Article Molecular Mechanism of LEDGF/p75 Dimerization Graphical Abstract Authors Vanda Lux, Tine Brouns, Katerina Cerma ´ kova´ , ..., Frauke Christ, Zeger Debyser, Va´ clav Veverka Correspondence
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[email protected] (V.V.) In Brief Dimerization is an important process regulating eukaryotic transcription. Lux et al. use a combination of biophysical and biochemical techniques to investigate the dimerization mechanism of LEDGF/p75, the H3K36 methylation reader, and its effect on molecular interactions with other proteins. Highlights d Full-length LEDGF/p75 forms dimers with a low micromolar KD d LEDGF/p75 residues 345–467 is the minimal stable dimerization domain d LEDGF dimer is stabilized by domain swapping and additional electrostatic stapling d LEDGF dimerization does not impair binding of interaction partners Lux et al., 2020, Structure 28, 1288–1299 December 1, 2020 ª 2020 Elsevier Ltd. https://doi.org/10.1016/j.str.2020.08.012 ll ll Article Molecular Mechanism of LEDGF/p75 Dimerization Vanda Lux,1,7 Tine Brouns,2,7 Katerina Cerma´ kova´ ,1,3 Pavel Srb,1 Milan Fa´ bry,4 Marcela Ma´ dlı´kova´ ,1 Magdalena Horejsı´,4 Zdenek Kukacka, 5 Petr Nova´ k,5 Michael Kugler,1 Jirı´ Brynda,1,4 Jan DeRijck,2 Frauke Christ,2 Zeger Debyser,2,* and Va´ clav Veverka1,6,8,* 1Structural Biology, Institute of Organic Chemistry and Biochemistry of the CAS, Prague 16000, Czech Republic 2Molecular Virology and Gene Therapy, KU Leuven, Molecular Virology and Gene Therapy, Leuven, 3000 Flanders, Belgium 3Department