Drosophila a Virus Is an Unusual RNA Virus with a T53 Icosahedral Core and Permuted RNA- Dependent RNA Polymerase

Journal of General Virology (2009), 90, 2191–2200 DOI 10.1099/vir.0.012104-0 Drosophila A virus is an unusual RNA virus with a T53 icosahedral core and permuted RNA- dependent RNA polymerase Rebecca L. Ambrose,1 Gabriel C. Lander,2 Walid S. Maaty,3 Brian Bothner,3 John E. Johnson2 and Karyn N. Johnson1 Correspondence 1School of Biological Sciences, The University of Queensland, Brisbane, Australia Karyn N. Johnson 2Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA, USA [email protected] 3Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT, USA The vinegar fly, Drosophila melanogaster, is a popular model for the study of invertebrate antiviral immune responses. Several picorna-like viruses are commonly found in both wild and laboratory populations of D. melanogaster. The best-studied and most pathogenic of these is the dicistrovirus Drosophila C virus. Among the uncharacterized small RNA viruses of D. melanogaster, Drosophila A virus (DAV) is the least pathogenic. Historically, DAV has been labelled as a picorna-like virus based on its particle size and the content of its RNA genome. Here, we describe the characterization of both the genome and the virion structure of DAV. Unexpectedly, the DAV genome was shown to encode a circular permutation in the palm-domain motifs of the RNA-dependent RNA polymerase. This arrangement has only been described previously for a subset of viruses from the double-stranded RNA virus family Birnaviridae and the T54 single-stranded RNA virus family Tetraviridae. The 8 A˚ (0.8 nm) DAV virion structure computed from cryo-electron microscopy and image reconstruction indicates that the virus structural protein forms two discrete domains within the capsid. The inner domain is formed from a clear T53 lattice with similarity to the b-sandwich domain of tomato bushy stunt virus, whilst the outer domain is not ordered icosahedrally, but forms a cage-like structure that surrounds the core Received 29 March 2009 domain. Taken together, this indicates that DAV is highly divergent from previously described Accepted 27 May 2009 viruses. INTRODUCTION of the range and specificity of Drosophila responses to virus infection, it would be valuable to use viruses that naturally As obligate intracellular pathogens, viruses have complex infect Drosophila with a range of pathogenicities. interactions with their hosts. Comparatively little is known about the specific responses to virus infection in insects. During the 1970s, 11 viruses were described from Recently, insect responses that are specific for virus Drosophila (Brun & Plus, 1980; Gateff et al., 1980; Jousset infection have been investigated by using the genetically & Plus, 1975; Plus, 1978). Three of these, which were tractable Drosophila melanogaster (vinegar fly) model and isolated from D. melanogaster, were described as picorna- the most pathogenic of its viruses, the dicistrovirus like viruses based on morphological and biophysical Drosophila C virus (DCV) (Cherry & Perrimon, 2004; characteristics. The three viruses, DCV, Drosophila A virus Cherry et al., 2005; Deddouche et al., 2008; Ding & (DAV) and Drosophila P virus, were shown to be different Voinnet, 2007; Dostert et al., 2005; Galiana-Arnoux et al., viruses based on serology and sizes of the capsid proteins 2006; Hedges & Johnson, 2008; Hedges et al., 2008; (Jousset et al., 1972; Plus et al., 1976). These viruses are Roxstrom-Lindquist et al., 2004; Sabatier et al., 2003; natural pathogens of D. melanogaster, with about 40 % of Teixeira et al., 2008; van Rij et al., 2006) and the birnavirus wild populations being infected with one or more of these Drosophila X virus (Zambon et al., 2005). In future studies viruses (Plus et al., 1975). The three viruses have been shown to vary in their pathogenicity, with DCV being the The GenBank/EMBL/DDBJ accession number for the DAV genome most pathogenic and DAV the least. It is important to have sequence reported in this paper is FJ150422. an understanding of molecular characteristics of viruses to Two supplementary figures, showing alignments of the RdRP and the facilitate these studies (Huszar & Imler, 2008). Whilst DCV deduced capsid protein sequences of DAV with those of other RNA has been well-characterized and belongs to the genus viruses, are available with the online version of this paper. Cripavirus of the family Dicistroviridae (Christian et al., 012104 G 2009 SGM Printed in Great Britain 2191 R. L. Ambrose and others 2005; Johnson & Christian, 1998), no detailed character- and stored at 220 uC until use. DAVHD virions were also isolated ization of the DAV genome or virion structure has been from infected DL2 cells by using a similar method after cells were reported. lysed by repetitive freeze–thawing at 220 uC. There are many small RNA viruses of insects that have Transmission electron microscopy (TEM) of purified virus. non-enveloped, 25–40 nm particles that encapsidate pos- Purified DAVHD was analysed by TEM as described previously itive-sense RNA genomes (Christian & Scotti, 1998; (Johnson & Ball, 2003). Fauquet et al., 2005). Of these, the majority with sequenced Nuclease treatment. For nuclease treatment, 1.2 mg DAVHD genomes are included in four taxonomic groups: the double-stranded (ds) RNA, prepared in vitro as described previously families Nodaviridae, Dicistroviridae and Tetraviridae and (Hedges & Johnson, 2008), and Flock House virus (FHV) RNA the floating genus Iflavirus (Fauquet et al., 2005). However, (Johnson et al., 2001) was incubated at 37 uC with 10 U RNase ONE in recent years, a number of viruses from insects have been (Promega) for 45 min. Samples were then diluted in RNA loading characterized that do not fit clearly into one of these buffer (Ambion), heated to 65 uC and separated on a non-denaturing 1 % agarose gel; products were visualized by staining with ethidium groups (Habayeb et al., 2006; Hartley et al., 2005; van der bromide. Wilk et al., 1997). Furthermore, within the family Tetraviridae, there are viruses with diverse genome cDNA synthesis, cloning and sequence determination. RNA was organizations that are classified together because of the extracted from sucrose gradient-purified DAVHD virions by using T54 symmetry of their capsid particles (Agrawal & TriReagent (Sigma-Aldrich) as per the manufacturer’s instructions, with Johnson, 1992; Gorbalenya et al., 2002; Gordon et al., the addition of 20 mg glycogen to increase RNA recovery. First- and 1995, 1999; Hanzlik et al., 1995; Pringle et al., 2001, 2003). second-strand cDNA was synthesized by using a SuperScript double- stranded cDNA synthesis kit (Invitrogen) with random hexamers as As more invertebrate RNA viruses are characterized fully, it recommended by the manufacturer. The cDNA products were ligated is becoming clear that there is a previously unrecognized into the EcoRV site of phosphatase-treated pBluescript SK+ (Stratagene) diversity of genome organization and expression strategies and sequenced. From these sequences, DAV-specific primers were within this group of viruses. These findings give us insight designed and used for further cDNA synthesis using SuperScript reverse both into virus evolution and into other biological transcriptase III (Invitrogen) as per the manufacturer’s instructions. processes (Koonin et al., 2008). 59 RACE (random amplification of cDNA ends) was performed as indicated by the manufacturer (Invitrogen) to obtain sequence at the 59 Here, we describe the molecular and structural character- end of the virus genome. A poly(A) tail was added to the 39 end of ization of DAV. The 30 nm particles encapsidate a purified DAVHD RNA by using poly(A) polymerase (Ambion), and the 39 positive-sense RNA that encodes two large open reading RACE system (Invitrogen) was used as per the manufacturer’s instructions to clone the 39 end of the DAV RNA. Nucleotide sequences frames (ORFs). The first of these has significant sequence were assembled and analysed by using DNASTAR software. similarities to the RNA-dependent RNA polymerases (RdRPs) of the tetraviruses and birnaviruses that have Cells. Drosophila DL2 cells (Schneider, 1972) were maintained at permuted RdRPs, whilst the second ORF encodes the 26 uC in Schneider’s medium (Invitrogen) supplemented with 10 % capsid protein. Analysis of the structure of DAV virions heat-inactivated fetal bovine serum and antibiotics. DL2 cells were 6 shows a unique capsid arrangement. seeded at 7610 cells per well into six-well tissue-culture plates and left to adhere at 26 uC overnight. Cells were infected with purified DAVHD virions in 500 ml additive-free Schneider’s medium or mock- infected and supplemented with 1.5 ml Schneider’s complete medium METHODS following incubation at 26 uC for 2 h. Infected cells were harvested at 0, 1, 2, 3 and 5 days post-infection (p.i.), washed in PBS and lysed in Propagation of DAV in D. melanogaster. A stock of Champetie`res either 1 ml TriReagent or 50 ml SDS-PAGE loading buffer. flies was obtained from the Drosophila Genetic Resource Centre of the Kyoto Institute of Technology, Japan (stock no. 103403), and a virus- SDS-PAGE and Western blot analysis. Virus and cell lysate free population was established essentially as described by Brun & Plus samples were heated at 99 uCin16 sample buffer for 5 min before (1980). The Australian isolate DAV was kindly provided by Dr Peter HD electrophoresis through SDS–10 % polyacrylamide gels at 170 V. Christian (Christian, 1992). DAV was diluted in sterile insect saline HD Alternatively, samples were loaded with sample buffer and a reducing (0.6 % NaCl, 0.04 % KCl, 0.024 % CaCl2 and 0.02 % NaHCO3)and agent on a NuPAGE 4–12 % Bis–Tris gradient gel in MES running injected into the haemocoel of virus-free D. melanogaster. Injected flies buffer (Invitrogen) and electrophoresed at 200 V. Proteins were were maintained on standard Drosophila medium (Sigma) for 10 days visualized by staining with Coomassie brilliant blue. at 25 uC and then frozen at 220 uC until purification.

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