DOCSLIB.ORG

Genome-Resolved Metagenomic Analyses Reveal the Presence of a Bacterial Endosymbiont in an Avian Nasal Mite (Rhinonyssidae; Mesostigmata)

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text

Load more

bioRxiv preprint doi: https://doi.org/10.1101/2021.07.12.452008; this version posted July 12, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Genome-resolved metagenomic analyses reveal the presence of a bacterial endosymbiont in an avian nasal mite (Rhinonyssidae; Mesostigmata) Carolina Osuna-Mascaró 1,, Jorge Doña 2,3, Kevin P. Johnson 2, Manuel de Ro as !," 1 De#ar$%en$ of Biology, )niversi$( of *evada, Reno+ [email protected] 2 -llinois *atural His$ory /urve(, Prairie Research -ns$i$u$e, )niversi$( o& -llinois at )r0ana-Cha%#aign, Cham#aign, )/1+ [email protected] 2 -llinois *atural His$ory /urve(, Prairie Research -ns$i$u$e, )niversi$( o& -llinois at )r0ana-Cha%#aign, Cham#aign, )/1+ 2# ohnso,illinois.edu 3De#ar$amen$o de Biología Ani%al, )niversidad de 4ranada, 4ranada, /#ain 4 De#ar$%en$ of Micro0iology and Parasi$olog(, 5acul$y of Phar%ac(, )niversidad de /evilla, /evilla, /#ain+ dero as,us.es Abstract: Rhinon(ssidae (Mesos$igmata) is a &amil( o& nasal mites onl( &ound in 0irds. 1ll species are hema$o#hagous endo#arasites, 8hich ma( damage $he nasal cavities o& 0irds, and also could 0e #o$ential reservoirs or vec$ors o& o$her infec$ions. Ho8ever, $he role o& me%0ers o& Rhinon(ssidae as disease vec$ors in 8ild 0ird #o#ulations remains uninves$igated, 8ith s$udies o& $he micro0iomes o& Rhinon(ssidae 0eing almos$ non-exis$ent. -n $he nasal mite 6Tinaminyssus melloi7 fro% roc2 doves 6Columba livia), a #revious s$ud( &ound evidence o& a highl( abundant #u$ativel( endosy%0io$ic 0ac$eria fro% Class 1lphapro$eo0ac$eria. Here, 8e e9#anded $he sam#le size o& $his species, incor#orated contamination controls, and increased se;uencing de#$h in sho$gun se;uencing and genome-resolved me$agenomic anal(ses. Our goal 8as $o increase $he information regarding $his mite species 8i$h its #u$ative endosy%0iont. Our results su##or$ $he endosy%0io$ic nature o& $his 0ac$erial $axon, 8hich is $he <rs$ described &or 0ird's nasal mites $o date, and im#rove $he overall unders$anding o& $he micro0io$a inhabiting these mites. *Corres#onding au$hors> COM> cosuna,unr.edu+ MR, e%ail> dero as,us.es Ke(8ords> Rhinonyssidae+ endos(%0ion$+ %e$ageno%ic; Brucella bioRxiv preprint doi: https://doi.org/10.1101/2021.07.12.452008; this version posted July 12, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 1. Introd!ction Mites are one of the mos$ diverse grou#s of eu2ar(o$es on ear$h [1]. Mites are u0i;uitous, occu#(ing aquatic, terres$rial, and ar0oreal habitats [2-8]. On some occasions, mites have an intimate association (sy%0iosis) with a different organism, which, in the mos$ e9$re%e scenario (permanent sy%0ionts), re#resents the habitat in which the( undergo their entire life-c(cle [9]. For exam#le, a single species of insec$, mammal, or bird can o&$en hos$ several mite species and have different t(#es of interac$ions with the% [9, 10-12]. The nature of these interac$ions can range from mu$ualism (e.g., mites inhabiting and cleaning birds' feathers; [13]) to parasitism (e.g., mites inhabiting the nasal passages and lungs of seals causing illness $o the%+ [14, 15]). -n some cases, mites can affec$ the hos$'s heal$h and be responsible for transmit$ing zoono$ic diseases [9]. For exam#le, the itch mite Sarcoptes scabiei has caused the po#ulation decline of different mammal species b( transmit$ing scabies [16-19]. In ano$her case, Varroa destructor, a parasitic mite of the Asian hone(0ee (Apis cerana) has caused the decline of the Huro#ean hone(0ee b( transmit$ing a virus [20]. In some cases, disease transmission ma( be mediated b( endosy%0io$ic pa$hogens that inhabit the mites. One exa%#le of this mechanism is Leptotrombidium scutellare, a mite tha$ parasitizes mice and carries the endosy%0iont 0ac$eria Orientia tsutsugamushi, responsible for the scru0 t(#hus disease [21]. Endosy%0ionts as causal agents of diseases have also been widel( re#or$ed in tic2s [22-24]. Ho8ever, exa%#les of endosy%0ionts in mites are s$ill rare. Rhinon(ssidae (Mesos$igmata) is a famil( of nasal mites with more than 500 species described world8ide [25-28]. The( have been descri0ed from birds [29]. Almos$ all species of 0irds are inhabited b( nasal mites, which usuall( live in the nasal cavit( on vascularized e#i$helial tissue [9]. All rhinon(ssid species are hema$o#hagous endo#arasites [30]. /#ecificall(, rhinon(ssid mites damage the nasal cavities of birds, which, in rare cases, ma( lead to the death of the hos$s (Rhinon(ssidosis aviu% disease7 [28]. Moreover, it has been bioRxiv preprint doi: https://doi.org/10.1101/2021.07.12.452008; this version posted July 12, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. sugges$ed that these mites could be po$ential reservoirs or vec$ors of o$her infec$ions (such as Ies$ Nile fever, Q fever, avian influenza, and L(me diseases), as demons$rated in mites of the &amil( Derman(ssidae [31]. Ho8ever, the role of species of Rhinon(ssidae as disease vec$ors in wild bird po#ulations is ye$ to be unders$ood. In addition, s$udies on rhinon(ssid micro0iomes more generall( are almos$ non-exis$ent. -n a preliminar( previous s$ud(, the micro0iome of t8o different s#ecies of rhinon(ssid mites (Tinaminyssus melloi and Ptilonyssus chlori) from t8o different avian hos$ species 6Columba livia and Chloris chloris, respec$ivel(7 was charac$erized [32]. The results of that s$ud( sugges$ed that the nasal mite Tinaminyssus melloi har0ored a po$ential endosy%0iont 1lphapro$eo0ac$eria (Famil(: Bar$onellaceae) in a high abundance. Ho8ever, the lo8 se;uencing coverage, small sam#le size, and lac2 of control sam#les did no$ allo8 de<nitive conclusions to be made. In par$icular, onl( a par$ial (~26%) asse%0l( of the bac$erial genome 8as achieved, and it could no$ be ruled ou$ co%#le$el( if this bac$erial taxon came from the 0ird hos$ ins$ead of the mite. Here, we focused on e9#anding kno8ledge of the micro0iome of this mite species 6Tinaminyssus melloi), e%#hasizing increasing the information of its association wi$h this #u$ative endosy%0iont. In the current s$ud(, we e9#anded the sam#le size, used contamination controls, and increased se;uencing de#$h. In par$icular, we collec$ed t8o mite #ools (five mite individuals per pool) plus a control bird saliva sam#le from t8o different individual Roc2 doves (Columba livia). Then, we conduc$ed sho$gun and genome-resolved me$agenomic anal(ses to charac$erize the miteOs micro0iome along with evaluating the genome pro#er$ies of this bac$erial taxon, which ma( be informative regarding its endosy%0io$ic s$atus. 2. Materials and Methods 1 to$al of ten nasal mites were collec$ed from t8o different freshl( dead Columba livia individuals (i.e., 5 mites per individual hos$). The nasal cavities of the birds were dissec$ed bioRxiv preprint doi: https://doi.org/10.1101/2021.07.12.452008; this version posted July 12, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. under a s$ereomicrosco#e, and the mites were taxonomicall( identified. A saliva sam#le was also collec$ed from each individual. Mite and saliva sam#les were preserved at -20°C in tu0es 8i$h 100% e$hanol. 'e&ore DN1 isolation, sam#les were washed three times with e$hanol to remove #ossible e9$ernal contaminants follo8ing [13] and [33]. To$al genomic DN1 was isolated fro% all sam#les using the Quic2-DN1 MicroPre# kit (Z(mo), specificall( designed to isolate DN1 from small sam#les. A sam#le that did no$ contain tissue was included and treated as a regular sam#le to chec2 for cross-contamination during the DN1 isolation procedure. To$al DN1 was quantified using the Qu0it High Sensitivit( dsDN1 Assa( (Thermo Fisher Scientific). Libraries were pre#ared using the Ne9$era DN1 Flex Librar( Pre# kit (Illumina), s$ric$l( follo8ing the manu&ac$urer's ins$ruc$ions. BrieK(, DN1 was enzymaticall( cu$, and ada#$ers were added in a single s$e#. The ligated DN1 was am#lified, and oligonucleo$ide indices were added to bo$h ends of the frag%ents for pos$-se;uencing de%ultiplexing. The cons$ruc$ed libraries were quantified with the Qu0it dsDN1 HT Assa( kit (Ehermo Scientific), and qualit( chec2ed on an Agilent 2100 Bioanal(zer (Agilent Technologies). According to the Ju0it results, the libraries were pooled in e;uimolar amounts, and this pool was se;uenced on a NovaSe; PH150 single lane frac$ion (Illumina), aiming for a to$al ou$#u$ of 30 gigabases. Ehe DN1 isolation, am#lification, librar( pre#ara$ion, and whole-genome se;uencing were carried ou$ in AllGene$ics & Biolog( SL (w88.allgene$ics.eu). 5or the genome-resolved me$agenomic anal(ses, we trimmed the ra8 reads using fas$# [34].
Recommended publications
  • Ticks and Mites from a Wild Bird Survey Performed by the Wild Animal Medical Center of Rakuno Gakuen University in Japan

    Ticks and Mites from a Wild Bird Survey Performed by the Wild Animal Medical Center of Rakuno Gakuen University in Japan

    J. Acarol. Soc. Jpn., 25(S1): 189-192. March 25, 2016 © The Acarological Society of Japan http://www.acarology-japan.org/ 189 Ticks and mites from a wild bird survey performed by the Wild Animal Medical Center of Rakuno Gakuen University in Japan 1, 2 3 2, 3 Tomoo YOSHINO , Kii USHIYAMA and Mitsuhiko ASAKAWA * 1Kushiro Zoo, Kushiro, Hokkaido 085-0201, Japan 2Wild Animal Medical Center, Graduate School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan 3Department of Pathobiology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan ABSTRACT A summary of avian ticks and mites from an epidemiological survey performed by the Wild Animal Medical Center of the Graduate School of Veterinary Medicine of Rakuno Gakuen University is reported, with errata for mite taxa. Ten taxa were recorded, and their taxonomic positions are shown in a table. Key words: ticks, mites, birds, Wild Animal Medical Center, Japan Epidemiological surveys are an essential conservation tool for fully understanding infectious diseases. In 2004, we began an investigation of avian parasitic diseases at the Wild Animal Medical Center of the Graduate School of Veterinary Medicine of Rakuno Gakuen University, Japan (Asakawa 2010; Asakawa et al. 2002, 2013; Hirayama et al. 2013). This paper summarizes the findings of seven publications on avian ticks and parasitic mites (Nakamura et al. 2003; Uemura et al. 2010; Yoshino et al. 2003, 2009a, b, 2011, 2013). During submission of a publication for the proceedings of the 14th International Congress of Acarology, Kyoto, taxonomical inconsistencies, including invalid names or synonyms for mite species, were reported to us.
  • Journal of the Helminthological Society of Washington 63(2) 1996

    Journal of the Helminthological Society of Washington 63(2) 1996

    July 1996 Number 2 Of of Washington A semiannual journal of research devoted to Helminthology and all branches of Parasitology Supported in part by the Brayton H. Ransom Memorial Trust Fund D. C. KRITSKY, W. A. :B6EC3ER, AND M, JEGU. NedtropicaliMonogehoidea/lS. An- — cyrocephalinae (Dactylogyridae) of Piranha and Their Relatives (Teleostei, JSer- rasalmidae) from Brazil and French Guiana: Species of Notozothecium Boeger and Kritsky, 1988, and Mymarotheciumgem. n. ..__ _______ __, ________ ..x,- ______ .s.... A. KOHN, C. P. SANTOS, AND-B. LEBEDEV. Metacdmpiella euzeti gen. n., sp, n., and I -Hargicola oligoplites~(Hargis, 1951) (Monogenea: Allodiscpcotylidae) from Bra- " zilian Fishes . ___________ ,:...L".. _______ j __ L'. _______l _; ________ 1 ________ _ __________ ______ _ .' _____ . __.. 176 C. P. SANTO?, T. SOUTO-PADRGN, AND R. M. LANFREDI. Atriasterheterodus (Levedev and Paruchin, 1969) and Polylabris tubicimts (Papema and Kohn, 1964) (Mono- ' genea) from Diplodus argenteus (Val., 1830) (Teleostei: Sparidae) from Brazil 181 . I...N- CAIRA AND T. BARDOS. Further Information on :.Gymnorhynchus isuri (Trypa- i/:norhyncha: Gymnorhynchidae) from the Shortfin Make Shark ...,.'. ..^_.-"_~ ____. ; 188 O. M. AMIN AND W. L.'MmcKLEY. Parasites of Some Fisji Introduced into an Arizona Reservoir, with Notes on Introductions — . ____ : ______ . ___.i;__ L____ _ . ______ :___ _ .193 O. M. AMIN AND O. SEY. Acanthocephala from Arabian Gulf Fishes off Kuwait, with 'Descriptions of Neoechinorhynchus dimorphospinus sp. n. XNeoechinorhyrichi- dae), Tegorhyrichus holospinosus sp. n. (l\lio&&ntid&e),:Micracanthorynchina-ku- waitensis sp. n. (Rhadinorhynchidae), and Sleriidrorhynchus breviclavipraboscis gen. n., sp. p. (Diplosentidae); and Key to Species of the Genus Micracanthor- .
  • Ptilonyssus Echinatus Berlese Y Trouessart, 1889

    Ptilonyssus Echinatus Berlese Y Trouessart, 1889

    PTILONYSSUS ECHINATUS BERLESE Y TROUESSART, 1889 (ACARI, MESOSTIGMATA, RHINONYSSIDAE) NUEVO PARA LA FAUNA ESPAÑOLA: ESTUDIO MORFO-BIOMETRICO DE LA DEUTONINFA Y LA PROTONINFA M. B. Rodríguez, J. M. Ubeda, D. C. Guevara y C. Ariza * RESUMEN Se presenta un estudio morfo-biométrico y una primera cita en España de las hembras de Ptilonyssus echinatus Berlese y Trouessart, 1889, utilizando dibujos de la morfología dor- sal y ventral así como una biometría completa. Asimismo, se incluye una amplia descrip- ción morfológica y biométrica (nueva para la ciencia) de la deutoninfa y protoninfa de esta especie. Los ácaros fueron recolectados de las fosas nasales de Hirundo rustica L. y Deli- chon urbica (L.) (Aves, Passeriformes, Hirundinidae) capturadas en la provincia de Gra- nada (España). El estudio de la variabilidad morfológica de los ejemplares obtenidos en Hirundo rustica y los de Delichon urbica nos lleva a pensar que las diferencias existentes entre ellos son sólo el producto de una gran variabilidad de la especie, que presenta tipos morfológicos distintos según el hospedador que parasita y la procedencia geográfica del mis- mo, y por tanto, se considera que dichas diferencias morfológicas en ningún caso pueden llevar a la creación de nuevas especies o subespecies tal como habían indicado algunos auto- res. pará- Palabras clave: Ptilonyssus echinatus, Acari, Rhinonyssidae, variabilidad, biometría, sitos nasales, aves, protoninfa, deutoninfa, España. ABSTRACT Ptilonyssus echinatus Berlese & Trouessart, 1889 (Acari, Mesostigmata, Rhinonyssidae), a new mite for the Spanish fauna: a morpho-biometrical study of deutonymph and pro- tonymph. We present a morpho-biometrical study and the first record for Spain of females of Pti- lonyssus echinatus Berlese & Trouessart, 1889, using ventral and dorsal drawings and enti- re biometry.
  • Terrestrial Arthropods)

    Terrestrial Arthropods)

    Fall 2004 Vol. 23, No. 2 NEWSLETTER OF THE BIOLOGICAL SURVEY OF CANADA (TERRESTRIAL ARTHROPODS) Table of Contents General Information and Editorial Notes..................................... (inside front cover) News and Notes Forest arthropods project news .............................................................................51 Black flies of North America published...................................................................51 Agriculture and Agri-Food Canada entomology web products...............................51 Arctic symposium at ESC meeting.........................................................................51 Summary of the meeting of the Scientific Committee, April 2004 ..........................52 New postgraduate scholarship...............................................................................59 Key to parasitoids and predators of Pissodes........................................................59 Members of the Scientific Committee 2004 ...........................................................59 Project Update: Other Scientific Priorities...............................................................60 Opinion Page ..............................................................................................................61 The Quiz Page.............................................................................................................62 Bird-Associated Mites in Canada: How Many Are There?......................................63 Web Site Notes ...........................................................................................................71
  • Redalyc.First Record of the Nasal Mite Rallinyssus Caudistigmus

    Redalyc.First Record of the Nasal Mite Rallinyssus Caudistigmus

    Revista de la Sociedad Entomológica Argentina ISSN: 0373-5680 [email protected] Sociedad Entomológica Argentina Argentina KUN, MARCELO E.; VEGA, ROCÍO M. First record of the nasal mite Rallinyssus caudistigmus Strandtmann (Acari: Rhinonyssidae) from Argentina Revista de la Sociedad Entomológica Argentina, vol. 75, núm. 1-2, 2016, pp. 97-100 Sociedad Entomológica Argentina Buenos Aires, Argentina Available in: http://www.redalyc.org/articulo.oa?id=322046181008 How to cite Complete issue Scientific Information System More information about this article Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Journal's homepage in redalyc.org Non-profit academic project, developed under the open access initiative Nota Científica Scientific Note ISSN 0373-5680 (impresa), ISSN 1851-7471 (en línea) Revista de la Sociedad Entomológica Argentina 75 (1-2): 97-100, 2016 First record of the nasal mite Rallinyssus caudistigmus Strandtmann (Acari: Rhinonyssidae) from Argentina KUN, Marcelo1 E. & Rocío M. VEGA2 1Laboratorio de Zoología, Centro Regional Universitario Bariloche, Universidad Nacional del Co- mahue, Quintral 1250, Bariloche Río Negro, Argentina. E-mail: [email protected] 2Laboratorio de Parasitología, INIBIOMA (UNCo-CONICET), Quintral 1250, Bariloche Río Ne- gro, Argentina. First record of the nasal mite Rallinyssus caudistigmus Strandtmann (Acari: Rhinonyssidae) from Argentina RESUMEN. Durante el estudio de la parasitofauna de la gallareta de ligas rojas Fulica armillata del Lago Mari Menuco en el noroeste de la Patagonia argentina, se encon- traron ejemplares de ácaros nasales pertenecientes a Rallinyssus caudistigmus. Este es el registro más austral de R. caudistigmus en F. armillata como nuevo hospedador. PALABRAS CLAVE. Ácaros Rhinonyssidae.
  • Parasitic Helminths and Arthropods of Fulvous Whistling-Ducks (Dendrocygna Bicolor) in Southern Florida

    Parasitic Helminths and Arthropods of Fulvous Whistling-Ducks (Dendrocygna Bicolor) in Southern Florida

    J. Helminthol. Soc. Wash. 61(1), 1994, pp. 84-88 Parasitic Helminths and Arthropods of Fulvous Whistling-Ducks (Dendrocygna bicolor) in Southern Florida DONALD J. FORRESTER,' JOHN M. KINSELLA,' JAMES W. MERTiNS,2 ROGER D. PRICE,3 AND RICHARD E. TuRNBULL4 5 1 Department of Infectious Diseases, College of Veterinary Medicine, University of Florida, Gainesville, Florida 32610, 2 U.S. Department of Agriculture, Animal and Plant Health Inspection Service, Veterinary Services, National Veterinary Services Laboratories, P.O. Box 844, Ames, Iowa 50010, 1 Department of Entomology, University of Minnesota, St. Paul, Minnesota 55108, and 4 Florida Game and Fresh Water Fish Commission, Okeechobee, Florida 34974 ABSTRACT: Thirty fulvous whistling-ducks (Dendrocygna bicolor) collected during 1984-1985 from the Ever- glades Agricultural Area of southern Florida were examined for parasites. Twenty-eight species were identified and included 8 trematodes, 6 cestodes, 1 nematode, 4 chewing lice, and 9 mites. All parasites except the 4 species of lice and 1 of the mites are new host records for fulvous whistling-ducks. None of the ducks were infected with blood parasites. Every duck was infected with at least 2 species of helminths (mean 4.2; range 2- 8 species). The most common helminths were the trematodes Echinostoma trivolvis and Typhlocoelum cucu- merinum and 2 undescribed cestodes of the genus Diorchis, which occurred in prevalences of 67, 63, 50, and 50%, respectively. Only 1 duck was free of parasitic arthropods; each of the other 29 ducks was infested with at least 3 species of arthropods (mean 5.3; range 3-9 species). The most common arthropods included an undescribed feather mite (Ingrassia sp.) and the chewing louse Holomenopon leucoxanthum, both of which occurred in 97% of the ducks.
  • COOPERATIVE NATIONAL PARK RESOURCES STUDIES UNIT UNIVERSITY of HAWAII at MANOA Department of Botany Honolulu, Hawaii 96822 (808) 948-8218 Clifford W

    COOPERATIVE NATIONAL PARK RESOURCES STUDIES UNIT UNIVERSITY of HAWAII at MANOA Department of Botany Honolulu, Hawaii 96822 (808) 948-8218 Clifford W

    COOPERATIVE NATIONAL PARK RESOURCES STUDIES UNIT UNIVERSITY OF HAWAII AT MANOA Department of Botany Honolulu, Hawaii 96822 (808) 948-8218 Clifford W. Smith, Unit Director Associate Professor of Botany Technical Report 29 MITES (CHELICERATA: ACARI) PARASITIC ON BIRDS IN HAWAII VOLCANOES NATIONAL PARK Technical Report 30 DISTRIBUTION OF MOSQUITOES (DIPTERA: CULICIDAE) ON THE EAST FLANK OF MAUNA LOA VOLCANO, HAWAI'I M. Lee Goff February 1980 UNIVERSITY OF HAWAII AT MANOA NATIONAL PARK SERVICE Contract No. CX 8000 7 0009 Contribution Nos. CPSU/UH 022/7 and CPSU/UH 022/8 MITES (CHELICERATA: ACARI) PARASITIC ON BIRDS IN HAWAII VOLCANOES NATIONAL PARK M. Lee Goff Department of Entomology B. P. Bishop Museum P. 0. Box 6037 Honolulu, Hawaii 96818 ABSTRACT The external parasites of native and exotic birds captured in Hawaii Volcanoes National Park are recorded. Forty-nine species of mites in 13 families were recovered from 10 species of birds. First records of Harpyrhynchidae are given for 'Amakihi and 'Apapane; Cytodites sp. (Cytoditidae) is recorded from the Red-b'illed Leiothrix for the first time in Hawaili. Two undescribed species of Cheyletiellidae, 1 undescribed species of Pyroglyphidae, and 19 undescribed feather mites of the super- family Analgoidea are noted. RECOMMENDATIONS Information presented in this report is primarily of a pre- liminary nature due to the incomplete state of the taxonomy of mites. This data will add to the basic knowledge of the stress placed on the bird populations within the Park. The presence of Ornithonyssus sylviarum in collections made of the House Finch provides a potential vector for viral and other diseases of birds, including various encephalides and Newcastles Disease.
  • MORFOLOGIA E DESENVOLVIMENTO DO SISTEMA REPRODUTOR MASCULINO DE CARRAPATOS DO GÊNERO Amblyomma (ACARI, IXODIDAE): UMA ANÁLISE COMPARATIVA

    MORFOLOGIA E DESENVOLVIMENTO DO SISTEMA REPRODUTOR MASCULINO DE CARRAPATOS DO GÊNERO Amblyomma (ACARI, IXODIDAE): UMA ANÁLISE COMPARATIVA

    UNIVERSIDADE ESTADUAL PAULISTA “JÚLIO DE MESQUITA FILHO” INSTITUTO DE BIOCIÊNCIAS – RIO unesp CLARO PROGRAMA DE PÓS-GRADUAÇÃO EM CIÊNCIAS BIOLÓGICAS (BIOLOGIA CELULAR E MOLECULAR) MORFOLOGIA E DESENVOLVIMENTO DO SISTEMA REPRODUTOR MASCULINO DE CARRAPATOS DO GÊNERO Amblyomma (ACARI, IXODIDAE): UMA ANÁLISE COMPARATIVA BRUNO RODRIGUES SAMPIERI Tese apresentada ao Instituto de Biociências do Campus de Rio Claro, Universidade Estadual Paulista, como parte dos requisitos para obtenção do título de Doutor em Ciências Biológicas (Biologia Celular e Molecular). Maio - 2016 UNIVERSIDADE ESTADUAL PAULISTA “JÚLIO DE MESQUITA FILHO” INSTITUTO DE BIOCIÊNCIAS – RIO unesp CLARO PROGRAMA DE PÓS-GRADUAÇÃO EM CIÊNCIAS BIOLÓGICAS (BIOLOGIA CELULAR E MOLECULAR) MORFOLOGIA E DESENVOLVIMENTO DO SISTEMA REPRODUTOR MASCULINO DE CARRAPATOS DO GÊNERO Amblyomma (ACARI, IXODIDAE): UMA ANÁLISE COMPARATIVA BRUNO RODRIGUES SAMPIERI ORIENTADORA: PROFA. DRA. MARIA IZABEL CAMARGO-MATHIAS CO-ORIENTADOR: PROF. DR. ODAIR CORREA BUENO Tese apresentada ao Instituto de Biociências do Campus de Rio Claro, Universidade Estadual Paulista, como parte dos requisitos para obtenção do título de Doutor em Ciências Biológicas (Biologia Celular e Molecular). Maio - 2016 591.4 Sampieri, Bruno Rodrigues S192m Morfologia e desenvolvimento do sistema reprodutor masculino de carrapatos do gênero Amblyomma (Acari, Ixodidae) : uma análise comparativa / Bruno Rodrigues Sampieri. - Rio Claro, 2016 113 f. : il., figs., tabs. Tese (doutorado) - Universidade Estadual Paulista, Instituto de Biociências de Rio Claro Orientadora: Maria Izabel Souza Camargo Coorientador: Odair Correa Bueno 1. Anatomia comparada. 2. Espermiotaxonomia. 3. Espermiocladística. 4. Filogenia. 5. Controle. I. Título. Ficha Catalográfica elaborada pela STATI - Biblioteca da UNESP Campus de Rio Claro/SP Dedicatória Dedico esta tese aos meus pais, à minha esposa, à minha filha e ao meu irmão, pelo apoio, companheirismo e paciência; por compartilharem comigo angústias e conquistas.
  • Page 1 Acarorum Catalogus VI 1 ACARORUM CATALOGUS Editor

    Page 1 Acarorum Catalogus VI 1 ACARORUM CATALOGUS Editor

    Acarorum Catalogus VI 1 ACARORUM CATALOGUS Editor Petar Beron 2 Acarorum Catalogus VI Acarorum Catalogus VI 3 ACARORUM CATALOGUS VI Order Mesostigmata Gamasina: Dermanyssoidea (Rhinonyssidae, Spinturnicidae) Petar Beron Pensoft National Museum of Natural History, Sofia Bulgarian Academy of Sciences Sofia 2020 4 Acarorum Catalogus VI ACARORUM CATALOGUS VI Order Mesostigmata. Gamasina: Dermanyssoidea (Rhinonyssidae, Spinturnicidae) Petar Beron First published 2020 ISBN 978-619-248-005-9 (hardback) ISBN 978-619-248-006-6 (e-book) Pensoft Series Faunistica No 220 Beron P. 2020. Acarorum Catalogus VI. Order Mesostigmata. Gamasina: Dermanyssoidea (Rhinonyssidae, Spinturnicidae). Pensoft & Natn. Mus. Nat. Hist., Sofia, 265 pp. © PENSOFT Publishers © National Museum of Natural History, Sofia All rights reserved. No part of this publication may be reproduced, stored in a retrieval system or transmitted in any form by any means, electronic, mechanical, photocopying, recording or otherwise, without the prior written permission of the copyright owner. Pensoft Publishers Prof. Georgi Zlatarski 12, Sofia 1700, Bulgaria Fax: +359-2-870-42-82 [email protected] www.pensoft.net Printed in Bulgaria, June 2020 Acarorum Catalogus VI 5 Contents Superorder Acariformes ...........................................................................................................7 Order Mesostigmata G. Canestrini, 1891 .......................................................................7 Suborder Monogynaspida Camin et Gorirossi, 1955 ..................................................7
  • MELLIS, ANNA MARIE MS Spatial Variation in Mammal And

    MELLIS, ANNA MARIE MS Spatial Variation in Mammal And

    MELLIS, ANNA MARIE M.S. Spatial Variation in Mammal and Ectoparasite Communities in the Foothills along the Southern Appalachian Mountains. (2021) Directed by Dr. Bryan McLean. 66 pp. Small mammal and ectoparasite community variation and abundance is important for monitoring the transmission rate of zoonotic diseases and informing conservation efforts that maintain host and parasite biodiversity in ecosystems facing global climate change. The purpose of this study was to identify the factors driving variation in small mammal and ectoparasite communities in the Southern Appalachian Mountains. I took an approach to sampling that allowed me to test predictions from island biogeography theory; namely, that host species richness varies with distance from the main Appalachian mountain range. I also examined how ectoparasite species richness varied with small mammal richness as well as ecological variables. Finally, I analyzed ectoparasite abundances at the community- and individual-host levels to understand how changes in host species richness may affect infestation rates. Comprehensive field surveys and ectoparasite screenings were performed across four field sites, two isolated from the Southern Appalachian Mountains and two along the Southern Appalachian Mountains. I found that these field sites were characterized by a mix of high and low elevation mammal species, and that community structure varied with degree of isolation for mammals, but not ectoparasites. Habitat type was a significant driver of species variation within and among sites. I found decreased abundances in ectoparasite compound communities when host species diversity was highest, which is consistent with predictions from a dilution effect. However, when evaluating abundances of individual ectoparasites, only one (Leptotrombidium peromysci) of four species displayed patterns consistent a dilution effect.
  • The Case of Feather Mites

    The Case of Feather Mites

    On the diversification of highly host-specific symbionts: the case of feather mites Jorge Doña On the diversification of highly host-specific symbionts: the casePhD Thesis of feather mites Recommended citation: Doña, J. (2018) On the diversification of highly host-specific symbionts: the case of feather mites. PhD Thesis. Universidad de Sevilla. Spain. On the diversification of highly host-specific symbionts: the case of feather mites Memoria presentada por el Licenciado en Biología y Máster en Genética y Evolución Jorge Doña Reguera para optar al título de Doctor por la Universidad de Sevilla Fdo. Jorge Doña Reguera Conformidad de los directores: Director Director Fdo.: Dr. Roger Jovani Tarrida Fdo.: Dr. David Serrano Larraz Tutor Fdo.: Dr. Manuel Enrique Figueroa Clemente 4 List of works derived from this Ph.D. thesis: - Chapter 1: Doña, J.*, Proctor, H.*, Mironov, S.*, Serrano, D., and Jovani, R. (2016). Global associations between birds and vane-dwelling feather mites. Ecology, 97, 3242. - Chapter 2: Doña, J., Diaz‐Real, J., Mironov, S., Bazaga, P., Serrano, D., & Jovani, R. (2015). DNA barcoding and mini‐barcoding as a powerful tool for feather mite studies. Molecular Ecology Resources, 15, 1216-1225. - Chapter 3: Vizcaíno, A.*, Doña, J.*, Vierna, J., Marí-Mena, N., Esteban, R., Mironov, S., Urien, C., Serrano, D., Jovani, R. Enabling large-scale feather mite studies: An Illumina DNA metabarcoding pipeline (under review in Experimental and Applied Acarology). - Chapter 4: Doña, J., Potti, J., De la Hera, I., Blanco, G., Frias, O., and Jovani, R. (2017). Vertical transmission in feather mites: insights into its adaptive value. Ecological Entomology, 42, 492-499.
  • Check List Lists of Species Check List 12(6): 2000, 22 November 2016 Doi: ISSN 1809-127X © 2016 Check List and Authors

    Check List Lists of Species Check List 12(6): 2000, 22 November 2016 Doi: ISSN 1809-127X © 2016 Check List and Authors

    12 6 2000 the journal of biodiversity data 22 November 2016 Check List LISTS OF SPECIES Check List 12(6): 2000, 22 November 2016 doi: http://dx.doi.org/10.15560/12.6.2000 ISSN 1809-127X © 2016 Check List and Authors New records of feather mites (Acariformes: Astigmata) from non-passerine birds (Aves) in Brazil Luiz Gustavo de Almeida Pedroso* and Fabio Akashi Hernandes Universidade Estadual Paulista, Departamento de Zoologia, Av. 24-A, 1515, 13506-900, Rio Claro, SP, Brazil * Corresponding author. E-mail: [email protected] Abstract: We present the results of our investigation of considerably enhances the mite diversity which can be feather mites (Astigmata) associated with non-passerine found in a single bird species. birds in Brazil. The studied birds were obtained from While most cases of feather mite transfer occur by roadkills, airport accidents, and from capitivity. Most physical contact between birds of the same species ectoparasites were collected from bird specimens by (e.g., during parental care and copulation), which often washing. A total of 51 non-passerine species from 20 links the evolutionary path of the groups and may to families and 15 orders were examined. Of them, 24 some extent mirror their phylogenetic trees (Dabert species were assessed for feather mites for the first time. and Mironov 1999; Dabert 2005), there are exceptional In addition, 10 host associations are recorded for the cases of interspecific tranfers that are poorly understood first time in Brazil. A total of 101 feather mite species (Mironov and Dabert 1999; Hernandes et al. 2014). were recorded, with 26 of them identified to the species Feather mites are often reported as ectocommensals, level and 75 likely representing undescribed species; living harmlessly on the bird’s body, feeding on the among the latter samples, five probably represent new uropygial oil produced by the birds (Blanco et al.