italy;
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[email protected] (L.B.) * Correspondence:
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[email protected] (G.P.); Tel.: +39-010-2896-884 (G.P.); +39-010-2896-493 (G.P.) † These authors contributed equally to this paper. Abstract: Brain-on-Chip (BoC) biotechnology is emerging as a promising tool for biomedical and pharmaceutical research applied to the neurosciences. At the convergence between lab-on-chip and cell biology, BoC couples in vitro three-dimensional brain-like systems to an engineered microfluidics platform designed to provide an in vivo-like extrinsic microenvironment with the aim of replicating tissue- or organ-level physiological functions. BoC therefore offers the advantage of an in vitro repro- duction of brain structures that is more faithful to the native correlate than what is obtained with conventional cell culture techniques.