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Pediatric Cardiology & Healthcare Chuanxi Cai, Pediat Therapeut 2016, 6:5(Suppl) http://dx.doi.org/10.4172/2161-0665.C1.030 conferenceseries.com 2nd Global Congress and Expo on Pediatric Cardiology & Healthcare September 22-24, 2016 Las Vegas, USA Enhancing the effectiveness of human cardiac stem cell therapy with the HO-1 inducer-Cobalt Protoporphyrin (CoPP) Chuanxi Cai Albany Medical College, USA he regenerative potential of c-kit+ cardiac stem cells (CSCs) is severely limited by the poor survival of cells after Ttransplantation in the infracted heart. We have previously demonstrated that preconditioning human CSCs (hCSCs) with the HO-1 inducer, CoPP, has significant cytoprotective effects in vitro. Here, we examined whether preconditioning hCSCs with CoPP enhances CSC survival and improves cardiac function after transplantation in a model of myocardial infarction induced by a 45-min coronary occlusion and 35-day reperfusion in immunodeficient mice. At 30 min of reperfusion, CoPP- preconditioned hCSCsGFP+, hCSCsGFP+, or medium were injected into the border zone. Quantitative analysis with real time qPCR for the expression of the human specific gene HLA revealed that the number of survived hCSCs was significantly greater in the preconditioned-hCSC group at 24 hours, 7 and 35 days compared with the hCSC group. Co-immunostaining of tissue sections for both GFP and human nuclear antigen further confirmed greater hCSC numbers at 35 days in the preconditioned- hCSC group. At 35 days, compared with the hCSC group, the preconditioned-hCSC group exhibited increased positive and negative left ventricular (LV) dP/dt, end-systolic elastance and anterior wall/apical strain rate (although ejection fraction was similar), reduced LV remodeling, and increased proliferation of transplanted cells and of cells apparently committed to cardiac lineage. In conclusion, CoPP-preconditioning of hCSCs enhances their survival and/or proliferation, promotes greater proliferation of cells expressing cardiac markers, and results in greater improvement in LV remodeling and in indices of cardiac function after infarction. Biography Chuanxi Cai has completed his PhD from the Institute of Biophysics at Chinese Academy of Sciences in Beijing, China and Post-doctoral studies from UMDNJ- Robert Wood Johnson Medical School (RWJMS). Currently, he is the Associate Professor of Cardiovascular Medicine in the Albany Medical College. He has published 17 papers in reputed journals and has been serving as Editorial Board Member of several scientific journals. [email protected] Notes: Pediat Therapeut 2016 Volume 6 Issue 5(Suppl) ISSN: 2161-0665 Pediatrics, an open access journal Pediatric Cardiology 2016 September 22-24, 2016 Page 25 Bala Joshi, Pediat Therapeut 2016, 6:5(Suppl) http://dx.doi.org/10.4172/2161-0665.C1.030 conferenceseries.com 2nd Global Congress and Expo on Pediatric Cardiology & Healthcare September 22-24, 2016 Las Vegas, USA The role of the asthma educator in pediatric practices Bala Joshi Queens Hospital Center, USA he role of the asthma educator within a pediatric practice has been shown to enhance and improve patient outcomes. TData has shown exactly how much of a change occurs during a pediatric well child visit when there is the intervention of an education specialist. In the New York City database from the 379NYC Department of Health and Mental Hygiene, the percentage of pediatric patients hospitalized due to asthma exacerbations has declined from the high 80% to the lower 30% due to the introduction of an asthma educator providing extensive asthma education, teaching and reinforcement of all medications and devices during the well-child care visit. Additional data has shown a decrease in the number of school absences due to asthma, again upon the proactive intervention of an asthma educator within the pediatric practice. Before the year 2000, at Queens Hospital Center, studies have shown pre-asthma educator involvement lead to higher well-child visit hospital admissions, emergency department (ED) visits and missed days of school. Clearly, there was a need for more intervention. The NYC Department of Health and Mental Hygiene had started to recognize these high rates within their public hospitals and, therefore, established initiatives tailored to meet the needs of these patients and allow them to be more involved with their care. The NYC Asthma Initiative was presented to public hospital outpatient clinics with mandatory requirements at all well-child pediatric visits. Some of these requirements were: The introduction of a written asthma action plan at each visit; the completion of the medications administration form (MAF); the use of a chamber or spacer always with all prescribed inhalers, and; lastly, to have an asthma educator provide teaching and asthma education during the well-child visit. The asthma initiative along with the NYC Asthma Partnership (NYCAP) wanted to improve outcomes of pediatric asthmatic patients with the implementation of these mandatory requirements. Queens Hospital Center, in the year 2000, decided to implement an education specialist within their pediatric practice, in particular as an addition to their pediatric pulmonary clinic. The asthma educator would work with the provider, nurse and team to ensure a well-rounded educational experience for the asthmatic patient. Weekly visits with the introduction of an asthma educator during the well-child visit had started to show more improvements in declining hospitalization rates, ED visits for asthma and school absences. This data was evident over the years by the NYC Department of Health and Mental Hygiene, citywide, as well as, borough to borough. Patients now became more empowered and more connected with their condition, and knew they could always contact the asthma educator for any services they needed. There was also more compliance to keeping all their appointments and follow-up with their providers. Over the years, we can say that the impact of having an education specialist within a practice has shown great outcomes and has shown sustainability as well. Biography Bala Joshi has completed her BA from Queens College, 1987 and RRT in Respiratory Therapy from NYU School of Graduate Studies, 1994. Currently, she is working at Queens Hospital Center, USA. She is a Pediatric Health Educator for the last 16 years. She has been a member of many organizations and associations and has been a consistent team player within a pediatric practice. [email protected] Notes: Pediat Therapeut 2016 Volume 6 Issue 5(Suppl) ISSN: 2161-0665 Pediatrics, an open access journal Pediatric Cardiology 2016 September 22-24, 2016 Page 26 Andreas C Petropoulos, Pediat Therapeut 2016, 6:5(Suppl) http://dx.doi.org/10.4172/2161-0665.C1.030 conferenceseries.com 2nd Global Congress and Expo on Pediatric Cardiology & Healthcare September 22-24, 2016 Las Vegas, USA Bicuspid aortic valve- A defect of many clinical presentations and in any age Andreas C Petropoulos Azerbaijan State Medical University, Azerbaijan icuspid Aortic Valve (BAov) is the most common congenital heart defect (CHD). It’s prevalence rises from 0.5-2% of the Bgeneral population. This unique defect can create a variety of clinical conditions presenting from fetal to late adulthood. The aim of this paper is to highlight the many unique clinical presentations of a BAov. These range from nearby asymptomatic mild valve stenosis of incompetence to coactation of the Aorta, aneurysms, dissections of Ascending Aorta or cerebrovascular vessels and sever double valve disease in any age. The extreme spectrum of it can lead in fetal life in Hypoplastic Left heart Syndrome. BAov is commonly seen in Turner’s, William’s and Marfan’s Syndromes as well as related with VSD’s and coronary arteries abnormalities. The paper finally aims to alert the Pediatric Cardiology community on the long life surveillance that individuals with BAov need and address the variety of interventional and surgical techniques that can be used to address the many and in any age clinical presentations of it. Biography Andreas C Petropoulos graduated from Aristotle University’s Medical School, Greece in 1989. Following 30 year career as a Medical Officer, he joined as a senior Flight Surgeon in the Hellenic Air-Force. He has specialized in Aviation, Hyperbaric Medicine, Pediatrics, Fetal, Pediatrics and Congenital Cardiology in USA and Europe. He holds MSc in Preventive Cardiology. He is AEPC Prevention Working Group member. He has worked and lectured in Athens and Brussels universities. Currently, he consults in Fetal, Pediatrics, Congenital Cardiology in Merkezi Klinika and is Associate Professor at the State University and Post-graduate at CME Center in Azerbaijan. His research focuses on prevention, CVD imaging techniques, fetal cardiology and heart failure. [email protected] Notes: Pediat Therapeut 2016 Volume 6 Issue 5(Suppl) ISSN: 2161-0665 Pediatrics, an open access journal Pediatric Cardiology 2016 September 22-24, 2016 Page 27 Seliem Z S et al., Pediat Therapeut 2016, 6:5(Suppl) http://dx.doi.org/10.4172/2161-0665.C1.030 conferenceseries.com 2nd Global Congress and Expo on Pediatric Cardiology & Healthcare September 22-24, 2016 Las Vegas, USA Early identification of subtle left ventricular dysfunction among asymptomatic survivors of childhood acute myeloid leukemia: Insights from the novel three dimensional speckle tracking echocardiography Seliem Z S1,2, Abd E L Rahman M Y1, Helal M Y1, Sonia Magdy1,2, Rania M H El Kaffas1, Mohamed Saber2 and Mohamed Sabry2 1Cairo University Children Hospital, Egypt 2Children’s Cancer Hospital, Egypt Aim: To assess the value of novel three dimensional (3D) echocardiography and 3D speckle tracking echocardiography (3D-STE) for early detection of subtle LV myocardial dysfunction in asymptomatic survivors of childhood myeloid leukemia after anthracycline therapy. Methods: Thirty five survivors of childhood acute myeloid leukemia (mean age 13.5±4.4 years) treated with anthracycline and 23 age-matched controls were studied. The mean period of follow up after anthracylines therapy was 2.3±1.5 years. All patients and control underwent 3D echocardiography and 3D-STE to assess global longitudinal (GLS), circumferential (GCS) and area strain (GAS).
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